DE602004018270D1 - Nukleinsäureamplifikation mit nichtstandardbasen - Google Patents
Nukleinsäureamplifikation mit nichtstandardbasenInfo
- Publication number
- DE602004018270D1 DE602004018270D1 DE602004018270T DE602004018270T DE602004018270D1 DE 602004018270 D1 DE602004018270 D1 DE 602004018270D1 DE 602004018270 T DE602004018270 T DE 602004018270T DE 602004018270 T DE602004018270 T DE 602004018270T DE 602004018270 D1 DE602004018270 D1 DE 602004018270D1
- Authority
- DE
- Germany
- Prior art keywords
- amplification
- standard
- nucleic acid
- standard bases
- acid amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003321 amplification Effects 0.000 title abstract 7
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 7
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 238000006243 chemical reaction Methods 0.000 abstract 3
- 239000002777 nucleoside Substances 0.000 abstract 2
- -1 nucleoside triphosphates Chemical class 0.000 abstract 2
- 239000001226 triphosphate Substances 0.000 abstract 2
- 235000011178 triphosphate Nutrition 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US44092103P | 2003-01-17 | 2003-01-17 | |
PCT/US2004/001397 WO2004065550A2 (en) | 2003-01-17 | 2004-01-17 | Nucleic acid amplification using non-standard bases |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602004018270D1 true DE602004018270D1 (de) | 2009-01-22 |
Family
ID=32771878
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602004018270T Expired - Lifetime DE602004018270D1 (de) | 2003-01-17 | 2004-01-17 | Nukleinsäureamplifikation mit nichtstandardbasen |
Country Status (8)
Country | Link |
---|---|
US (1) | US7514212B2 (de) |
EP (1) | EP1590482B1 (de) |
JP (1) | JP4621197B2 (de) |
AT (1) | ATE417129T1 (de) |
AU (1) | AU2004206246B2 (de) |
CA (1) | CA2513626C (de) |
DE (1) | DE602004018270D1 (de) |
WO (1) | WO2004065550A2 (de) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7498136B2 (en) | 2005-03-18 | 2009-03-03 | Eragen Biosciences, Inc. | Methods for detecting multiple species and subspecies of Neisseria |
EP2489746B1 (de) | 2005-06-07 | 2016-02-03 | Luminex Corporation | Verfahren zur Detektion und Typisierung von Nucleinsäuren |
US7759062B2 (en) | 2006-06-09 | 2010-07-20 | Third Wave Technologies, Inc. | T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection |
CN101981186A (zh) | 2008-03-31 | 2011-02-23 | 塔古西库斯生物株式会社 | 可高选择性且高效率地进行pcr扩增的新型dna |
US10865431B1 (en) * | 2008-06-17 | 2020-12-15 | Steven A Benner | Polymerase incorporation of non-standard nucleotides |
EP2391883B1 (de) | 2009-01-30 | 2018-03-07 | Micronics, Inc. | Tragbares hochleistungsfluoreszenznachweissystem |
US8440406B2 (en) * | 2009-10-23 | 2013-05-14 | Luminex Corporation | Amplification primers with non-standard bases for increased reaction specificity |
CN102740976B (zh) | 2010-01-29 | 2016-04-20 | 精密公司 | 取样-应答微流体盒 |
US10767208B2 (en) | 2011-09-06 | 2020-09-08 | Gen-Probe Incorporated | Closed nucleic acid structures |
EP2753714B1 (de) | 2011-09-06 | 2017-04-12 | Gen-Probe Incorporated | Zirkularisierte schablonen zur sequenzierung |
US20150259571A1 (en) * | 2012-08-31 | 2015-09-17 | Empire Technology Development Llc | Lignin membranes and coatings |
JP6498125B2 (ja) | 2012-12-21 | 2019-04-10 | マイクロニクス, インコーポレイテッド | 流体回路および関連する製造方法 |
US20150346097A1 (en) | 2012-12-21 | 2015-12-03 | Micronics, Inc. | Portable fluorescence detection system and microassay cartridge |
JP6935167B2 (ja) | 2012-12-21 | 2021-09-15 | ペルキネルマー ヘルス サイエンシーズ, インコーポレイテッド | マイクロ流体使用のための低弾性フィルム |
CN105189750B (zh) | 2013-05-07 | 2020-07-28 | 珀金埃尔默健康科学有限公司 | 使用粘土矿物和碱性溶液制备含核酸样品的方法 |
WO2014182844A1 (en) | 2013-05-07 | 2014-11-13 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
AU2014262726B2 (en) | 2013-05-07 | 2019-09-19 | Perkinelmer Health Sciences, Inc. | Device for preparation and analysis of nucleic acids |
JP6688318B2 (ja) | 2015-05-01 | 2020-04-28 | ジェン−プローブ・インコーポレーテッド | 多重侵入切断アッセイ |
WO2023043280A1 (ko) * | 2021-09-17 | 2023-03-23 | 주식회사 씨젠 | 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출 |
Family Cites Families (48)
Publication number | Priority date | Publication date | Assignee | Title |
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US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
US5470974A (en) | 1985-03-15 | 1995-11-28 | Neu-Gene Development Group | Uncharged polynucleotide-binding polymers |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4996143A (en) | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US6054270A (en) | 1988-05-03 | 2000-04-25 | Oxford Gene Technology Limited | Analying polynucleotide sequences |
US6444798B1 (en) | 1988-06-06 | 2002-09-03 | Steven Albert Benner | Chimeras of sulfur-linked oligonucleotide analogs and DNA and RNA |
GB8827160D0 (en) | 1988-11-21 | 1988-12-29 | Apothekernes Lab | Detection & quantitative determination of rna & dna |
GB8902689D0 (en) | 1989-02-07 | 1989-03-30 | Ici Plc | Assay method |
US5856092A (en) | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
GB8920097D0 (en) | 1989-09-06 | 1989-10-18 | Ici Plc | Amplification processes |
US6007984A (en) | 1989-11-01 | 1999-12-28 | Zeneca Limited | Detection of DNA/RNA by fluorescence polarization |
US5412088A (en) | 1989-11-20 | 1995-05-02 | Rutgers, The State University | 6-O-substituted guanosine derivatives |
US5177064A (en) | 1990-07-13 | 1993-01-05 | University Of Florida | Targeted drug delivery via phosphonate derivatives |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
US6037120A (en) | 1995-10-12 | 2000-03-14 | Benner; Steven Albert | Recognition of oligonucleotides containing non-standard base pairs |
US5965364A (en) | 1990-10-09 | 1999-10-12 | Benner; Steven Albert | Method for selecting functional deoxyribonucleotide derivatives |
US6140496A (en) | 1990-10-09 | 2000-10-31 | Benner; Steven Albert | Precursors for deoxyribonucleotides containing non-standard nucleosides |
ES2101083T3 (es) | 1990-12-21 | 1997-07-01 | Hoffmann La Roche | Tipado del dna hla dqbeta. |
DE4129653A1 (de) | 1991-09-06 | 1993-03-11 | Boehringer Mannheim Gmbh | Verfahren zum nachweis aehnlicher nukleinsaeuren |
US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
US5958784A (en) | 1992-03-25 | 1999-09-28 | Benner; Steven Albert | Predicting folded structures of proteins |
WO1994021820A1 (en) | 1993-03-17 | 1994-09-29 | City Of Hope | Nucleic acid sequences having single stranded ends |
US5593836A (en) * | 1993-05-14 | 1997-01-14 | Niemiec; John T. | Primers and probes for detecting Pneumocystis carinii |
US6156501A (en) | 1993-10-26 | 2000-12-05 | Affymetrix, Inc. | Arrays of modified nucleic acid probes and methods of use |
DE69531542T2 (de) | 1994-02-07 | 2004-06-24 | Beckman Coulter, Inc., Fullerton | Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse |
US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
US5763169A (en) * | 1995-01-13 | 1998-06-09 | Chiron Diagnostics Corporation | Nucleic acid probes for the detection and identification of fungi |
GB9507238D0 (en) | 1995-04-07 | 1995-05-31 | Isis Innovation | Detecting dna sequence variations |
US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
US5705621A (en) * | 1995-11-17 | 1998-01-06 | Isis Pharmaceuticals, Inc. | Oligomeric phosphite, phosphodiester, Phosphorothioate and phosphorodithioate compounds and intermediates for preparing same |
WO1997035033A1 (en) | 1996-03-19 | 1997-09-25 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
US5780233A (en) | 1996-06-06 | 1998-07-14 | Wisconsin Alumni Research Foundation | Artificial mismatch hybridization |
US6124092A (en) | 1996-10-04 | 2000-09-26 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
JP4185185B2 (ja) | 1997-05-08 | 2008-11-26 | 征夫 軽部 | 部分二重鎖dnaを利用したdnaの検出方法 |
US5928869A (en) | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
GB9725197D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
JP4471494B2 (ja) | 1998-05-14 | 2010-06-02 | ルミネックス コーポレイション | ダイオードレーザをベースとした測定装置 |
EP2045334A1 (de) | 1998-06-24 | 2009-04-08 | Illumina, Inc. | Dekodierung von Arraysensoren mit Mikrosphären |
US6528254B1 (en) | 1999-10-29 | 2003-03-04 | Stratagene | Methods for detection of a target nucleic acid sequence |
AU2001229576A1 (en) | 2000-01-15 | 2001-07-24 | Genelabs Technologies, Inc. | Nucleic acid binding site assay and selection method |
JP5138141B2 (ja) * | 2000-05-19 | 2013-02-06 | ルミネックス コーポレーション | 核酸の検出用物質及び検出方法 |
ATE422557T1 (de) | 2000-10-14 | 2009-02-15 | Eragen Biosciences Inc | Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen |
US7374882B2 (en) * | 2000-11-28 | 2008-05-20 | Riken | Method for base sequencing and biologically active nucleic acids |
US20030194705A1 (en) | 2002-03-01 | 2003-10-16 | Schroth Gary P. | Methods of using unnatural nucleobases for decoding |
-
2004
- 2004-01-17 WO PCT/US2004/001397 patent/WO2004065550A2/en active Application Filing
- 2004-01-17 US US10/542,464 patent/US7514212B2/en active Active
- 2004-01-17 EP EP04703107A patent/EP1590482B1/de not_active Expired - Lifetime
- 2004-01-17 JP JP2006501040A patent/JP4621197B2/ja not_active Expired - Lifetime
- 2004-01-17 DE DE602004018270T patent/DE602004018270D1/de not_active Expired - Lifetime
- 2004-01-17 AU AU2004206246A patent/AU2004206246B2/en not_active Expired
- 2004-01-17 AT AT04703107T patent/ATE417129T1/de not_active IP Right Cessation
- 2004-01-17 CA CA2513626A patent/CA2513626C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP4621197B2 (ja) | 2011-01-26 |
ATE417129T1 (de) | 2008-12-15 |
AU2004206246A1 (en) | 2004-08-05 |
WO2004065550A2 (en) | 2004-08-05 |
EP1590482A2 (de) | 2005-11-02 |
EP1590482B1 (de) | 2008-12-10 |
US20070105099A1 (en) | 2007-05-10 |
CA2513626A1 (en) | 2004-08-05 |
US7514212B2 (en) | 2009-04-07 |
JP2006515760A (ja) | 2006-06-08 |
EP1590482A4 (de) | 2006-07-26 |
AU2004206246B2 (en) | 2009-10-01 |
CA2513626C (en) | 2013-03-19 |
WO2004065550A3 (en) | 2004-12-23 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |