JP4621197B2 - 非標準塩基を用いた核酸増幅 - Google Patents

非標準塩基を用いた核酸増幅 Download PDF

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JP4621197B2
JP4621197B2 JP2006501040A JP2006501040A JP4621197B2 JP 4621197 B2 JP4621197 B2 JP 4621197B2 JP 2006501040 A JP2006501040 A JP 2006501040A JP 2006501040 A JP2006501040 A JP 2006501040A JP 4621197 B2 JP4621197 B2 JP 4621197B2
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nucleic acid
standard
acid amplification
bases
reaction
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JP2006515760A5 (enExample
JP2006515760A (ja
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プルーデント，ジェームズ，アール．
ジョンソン，スコット，シー．
モーザー，マイケル，ジェイ．
マーシャル，デイヴィッド，ジェイ．
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エラジェン バイオサイエンシズ インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Steroid Compounds (AREA)
JP2006501040A 2003-01-17 2004-01-17 非標準塩基を用いた核酸増幅 Expired - Fee Related JP4621197B2 (ja)

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Application Number Priority Date Filing Date Title
US44092103P 2003-01-17 2003-01-17
PCT/US2004/001397 WO2004065550A2 (en) 2003-01-17 2004-01-17 Nucleic acid amplification using non-standard bases

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JP2006515760A JP2006515760A (ja) 2006-06-08
JP2006515760A5 JP2006515760A5 (enExample) 2007-03-08
JP4621197B2 true JP4621197B2 (ja) 2011-01-26

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JP2006501040A Expired - Fee Related JP4621197B2 (ja) 2003-01-17 2004-01-17 非標準塩基を用いた核酸増幅

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US (1) US7514212B2 (enExample)
EP (1) EP1590482B1 (enExample)
JP (1) JP4621197B2 (enExample)
AT (1) ATE417129T1 (enExample)
AU (1) AU2004206246B2 (enExample)
CA (1) CA2513626C (enExample)
DE (1) DE602004018270D1 (enExample)
WO (1) WO2004065550A2 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7498136B2 (en) 2005-03-18 2009-03-03 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of Neisseria
CA2610863C (en) * 2005-06-07 2017-01-17 Eragen Biosciences, Inc. Methods for detection and typing of nucleic acids
US7759062B2 (en) 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
WO2009123216A1 (ja) 2008-03-31 2009-10-08 独立行政法人理化学研究所 高選択・高効率でｐｃｒ増幅が可能な新規ｄｎａ
US10865431B1 (en) * 2008-06-17 2020-12-15 Steven A Benner Polymerase incorporation of non-standard nucleotides
JP5715068B2 (ja) 2009-01-30 2015-05-07 マイクロニクス， インコーポレイテッド 携帯型高利得蛍光検出システム
CN102639712A (zh) * 2009-10-23 2012-08-15 卢米耐克斯公司 用于提高反应特异性的具有非标准碱基的扩增引物
CN102740976B (zh) 2010-01-29 2016-04-20 精密公司 取样-应答微流体盒
US10752944B2 (en) 2011-09-06 2020-08-25 Gen-Probe Incorporated Circularized templates for sequencing
EP3620533B1 (en) 2011-09-06 2023-01-18 Gen-Probe Incorporated Closed nucleic acid structures
US20150259571A1 (en) * 2012-08-31 2015-09-17 Empire Technology Development Llc Lignin membranes and coatings
EP2934751B1 (en) 2012-12-21 2019-05-29 Micronics, Inc. Low elasticity films for microfluidic use
KR102102123B1 (ko) 2012-12-21 2020-04-20 퍼킨엘머 헬스 사이언시즈, 아이엔씨. 유체 공학 회로 및 관련 제조 방법
EP2935559B1 (en) 2012-12-21 2020-09-16 PerkinElmer Health Sciences, Inc. Fluorescence detection system
JP6484222B2 (ja) 2013-05-07 2019-03-13 マイクロニクス， インコーポレイテッド 核酸の調製および分析のためのデバイス
EP2994750B1 (en) 2013-05-07 2020-08-12 PerkinElmer Health Sciences, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
CN105189750B (zh) 2013-05-07 2020-07-28 珀金埃尔默健康科学有限公司 使用粘土矿物和碱性溶液制备含核酸样品的方法
AU2016257426B2 (en) 2015-05-01 2020-01-16 Gen-Probe Incorporated Multiplex invasive cleavage assays
WO2023043280A1 (ko) * 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US5470974A (en) * 1985-03-15 1995-11-28 Neu-Gene Development Group Uncharged polynucleotide-binding polymers
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US6054270A (en) * 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US6444798B1 (en) * 1988-06-06 2002-09-03 Steven Albert Benner Chimeras of sulfur-linked oligonucleotide analogs and DNA and RNA
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
GB8902689D0 (en) 1989-02-07 1989-03-30 Ici Plc Assay method
US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
GB8920097D0 (en) 1989-09-06 1989-10-18 Ici Plc Amplification processes
US6007984A (en) * 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5412088A (en) * 1989-11-20 1995-05-02 Rutgers, The State University 6-O-substituted guanosine derivatives
US5177064A (en) * 1990-07-13 1993-01-05 University Of Florida Targeted drug delivery via phosphonate derivatives
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6140496A (en) * 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US5432272A (en) * 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5965364A (en) * 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US6037120A (en) * 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
ES2101083T3 (es) * 1990-12-21 1997-07-01 Hoffmann La Roche Tipado del dna hla dqbeta.
DE4129653A1 (de) * 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5958784A (en) * 1992-03-25 1999-09-28 Benner; Steven Albert Predicting folded structures of proteins
AU3933393A (en) 1993-03-17 1994-10-11 City Of Hope Nucleic acid sequences having single stranded ends
US5593836A (en) * 1993-05-14 1997-01-14 Niemiec; John T. Primers and probes for detecting Pneumocystis carinii
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
WO1995021271A1 (en) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5763169A (en) * 1995-01-13 1998-06-09 Chiron Diagnostics Corporation Nucleic acid probes for the detection and identification of fungi
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5736330A (en) * 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5705621A (en) * 1995-11-17 1998-01-06 Isis Pharmaceuticals, Inc. Oligomeric phosphite, phosphodiester, Phosphorothioate and phosphorodithioate compounds and intermediates for preparing same
AU2320597A (en) * 1996-03-19 1997-10-10 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US5780233A (en) 1996-06-06 1998-07-14 Wisconsin Alumni Research Foundation Artificial mismatch hybridization
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
JP4185185B2 (ja) 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖ｄｎａを利用したｄｎａの検出方法
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
JP4471494B2 (ja) * 1998-05-14 2010-06-02 ルミネックス コーポレイション ダイオードレーザをベースとした測定装置
WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
WO2001051669A2 (en) * 2000-01-15 2001-07-19 Genelabs Technologies, Inc. Nucleic acid binding assay and selection method
AU7125401A (en) * 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
DE60137649D1 (de) * 2000-10-14 2009-03-26 Eragen Biosciences Inc Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen
US7374882B2 (en) * 2000-11-28 2008-05-20 Riken Method for base sequencing and biologically active nucleic acids
US20030194705A1 (en) * 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding

Also Published As

Publication number Publication date
ATE417129T1 (de) 2008-12-15
EP1590482A2 (en) 2005-11-02
US20070105099A1 (en) 2007-05-10
WO2004065550A2 (en) 2004-08-05
WO2004065550A3 (en) 2004-12-23
US7514212B2 (en) 2009-04-07
AU2004206246A1 (en) 2004-08-05
JP2006515760A (ja) 2006-06-08
AU2004206246B2 (en) 2009-10-01
CA2513626C (en) 2013-03-19
EP1590482A4 (en) 2006-07-26
EP1590482B1 (en) 2008-12-10
DE602004018270D1 (de) 2009-01-22
CA2513626A1 (en) 2004-08-05

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