JP4845307B2 - 立体基体を用いた検出用アレイ - Google Patents
立体基体を用いた検出用アレイ Download PDFInfo
- Publication number
- JP4845307B2 JP4845307B2 JP2001292362A JP2001292362A JP4845307B2 JP 4845307 B2 JP4845307 B2 JP 4845307B2 JP 2001292362 A JP2001292362 A JP 2001292362A JP 2001292362 A JP2001292362 A JP 2001292362A JP 4845307 B2 JP4845307 B2 JP 4845307B2
- Authority
- JP
- Japan
- Prior art keywords
- substrate
- nucleic acid
- acid probe
- array
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000758 substrate Substances 0.000 title claims description 140
- 238000001514 detection method Methods 0.000 title claims description 22
- 239000000523 sample Substances 0.000 claims description 30
- 239000000126 substance Substances 0.000 claims description 20
- 108020004707 nucleic acids Proteins 0.000 claims description 9
- 102000039446 nucleic acids Human genes 0.000 claims description 9
- 150000007523 nucleic acids Chemical class 0.000 claims description 9
- 239000003550 marker Substances 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 230000005389 magnetism Effects 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 239000013566 allergen Substances 0.000 claims description 2
- 239000000427 antigen Substances 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims description 2
- 239000005556 hormone Substances 0.000 claims description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 67
- 239000002853 nucleic acid probe Substances 0.000 description 67
- 238000006243 chemical reaction Methods 0.000 description 42
- 238000000034 method Methods 0.000 description 25
- 108020004414 DNA Proteins 0.000 description 22
- 238000009396 hybridization Methods 0.000 description 19
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- 239000007790 solid phase Substances 0.000 description 14
- 238000005406 washing Methods 0.000 description 14
- 239000003298 DNA probe Substances 0.000 description 7
- 108020003215 DNA Probes Proteins 0.000 description 6
- 238000003491 array Methods 0.000 description 6
- 238000009739 binding Methods 0.000 description 6
- 239000011521 glass Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000007689 inspection Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 230000027455 binding Effects 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000003100 immobilizing effect Effects 0.000 description 3
- 239000004065 semiconductor Substances 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical compound [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000004381 surface treatment Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 1
- 101100379079 Emericella variicolor andA gene Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000005354 coacervation Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- -1 or the like Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 229910021420 polycrystalline silicon Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000003746 surface roughness Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001292362A JP4845307B2 (ja) | 2000-09-25 | 2001-09-25 | 立体基体を用いた検出用アレイ |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000-291306 | 2000-09-25 | ||
| JP2000291306 | 2000-09-25 | ||
| JP2000291306 | 2000-09-25 | ||
| JP2001292362A JP4845307B2 (ja) | 2000-09-25 | 2001-09-25 | 立体基体を用いた検出用アレイ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002181819A JP2002181819A (ja) | 2002-06-26 |
| JP2002181819A5 JP2002181819A5 (https=) | 2008-11-13 |
| JP4845307B2 true JP4845307B2 (ja) | 2011-12-28 |
Family
ID=26600695
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001292362A Expired - Fee Related JP4845307B2 (ja) | 2000-09-25 | 2001-09-25 | 立体基体を用いた検出用アレイ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4845307B2 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4694952B2 (ja) * | 2005-11-17 | 2011-06-08 | 旭化成株式会社 | 標識付き試薬を用いた分析方法、分析装置 |
| AU2016200047A1 (en) * | 2015-01-07 | 2016-07-21 | Personal Genomics, Inc. | Oriented Loading Systems And Method For Orienting A Particle Loaded In A Well |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6324157A (ja) * | 1987-03-18 | 1988-02-01 | Olympus Optical Co Ltd | 酵素免疫自動測定機 |
| JPH03264863A (ja) * | 1990-03-15 | 1991-11-26 | Toshiba Corp | 遺伝子検出方法 |
| US6875620B1 (en) * | 1996-10-31 | 2005-04-05 | Agilent Technologies, Inc. | Tiling process for constructing a chemical array |
| JP3944996B2 (ja) * | 1998-03-05 | 2007-07-18 | 株式会社日立製作所 | Ｄｎａプローブアレー |
| FR2784189B3 (fr) * | 1998-10-05 | 2000-11-03 | Commissariat Energie Atomique | Biopuce et dispositif de lecture d'une biopuce comportant une pluralite de zones de reconnaissance moleculaire |
| JP3872227B2 (ja) * | 1999-02-26 | 2007-01-24 | 北斗科学産業株式会社 | 新規の生物学的チップ及び分析方法 |
| KR20020089323A (ko) * | 2000-01-10 | 2002-11-29 | 제노스펙트라 인코포레이티드 | 선형 프로브 캐리어 |
| WO2005064334A1 (ja) * | 2003-12-30 | 2005-07-14 | Universal Bio Research Co., Ltd. | 粒子三次元配列体を利用した反応容器及び反応装置 |
-
2001
- 2001-09-25 JP JP2001292362A patent/JP4845307B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002181819A (ja) | 2002-06-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8999726B2 (en) | Microfluidic interface for highly parallel addressing of sensing arrays | |
| US6716629B2 (en) | Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof | |
| Xu et al. | Protein and chemical microarrays—powerful tools for proteomics | |
| US20030064386A1 (en) | Probe array for detecting a target material using stereo-substrate | |
| CA2671866C (en) | Analysis chip and analysis method | |
| CZ15799A3 (cs) | Pevný nosič pro analytické měřicí metody, jeho výroba a použití | |
| US20210016283A1 (en) | Ultrahigh throughput protein discovery | |
| JP2002542487A (ja) | ポリペプチドのマイクロアレイ | |
| US7253006B2 (en) | Device and method for manufacturing bead array, and method for detecting target substance | |
| US20050106607A1 (en) | Biochip containing reaction wells and method for producing same and use thereof | |
| CA2245013C (en) | Analytical measurement method and its use | |
| EP1605264B1 (en) | An integrating analysis chip with minimized reactors and its application | |
| JP4845307B2 (ja) | 立体基体を用いた検出用アレイ | |
| JP2004037453A (ja) | アンカー装置としての多ピンプラットフォームの使用 | |
| JP4262512B2 (ja) | プローブ固相化反応アレイ | |
| WO2005064334A1 (ja) | 粒子三次元配列体を利用した反応容器及び反応装置 | |
| CA2260807C (en) | Solid supports for analytical measuring processes, a process for their preparation, and their use | |
| US20050282182A1 (en) | Reaction vessel and reaction apparatus comprising three-dimensional particle array | |
| JP2007285828A (ja) | 検体溶液の撹拌方法 | |
| O'Connor et al. | Protein chips and microarrays | |
| JP2008249677A (ja) | 液体導入用デバイス、固定ホルダおよび分析キット | |
| JP2007132866A (ja) | 反応アレイ | |
| JP2003247990A (ja) | 生化学的検査用の検査基板とこれに用いる微小基板 | |
| EP1298433A1 (en) | Device and method for the generation of arrays of substance mixtures by diffusion | |
| Murakami et al. | Random distribution of biomaterials as a handling method on microarray applied to PCR, biosensors and high through-put screening |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080925 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20080925 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20080926 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20100909 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100914 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20101115 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110208 |
|
| A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110411 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20110927 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20111011 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20141021 Year of fee payment: 3 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20141021 Year of fee payment: 3 |
|
| LAPS | Cancellation because of no payment of annual fees |