AU2005257727B2 - Separation matrix and method of purification - Google Patents

Separation matrix and method of purification Download PDF

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Publication number
AU2005257727B2
AU2005257727B2 AU2005257727A AU2005257727A AU2005257727B2 AU 2005257727 B2 AU2005257727 B2 AU 2005257727B2 AU 2005257727 A AU2005257727 A AU 2005257727A AU 2005257727 A AU2005257727 A AU 2005257727A AU 2005257727 B2 AU2005257727 B2 AU 2005257727B2
Authority
AU
Australia
Prior art keywords
matrix
antibodies
ligands
buffer
separation matrix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2005257727A
Other languages
English (en)
Other versions
AU2005257727A1 (en
Inventor
Gunnar Glad
Bo-Lennart Johansson
Jean-Luc Maloisel
Nils Norrman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences Corp
Publication of AU2005257727A1 publication Critical patent/AU2005257727A1/en
Application granted granted Critical
Publication of AU2005257727B2 publication Critical patent/AU2005257727B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3251—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AU2005257727A 2004-06-24 2005-06-22 Separation matrix and method of purification Ceased AU2005257727B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0401665A SE0401665D0 (sv) 2004-06-24 2004-06-24 Purification of immunoglobulins
SE0401665-5 2004-06-24
PCT/SE2005/001002 WO2006001771A1 (en) 2004-06-24 2005-06-22 Separation matrix and method of purification

Publications (2)

Publication Number Publication Date
AU2005257727A1 AU2005257727A1 (en) 2006-01-05
AU2005257727B2 true AU2005257727B2 (en) 2010-10-14

Family

ID=32733694

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005257727A Ceased AU2005257727B2 (en) 2004-06-24 2005-06-22 Separation matrix and method of purification

Country Status (8)

Country Link
US (1) US8114280B2 (enExample)
EP (1) EP1759196B1 (enExample)
JP (1) JP4890453B2 (enExample)
CN (1) CN1972746B (enExample)
AU (1) AU2005257727B2 (enExample)
CA (1) CA2570624A1 (enExample)
SE (1) SE0401665D0 (enExample)
WO (1) WO2006001771A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090095676A1 (en) * 2005-05-16 2009-04-16 Masahiko Numata Carrier for Liquid Chromatography, Chromatographic Columns Packed With the Carrier, and Method of Separation of Organic Substances With the Columns
US20080299671A1 (en) * 2005-12-02 2008-12-04 Ge Healthcare Bio-Sciences Ab Hydrophobic Interaction Chromatography
US8092683B2 (en) * 2007-01-10 2012-01-10 Ge Healthcare Bio-Sciences Ab Multi-modal ion exchange chromatography resins
US9044740B2 (en) 2007-03-30 2015-06-02 Ge Healthcare Bio-Sciences Ab Purification of immunoglobulins
WO2012099576A1 (en) * 2011-01-18 2012-07-26 Baxter International Inc. Measurement of anti-amyloid antibodies in human blood
CN104812491B (zh) 2012-03-08 2018-06-19 生物辐射实验室股份有限公司 阴离子交换-疏水性混合模式
WO2015138928A2 (en) * 2014-03-14 2015-09-17 Bio-Rad Laboratories, Inc. Mixed mode ligands
JP6702587B2 (ja) * 2014-03-14 2020-06-03 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ 生物粒子の精製用分離マトリックス
CN104725559B (zh) * 2015-03-13 2017-06-06 安徽师范大学 嗜硫色谱材料及其制备方法和应用
EP3389853B1 (en) * 2015-12-18 2023-10-18 University of Canterbury Separation medium
WO2017174422A1 (en) * 2016-04-06 2017-10-12 Ge Healthcare Bioprocess R&D Ab Chromatography matrix
US10927145B2 (en) * 2016-06-15 2021-02-23 Agency For Science, Technology And Research Method of enhancing the performance of chromatography methods for purification of proteins
CN112480246A (zh) * 2020-12-22 2021-03-12 中国科学院过程工程研究所 一种犬免疫球蛋白的分离纯化方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4725355A (en) * 1984-08-07 1988-02-16 Terumo Kabushiki Kaisha Body fluid purification medium and apparatus
US6702943B1 (en) * 1999-11-22 2004-03-09 Amersham Biosciences Ab Method for anion-exchange adsorption and anion-exchangers

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US649826A (en) 1899-07-01 1900-05-15 Byron A Eldred Medicated hat-pad.
DE3811042A1 (de) * 1988-03-31 1989-10-19 Merck Patent Gmbh Ionenaustauscher
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
GB9519197D0 (en) * 1995-09-20 1995-11-22 Affinity Chromatography Ltd Novel affinity ligands and their use
US6117996A (en) * 1995-09-20 2000-09-12 Novo Nordisk A/S Triazine based ligands and use thereof
AU729039C (en) 1996-08-30 2001-08-02 Upfront Chromatography A/S Isolation of immunoglobulins
US6783711B2 (en) 2000-05-23 2004-08-31 Ge Osmonics, Inc. Process for preparing a sulfonamide polymer matrix
AU2002353728B2 (en) 2001-11-26 2008-04-10 Ge Healthcare Bio-Sciences Ab Post-modification of a porous support
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4725355A (en) * 1984-08-07 1988-02-16 Terumo Kabushiki Kaisha Body fluid purification medium and apparatus
US6702943B1 (en) * 1999-11-22 2004-03-09 Amersham Biosciences Ab Method for anion-exchange adsorption and anion-exchangers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LIU Y. ET AL: 'Novel sulfamethazine ligand used for one-step purification of immunoglobulin G from human plasma' JOURNAL OF CHROMATOGRAPHY B vol. 792, no. 2, 25 July 2003, pages 177 - 185 *
NILSSON K. ET AL: 'p-Toluensesulfonyl Chloride as an Activating Agent of Agarose for the Preparation of Immobilized Affinity Ligands and Proteins' EUR. J. BIOCHEM. vol. 112, no. 2, 02 November 1980, pages 397 - 402 *
PENG L. ET AL: 'Evaluation of activation methods with cellulose beads for immunosorbent purification of immunoglobulins' JOURNAL OF BIOTECHNOLOGY vol. 5, no. 4, May 1987, pages 255 - 265 *

Also Published As

Publication number Publication date
SE0401665D0 (sv) 2004-06-24
JP4890453B2 (ja) 2012-03-07
US8114280B2 (en) 2012-02-14
CN1972746A (zh) 2007-05-30
US20070196858A1 (en) 2007-08-23
CN1972746B (zh) 2011-08-24
WO2006001771A1 (en) 2006-01-05
EP1759196A1 (en) 2007-03-07
EP1759196B1 (en) 2019-12-18
AU2005257727A1 (en) 2006-01-05
CA2570624A1 (en) 2006-01-05
JP2008503754A (ja) 2008-02-07

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FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired