JP4734498B2 - タンパク質−タンパク質相互作用を解析する方法 - Google Patents

タンパク質−タンパク質相互作用を解析する方法 Download PDF

Info

Publication number
JP4734498B2
JP4734498B2 JP2006518902A JP2006518902A JP4734498B2 JP 4734498 B2 JP4734498 B2 JP 4734498B2 JP 2006518902 A JP2006518902 A JP 2006518902A JP 2006518902 A JP2006518902 A JP 2006518902A JP 4734498 B2 JP4734498 B2 JP 4734498B2
Authority
JP
Japan
Prior art keywords
protein
protease
sequence encoding
nucleotide sequence
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2006518902A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007528713A (ja
JP2007528713A5 (enExample
Inventor
リー，ケヴィン，ジェイ
アクセル，リチャード
ストラップス，ウォルター
バーニア，ギラッド
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Columbia University in the City of New York
Original Assignee
Columbia University in the City of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Columbia University in the City of New York filed Critical Columbia University in the City of New York
Publication of JP2007528713A publication Critical patent/JP2007528713A/ja
Publication of JP2007528713A5 publication Critical patent/JP2007528713A5/ja
Application granted granted Critical
Publication of JP4734498B2 publication Critical patent/JP4734498B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9406—Neurotransmitters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
JP2006518902A 2003-07-09 2004-07-09 タンパク質−タンパク質相互作用を解析する方法 Expired - Lifetime JP4734498B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US48596803P 2003-07-09 2003-07-09
US60/485,968 2003-07-09
US51191803P 2003-10-15 2003-10-15
US60/511,918 2003-10-15
US56611304P 2004-04-27 2004-04-27
US60/566,113 2004-04-27
PCT/US2004/021887 WO2005007822A2 (en) 2003-07-09 2004-07-09 Method for assaying protein-protein interaction

Publications (3)

Publication Number Publication Date
JP2007528713A JP2007528713A (ja) 2007-10-18
JP2007528713A5 JP2007528713A5 (enExample) 2007-12-13
JP4734498B2 true JP4734498B2 (ja) 2011-07-27

Family

ID=34084516

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006518902A Expired - Lifetime JP4734498B2 (ja) 2003-07-09 2004-07-09 タンパク質−タンパク質相互作用を解析する方法

Country Status (9)

Country Link
US (3) US7049076B2 (enExample)
EP (2) EP1644734B1 (enExample)
JP (1) JP4734498B2 (enExample)
CN (1) CN1894581B (enExample)
AT (1) ATE534900T1 (enExample)
AU (1) AU2004257228B8 (enExample)
CA (1) CA2531698A1 (enExample)
IL (1) IL173023A0 (enExample)
WO (1) WO2005007822A2 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070224615A1 (en) * 2003-07-09 2007-09-27 Invitrogen Corporation Methods for assaying protein-protein interactions
CN1894581B (zh) 2003-07-09 2012-02-01 生命技术公司 检测蛋白-蛋白相互作用的方法
EP1893627A4 (en) * 2005-05-27 2009-11-11 Sentigen Biosciences Inc MULTIPLEXARRAY SUITABLE FOR THE INVESTIGATION OF PROTEIN-PROTEIN INTERACTIONS
US20090305898A1 (en) 2005-07-21 2009-12-10 Ilan Morad Methods and Compositions for Identifying a Peptide Having an Intermolecular Interaction With a Target of Interest
WO2007092938A2 (en) * 2006-02-08 2007-08-16 Invitrogen Corporation Cellular assays for signaling receptors
EP1996732A4 (en) 2006-03-02 2010-03-31 Uab Research Foundation RECOGNITION AND TREATMENT AND DISCOVERY OF REMEDIES AGAINST MYCOBACTERIAL DISEASES
EP2002021B1 (en) * 2006-03-16 2011-10-12 Life Technologies Corporation Methods for assaying protein-protein interaction
WO2007149807A1 (en) * 2006-06-20 2007-12-27 Invitrogen Corporation Methods for identifying modifiers of gpr1 activity
EP2078037A4 (en) * 2006-11-10 2010-01-27 Dimerix Bioscience Pty Ltd HETERODIMERS / OLIGOMERS OF THE RECEPTOR FOR THE THYROTROPIN RELEASING HORMONE OF THE OREXIN RECEPTOR
US9026409B2 (en) 2007-08-06 2015-05-05 Design Ready Controls, Inc. Systems, methods, and software for automated design and manufacturing of HVAC control panels
WO2009032716A1 (en) * 2007-09-04 2009-03-12 Sanofi-Aventis Identifying molecules that modulate protein- protein interactions using protease activated reporters
FR2934684B1 (fr) 2008-07-31 2012-11-16 Cis Bio Int Methode de detection de l'internalisation de proteines membranaires.
CA2737669A1 (en) * 2008-09-25 2010-04-01 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Combinatorial synthesis and use of libraries of short expressed nucleic acid sequences for the analysis of cellular events
JP5586285B2 (ja) * 2009-03-18 2014-09-10 オリンパス株式会社 受容体相互作用検出方法およびサイクリックａｍｐセンサータンパク質
CN107899012A (zh) 2011-01-11 2018-04-13 戴麦里克斯生物科学有限公司 联合疗法
GB201103453D0 (en) 2011-03-01 2011-04-13 Vib Vzw Kinase substrate sensor
TW201302779A (zh) 2011-04-13 2013-01-16 Glaxosmithkline Biolog Sa 融合蛋白質及組合疫苗
WO2013049398A2 (en) 2011-09-28 2013-04-04 H. Lee Moffitt Cancer Center & Research Institute, Inc. Protein-protein interaction as biomarkers
WO2014153044A1 (en) * 2013-03-14 2014-09-25 Temple University Of The Commonwealth System Of Higher Education Treatment of patients with hyponatremia and cardiac disease
US10226528B2 (en) * 2013-03-15 2019-03-12 The Trustees Of The University Of Pennsylvania Vaccines with biomolecular adjuvants
GB201310008D0 (en) 2013-06-05 2013-07-17 Glaxosmithkline Biolog Sa Immunogenic composition for use in therapy
EP3103900B1 (en) * 2014-02-07 2020-09-09 Korea Research Institute Of Bioscience And Biotechnology High-throughput analysis method of interaction between materials, using protein fusion display technique
US10619155B2 (en) * 2015-03-17 2020-04-14 Brown University Methods for labeling and manipulating a cellular circuit
CN104991072B (zh) * 2015-06-16 2017-03-29 西北农林科技大学 一种昆虫体外蛋白质相互作用检测系统的制备方法及应用
CN108463229B (zh) 2016-01-11 2023-10-17 斯坦福大学托管董事会 嵌合蛋白和免疫治疗方法
CN109312335B (zh) 2016-01-11 2023-10-24 斯坦福大学托管董事会 嵌合蛋白和调节基因表达的方法
GB201609604D0 (en) * 2016-06-01 2016-07-13 Ucl Business Plc Cell
CN107976411B (zh) * 2016-10-25 2020-10-23 中国科学院微生物研究所 包含别构转录因子调控系统的生物传感器、试剂盒及其在小分子检测中的用途
WO2019062744A1 (zh) * 2017-09-27 2019-04-04 北京大学 融合多肽
CN108191979B (zh) * 2017-12-08 2021-01-01 上海晶诺生物科技有限公司 一种荧光互补检测人趋化因子生物学活性的方法
EP3831946A4 (en) * 2018-07-31 2022-08-10 The University of Tokyo METHOD OF MEASUREMENT OF MEMBRANE PROTEIN ACTIVITY
CN110853712B (zh) * 2018-08-01 2022-06-07 清华大学 鉴定多对生物分子间相互作用调控因子的方法
KR102317202B1 (ko) * 2018-10-19 2021-10-25 주식회사 프로티나 유방암 재발 예측 방법
JP2022509850A (ja) * 2018-12-07 2022-01-24 オクタント，インク． タンパク質間相互作用スクリーニングのためのシステム
JP2022513606A (ja) * 2018-12-10 2022-02-09 イエール ユニバーシティ 宿主の生理機能を形成する微生物叢代謝産物
WO2022271264A1 (en) * 2021-06-21 2022-12-29 Massachusetts Institute Of Technology Real-time g-protein coupled receptor (gpcr) linked bioluminescent sensing of biological targets and processes

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2753377B1 (fr) 1996-09-19 1999-09-24 Rhone Merieux Nouvelle association parasiticide a base de 1-n-phenylpyra- zoles et de lactones macrocycliques endectocides
US3737901A (en) 1971-05-03 1973-06-05 Sierra Research Corp Redundant aircraft clock synchronization
JPS57139012A (en) 1981-02-23 1982-08-27 Sankyo Co Ltd Anthelmintic composition
NL8200523A (nl) 1982-02-11 1983-09-01 Univ Leiden Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna.
US4879236A (en) 1984-05-16 1989-11-07 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
US5168062A (en) * 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5168061A (en) * 1986-05-15 1992-12-01 Board Of Regents, The University Of Texas System Human chorionic peptidase-1
US4952500A (en) 1988-02-01 1990-08-28 University Of Georgia Research Foundation, Inc. Cloning systems for Rhodococcus and related bacteria
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5284746A (en) * 1990-02-08 1994-02-08 Zymogenetics, Inc. Methods of producing hybrid G protein-coupled receptors
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
WO1993004169A1 (en) 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
US5439924A (en) 1991-12-23 1995-08-08 Virbac, Inc. Systemic control of parasites
US5702932A (en) 1992-07-20 1997-12-30 University Of Florida Microinjection methods to transform arthropods with exogenous DNA
DE4228457A1 (de) 1992-08-27 1994-04-28 Beiersdorf Ag Herstellung von heterodimerem PDGF-AB mit Hilfe eines bicistronischen Vektorsystems in Säugerzellen
US5814618A (en) * 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5464758A (en) * 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US5656610A (en) 1994-06-21 1997-08-12 University Of Southern California Producing a protein in a mammal by injection of a DNA-sequence into the tongue
FR2722208B1 (fr) 1994-07-05 1996-10-04 Inst Nat Sante Rech Med Nouveau site interne d'entree des ribosomes, vecteur le contenant et utilisation therapeutique
US5871986A (en) 1994-09-23 1999-02-16 The General Hospital Corporation Use of a baculovirus to express and exogenous gene in a mammalian cell
US5736524A (en) 1994-11-14 1998-04-07 Merck & Co.,. Inc. Polynucleotide tuberculosis vaccine
NZ272574A (en) 1995-07-14 1999-02-25 Lilly Eli & Co Nz Ltd Aqueous base suspension concentrate containing at least one ionophore antibiotic and wetting agent and/or a surfactant and xanthan gum optionally with a suspension agent, an antifreeze agent and an antifoaming agent; drench for veterinary treatment
US5780448A (en) 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
AU1283497A (en) * 1995-12-08 1997-06-27 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Method and compositions for monitoring dna binding molecules in living cells
US5773422A (en) 1996-01-29 1998-06-30 Komer; Gene Avermectin formulation
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
EP0959891A1 (en) 1996-07-30 1999-12-01 Ashmont Holdings Limited Anthelmintic formulations
US5945100A (en) 1996-07-31 1999-08-31 Fbp Corporation Tumor delivery vehicles
US5981274A (en) 1996-09-18 1999-11-09 Tyrrell; D. Lorne J. Recombinant hepatitis virus vectors
DE19654079A1 (de) 1996-12-23 1998-06-25 Bayer Ag Endo-ekto-parasitizide Mittel
US6528271B1 (en) * 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
US5891646A (en) * 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
US7128915B2 (en) 1997-09-18 2006-10-31 Research Development Foundation Membrane virus host range mutations and their uses as vaccine substrates
US5994624A (en) 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
TR200001313T2 (tr) * 1997-11-10 2002-06-21 The General Hospital Corporation Protein etkileşimlerini ve fonksiyonel ilişkileri kaydeden tarama sistemleri
US6884870B2 (en) 1998-03-20 2005-04-26 California Institute Of Technology Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells
US6117639A (en) * 1998-08-31 2000-09-12 Vertex Pharmaceuticals Incorporated Fusion proteins, DNA molecules, vectors, and host cells useful for measuring protease activity
US6893827B1 (en) * 2000-02-07 2005-05-17 Applera Corporation Receptor function assay for G-protein coupled receptors and orphan receptors by reporter enzyme mutant complementation
US20030157553A1 (en) 2000-03-03 2003-08-21 Gabriel Berstein Methods of assaying for G protein-coupled receptor ligands and modulators
US6333167B1 (en) * 2000-03-10 2001-12-25 American Home Products Corp. Methods and reagents for identifying inhibitors of proteolysis of membrane-associated proteins
US7018812B2 (en) * 2000-11-03 2006-03-28 Duke University Modified G-protein coupled receptors
EP1205191A1 (en) 2000-11-13 2002-05-15 Centre National De La Recherche Scientifique Targeted modification of intracellular compounds
US6562576B2 (en) * 2001-01-04 2003-05-13 Myriad Genetics, Inc. Yeast two-hybrid system and use thereof
US20030013137A1 (en) * 2001-03-13 2003-01-16 Barak Larry S. Automated methods of detecting receptor activity
EP1421210A4 (en) * 2001-07-31 2005-02-16 Clontech Lab Inc METHOD FOR DETECTING PROTEASE ACTIVITY IN ONE CELL
DE10211063A1 (de) * 2002-03-13 2003-10-09 Axaron Bioscience Ag Neue Verfahren zur Detektion und Analyse von Protein-Interaktionen in vivo
EP1492874B1 (en) * 2002-03-29 2011-01-19 XOMA Technology Ltd. Multigenic vector plasmids and methods for increasing expression of recombinant polypeptides
EP1353182A3 (en) 2002-04-12 2004-02-04 Smithkline Beecham Corporation Method of predicting cell-based assay results using binding profiles
US7001889B2 (en) 2002-06-21 2006-02-21 Merial Limited Anthelmintic oral homogeneous veterinary pastes
KR100890579B1 (ko) * 2002-08-19 2009-04-27 프로테온 주식회사 Ｒｎａ 결합 단백질의 유전자를 융합파트너로 이용한재조합 단백질의 제조방법
DE60325640D1 (de) * 2002-10-25 2009-02-12 Mds Analytical Technologies Us Verfahren zur identifizierung von transmembranrezeptoragonisten mit reduzierter internalisierung
CN1894581B (zh) 2003-07-09 2012-02-01 生命技术公司 检测蛋白-蛋白相互作用的方法

Also Published As

Publication number Publication date
JP2007528713A (ja) 2007-10-18
EP1644734B1 (en) 2011-11-23
WO2005007822A2 (en) 2005-01-27
AU2004257228B8 (en) 2009-01-08
US8017398B2 (en) 2011-09-13
CN1894581A (zh) 2007-01-10
EP1644734A2 (en) 2006-04-12
IL173023A0 (en) 2006-06-11
US20120077706A1 (en) 2012-03-29
US20060147975A1 (en) 2006-07-06
WO2005007822A3 (en) 2005-03-31
AU2004257228B2 (en) 2008-12-18
CA2531698A1 (en) 2005-01-27
ATE534900T1 (de) 2011-12-15
EP2336768A9 (en) 2011-08-10
EP2336768A1 (en) 2011-06-22
CN1894581B (zh) 2012-02-01
AU2004257228A1 (en) 2005-01-27
US20050100934A1 (en) 2005-05-12
EP1644734A4 (en) 2007-04-25
US7049076B2 (en) 2006-05-23

Similar Documents

Publication Publication Date Title
JP4734498B2 (ja) タンパク質−タンパク質相互作用を解析する方法
CN102187225B (zh) 使用蛋白酶活化的受体鉴别调节蛋白质-蛋白质相互作用的分子
EP2002021B1 (en) Methods for assaying protein-protein interaction
WO2007149807A1 (en) Methods for identifying modifiers of gpr1 activity
US20070224615A1 (en) Methods for assaying protein-protein interactions
AU2013229528A1 (en) Membrane span-kinase fusion protein and the uses thereof
WO2007032793A1 (en) Multiplex array useful for assaying protein-protein interaction
HK1162664B (en) Identifying molecules that modulate protein- protein interactions using protease activated reporters
WO2007002641A2 (en) Method for identifying cart receptor and uses thereof

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20071018

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100422

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20100722

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20100729

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100810

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100930

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20101227

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110127

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20110228

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20110228

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110316

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

Ref document number: 4734498

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140513

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term