JP4500547B2 - センサーの反応性ゾーンに固定された分子プローブの検出 - Google Patents
センサーの反応性ゾーンに固定された分子プローブの検出 Download PDFInfo
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- JP4500547B2 JP4500547B2 JP2003562625A JP2003562625A JP4500547B2 JP 4500547 B2 JP4500547 B2 JP 4500547B2 JP 2003562625 A JP2003562625 A JP 2003562625A JP 2003562625 A JP2003562625 A JP 2003562625A JP 4500547 B2 JP4500547 B2 JP 4500547B2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
- C12Q1/003—Functionalisation
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Measurement Of Length, Angles, Or The Like Using Electric Or Magnetic Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR02/00676 | 2002-01-21 | ||
| FR0200676A FR2835058B1 (fr) | 2002-01-21 | 2002-01-21 | Procede de detection d'au moins un parametre caracteristique de molecules sondes fixees sur au moins une zone active d'un capteur |
| PCT/FR2002/004283 WO2003062811A1 (fr) | 2002-01-21 | 2002-12-11 | Detection de molecules sondes fixees sur une zone active d'un capteur. |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2005515475A JP2005515475A (ja) | 2005-05-26 |
| JP2005515475A6 JP2005515475A6 (ja) | 2005-08-04 |
| JP2005515475A5 JP2005515475A5 (enExample) | 2006-01-19 |
| JP4500547B2 true JP4500547B2 (ja) | 2010-07-14 |
Family
ID=27589525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003562625A Expired - Lifetime JP4500547B2 (ja) | 2002-01-21 | 2002-12-11 | センサーの反応性ゾーンに固定された分子プローブの検出 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7993825B2 (enExample) |
| EP (1) | EP1468277B1 (enExample) |
| JP (1) | JP4500547B2 (enExample) |
| AT (1) | ATE366321T1 (enExample) |
| DE (1) | DE60221046T2 (enExample) |
| ES (1) | ES2289176T3 (enExample) |
| FR (1) | FR2835058B1 (enExample) |
| WO (1) | WO2003062811A1 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7317216B2 (en) * | 2003-10-31 | 2008-01-08 | University Of Hawaii | Ultrasensitive biochemical sensing platform |
| US20080009002A1 (en) * | 2004-11-09 | 2008-01-10 | The Regents Of The University Of California | Analyte Identification Using Electronic Devices |
| KR100773550B1 (ko) * | 2006-04-03 | 2007-11-07 | 삼성전자주식회사 | 생분자의 고정 없이 전계 효과 트랜지스터를 이용하여생분자를 검출하는 방법 |
| KR100773549B1 (ko) | 2006-04-03 | 2007-11-07 | 삼성전자주식회사 | 동일 전계 효과 트랜지스터를 이용하여 생분자를 검출하는방법 |
| KR101195612B1 (ko) * | 2006-04-10 | 2012-10-29 | 삼성전자주식회사 | 금층을 포함하는 전계 효과 트랜지스터, 상기 전계 효과 트랜지스터를 포함하는 미세유동장치, 및 상기 전계 효과 트랜지스터 및 미세유동장치를 이용하여 티올기를 포함하는 분석물을 검출하는 방법 |
| US8262900B2 (en) * | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US11339430B2 (en) | 2007-07-10 | 2022-05-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| WO2008076406A2 (en) | 2006-12-14 | 2008-06-26 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
| US8198658B2 (en) * | 2007-06-13 | 2012-06-12 | Samsung Electronics Co., Ltd. | Device and method for detecting biomolecules using adsorptive medium and field effect transistor |
| KR101375547B1 (ko) * | 2007-06-19 | 2014-03-25 | 삼성전자주식회사 | 표준 전극이 포함된 전계 효과 트랜지스터 어레이를 이용한생체 분자의 검출 장치 및 방법 |
| US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US8776573B2 (en) | 2009-05-29 | 2014-07-15 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| US20120261274A1 (en) | 2009-05-29 | 2012-10-18 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| US8415176B2 (en) | 2010-06-30 | 2013-04-09 | Life Technologies Corporation | One-transistor pixel array |
| CN106932456B (zh) * | 2010-06-30 | 2020-02-21 | 生命科技公司 | 用于测试isfet阵列的方法和装置 |
| CN103154718B (zh) | 2010-06-30 | 2015-09-23 | 生命科技公司 | 感测离子的电荷堆积电路和方法 |
| US11307166B2 (en) | 2010-07-01 | 2022-04-19 | Life Technologies Corporation | Column ADC |
| US8653567B2 (en) | 2010-07-03 | 2014-02-18 | Life Technologies Corporation | Chemically sensitive sensor with lightly doped drains |
| WO2012036679A1 (en) | 2010-09-15 | 2012-03-22 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
| US9970984B2 (en) | 2011-12-01 | 2018-05-15 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
| US8786331B2 (en) | 2012-05-29 | 2014-07-22 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
| US9080968B2 (en) | 2013-01-04 | 2015-07-14 | Life Technologies Corporation | Methods and systems for point of use removal of sacrificial material |
| US9841398B2 (en) | 2013-01-08 | 2017-12-12 | Life Technologies Corporation | Methods for manufacturing well structures for low-noise chemical sensors |
| US8963216B2 (en) | 2013-03-13 | 2015-02-24 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
| US9835585B2 (en) | 2013-03-15 | 2017-12-05 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
| CN105051525B (zh) | 2013-03-15 | 2019-07-26 | 生命科技公司 | 具有薄导电元件的化学设备 |
| EP2972280B1 (en) | 2013-03-15 | 2021-09-29 | Life Technologies Corporation | Chemical sensor with consistent sensor surface areas |
| US20140336063A1 (en) | 2013-05-09 | 2014-11-13 | Life Technologies Corporation | Windowed Sequencing |
| US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
| US10077472B2 (en) | 2014-12-18 | 2018-09-18 | Life Technologies Corporation | High data rate integrated circuit with power management |
| EP4354131A3 (en) | 2014-12-18 | 2024-06-26 | Life Technologies Corporation | High data rate integrated circuit with transmitter configuration |
| KR20170097712A (ko) | 2014-12-18 | 2017-08-28 | 라이프 테크놀로지스 코포레이션 | 대형 fet 어레이를 사용한 분석물 측정을 위한 방법과 장치 |
| US11293897B2 (en) * | 2018-11-30 | 2022-04-05 | Taiwan Semiconductor Manufacturing Company, Ltd. | High sensitivity ISFET sensor |
| GB202017861D0 (en) | 2020-11-12 | 2020-12-30 | Ulifetec Sas | Method |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4238757A (en) * | 1976-03-19 | 1980-12-09 | General Electric Company | Field effect transistor for detection of biological reactions |
| EP0213825A3 (en) * | 1985-08-22 | 1989-04-26 | Molecular Devices Corporation | Multiple chemically modulated capacitance |
| US5200051A (en) * | 1988-11-14 | 1993-04-06 | I-Stat Corporation | Wholly microfabricated biosensors and process for the manufacture and use thereof |
| US5242793A (en) * | 1989-03-08 | 1993-09-07 | Kanzaki Paper Manufacturing Co., Ltd. | Selective permeable membrane and electrode using the same |
| US5498521A (en) * | 1990-01-24 | 1996-03-12 | President And Fellows Of Harvard College | Diagnosis of hereditary retinal degenerative diseases |
| CA2121797A1 (en) * | 1991-10-21 | 1993-04-29 | James W. Holm-Kennedy | Method and device for biochemical sensing |
| US5846708A (en) * | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
| WO1993022456A1 (en) * | 1992-04-27 | 1993-11-11 | Trustees Of Dartmouth College | Detection of gene sequences in biological fluids |
| US6331274B1 (en) * | 1993-11-01 | 2001-12-18 | Nanogen, Inc. | Advanced active circuits and devices for molecular biological analysis and diagnostics |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US5805014A (en) * | 1996-03-01 | 1998-09-08 | Compaq Computer Corporation | System having active pull-down circuit and method |
| WO1997039145A1 (en) * | 1996-04-17 | 1997-10-23 | Motorola Inc. | Transistor-based molecular detection apparatus and method |
| US5827482A (en) * | 1996-08-20 | 1998-10-27 | Motorola Corporation | Transistor-based apparatus and method for molecular detection and field enhancement |
| US6322963B1 (en) * | 1998-06-15 | 2001-11-27 | Biosensor Systems Design., Inc. | Sensor for analyte detection |
| US6281006B1 (en) * | 1998-08-24 | 2001-08-28 | Therasense, Inc. | Electrochemical affinity assay |
| US6875619B2 (en) * | 1999-11-12 | 2005-04-05 | Motorola, Inc. | Microfluidic devices comprising biochannels |
| FR2805826B1 (fr) * | 2000-03-01 | 2002-09-20 | Nucleica | Nouvelles puces a adn |
| US6489160B2 (en) * | 2000-03-16 | 2002-12-03 | Kabushiki Kaisha Toshiba | Method for producing nucleic acid strand immobilized carrier |
| EP1423687A1 (en) * | 2001-08-08 | 2004-06-02 | The Arizona Board of Regents | Nucleic acid field effect transistor |
| EP1460130B1 (en) * | 2001-12-19 | 2007-03-21 | Hitachi High-Technologies Corporation | Potentiometric dna microarray, process for producing the same and method of analyzing nucleic acid |
| EP1570091B1 (fr) * | 2002-12-11 | 2012-02-08 | Centre National De La Recherche Scientifique (Cnrs) | Procede de detection electronique d'au moins une interaction specifique entre des molecules sondes et des biomolecules cibles. |
-
2002
- 2002-01-21 FR FR0200676A patent/FR2835058B1/fr not_active Expired - Fee Related
- 2002-12-11 EP EP02799807A patent/EP1468277B1/fr not_active Expired - Lifetime
- 2002-12-11 JP JP2003562625A patent/JP4500547B2/ja not_active Expired - Lifetime
- 2002-12-11 DE DE60221046T patent/DE60221046T2/de not_active Expired - Lifetime
- 2002-12-11 WO PCT/FR2002/004283 patent/WO2003062811A1/fr not_active Ceased
- 2002-12-11 ES ES02799807T patent/ES2289176T3/es not_active Expired - Lifetime
- 2002-12-11 AT AT02799807T patent/ATE366321T1/de not_active IP Right Cessation
- 2002-12-11 US US10/501,772 patent/US7993825B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20060011911A1 (en) | 2006-01-19 |
| DE60221046T2 (de) | 2008-03-06 |
| FR2835058B1 (fr) | 2004-03-12 |
| ATE366321T1 (de) | 2007-07-15 |
| EP1468277B1 (fr) | 2007-07-04 |
| DE60221046D1 (de) | 2007-08-16 |
| US7993825B2 (en) | 2011-08-09 |
| ES2289176T3 (es) | 2008-02-01 |
| FR2835058A1 (fr) | 2003-07-25 |
| EP1468277A1 (fr) | 2004-10-20 |
| JP2005515475A (ja) | 2005-05-26 |
| WO2003062811A1 (fr) | 2003-07-31 |
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