JP4793601B2 - 微粒子の誘電泳動の強さ評価方法 - Google Patents
微粒子の誘電泳動の強さ評価方法 Download PDFInfo
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- JP4793601B2 JP4793601B2 JP2008525748A JP2008525748A JP4793601B2 JP 4793601 B2 JP4793601 B2 JP 4793601B2 JP 2008525748 A JP2008525748 A JP 2008525748A JP 2008525748 A JP2008525748 A JP 2008525748A JP 4793601 B2 JP4793601 B2 JP 4793601B2
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- 238000004720 dielectrophoresis Methods 0.000 title claims description 37
- 238000000034 method Methods 0.000 title description 17
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- 239000002245 particle Substances 0.000 claims description 116
- 230000005684 electric field Effects 0.000 claims description 25
- 238000005259 measurement Methods 0.000 claims description 18
- 238000001514 detection method Methods 0.000 claims description 16
- 230000001678 irradiating effect Effects 0.000 claims description 6
- 238000011156 evaluation Methods 0.000 claims description 5
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- 238000013508 migration Methods 0.000 description 11
- 230000005012 migration Effects 0.000 description 11
- 230000035945 sensitivity Effects 0.000 description 8
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 6
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
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- 238000004458 analytical method Methods 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
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- 239000004816 latex Substances 0.000 description 1
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- 229920002521 macromolecule Polymers 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4788—Diffraction
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N2015/0003—Determining electric mobility, velocity profile, average speed or velocity of a plurality of particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N2015/0038—Investigating nanoparticles
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- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
M.Washizu,S.Suzuki,O.Kurosawa,T.Nishizaka,T.Shnohara,"Molecular dielectophoresis of biopolymers",IEEE Trans.on Industry Applications,vol.30 835-843(1994) Nicolas G. Green and Hywel Morgan, "Dielectrophoresis of Submicrometer Latex Spheres.1. Experimental Results"J.Phys.Chem.B,vol.103,41-50,(1999) 鷲津正夫,川端智久,黒沢修,鈴木誠一 "誘電泳動による生体分子のハンドリング技術と分離技術への応用",電子情報通信学会論文誌 C Vol.J83−C No.1,pp1−8 2000年8月
(2)ビデオ観察では泳動力の定量化が難しい。
(3)更に、最大の困難は、ナノ粒子のような粒子径の小さい粒子の観察に適さないという事実がある。誘電泳動力が粒子径の3乗(すなわち微粒子の体積に比例)に比例するので、粒子径が小さいほど極度に誘電泳動力が小さくなり、検出が困難となる。実際、誘電泳動を扱った報告の殆どが、直径μm程度またはせいぜい100nm程度までで、数十nm以下の粒子の測定は困難であると思われる。
本発明はこのような実情に鑑みてなされたもので、ナノ粒子を含む微粒子の誘電泳動力を、蛍光体の付着等の前処理を施すことなく、定量的に評価することのできる微粒子の誘電泳動の強さの評価方法の提供をその課題としている。
2 電極対
21,22 電極
21a,22a 電極片
21b,22b 接続部
3 電極電源
4 照射光学系
5 検出光学系
6 装置制御及びデータ取込み・処理装置
Claims (1)
- 媒体中に粒子群を分散させた試料をセル中に収容した状態で、そのセル中に規則的に並ぶ交流電界の分布を形成することにより、媒体中で粒子群を誘電泳動させて当該粒子群の密度分布による回折格子を生成させるとともに、その密度分布による回折格子に対して測定光を照射することにより発生する回折光を検出する動作を、同一の試料に対して上記交流電界の周波数を相違させて複数回にわたって行い、その各回折光の検出結果の相違に基づいて、試料の誘電泳動強度もしくは誘電泳動感受性の周波数依存性を評価することを特徴とする誘電泳動の強さ評価方法。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2006/314221 WO2008010267A1 (fr) | 2006-07-19 | 2006-07-19 | procédé et appareil d'évaluation de LA résistance de diélectrophorèse de fines particules |
Publications (2)
Publication Number | Publication Date |
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JPWO2008010267A1 JPWO2008010267A1 (ja) | 2009-12-10 |
JP4793601B2 true JP4793601B2 (ja) | 2011-10-12 |
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JP2008525748A Expired - Fee Related JP4793601B2 (ja) | 2006-07-19 | 2006-07-19 | 微粒子の誘電泳動の強さ評価方法 |
Country Status (4)
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US (1) | US8313628B2 (ja) |
EP (1) | EP2042852B1 (ja) |
JP (1) | JP4793601B2 (ja) |
WO (1) | WO2008010267A1 (ja) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9283597B2 (en) * | 2002-12-02 | 2016-03-15 | Cfd Research Corporation | Miniaturized electrothermal flow induced infusion pump |
US20100149532A1 (en) * | 2007-05-18 | 2010-06-17 | Naoji Moriya | Method and apparatus for optical measurement |
WO2009019766A1 (ja) * | 2007-08-08 | 2009-02-12 | Shimadzu Corporation | 光学的測定装置およびその電極対 |
JP5109988B2 (ja) * | 2009-01-21 | 2012-12-26 | 株式会社島津製作所 | 粒子径測定装置及び粒子径測定方法 |
US9261448B2 (en) * | 2014-06-27 | 2016-02-16 | Shimadzu Corporation | Particle size distribution measuring apparatus |
US9962714B2 (en) | 2015-07-12 | 2018-05-08 | International Business Machines Corporation | Microchannel, microfluidic chip and method for processing microparticles in a fluid flow |
US10081015B2 (en) * | 2015-07-12 | 2018-09-25 | International Business Machines Corporation | Trapping at least one microparticle |
KR102102534B1 (ko) * | 2018-07-11 | 2020-04-23 | 주식회사 엑스와이지플랫폼 | 유전 전기 영동을 이용한 마이크로 전극 바이오 센서, 및 이를 이용한 생체물질 검출 방법 |
WO2021100514A1 (ja) * | 2019-11-19 | 2021-05-27 | 株式会社堀場製作所 | 粒子径分布測定装置、及び、粒子径分布測定方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2006064659A (ja) * | 2004-08-30 | 2006-03-09 | Shimadzu Corp | 光学的測定装置および光学的測定方法 |
JP2006162333A (ja) * | 2004-12-03 | 2006-06-22 | Shimadzu Corp | 光学的測定装置および光学的測定方法 |
JP2006162332A (ja) * | 2004-12-03 | 2006-06-22 | Shimadzu Corp | 光学的測定装置 |
Family Cites Families (2)
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US5993630A (en) * | 1996-01-31 | 1999-11-30 | Board Of Regents The University Of Texas System | Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation |
EP1785718B1 (en) * | 2004-08-30 | 2017-01-04 | Shimadzu Corporation | Optical measuring device and method, nanoparticle measuring method and device |
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2006
- 2006-07-19 EP EP06781228.9A patent/EP2042852B1/en not_active Expired - Fee Related
- 2006-07-19 WO PCT/JP2006/314221 patent/WO2008010267A1/ja active Application Filing
- 2006-07-19 JP JP2008525748A patent/JP4793601B2/ja not_active Expired - Fee Related
- 2006-07-19 US US12/374,048 patent/US8313628B2/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2006064659A (ja) * | 2004-08-30 | 2006-03-09 | Shimadzu Corp | 光学的測定装置および光学的測定方法 |
JP2006162333A (ja) * | 2004-12-03 | 2006-06-22 | Shimadzu Corp | 光学的測定装置および光学的測定方法 |
JP2006162332A (ja) * | 2004-12-03 | 2006-06-22 | Shimadzu Corp | 光学的測定装置 |
Also Published As
Publication number | Publication date |
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EP2042852A4 (en) | 2012-01-04 |
US20100012496A1 (en) | 2010-01-21 |
US8313628B2 (en) | 2012-11-20 |
EP2042852A1 (en) | 2009-04-01 |
WO2008010267A1 (fr) | 2008-01-24 |
EP2042852B1 (en) | 2017-04-19 |
JPWO2008010267A1 (ja) | 2009-12-10 |
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