JP4890453B2 - 分離マトリックス及び精製法 - Google Patents

分離マトリックス及び精製法 Download PDF

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Publication number
JP4890453B2
JP4890453B2 JP2007518010A JP2007518010A JP4890453B2 JP 4890453 B2 JP4890453 B2 JP 4890453B2 JP 2007518010 A JP2007518010 A JP 2007518010A JP 2007518010 A JP2007518010 A JP 2007518010A JP 4890453 B2 JP4890453 B2 JP 4890453B2
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JP
Japan
Prior art keywords
ligand
buffer
gel
matrix
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2007518010A
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English (en)
Japanese (ja)
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JP2008503754A (ja
JP2008503754A5 (enExample
Inventor
マロワゼル，ジャン−リュック
ヨハンセン，ボ−レナート
フラット，ガンナー
ノーマン，ニルス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
GE Healthcare Bio Sciences Corp
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Publication of JP2008503754A5 publication Critical patent/JP2008503754A5/ja
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2007518010A 2004-06-24 2005-06-22 分離マトリックス及び精製法 Expired - Lifetime JP4890453B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0401665-5 2004-06-24
SE0401665A SE0401665D0 (sv) 2004-06-24 2004-06-24 Purification of immunoglobulins
PCT/SE2005/001002 WO2006001771A1 (en) 2004-06-24 2005-06-22 Separation matrix and method of purification

Publications (3)

Publication Number Publication Date
JP2008503754A JP2008503754A (ja) 2008-02-07
JP2008503754A5 JP2008503754A5 (enExample) 2008-08-14
JP4890453B2 true JP4890453B2 (ja) 2012-03-07

Family

ID=32733694

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2007518010A Expired - Lifetime JP4890453B2 (ja) 2004-06-24 2005-06-22 分離マトリックス及び精製法

Country Status (8)

Country Link
US (1) US8114280B2 (enExample)
EP (1) EP1759196B1 (enExample)
JP (1) JP4890453B2 (enExample)
CN (1) CN1972746B (enExample)
AU (1) AU2005257727B2 (enExample)
CA (1) CA2570624A1 (enExample)
SE (1) SE0401665D0 (enExample)
WO (1) WO2006001771A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090095676A1 (en) * 2005-05-16 2009-04-16 Masahiko Numata Carrier for Liquid Chromatography, Chromatographic Columns Packed With the Carrier, and Method of Separation of Organic Substances With the Columns
US20080299671A1 (en) * 2005-12-02 2008-12-04 Ge Healthcare Bio-Sciences Ab Hydrophobic Interaction Chromatography
JP2010515917A (ja) * 2007-01-10 2010-05-13 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー クロマトグラフィーレジン
WO2008121042A1 (en) * 2007-03-30 2008-10-09 Ge Healthcare Bio-Sciences Ab Purification of immunoglobulins
KR20130139153A (ko) * 2011-01-18 2013-12-20 백스터 인터내셔널 인코포레이티드 인간 혈액 중 항-베타 아밀로이드 항체의 측정
CN104812491B (zh) 2012-03-08 2018-06-19 生物辐射实验室股份有限公司 阴离子交换-疏水性混合模式
CN105579463B (zh) * 2014-03-14 2020-01-31 生物辐射实验室股份有限公司 混合模式配体
EP3116645B1 (en) * 2014-03-14 2019-04-10 GE Healthcare BioProcess R&D AB Separation matrices for purification of biological particles
CN104725559B (zh) * 2015-03-13 2017-06-06 安徽师范大学 嗜硫色谱材料及其制备方法和应用
US11065601B2 (en) * 2015-12-18 2021-07-20 University Of Canterbury Separation medium
EP4371663A3 (en) * 2016-04-06 2024-08-14 Cytiva BioProcess R&D AB Chromatography matrix
WO2017217930A1 (en) * 2016-06-15 2017-12-21 Agency For Science, Technology And Research A method of enhancing the performance of chromatography methods for purification of proteins
CN112480246A (zh) * 2020-12-22 2021-03-12 中国科学院过程工程研究所 一种犬免疫球蛋白的分离纯化方法及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01310744A (ja) * 1988-03-31 1989-12-14 Merck Patent Gmbh 分離材料
JPH06505624A (ja) * 1990-07-16 1994-06-30 バイオネブラスカ、インコーポレイテッド ペプチド類の精製指向性クローニング
JPH10506987A (ja) * 1994-09-23 1998-07-07 マッシー ユニヴァーシティー クロマトグラフ用樹脂およびその使用方法
JP2003514664A (ja) * 1999-11-22 2003-04-22 アメルシャム・バイオサイエンシーズ・アクチボラグ アニオン交換吸着方法およびアニオン交換体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US649826A (en) 1899-07-01 1900-05-15 Byron A Eldred Medicated hat-pad.
JPS6145773A (ja) 1984-08-07 1986-03-05 テルモ株式会社 体液浄化材
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
JP4425994B2 (ja) 1996-08-30 2010-03-03 ウプフロント クロマトグラフィー アクティーゼルスカブ 免疫グロブリンの単離
US6783711B2 (en) * 2000-05-23 2004-08-31 Ge Osmonics, Inc. Process for preparing a sulfonamide polymer matrix
CA2464086C (en) 2001-11-26 2011-01-18 Amersham Biosciences Ab Post-modification of a porous support
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01310744A (ja) * 1988-03-31 1989-12-14 Merck Patent Gmbh 分離材料
JPH06505624A (ja) * 1990-07-16 1994-06-30 バイオネブラスカ、インコーポレイテッド ペプチド類の精製指向性クローニング
JPH10506987A (ja) * 1994-09-23 1998-07-07 マッシー ユニヴァーシティー クロマトグラフ用樹脂およびその使用方法
JP2003514664A (ja) * 1999-11-22 2003-04-22 アメルシャム・バイオサイエンシーズ・アクチボラグ アニオン交換吸着方法およびアニオン交換体

Also Published As

Publication number Publication date
AU2005257727B2 (en) 2010-10-14
CN1972746B (zh) 2011-08-24
JP2008503754A (ja) 2008-02-07
US8114280B2 (en) 2012-02-14
EP1759196B1 (en) 2019-12-18
US20070196858A1 (en) 2007-08-23
EP1759196A1 (en) 2007-03-07
SE0401665D0 (sv) 2004-06-24
CN1972746A (zh) 2007-05-30
WO2006001771A1 (en) 2006-01-05
CA2570624A1 (en) 2006-01-05
AU2005257727A1 (en) 2006-01-05

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