JP4805859B2 - 飼料添加剤 - Google Patents
飼料添加剤 Download PDFInfo
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- JP4805859B2 JP4805859B2 JP2007023700A JP2007023700A JP4805859B2 JP 4805859 B2 JP4805859 B2 JP 4805859B2 JP 2007023700 A JP2007023700 A JP 2007023700A JP 2007023700 A JP2007023700 A JP 2007023700A JP 4805859 B2 JP4805859 B2 JP 4805859B2
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- strain
- animal
- feed
- bacillus subtilis
- feed additive
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- 239000003674 animal food additive Substances 0.000 title claims description 31
- 241001465754 Metazoa Species 0.000 claims description 55
- 244000063299 Bacillus subtilis Species 0.000 claims description 24
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 24
- 230000012010 growth Effects 0.000 claims description 20
- 244000052616 bacterial pathogen Species 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 14
- 239000004480 active ingredient Substances 0.000 claims description 13
- 244000144972 livestock Species 0.000 claims description 6
- 230000002401 inhibitory effect Effects 0.000 claims description 4
- 244000144977 poultry Species 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 description 15
- 241000607142 Salmonella Species 0.000 description 12
- 241000287828 Gallus gallus Species 0.000 description 11
- 108020004465 16S ribosomal RNA Proteins 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 241001333951 Escherichia coli O157 Species 0.000 description 6
- 206010030113 Oedema Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 206010016952 Food poisoning Diseases 0.000 description 5
- 208000019331 Foodborne disease Diseases 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000009422 growth inhibiting effect Effects 0.000 description 5
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 241000271566 Aves Species 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- LFYJSSARVMHQJB-QIXNEVBVSA-N bakuchiol Chemical compound CC(C)=CCC[C@@](C)(C=C)\C=C\C1=CC=C(O)C=C1 LFYJSSARVMHQJB-QIXNEVBVSA-N 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000021050 feed intake Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000282818 Giraffidae Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000015580 Increased body weight Diseases 0.000 description 1
- 241000588653 Neisseria Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000283080 Proboscidea <mammal> Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 229940111685 dibasic potassium phosphate Drugs 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 239000013615 primer Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
バチルス・サブチルスJA‐ZK株は、千葉県の土壌から分離した。分離した菌株は、グラム陽性の桿菌であり、嫌気的条件下では細胞増殖活動が停止し、芽胞を形成するものであった。
SCDブイヨン培地を用いて、サルモネラ・エンテリティディスのみの単独培養区と、サルモネラとJA‐ZK株と混合した混合培養区を設定した。培養は37℃で48時間行い、24時間ごとにサルモネラ菌数を測定した。測定されたサルモネラ菌数を常用対数で表した結果を表1に示す。
SCDブイヨン培地を用いて、浮腫病由来大腸菌のみの単独培養区と、該大腸菌とJA‐ZK株と混合した混合培養区を設定した。培養は37℃で48時間行い、24時間ごとに大腸菌菌数を測定した。測定された浮腫病由来大腸菌数を常用対数で表した結果を表2に示す。
SCDブイヨン培地を用いて、大腸菌O157のみの単独培養区と、大腸菌O157とJA‐ZK株と混合した混合培養区を設定した。培養は37℃で48時間行い、24時間ごとに大腸菌菌数を測定した。測定された大腸菌数を常用対数で表した結果を表3に示す。
市販の鶏用飼料にJA‐ZK株を有効成分とする飼料添加剤を菌数として106個/gになるように混合し、サルモネラ・エンテリティディス培養液を生理食塩水に懸濁し、ゾンデを用いて被験鶏のそ嚢内に投与した。その後、定期的に該被験鶏の糞を採取し、DHL寒天培地を用いてサルモネラの菌数を測定した。測定されたサルモネラ菌数を常用対数で表した結果を図1に示す。
市販の豚用飼料にJA‐ZK株を有効成分とする飼料添加剤を菌数として106個/gになるように混合し、離乳後の子豚に給与した。2週後の増加体重と飼料摂取量を測定した。その結果を表4に示す。
市販の鶏用飼料に飼料添加剤を菌数として106個/gになるように混合し、初生のブロイラーに給与した。8週後の増体重と飼料摂取量を測定した。結果を表5に示す。
JA‐ZK株の16SリボソームRNAの部分塩基配列を解析した。JA‐ZK株の平板培地上の集落を釣菌してゲノムDNAを抽出した後に、鋳型を調製してPCRにより16SrRNAのうち5‘末端側約500bpの領域を増幅し、増幅された塩基配列をシーケンシングし部分塩基配列を得た。前記PCR増幅には菌類の16SリボソームRNAの部分塩基配列同定に広く用いられているMicroSeq 500 16S rDNA Bacterial Identification Kit(Applied Biosystems, CA, USA)を使用した。プライマーは前記キットに付属のオリゴヌクレオチド・プライマーを使用し、PCR条件は同キットのプロトコルに従って設定した。
Claims (12)
- バチルス・サブチルスJA‐ZK株(受託番号:FERM P-21100)を有効成分として含有する飼料添加剤。
- 前記バチルス・サブチルスJA‐ZK株が乾燥状態にある請求項1記載の飼料添加剤。
- 動物体内に存在する病原性細菌の増殖抑制用である請求項1又は2記載の飼料添加剤。
- 動物の体重増加用である請求項1又は2記載の飼料添加剤。
- 動物の飼料要求率改善用である請求項1又は2記載の飼料添加剤。
- 前記動物が家畜又は家禽である請求項3ないし5のいずれか1項に記載の飼料添加剤。
- 請求項1ないし6のいずれか1項に記載の飼料添加剤を含む飼料。
- 前記バチルス・サブチルスJA‐ZK株を菌数として104〜109 個/gになるように添加した請求項7記載の飼料。
- バチルス・サブチルスJA‐ZK株を菌数として105〜107個/gになるように添加した請求項7記載の飼料。
- バチルス・サブチルスJA‐ZK株(受託番号:FERM P-21100)を動物に給与することを含む、該動物体内の病原細菌の増殖を抑制する方法。
- バチルス・サブチルスJA‐ZK株(受託番号:FERM P-21100)を動物に給与することを含む、該動物の体重を増加させる方法。
- バチルス・サブチルスJA‐ZK株(受託番号:FERM P-21100)を動物に給与することを含む、該動物の飼料要求率を改善させる方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007023700A JP4805859B2 (ja) | 2007-02-02 | 2007-02-02 | 飼料添加剤 |
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JP2007023700A JP4805859B2 (ja) | 2007-02-02 | 2007-02-02 | 飼料添加剤 |
Publications (2)
Publication Number | Publication Date |
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JP2008187929A JP2008187929A (ja) | 2008-08-21 |
JP4805859B2 true JP4805859B2 (ja) | 2011-11-02 |
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JP2007023700A Active JP4805859B2 (ja) | 2007-02-02 | 2007-02-02 | 飼料添加剤 |
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JP (1) | JP4805859B2 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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FR2999191B1 (fr) * | 2012-12-12 | 2016-02-05 | Lesaffre & Cie | Souches probiotiques pour le traitement et/ou la prevention de la diarrhee |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59128334A (ja) * | 1983-01-13 | 1984-07-24 | Sanwa Kagaku Kenkyusho:Kk | 家畜・家禽飼料用添加剤 |
JPS6075238A (ja) * | 1983-09-29 | 1985-04-27 | Toshio Tsubaki | 家畜飼料 |
JP2528055B2 (ja) * | 1991-10-04 | 1996-08-28 | カルピス食品工業株式会社 | 鳥類飼料添加用腸内サルモネラ属細菌減少剤及びその使用法 |
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