CN1972746B - 分离基质和纯化方法 - Google Patents

分离基质和纯化方法 Download PDF

Info

Publication number
CN1972746B
CN1972746B CN2005800209726A CN200580020972A CN1972746B CN 1972746 B CN1972746 B CN 1972746B CN 2005800209726 A CN2005800209726 A CN 2005800209726A CN 200580020972 A CN200580020972 A CN 200580020972A CN 1972746 B CN1972746 B CN 1972746B
Authority
CN
China
Prior art keywords
antibody
matrix
ligand
eluent
amine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN2005800209726A
Other languages
English (en)
Chinese (zh)
Other versions
CN1972746A (zh
Inventor
J·-L·马洛塞尔
B·-L·约汉逊
G·格拉德
N·诺尔曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences Corp
Publication of CN1972746A publication Critical patent/CN1972746A/zh
Application granted granted Critical
Publication of CN1972746B publication Critical patent/CN1972746B/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN2005800209726A 2004-06-24 2005-06-22 分离基质和纯化方法 Expired - Lifetime CN1972746B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SE0401665-5 2004-06-24
SE04016655 2004-06-24
SE0401665A SE0401665D0 (sv) 2004-06-24 2004-06-24 Purification of immunoglobulins
PCT/SE2005/001002 WO2006001771A1 (en) 2004-06-24 2005-06-22 Separation matrix and method of purification

Publications (2)

Publication Number Publication Date
CN1972746A CN1972746A (zh) 2007-05-30
CN1972746B true CN1972746B (zh) 2011-08-24

Family

ID=32733694

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2005800209726A Expired - Lifetime CN1972746B (zh) 2004-06-24 2005-06-22 分离基质和纯化方法

Country Status (8)

Country Link
US (1) US8114280B2 (enExample)
EP (1) EP1759196B1 (enExample)
JP (1) JP4890453B2 (enExample)
CN (1) CN1972746B (enExample)
AU (1) AU2005257727B2 (enExample)
CA (1) CA2570624A1 (enExample)
SE (1) SE0401665D0 (enExample)
WO (1) WO2006001771A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006123576A1 (ja) * 2005-05-16 2006-11-23 National Institute Of Advanced Industrial Science And Technology 液体クロマトグラフィー用担体、該担体を充填したクロマトグラフィー用カラム、及び該カラムを用いた有機物の分離方法
WO2007064281A1 (en) * 2005-12-02 2007-06-07 Ge Healthcare Bio-Sciences Ab Hydrophobic interaction chromatography
JP2010515917A (ja) * 2007-01-10 2010-05-13 ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー クロマトグラフィーレジン
US9044740B2 (en) 2007-03-30 2015-06-02 Ge Healthcare Bio-Sciences Ab Purification of immunoglobulins
JP2014507649A (ja) * 2011-01-18 2014-03-27 バクスター・インターナショナル・インコーポレイテッド ヒト血液中の抗βアミロイド抗体の測定
WO2013134251A2 (en) * 2012-03-08 2013-09-12 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode
CN114917884A (zh) * 2014-03-14 2022-08-19 思拓凡生物工艺研发有限公司 用于生物颗粒纯化的分离基质
CN105579463B (zh) * 2014-03-14 2020-01-31 生物辐射实验室股份有限公司 混合模式配体
CN104725559B (zh) * 2015-03-13 2017-06-06 安徽师范大学 嗜硫色谱材料及其制备方法和应用
EP3389853B1 (en) * 2015-12-18 2023-10-18 University of Canterbury Separation medium
WO2017174422A1 (en) * 2016-04-06 2017-10-12 Ge Healthcare Bioprocess R&D Ab Chromatography matrix
CN109311937A (zh) * 2016-06-15 2019-02-05 新加坡科技研究局 增强用于纯化蛋白质的色谱法的性能的方法
CN112480246A (zh) * 2020-12-22 2021-03-12 中国科学院过程工程研究所 一种犬免疫球蛋白的分离纯化方法及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1200680A (zh) * 1995-09-20 1998-12-02 诺沃挪第克公司 新亲合配位体及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US649826A (en) 1899-07-01 1900-05-15 Byron A Eldred Medicated hat-pad.
JPS6145773A (ja) 1984-08-07 1986-03-05 テルモ株式会社 体液浄化材
DE3811042A1 (de) * 1988-03-31 1989-10-19 Merck Patent Gmbh Ionenaustauscher
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
JP4425994B2 (ja) * 1996-08-30 2010-03-03 ウプフロント クロマトグラフィー アクティーゼルスカブ 免疫グロブリンの単離
SE9904197D0 (sv) * 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
US6783711B2 (en) * 2000-05-23 2004-08-31 Ge Osmonics, Inc. Process for preparing a sulfonamide polymer matrix
US7060187B2 (en) 2001-11-26 2006-06-13 Ge Healthcare Bio-Sciences Ab Post-modification of a porous support
SE0303532D0 (sv) 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1200680A (zh) * 1995-09-20 1998-12-02 诺沃挪第克公司 新亲合配位体及其应用

Also Published As

Publication number Publication date
CN1972746A (zh) 2007-05-30
AU2005257727B2 (en) 2010-10-14
WO2006001771A1 (en) 2006-01-05
CA2570624A1 (en) 2006-01-05
EP1759196A1 (en) 2007-03-07
JP2008503754A (ja) 2008-02-07
US8114280B2 (en) 2012-02-14
JP4890453B2 (ja) 2012-03-07
AU2005257727A1 (en) 2006-01-05
SE0401665D0 (sv) 2004-06-24
US20070196858A1 (en) 2007-08-23
EP1759196B1 (en) 2019-12-18

Similar Documents

Publication Publication Date Title
JP4776615B2 (ja) 抗体精製
JP4848356B2 (ja) 抗体精製法
JP4831436B2 (ja) クロマトグラフィーリガンド
CN103269761B (zh) 亲和色谱基质
US8685248B2 (en) Purification of immunoglobulins
CN102272145B (zh) 亲和色谱基质
US9266041B2 (en) Chromatography ligand
CN1972746B (zh) 分离基质和纯化方法
US20080299671A1 (en) Hydrophobic Interaction Chromatography
CN101060931B (zh) 色谱配体
US9044740B2 (en) Purification of immunoglobulins
HK1213002B (zh) 陰離子交換-疏水性混合模式

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20160816

Address after: uppsala

Patentee after: GE HEALTHCARE BIO-SCIENCES AB

Address before: uppsala

Patentee before: GE HEALTHCARE BIO-SCIENCES AB

CP01 Change in the name or title of a patent holder

Address after: uppsala

Patentee after: Situofan Biotechnology R & D Co.,Ltd.

Address before: uppsala

Patentee before: GE HEALTHCARE BIO-SCIENCES AB

CP01 Change in the name or title of a patent holder
CX01 Expiry of patent term

Granted publication date: 20110824

CX01 Expiry of patent term