JP4857820B2 - Dnaセンシング方法 - Google Patents
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- JP4857820B2 JP4857820B2 JP2006057706A JP2006057706A JP4857820B2 JP 4857820 B2 JP4857820 B2 JP 4857820B2 JP 2006057706 A JP2006057706 A JP 2006057706A JP 2006057706 A JP2006057706 A JP 2006057706A JP 4857820 B2 JP4857820 B2 JP 4857820B2
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
- G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
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Description
[1] 半導体上に反応ゲート絶縁部としてシリコン酸化物を含む厚さ10〜50nmの第1の絶縁層が形成された電界効果型トランジスタの上記第1の絶縁層の上に、アミノ系官能基、カルボキシル系官能基又はメルカプト系官能基を有する炭素数3〜20の直鎖状炭化水素基を有するアルコキシシランで構成された厚さ3nm以下の第1の有機単分子膜を形成し、該第1の有機単分子膜に塩基数3〜35の短鎖プローブDNAを上記反応性官能基を介して、直接又は架橋分子を介して結合させ、上記プローブDNAに対し、該プローブDNAの完全相補配列を有するDNA、該完全相補配列に対して1個の塩基が異なるミスマッチ配列を有するDNA、該完全相補配列に対して2個の塩基が異なるミスマッチ配列を有するDNA又は該完全相補配列に対して3個の塩基が異なるミスマッチ配列を有するDNAであるターゲットDNAをハイブリダイゼーション反応させて、該反応によるプローブDNAの負電荷の変化により生じる絶縁層の表面電位変化を検出することを特徴とするDNAセンシング方法。
本発明の半導体DNAセンシングデバイスは、半導体上に反応ゲート絶縁部としてシリコン酸化物又は無機酸化物を含む第1の絶縁層が形成された電界効果型トランジスタの上記第1の絶縁層の上に、反応性官能基を有する有機分子で構成された第1の有機単分子膜を形成し、第1の有機単分子膜に塩基数3〜35の短鎖プローブDNAを上記反応性官能基を介して、直接又は架橋分子を介して結合させてなる、プローブDNA/有機単分子膜/絶縁層/半導体構造を検出部として備えるものである。
プローブDNAの固定が可能なアミノ系単分子膜3bと、DNAに対して非反応性のフッ化アルキル単分子膜3aとがパターン形成された図4に示されるような基板を用いてDNAの位置選択的な固定化の確認を行った。なお、図4中、1はシリコン基板、2は絶縁層である。
実験例1における予備検討を踏まえ、上述のデバイスを用いて完全相補配列を有するDNAのハイブリダイゼーションの検出を行った。
上述のデバイスを用いた混合塩基を含む完全相補配列を有するDNAのハイブリダイゼーションの検出を行った。ターゲットDNAには3’末端にアミノ基が修飾された3'-NH2-ACGAACATAGCCCGCCTTAC-5'(配列番号5)を、プローブDNAには完全相補の5'-TGCTGTTATCGGGCGGAATG-3'(配列番号6)を用い、実施例1と同様の方法で電圧応答の測定を実施したところ、混合塩基DNAの場合も約50mV程度の正方向への電圧シフトが観測された。この結果は、混合塩基からなる実際のDNAにおいても、本発明のデバイスによるセンシングが可能であることを示している。
ミスマッチ配列を含むターゲットDNAを用いて、ミスマッチ検出を行った。プローブDNAは実施例2と同じであるが、ターゲットDNAとして1,3又は5個の塩基のミスマッチを含むもの(一塩基多型)を用い、1塩基ミスマッチDNAとして5'-TGCTTGTATCGTGCGGAATG-3'(配列番号7)、3塩基ミスマッチDNAとして5'-TGCTAGTATCGTGCGGAGTG-3'(配列番号8)、5塩基ミスマッチDNAとして5'-AGCTAGTATCGTGCCGAGTG-3'(配列番号9)を用いた。
2 絶縁層
3 有機単分子膜
4 ゲート電極
5 ソース電極
6 ドレイン電極
7 チャンネル領域
8 参照部
9 検出部
10 テンプレート部
11 プローブDNA
12 架橋分子
13 ターゲットDNA
配列番号2:Designed oligonucleotide. 3'- end is terminated by NH2 group.
配列番号3:Designed oligonucleotide.
配列番号4:Designed oligonucleotide.
配列番号5:Designed oligonucleotide. 3'- end is terminated by NH2 group.
配列番号6:Designed oligonucleotide.
配列番号7:Designed oligonucleotide.
配列番号8:Designed oligonucleotide.
配列番号9:Designed oligonucleotide.
Claims (1)
- 半導体上に反応ゲート絶縁部としてシリコン酸化物を含む厚さ10〜50nmの第1の絶縁層が形成された電界効果型トランジスタの上記第1の絶縁層の上に、アミノ系官能基、カルボキシル系官能基又はメルカプト系官能基を有する炭素数3〜20の直鎖状炭化水素基を有するアルコキシシランで構成された厚さ3nm以下の第1の有機単分子膜を形成し、該第1の有機単分子膜に塩基数3〜35の短鎖プローブDNAを上記反応性官能基を介して、直接又は架橋分子を介して結合させ、上記プローブDNAに対し、該プローブDNAの完全相補配列を有するDNA、該完全相補配列に対して1個の塩基が異なるミスマッチ配列を有するDNA、該完全相補配列に対して2個の塩基が異なるミスマッチ配列を有するDNA又は該完全相補配列に対して3個の塩基が異なるミスマッチ配列を有するDNAであるターゲットDNAをハイブリダイゼーション反応させて、該反応によるプローブDNAの負電荷の変化により生じる絶縁層の表面電位変化を検出することを特徴とするDNAセンシング方法。
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JP2006057706A JP4857820B2 (ja) | 2006-03-03 | 2006-03-03 | Dnaセンシング方法 |
US11/514,843 US20070207471A1 (en) | 2006-03-03 | 2006-09-05 | Semiconductor DNA sensing device and DNA sensing method |
US12/717,074 US20100221841A1 (en) | 2006-03-03 | 2010-03-03 | Semiconductor dna sensing device and dna sensing method |
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JP2006057706A JP4857820B2 (ja) | 2006-03-03 | 2006-03-03 | Dnaセンシング方法 |
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JP4857820B2 true JP4857820B2 (ja) | 2012-01-18 |
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JP2009156827A (ja) * | 2007-12-28 | 2009-07-16 | Univ Waseda | 半導体センシング用電界効果型トランジスタ及びこれを用いた半導体センシングデバイス |
WO2009119319A1 (ja) * | 2008-03-27 | 2009-10-01 | 株式会社堀場製作所 | イオンセンサ |
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CN109234158B (zh) | 2018-10-08 | 2021-08-06 | 北京京东方技术开发有限公司 | 生物芯片及其制造方法、操作方法、生物检测系统 |
CN110556305A (zh) * | 2019-08-09 | 2019-12-10 | 复旦大学 | 一种基于拓展栅晶体管结构的表面电位测量方法 |
JP2021032607A (ja) * | 2019-08-20 | 2021-03-01 | 学校法人早稲田大学 | アプタマー固定化半導体センシングデバイス及び非荷電分子の検出方法 |
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TW247368B (en) * | 1993-09-29 | 1995-05-11 | Seiko Epuson Co | Current regulating semiconductor integrate circuit device and fabrication method of the same |
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US6248539B1 (en) * | 1997-09-05 | 2001-06-19 | The Scripps Research Institute | Porous semiconductor-based optical interferometric sensor |
US6287776B1 (en) * | 1998-02-02 | 2001-09-11 | Signature Bioscience, Inc. | Method for detecting and classifying nucleic acid hybridization |
JP2002212537A (ja) * | 2001-01-24 | 2002-07-31 | Sony Chem Corp | 接着剤及び電気装置 |
KR100437474B1 (ko) * | 2001-04-04 | 2004-06-23 | 삼성에스디아이 주식회사 | 듀얼채널층을 갖는 박막 트랜지스터 및 그의 제조방법 |
DE10158149A1 (de) * | 2001-11-28 | 2003-06-18 | Bayer Ag | Silangruppen enthaltende Polymere |
WO2003052097A1 (en) * | 2001-12-19 | 2003-06-26 | Hitachi High-Technologies Corporation | Potentiometric dna microarray, process for producing the same and method of analyzing nucleic acid |
JP3952193B2 (ja) * | 2002-03-29 | 2007-08-01 | 学校法人早稲田大学 | 半導体センシングデバイス |
CN100392097C (zh) * | 2002-08-12 | 2008-06-04 | 株式会社日立高新技术 | 使用dna微阵列的核酸检测方法以及核酸检测装置 |
JP4092990B2 (ja) * | 2002-09-06 | 2008-05-28 | 株式会社日立製作所 | 生体および化学試料検査装置 |
JP2004184255A (ja) * | 2002-12-04 | 2004-07-02 | Arkray Inc | 分析装置 |
JP3903183B2 (ja) * | 2004-02-03 | 2007-04-11 | 独立行政法人物質・材料研究機構 | 遺伝子検出電界効果デバイスおよびこれを用いた遺伝子多型解析方法 |
WO2005090961A1 (ja) * | 2004-03-24 | 2005-09-29 | Japan Science And Technology Agency | 生体分子に関する形態及び情報をis−fetを利用して検出する測定法およびシステム |
US7888013B2 (en) * | 2004-08-27 | 2011-02-15 | National Institute For Materials Science | Method of analyzing DNA sequence using field-effect device, and base sequence analyzer |
US7838912B2 (en) * | 2004-09-30 | 2010-11-23 | Waseda University | Semiconductor sensing field effect transistor, semiconductor sensing device, semiconductor sensor chip and semiconductor sensing device |
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