JP4499406B2 - Intestinal function improving agent - Google Patents

Description

  The present invention relates to an intestinal function improving agent containing as an active ingredient a polysaccharide obtained through tea water or a hot water extraction step, and an oral application target comprising an effective amount of this intestinal function improving agent.

Indigestible carbohydrates, such as oligosaccharides and dietary fiber, are not degraded by human digestive enzymes, and thus escape digestive absorption by organs up to the small intestine and reach the large intestine. Some of the indigestible carbohydrates that reach the large intestine are assimilated by intestinal bacteria to produce short chain fatty acids such as acetic acid, propionic acid, and butyric acid, intestinal gas, and vitamins. When short-chain fatty acids increase in the intestine, the pH in the intestine decreases, and this action suppresses the growth of bad bacteria such as Clostridium perfringens and suppresses the production of spoilage products and bacterial toxins produced by the bad bacteria. It has been reported to produce an effect. This short-chain fatty acid is also known to stimulate the intestine, activate peristaltic movement, and promote defecation. Therefore, short-chain fatty acids improve the intestinal function by improving the intestinal environment and improving intestinal metabolism, so increasing the short-chain fatty acids in the intestine brings about an intestinal function improving action. Indigestible carbohydrates have been attracting attention for a long time (see Non-Patent Documents 1 and 2 below).
Indigestible carbohydrates having such characteristics have already been used in various foods and beverages and pharmaceuticals. For example, oligosaccharides have excellent physiological functions such as bifidobacteria growth promoting action in the intestine, intestinal regulating action, hard caries resistance, and mineral absorption promoting effect, and thus are attracting attention as one of functional sweeteners in the food field. Has been. In addition, for example, the combination of soluble dietary fiber and galactooligosaccharide improves the intestinal environment, lowers and reduces spoilage products, and is considered to be used as a food and medicine for the treatment and prevention of hepatic encephalopathy and colorectal cancer. A composition for improving the intestinal environment is known (see Patent Document 1 below). In addition, it is known that fructooligosaccharides have an action of proliferating intestinal bifidobacteria (see Patent Document 2 below). For example, constipation improvement consisting of cellulose and fructooligosaccharides, promotion of bowel movements, feasibility The composition for improvement (see Patent Document 3 below) aims to improve the bowel movement of dialysis patients due to renal failure. Inulin with fructose molecules linearly linked by β-2,1 bonds obtained from the roots and rhizomes of plants such as Jerusalem artichoke, chicory, burdock, and dahlia Is known to have an action to enhance the preparation and activity of intestinal bacteria and enhance the intestinal regulating action and intestinal cleansing (see Patent Document 4 below).
On the other hand, tea has been attracting attention because it has various excellent pharmacological actions. For example, tea catechin, which is a representative of its components, has been intestinalized by lowering the pH in the intestine, that is, the pH of feces. It is known that there is an effect of improving the environment (see Patent Document 5 below). Moreover, as an active ingredient other than catechin contained in tea, a polysaccharide derived from tea having a molecular weight of about 40,000 and L-arabinose, D-ribose, D-glucose as main constituent sugars has a hypoglycemic action. A hypoglycemic agent, an antidiabetic agent, and a health food that use this polysaccharide as an active ingredient have been proposed (see Patent Document 6 below). Furthermore, an antihyperlipidemic agent comprising this polysaccharide as an active ingredient has been proposed (see Patent Document 7 below). In addition, a plasma cholesterol-lowering agent has been proposed comprising a polysaccharide obtained by extracting tea leaves with water or warm water, in particular, a polysaccharide having a molecular weight of about 20,000 to about 200,000 as an active ingredient (Patent Document 8 below). See).
However, the elucidation of the action of tea components has not been sufficiently performed, and there are many unknown parts. Therefore, the search for a tea component having an intestinal function improving action is very significant.
JP-A-3-151854 JP 58-201980 A JP-A-1-319421 JP 2003-61592 A JP-A-6-166618 JP-A 63-308001 JP-A-2-286620 JP-A-6-80580 Hara Kenji, Biochemistry and Application of Bioactive Lipids-Short Chain Fatty Acids, Koshobo, December 2000 Satoshi Inami, 16 outside, revised edition, dietary fiber, Daiichi Publishing Co., Ltd., May 1995

  Therefore, an object of the present invention is to provide intestinal function improvement using a tea ingredient as an active ingredient.

  As a result of various studies in view of the above points, the present inventors have found that the polysaccharide obtained through the tea water or hot water extraction step has an excellent intestinal function improving action.

The present invention has been made on the basis of the above findings, and the intestinal function improving agent of the present invention, as described in claim 1, has a molecular weight of 400,000 or more obtained through a tea water or hot water extraction step. It is characterized by using a polysaccharide as an active ingredient .
Also, intestinal function-improving medicament of the present invention, as claimed in claim 2, characterized in that by blending the intestinal function improving agent an effective amount of claim 1, wherein.
The quasi drug for improving intestinal function according to the present invention is characterized in that, as described in claim 3 , an effective amount of the intestinal function improving agent described in claim 1 is blended.

  The intestinal function-improving agent in the present invention is a new intestinal function-improving agent comprising a polysaccharide obtained through tea water or hot water extraction process as an active ingredient, and is widely used for oral application objects such as foods and drinks and pharmaceuticals. It is highly versatile. The intestinal function-improving agent in the present invention improves intestinal function by increasing short-chain fatty acids in the intestine when ingested, and can exert constipation improving action by promoting bowel regulation or defecation. In addition, since the intestinal function improving agent of the present invention is a naturally-derived material that has been conventionally used for food, it is highly safe to the human body and continuously for a long time without having to worry about side effects. Can be taken.

  The polysaccharide (tea polysaccharide) obtained through the tea water or hot water extraction step used as an active ingredient of the intestinal function improving agent in the present invention is obtained by extracting tea with water or hot water. As long as it is a water-soluble polysaccharide or an insoluble polysaccharide, it is not particularly limited. In general, a polysaccharide is a saccharide other than a monosaccharide or oligosaccharide, and refers to a polymer having a polymerization degree of 10 or more. It should be noted that insoluble dietary fibers such as cellulose that are not obtained through the tea water or hot water extraction step are not included in the active ingredients of the present invention.

  The molecular weight of the tea polysaccharide is not particularly limited, and can exert an intestinal function improving effect at any molecular weight. Particularly, those having a molecular weight of 400,000 or more have excellent intestinal properties. This is preferable because it exhibits an internal function improving action. Tea polysaccharides having a desired molecular weight can be easily obtained by appropriately selecting a filtration membrane. Since the upper limit of the molecular weight is not particularly limited, any size polysaccharide may be used, but it is preferably one that can pass through a microfiltration membrane having a membrane pore size of 1.0 μm. When a component that cannot pass through a microfiltration membrane having a membrane pore diameter of 1.0 μm is included, it is not preferable because a certain effect or more cannot be obtained.

  In the present invention, the constituent sugars of the tea polysaccharide used as an active ingredient of the intestinal function improving agent are not particularly limited, but preferably contain at least galacturonic acid, galactose and arabinose as constituent sugars. . Further, the ratio of the constituent sugar contained in the tea polysaccharide is not particularly limited, but preferably the total constituent ratio of galacturonic acid, galactose and arabinose is 15% by weight or more of the total constituent sugar in the tea polysaccharide, More preferably, it is 30% by weight or more. In addition, the component ratio of each component of galacturonic acid, galactose, and arabinose is not particularly limited.

  Below, the manufacturing method of the intestinal function improving agent of this invention is demonstrated in detail.

  The tea in the present invention means any of leaves, stems, xylem, roots and fruits obtained from a tea tree (Camellia sinensis) belonging to the camellia family, or a mixture of two or more of these, raw or dried products. It does n’t matter. Moreover, it may be any of ready-made black tea, fermented tea such as puer tea, semi-fermented tea such as oolong tea and baked tea, non-fermented tea such as green tea, kettle roasted green tea, and roasted tea. It may be a mixture of more than one type.

  The tea polysaccharide used as an active ingredient of the intestinal function improving agent of the present invention can be obtained from tea water or hot water extract, for example. This tea extract can be prepared by subjecting the tea as it is or after pulverization to an extraction operation, or after drying and pulverizing if necessary, followed by an extraction operation. The dried pulverized product can be prepared by drying the tea leaves, stems, xylem, roots and fruits as they are and then pulverizing them, or cutting them raw and then drying them.

  As an extraction method, a general method using water or hot water can be employed. For example, tea (as it is or coarsely divided or shredded product) in water, hot water or hot water, or a dry pulverized product thereof. Examples include a method of immersing (such as powder) and a method of performing extraction while heating and stirring. The obtained extract may be used as it is after removing solids by filtration or centrifugation as necessary, and may be used as it is, or it may be further concentrated, or it may be freeze-dried or spray-dried. May be used after being powdered. Furthermore, you may remove and use components other than tea polysaccharides, such as catechins. In general, commercially available tea extracts may be used. Examples of such commercially available products include Tokyo Food Techno Co., Ltd. “Polyphenone”, Taiyo Kagaku Co., Ltd. “Sunphenon”, and ITO EN Co., Ltd. And the like.

  To prepare a tea polysaccharide from a tea extract, for example, a water-containing organic solvent such as 50-90% ethanol aqueous solution or an organic solvent such as ethanol is added to the tea extract and stirred well. Collect by centrifugation. Then, a water-containing organic solvent such as the above ethanol aqueous solution or an organic solvent such as ethanol is added to the insoluble matter again, and after sufficient stirring, the insoluble matter is collected using centrifugation or the like. After repeating this operation several times, if the collected insoluble matter is dried by a method such as vacuum drying, freeze drying, spray drying or the like, crude tea polysaccharides can be obtained.

  In order to purify the tea polysaccharide from the crude tea polysaccharide, first, water or the like is added to the obtained crude tea polysaccharide and stirred well to obtain a suspension. A method of separating the suspension according to the molecular weight, such as microfiltration, ultrafiltration or gel filtration, is adopted, and a filtration membrane or gel filtration material is appropriately selected according to the desired molecular weight. This can be done by fractionating. For example, in order to purify a tea polysaccharide having a molecular weight of 400,000 or more, the suspension may be permeated through an ultrafiltration membrane having a fractional molecular weight of 400,000. For the desired molecular weight tea polysaccharide, first, a desired molecular weight fraction is obtained from the tea extract, and a water-containing organic solvent such as 50-90% ethanol aqueous solution or an organic solvent such as ethanol is added to this fraction. You may make it obtain.

  The components once fractionated can be further finely separated and purified by changing the size of the filtration membrane or gel filtration material. For example, it is possible to fractionate more than 2 million components from components having a molecular weight of 400,000 or more, or fractionate components that can pass through a microfiltration membrane having a membrane pore diameter of 1.0 μm and have a molecular weight of 400,000 or more. Is possible.

  As described above, the tea polysaccharide purified from the crude tea polysaccharide can be obtained by appropriately selecting a filtration membrane or gel filtration material in accordance with the size of the tea polysaccharide to be obtained.

  Furthermore, after suspending the crude tea polysaccharide in water, warm water or hot water, the precipitate is collected by centrifugation, filtration, etc., and the collected precipitate is washed by repeating the above operation, Insoluble tea polysaccharides can be purified from the crude tea polysaccharides. Water-soluble tea polysaccharides can be recovered from the water-soluble fraction obtained by the above operation. In addition, for example, the crude tea polysaccharide can be fractionated in advance into a tea polysaccharide fraction having a molecular weight of 400,000 or more, and then the insoluble polysaccharide can be collected by centrifugation or filtration. The insoluble tea polysaccharide and water-soluble tea polysaccharide obtained in this way may be used alone or in a mixture at an appropriate ratio.

  The tea polysaccharide obtained as described above can be dried and powdered by a method such as freeze drying or spray drying, if necessary. Moreover, as another method, ethanol can be added to these so that it may become 50-90%, a tea polysaccharide can be precipitated, and it can also be made into powder by drying after collect | recovering deposits.

  Since the intestinal function improving agent prepared by the method as described above is a component obtained from tea that is drunk daily in large quantities, it is highly safe and can be mass-produced. Further, the production can be performed relatively easily.

  As the intestinal function improving agent of the present invention, the mixture of tea polysaccharides obtained by the method as described above may be used as it is, or the tea polysaccharide is used as an ion exchange resin, an adsorption resin, a gel filtration material or the like. Individual tea polysaccharides obtained by further fine purification may be used. Further, two or more kinds of tea polysaccharides obtained by purification may be used in combination at a desired mixing ratio. Regardless of how it is used, the intestinal function improvement of the present invention reduces the intestinal pH by improving the intestinal environment and intestinal metabolism based on the increase action of short-chain fatty acids in the intestine, By suppressing the growth of bad bacteria such as Clostridium perfringens and suppressing the production of spoilage products and bacterial toxins produced by the bad bacteria, it is possible to exert an effect of improving constipation by promoting bowel regulation or defecation. Here, the short-chain fatty acid may be anything that is generally interpreted, and is selected from acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, lactic acid, malic acid, succinic acid, and the like. It may be interpreted as meaning at least one, or may be interpreted as meaning a total short-chain fatty acid summed up.

  The intestinal function improving agent of the present invention exhibits an excellent intestinal function improving effect by blending an effective amount in an oral application target. In the present invention, the oral application target may be anything as long as it can be contained in the mouth, but is preferably a food / drink, a pharmaceutical, a quasi-drug, or the like that allows the tea polysaccharide to reach the intestine. Can be exemplified.

  The oral application target of the present invention may be in any form as long as it can be blended with a tea polysaccharide that is an active ingredient of an intestinal function improving agent, for example, an aqueous solution, a turbid solution, or an emulsification. It may be in a liquid form such as a product, in a semi-solid form such as a gel or paste, or in a solid form such as powder, granule, capsule or tablet.

  Among the oral application objects in the present invention, as foods and drinks, for example, instant foods (immediate noodles, cup noodles, retort / cooked food, cooking canned food, microwave food, instant miso soup / soup, canned soup, freeze-dried food, etc. ), Carbonated drinks, citrus fruit juices (grapefruit, orange, lemon, etc.), juice drinks, soft drinks with juice, citrus fruit drinks and fruit drinks, tomatoes, peppers, celery, cucumbers, carrots, potatoes, asparagus Vegetable beverages including vegetables such as soy milk and soy milk beverages, coffee beverages, tea beverages, powdered beverages, concentrated beverages, sports beverages, nutritional beverages, alcoholic beverages, tobacco beverages and other favorite beverages and luxury products, bread, macaroni and spaghetti, Noodles, cake mix, deep-fried flour, bread crumbs, gyoza peel and other flour products, caramel・ Candies, chewing gum, chocolate, cookies / biscuits, cakes / pies, snacks / crackers, Japanese confectionery / rice confectionery / bean confectionery, dessert confectionery, soy sauce, miso, sauces, tomato processed seasonings, mirin, vinegar , Basic seasonings such as sweeteners, flavor seasonings, cooking mixes, curry ingredients, sauces, dressings, noodle soups, spices and other complex seasonings and foods, butter, margarines, mayonnaise, Fats and oils such as vegetable oil, milk / processed milk, milk drinks, yogurts, lactic acid bacteria drinks, cheese, ice creams, prepared powdered milk, milk and other dairy products such as cream, frozen material, semi-cooked frozen food, cooked frozen Frozen foods such as food, canned fish, canned fruits and pastes, fish ham and sausage, marine products, marine delicacy Processed fishery products such as dried fishery products, boiled fish, canned livestock cans / pastes, canned meat, canned fruits, jams / marmalades, pickles / boiled beans, dried agricultural products, processed cereals (cereal processed products) , Baby food, commercial foods such as sprinkles and ochazukenori.

  In addition, among the oral applied objects of the present invention, the pharmaceutical is not particularly limited as long as it can be contained in the mouth with the pharmaceuticals contained in the Japanese Pharmacopoeia. , Liquids, extracts, elixirs, capsules, granules, pills, powders, spirits, tablets, syrups, dip / decoction, troches, fragrances, limonases and the like. The quasi-drug is not particularly limited as long as it is a quasi-drug specified by the Minister of Health, Labor and Welfare and can be included in the mouth. For example, oral liquids, health drinks, disinfectants, antiseptics, vitamin-containing health care Agents and the like.

  The method of blending the intestinal function improving agent of the present invention into the oral application target is not particularly limited. For example, it may be performed by adding the tea polysaccharide of the present invention in a powder form or a liquid form in the preparation stage of the oral application target and homogenizing it. In addition, when preparing the oral application target, a coloring agent, an antioxidant, a fragrance, a corrigent, a surfactant, a solubilizing agent, a preservative, a sweetener, and the like may be used in combination as appropriate. Good.

  The blending amount of the intestinal function improving agent of the present invention with respect to the oral application target is not particularly limited, and it is preferable to appropriately set the blending amount according to the target oral application target. Generally, it is preferably 0.1% by weight or more in the final product, more preferably 1% by weight or more, and most preferably 5% by weight or more. The upper limit of the amount is preferably 50% by weight in the final product. When the blending amount of the intestinal function improving agent is less than 0.1% by weight, the target intestinal function improving effect may not be expected. On the other hand, when an excessive amount is blended, when the oral application target is a food or drink, the original taste of the food or drink is impaired, or a certain effect or more cannot be obtained, which is economically disadvantageous. There is.

  The intestinal function-improving agent of the present invention may be ingested by being mixed with the oral application target as described above, but can also be ingested as it is. The intake amount varies depending on the intake form, age, weight, etc., but is not particularly limited.

  The intestinal function improving agent of the present invention does not cause any problems even when used in combination with conventionally known substances having an intestinal function improving action, such as oligosaccharides and tea catechins. When used in combination with these substances, a better intestinal function improving effect can be expected.

  Hereinafter, the present invention will be described in more detail with reference to production examples, test examples and formulation examples. However, the present invention is not limited to this.

Production Example 1: Preparation of Crude Tea Polysaccharide Obtained through Hot Water Extraction Step of Green Tea 5 kg of green tea was added to 50 L of hot water at 90 ° C. and extracted for 30 minutes with occasional stirring. The obtained extract was filtered, concentrated under reduced pressure, and then dried by spray drying to obtain about 1.5 kg of a powdery green tea extract.
2 L of 70% ethanol was added to 1.5 kg of the green tea extract obtained in this manner and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 200 g of a crude tea polysaccharide as an insoluble material.

Production Example 2: Preparation of purified tea polysaccharide obtained through a hot water extraction step of green tea (Part 1)
2 L of 70% ethanol was added to 1.5 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 350 g of a crude tea polysaccharide as an insoluble material.
5 L of water was added to 350 g of this crude tea polysaccharide and stirred well to obtain a suspension. This suspension was subjected to ultrafiltration membrane (polysulfone membrane PS / 400K, manufactured by Spectrum) having a molecular weight cut off of 400,000. Thus, it was fractionated into a component having a molecular weight of 400,000 or more and a component having a molecular weight of less than 400,000. Each component obtained was concentrated and freeze-dried to obtain 222 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 102 g of a tea polysaccharide fraction having a molecular weight of less than 400,000. The ratio of the constituent sugar contained in the tea polysaccharide having a molecular weight of 400,000 or more was such that the total constituent ratio of galacturonic acid, galactose and arabinose was about 30% by weight of the whole constituent sugar in the tea polysaccharide.

Production Example 3: Preparation of purified tea polysaccharide obtained through a hot water extraction step of green tea (part 2)
10 L of 70% ethanol was added to 2 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. The insoluble matter was collected by centrifugation, 4 L of 70% ethanol was added to the insoluble matter, and after sufficient stirring, the insoluble matter was collected by centrifugation. This operation was further repeated twice to obtain about 256 g of crude tea polysaccharide as an insoluble material.
10 g of water is added to 256 g of this crude tea polysaccharide and stirred well to obtain a suspension, and the molecular weight is less than 400,000 using an ultrafiltration membrane having a fractional molecular weight of 400,000 (polysulfone membrane PS / 400K, manufactured by Spectrum). These fractions were removed and fractions having a molecular weight of 400,000 or more were concentrated and collected.
Next, a precipitate was collected by centrifuging the obtained fraction having a molecular weight of 400,000 or more. The operation of washing the precipitate with 2 L of water was repeated twice and then freeze-dried to obtain about 118 g of insoluble tea polysaccharide having a molecular weight of 400,000 or more.
On the other hand, the water-soluble fraction obtained upon centrifugation was freeze-dried to obtain about 56 g of water-soluble tea polysaccharide having a molecular weight of 400,000 or more.
Further, the component having a molecular weight of less than 400,000 obtained by ultrafiltration was concentrated and freeze-dried to obtain about 50 g of tea polysaccharide having a molecular weight of less than 400,000.

Production Example 4: Preparation of purified tea polysaccharide obtained through a hot water extraction process of green tea (part 3)
2 L of 70% ethanol was added to 1.5 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 350 g of a crude tea polysaccharide as an insoluble material.
5 L of water was added to 350 g of this crude tea polysaccharide and stirred well to obtain a suspension. This suspension was used with a microfiltration membrane (polypropylene membrane TCP-1, manufactured by Advantech) having a membrane pore size of 1.0 μm. Components larger than 1.0 μm were removed. The obtained permeate was fractionated into a component having a molecular weight of 400,000 or more and a component having a molecular weight of less than 400,000 using an ultrafiltration membrane having a fractional molecular weight of 400,000 (polysulfone membrane PS / 400K, manufactured by Spectrum). Each component obtained was concentrated and freeze-dried to obtain 222 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 102 g of a tea polysaccharide fraction having a molecular weight of less than 400,000.

Production Example 5 Preparation of Purified Tea Polysaccharide Obtained through Hot Water Extraction Step of Oolong Tea 5 kg of Chinese Oolong tea was added to 50 L of hot water at 90 ° C. and extracted for 30 minutes with occasional stirring. The obtained extract was filtered, concentrated under reduced pressure, and then dried by spray drying to obtain about 1.5 kg of powdered oolong tea extract.
2 L of 70% ethanol was added to 1.5 kg of the oolong tea extract thus obtained and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 200 g of a crude tea polysaccharide as an insoluble material.
To 200 g of this crude tea polysaccharide, 4 L of water was added and stirred well to obtain a suspension. This suspension was subjected to ultrafiltration membrane (polysulfone membrane PS / 400K, And then concentrated and freeze-dried to obtain 87 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 90 g of a tea polysaccharide fraction having a molecular weight of less than 400,000.

Production Example 6: Preparation of purified tea polysaccharide obtained through hot water extraction step of black tea 5 kg of Indian black tea was added to 50 L of hot water at 90 ° C. and extracted for 30 minutes with occasional stirring. The obtained extract was filtered, concentrated under reduced pressure, and then dried by spray drying to obtain about 1.5 kg of a powdery black tea extract.
To 1.5 kg of the black tea extract thus obtained, 2 L of 70% ethanol was added and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 210 g of a crude tea polysaccharide as an insoluble material.
To 200 g of this crude tea polysaccharide, 4 L of water was added and stirred well to obtain a suspension. This suspension was subjected to ultrafiltration membrane (polysulfone membrane PS / 400K, And then concentrated and freeze-dried to obtain 80 g of a tea polysaccharide fraction having a molecular weight of 400,000 or more and 96 g of a tea polysaccharide fraction having a molecular weight of less than 400,000.

Production Example 7: Preparation of purified tea polysaccharide obtained through a hot water extraction step of green tea (Part 4)
To 1 kg of crushed green tea, 10 L of 70% ethanol aqueous solution was added and stirred at room temperature for 12 hours. Insoluble components were collected by filtration, and the same amount of 70% aqueous ethanol solution was added again and stirred for 8 hours. To the obtained insoluble component, 5 L of 70% ethanol aqueous solution was added and stirred for 4 hours to wash the insoluble component. This operation was repeated once more to obtain 500 g of ethanol-treated green tea leaves.
500 g of ethanol-treated green tea leaves were added to 5 L of hot water at 90 ° C., and extracted for 30 minutes with occasional stirring. The resulting extract 3L was treated with an ultrafiltration membrane having a fractional molecular weight of 400,000 or more (polysulfone membrane PS / 400K, manufactured by Spectrum), and 40 g of a water-soluble tea polysaccharide having a molecular weight of 400,000 or more and a molecular weight. 50 g of tea polysaccharide less than 400,000 was obtained.

Production Example 8: Preparation of purified tea polysaccharide obtained through the hot water extraction step of green tea (part 5)
2 L of 70% ethanol was added to 1.5 kg of green tea hot water extract (Polyphenon G, manufactured by Tokyo Food Techno Co., Ltd.) and stirred well. Insoluble matters were collected by centrifugation, 2 L of 70% ethanol was added to the insoluble matters, and after sufficient stirring, the insoluble matters were collected by centrifugation. This operation was further repeated twice to obtain about 300 g of a crude tea polysaccharide as an insoluble material.
5 L of water is added to 300 g of this crude tea polysaccharide and stirred well to form a suspension. This suspension is subjected to a molecular weight of 200 using an ultrafiltration membrane (cellulose ester membrane, manufactured by Spectrum) having a molecular weight cut off of 2 million. It was fractionated into more than 10,000 components and less than 2 million components. Each component obtained was concentrated and freeze-dried to obtain 70 g of a tea polysaccharide fraction having a molecular weight of 2 million or more and 171 g of a tea polysaccharide fraction having a molecular weight of less than 2 million.

Test Example 1: Defecation promoting action test by tea polysaccharide obtained through hot water extraction process of green tea (Part 1)
The fecal excretion promoting effect of the crude tea polysaccharide described in Production Example 1 was examined according to the following test method.
(Test method)
Four-week-old male SD rats were preliminarily raised on a solid diet for 3 days, then divided into groups so that the average body weight was equal, and each group was fed a diet having the composition shown in Table 1 for 10 days. The crude tea polysaccharide described in Production Example 1 was added to the test group, and sucrose was added to the control group instead of the crude tea polysaccharide. During this time, food and water were freely consumed. For each group, the feces during the 10-day breeding period of all the individuals were collected, the total fecal weight (dry weight) was measured, and this was divided by the number of individuals to calculate the fecal weight per 10 days of one individual. The fecal weight per day was calculated by dividing the fecal weight by the number of days. The calculation results are shown in Table 2. The breeding room temperature was 23 ° C. with a light period of 8:00 to 20:00 and a dark period of 20:00 to 8:00.

(Test results)
As is clear from Table 2, the fecal weight significantly increased in the test group ingested with the crude tea polysaccharide as compared with the control group. From this, the ingestion of the crude tea polysaccharide significantly promoted defecation and exhibited an excellent intestinal function improving effect.

Test Example 2: Defecation promoting action test by tea polysaccharide obtained through hot water extraction process of green tea (part 2)
The fecal excretion promoting effect of the tea polysaccharide having a molecular weight of 400,000 or more and the tea polysaccharide having a molecular weight of less than 400,000 described in Production Example 2 was examined according to the following test method.
(Test method)
Four-week-old male SD rats were preliminarily raised on a solid diet for 3 days, and then grouped so that the average body weight was equal, and each group was fed a diet having the composition shown in Table 3 for 10 days. Test group 1 contains tea polysaccharides with a molecular weight of less than 400,000 as described in Production Example 2, test group 2 with tea polysaccharides with a molecular weight of 400,000 or more as described in Production Example 2, and control group with tea Sucrose was added instead of sugars. During this time, food and water were freely consumed. For each group, the feces during the 10-day breeding period of all the individuals were collected, the total fecal weight (dry weight) was measured, and this was divided by the number of individuals to calculate the fecal weight per 10 days of one individual. The fecal weight per day was calculated by dividing the fecal weight by the number of days. Table 4 shows the calculation results. The breeding room temperature was 23 ° C. with a light period of 8:00 to 20:00 and a dark period of 20:00 to 8:00.

(Test results)
As is clear from Table 4, both the test group 1 ingested tea polysaccharides with a molecular weight of less than 400,000 and the test group 2 ingested tea polysaccharides with a molecular weight of 400,000 or more compared to the control group. Although it increased significantly, it increased especially especially in the test group 2 which ingested the tea polysaccharide with a molecular weight of 400,000 or more. From this, it can be seen that defecation is sufficiently promoted regardless of the molecular weight of the tea polysaccharide, and in particular, ingestion of tea polysaccharide having a molecular weight of 400,000 or more exhibits an excellent defecation promoting effect. It was.
In addition to green tea-derived tea polysaccharides, the feces weight was measured using oolong tea-derived or black tea-derived tea polysaccharides. In the test group ingested with tea polysaccharides, the fecal weight was significantly higher than the control group. Increased. From this, it was confirmed that the tea polysaccharide used in the present invention has an excellent intestinal function improving action such as promoting defecation without being limited to the type of tea.

Test Example 3: Test for promoting excretion of short-chain fatty acids in feces by tea polysaccharide obtained through a hot water extraction process of green tea Tea polysaccharide having a molecular weight of 400,000 or more and tea poly having a molecular weight of less than 400,000 described in Production Example 2 The effect of promoting the excretion of short-chain fatty acids in feces by saccharides was examined according to the following test method.
(Test method)
Four-week-old male SD rats were bred in the same manner as in Test Example 2. After the breeding, feces were collected, and the concentration of each short chain fatty acid in the feces was analyzed with a gas chromatograph (Agilent 6890 Series GC System; 6890A). GC analysis conditions are shown in Table 5. Table 6 shows the concentrations of acetic acid, propionic acid, butyric acid and total short chain fatty acids for the analysis results of short chain fatty acids in feces. The total short-chain fatty acid referred to here is a total of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid and valeric acid, and valeric acid was excluded from the test results because the measured amount was very small. The breeding room temperature was 23 ° C. with a light period of 8:00 to 20:00 and a dark period of 20:00 to 8:00.

(Test results)
As is clear from Table 6, both test group 1 ingested tea polysaccharides with a molecular weight of less than 400,000 and test group 2 ingested tea polysaccharides with a molecular weight of 400,000 or more were acetic acid and propionic acid in feces. In addition, all values of butyric acid and total short-chain fatty acids increased compared to the control group, and in particular, significant differences were observed in acetic acid, propionic acid and total short-chain fatty acids. Thus, regardless of the molecular weight of the tea polysaccharide, the concentration of short-chain fatty acids in the feces was increased, and an excellent intestinal function improving effect was exhibited. Increasing short chain fatty acids in the intestine shortens the digestion transit time of the intestinal contents (Oufir, LE, Flourie, B., Des Varannes, SB, Barry, JL, Cloarec, D., Bornet, F., Galmiche, JP, Gut, vol.38, p.870, 1996), it has been reported that shortening the digestion transit time leads to an increase in the amount of defecation (Burkitt, D., Walker, ARP, Painter, NS, J. Am. Med. Assoc., Vol. 229, p. 1069, 1974). As is clear from this, the tea polysaccharide used in the present invention has the effect of remarkably promoting defecation by producing a short chain fatty acid partly assimilated by intestinal bacteria. It was found that it can be expected as a constipation improving agent.

  Below, the formulation example which mix | blended the intestinal function improving agent in this invention with the oral-applicable object is shown. The food / beverage products containing the intestinal function improving agent of the present invention can be ingested deliciously without impairing the original taste / fragrance of the food / beverage products.

Formulation Example 1: Green tea beverage containing an intestinal function improving agent 900 mL of water was heated to 60 ° C., and 30 g of green tea (gold) was added thereto and extracted for 6 minutes. After removing the tea husk with a 30-mesh strainer and cooling to 30 ° C. or lower, it is clarified by filter paper filtration (industrial filter paper No. 26: ADVANTEC, collected particle size = 3 μm), and 760 mL of extract solution Got. This green tea extract is diluted with ion-exchanged water to a drinking concentration (catechin concentration 60 mg%), L-ascorbic acid is added to 0.03% by weight, and then pH 6.1 to 6. It adjusted to the range of 3 and was set as the preparation liquid. To this blended solution, 5% by weight of the green tea-derived tea polysaccharide obtained in Production Example 1 was added and stirred well. These were hot-pack filled under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 10 minutes (F ₀ = 10 or higher) by retort sterilization. After cooling these to room temperature, a green tea beverage containing an intestinal function improving agent was obtained.

Formulation Example 2: Oolong tea beverage containing an intestinal function improving agent 900 mL of water was heated to 80 ° C., and 30 g of Oolong tea (Oolong G) was added thereto and extracted for 6 minutes. The tea shell was removed, cooled and clarified in the same manner as in Formulation Example 1 to obtain 810 mL of oolong tea extract. This oolong tea extract is diluted with ion-exchanged water so as to have a drinking concentration (catechin concentration of 50 mg%), L-ascorbic acid is added to 0.03% by weight, and then pH 6.1-6. It adjusted to the range of 3 and was set as the preparation liquid. To this blended solution, 5% by weight of the tea polysaccharide derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 was added and stirred well. These were hot-pack filled under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 10 minutes (F ₀ = 10 or higher) by retort sterilization. After these were cooled to room temperature, Oolong tea drink containing an intestinal function improving agent was obtained.

Formulation Example 3: Black Tea Beverage Containing Intestinal Function Improvement Agent Prepared in the same manner as Formulation Example 2 except that Oolong Tea (Oolong G) in Formulation Example 2 was changed to black tea (Nuwaya Lemon Tea) to improve intestinal function A tea beverage containing the agent was obtained.

Formulation Example 4: Barley tea beverage containing an intestinal function improving agent 800 mL of water was heated to 90 ° C., and 40 g of barley tea raw barley was added thereto and extracted for 30 minutes. After removing the tea husk with a 30 mesh strainer and cooling to 30 ° C. or lower, clarification was performed by filter paper filtration (industrial filter paper No. 2: manufactured by ADVANTEC) to obtain 720 mL of an extract. This barley tea extract is diluted with ion-exchanged water so as to have a drinking concentration (Brix 0.4 °), L-ascorbic acid is added to 0.03% by weight, and the pH is 6.0 with sodium bicarbonate. To prepare a preparation liquid. To this blended solution, 5% by weight of the tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 6 was added and stirred well. These were hot-packed under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 20 minutes by retort sterilization. After cooling them to room temperature, a barley tea beverage containing an intestinal function improving agent was obtained.

Formulation Example 5: Mixed tea beverage containing intestinal function improving agent 900 mL of water is heated to 90 ° C., and mixed tea (mixing ratio: 65% oolong tea, 20% black tea, 5% jasmine, 4% crust, hibiscus 4 % And banaba 2%) was added and extracted for 10 minutes. After removing the tea husk with a 30-mesh strainer and cooling to 30 ° C. or lower, clarification was performed by filter paper filtration (industrial filter paper No. 2: manufactured by ADVANTEC) to obtain 820 mL of an extract. This mixed tea extract is diluted with ion-exchanged water to a drinking concentration (Brix 0.2 °), L-ascorbic acid is added to 0.03% by weight, and then pH 6.0 with sodium bicarbonate is added. It adjusted to the range and it was set as the preparation liquid. To this blended liquid, 5% by weight of green tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 2 was added and stirred well. These were hot-packed under a temperature condition of 80 ° C. or higher, and then sterilized at 121 ° C. for 20 minutes by retort sterilization. After cooling these to room temperature, a mixed tea beverage containing an intestinal function improving agent was obtained.

Formulation Example 6: Orange 50% juice drink containing intestinal function improver 6 times concentrated orange juice 84g
Fructose glucose liquid sugar 9.7g
Citric acid 0.06g
L-ascorbic acid 0.022g
5 g tea polysaccharide obtained in Production Example 5
Fragrance appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL. This was filled in a container and sterilized by heating at 65 ° C. for 10 minutes to obtain an orange 50% fruit juice drink containing tea polysaccharides derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5.

Formulation Example 7: Orange 100% fruit juice drink containing intestinal function improving agent 1/6 concentrated orange fruit juice 168 g
10 g tea polysaccharide obtained in Production Example 6
Fragrance appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL. This was filled in a container and sterilized by heating at 65 ° C. for 10 minutes to obtain a 100% orange juice drink containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 8: Peach 50% fruit juice drink containing intestinal function improving agent 1/4 concentrated peach juice 131g
Fructose glucose liquid sugar 9.7g
Citric acid 0.06g
L-ascorbic acid 0.022g
5 g tea polysaccharide obtained in Production Example 2
Fragrance appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL. This was filled in a container and sterilized by heating at 65 ° C. for 10 minutes to obtain a peach 50% fruit juice drink containing tea polysaccharides derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2.

Formulation Example 9: Grapefruit juice containing an intestinal function improving agent Commercially available grapefruit juice (Sanquist grapefruit juice concentrated and reduced 100%: trade name of Sanquist) Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 of the present invention Grapefruit juice containing an intestinal function improving agent was prepared by adding 3% by weight of the derived tea polysaccharide.

Formulation Example 10: Grapefruit juice containing pulp containing an intestinal function improving agent A commercially available grapefruit juice containing pulp (Gokuri: trade name of Suntory Ltd.) derived from black tea having a molecular weight of 400,000 or more obtained in Production Example 6 of the present invention Grapefruit juice containing pulp containing 3% by weight of tea polysaccharide and containing an intestinal function improving agent was prepared.

Formulation Example 11: Vegetable juice containing an intestinal function improving agent Commercially available vegetable juice (Kagome vegetable juice: trade name of Kagome Co., Ltd.), obtained from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 of the present invention 5% by weight of saccharide was added to prepare a vegetable juice containing an intestinal function improving agent.

Formulation example 12: Coffee beverage containing an intestinal function improving agent Commercial coffee beverage (black bottle sugar-free: trade name of DyDo DRINCO) derived from oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 of the present invention 1% by weight of tea polysaccharide was added to prepare a coffee drink containing an intestinal function improving agent.

Formulation Example 13: Sports drink containing intestinal function improving agent Tea polysaccharide obtained in Production Example 6 20 g
Vitamin B ₁ hydrochloride 0.45mg
Vitamin B ₂ 0.2mg
Vitamin C 10mg
Niacin 0.8mg
Pantothenic acid Ca 0.22mg
Ammonium iron citrate 12.57mg
Citric acid 100mg
Fructose 2.5g
Ion-exchanged water was added to the components described above to make a total volume of 200 mL, and a sports drink containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6 was prepared.

Formulation Example 14: Cola granulated sugar containing an intestinal function improving agent 5 g
Cola base 0.3g
Citric acid 0.05g
5 g tea polysaccharide obtained in Production Example 8
Carbonated water was added to the components described above to make the total amount 100 mL, and a cola containing green tea-derived tea polysaccharides having a molecular weight of 2 million or more obtained in Production Example 8 was prepared.

Formulation Example 15: 1.7 g milk shake butter containing an intestinal function improving agent
Nonfat dry milk 5.5g
10 g tea polysaccharide obtained in Production Example 6
5g sugar
Sorbitol 4g
Emulsification stabilizer 0.7g
Ion exchange water was added to the components described above to make a total volume of 100 mL, and the mixture was dissolved by heating to 80 ° C. After homogenizing the milk fat, it was aged at 5 ° C. until the next day. After freezing this, it was rapidly cooled to −40 ° C. and mixed well to prepare a milk shake containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 16: Drink yogurt containing an intestinal function improver Brewed skim milk (fermented skim milk and ground) 40 g
13g sugar
Stabilizer 0.35g
Fragrance 0.05g
10 g tea polysaccharide obtained in Production Example 6
Ion exchange water was added to the components described above to make a total amount of 100 g, and mixed and dissolved. This was homogenized with a homogenizer to prepare a drink yogurt containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 17: Red wine containing an intestinal function improver A tea derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 of the present invention in a commercially available red wine (Delica Maison Red / Light Type: trade name of Suntory) A red wine containing 1% by weight of polysaccharide and containing an intestinal function improving agent was prepared.

Formulation Example 18: Beer containing an intestinal function improver A tea polysaccharide derived from Oolong tea having a molecular weight of 400,000 or more obtained in Production Example 5 of the present invention is added to a commercially available beer (Kirin Lager: trade name of Kirin Beer). A beer containing 1% by weight and containing an intestinal function improving agent was prepared.

Formulation Example 19: Soymilk drink containing intestinal function improving agent Soybean flour aqueous solution 500 g
90g sugar
5 times concentrated peach juice 20g
Citric acid 3g
Pectin 3g
Milk flavor 0.5g
1g peach flavor
10 g tea polysaccharide obtained in Production Example 6
The components described above were mixed, and ion-exchanged water was added to make a total volume of 1000 mL. The mixture was sterilized at 92 ° C. for 1 to 3 seconds, and then filled into a container at 87 ° C. to prepare a soy milk beverage containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6. .

Formulation Example 20: 30 g of soup corn starch containing an intestinal function improving agent
5 g tea polysaccharide obtained in Production Example 2
5g sugar
5g salt
Non-fat dry milk 30g
1g meat extract powder
4g of HAP powder
5g sodium glutamate
0.2g white pepper
Celery powder 0.01g
Onion extract powder 5g
Margarine 5g
5g dried carrot
0.3g dried parsley
The ingredients described above are powder-mixed in an instant soup formulation, 200 mL of water is added to 21.5 g of this mixture, heated for 3 to 5 minutes with good mixing, and derived from green tea with a molecular weight of 400,000 or more obtained in Production Example 2. A powder potage soup containing tea polysaccharides was prepared.

Formulation Example 21: Beef stew beef 110 g containing an intestinal function improving agent
Potato (6 slices) 60g
Carrot (6 slices) 50g
215g domiglas sauce
5 g tea polysaccharide obtained in Production Example 5
The above-mentioned materials were added to No. 4 can and mixed well. This was sterilized by retort at 115 ° C. for 90 minutes to prepare a beef stew containing tea polysaccharides derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5.

Formulation Example 22: Retort curry containing intestinal function improving agent
0.5g salt
5.2g butter
8.4g potato
Onion 12.4g
Carrot 2.5g
3.6g flour
Curry powder 0.5g
Spice 0.5g
5 g tea polysaccharide obtained in Production Example 5
Rose meat and vegetables were fried separately in a frying pan and placed in a pan. The retort curry containing tea polysaccharides derived from Oolong tea with a molecular weight of 400,000 or more obtained in Production Example 5 was prepared by frying the powder to make brown roux and simmering the whole in a pan.

Formulation Example 23: Non-oil dressing containing intestinal function improving agent Vinegar 30.8 g
4g sugar
Salt 0.5g
8.4g thin soy sauce
Dashi soup 26.3g
20g powdered candy
10 g tea polysaccharide obtained in Production Example 6
After mixing the liquid raw material, the powder raw material was dissolved to prepare a non-oil dressing containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6.

Formulation Example 24: Noodle soup containing an intestinal function improving agent (double concentration type)
70mL soy sauce
20g sugar
Mirin 5mL
Boiled bonito broth 9g
Nucleic acid seasoning 3g
2g of salt
5 g tea polysaccharide obtained in Production Example 8
Caramel appropriate amount The above-described components were dissolved in ion-exchanged water to make a total amount of 1000 mL, and a double-concentrated noodle soup containing tea polysaccharides derived from green tea having a molecular weight of 2 million or more obtained in Production Example 8 was prepared.

Formulation Example 25: Yakiniku sauce containing intestinal function improving agent Soy sauce 900 mL
500g miso
350g sugar
Onion paste 100g
200g apple paste
60g sesame oil
250g garlic paste
20g white sesame paste
50 g of tea polysaccharide obtained in Production Example 2
The ingredients described above were dissolved in ion-exchanged water to make a total amount of 2000 mL, and a yakiniku sauce containing green tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

Formulation Example 26: Mayonnaise containing intestinal function improver Egg yolk One egg Vinegar 1 teaspoon Salt Suitable amount Salad oil 120mL
Appropriate amount of sugar 5 g of tea polysaccharide obtained in Production Example 3
The ingredients described above were mixed while being slowly added according to a conventional method to prepare mayonnaise containing insoluble tea polysaccharide derived from green tea obtained in Production Example 3.

Formulation Example 27: Hard yogurt containing intestinal function improving agent Nonfat milk 90.9 g
5g sugar
5 g tea polysaccharide obtained in Production Example 3
Stevioside 0.05g
Fragrance Small amount Curing agent Small amount After adding a curing agent to skim milk, 3% starter was inoculated and refrigerated when the acidity reached 0.7%. Other raw materials were stirred and mixed, and refrigerated again to prepare hard yogurt containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 28: 7.5 g of ice cream butter containing an intestinal function improving agent
Nonfat dry milk 5g
10g sugar
5 g tea polysaccharide obtained in Production Example 3
Emulsification stabilizer 0.7g
Flour candy (DE 8) 7g
Ion exchange water was added to the components described above to make the total amount 100 g and mixed well. This was heated to 70 ° C., stirred with a homomixer, and then homogenized with a homogenizer. The ice cream containing the insoluble tea polysaccharide derived from the green tea obtained in Production Example 3 was prepared by aging in a refrigerator for 1 day and then rapidly cooling to −40 ° C. after freezing.

Formulation Example 29: Coffee whitener (powder type) containing an intestinal function improving agent
Soybean hardened oil 50g
25g Minamata
Emulsifier 0.84g
Cream flavor 0.16g
20 g of tea polysaccharide obtained in Production Example 2
A water-soluble raw material was dissolved in 66.6% hot water with respect to the dry matter, and an emulsifier was dissolved in oil. Both were emulsified at 60 ° C., homogenized, and spray-dried to prepare a coffee whitener (powder type) containing tea polysaccharides derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2.

Formulation Example 30: Seasoning liquid for pickles containing an intestinal function improving agent Kelp extract 6 g
4g bonito extract
4g of salt
1 g of tea polysaccharide obtained in Production Example 2
The said component was melt | dissolved in ion-exchange water, the whole quantity was 100 g, and the seasoning liquid for pickles containing the tea polysaccharide derived from the green tea with a molecular weight of 400,000 or more obtained by manufacture example 2 was prepared.

Formulation example 31: Jelly containing intestinal function improving agent 1/5 Concentrated grape juice 10 g
20 g of tea polysaccharide obtained in Production Example 5
15g sugar
Glucose 20g
Minamata 12g
The above components were completely dissolved while heating with ion-exchanged water to make a total amount of 100 g. This was poured into a cup container, covered with a heat seal and cooled to prepare a jelly containing Oolong tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 5.

Formulation Example 32: Candy containing intestinal function improving agent Sugar 50 g
Minamata 100g
Citric acid 1g
Perfume 0.4g
Coloring agent 0.1g
50 g tea polysaccharide obtained in Production Example 6
Thirty candies containing tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 6 were prepared by a conventional method using the above-described components according to a candy formulation.

Formulation Example 33: Chewing gum containing an intestinal function improving agent Vinyl acetate resin 20 g
Plasticizer 3g
Polyisobutylene 3g
Microcrystal wax 2g
Calcium carbonate 2g
40g sugar
Glucose 20g
10 g of tea polysaccharide obtained in Production Example 2
Aspartame 0.3g
1g fragrance
Ingredients other than sugar and fragrance were placed in a pan, heated and dissolved, and mixed well. Cooled to 50 ° C., added and mixed with sugar, added fragrance at 40 ° C., molded, allowed to cool, and contains tea polysaccharide derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 A gum was prepared.

Formulation Example 34: Sweet chocolate bitter chocolate 33.2 g containing an intestinal function improving agent
50g sugar
10 g tea polysaccharide obtained in Production Example 8
Aspartame 0.31g
Cocoa butter 11.1g
The above-described components were mixed well at 40 ° C., and further kneaded for a long time with a reika machine to make the particles fine. It cooled after shaping | molding and the sweet chocolate containing the tea polysaccharide derived from the green tea with a molecular weight of 2 million or more obtained by manufacture example 8 was adjusted.

Formulation Example 35: Jam Orange peel containing intestinal function improving agent 250 g
100g sugar
Citric acid 0.5g
50 g of tea polysaccharide obtained in Production Example 2
The above-described components were boiled by a conventional method to prepare a jam containing tea polysaccharides derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2.

Formulation Example 36: Cookie containing intestinal function improving agent
74g starch
14g of water
15 g of tea polysaccharide obtained in Production Example 3
2 tsp baking powder
2 teaspoons of salt
1 egg butter 80g
2 tablespoons milk
Thirty cookies containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3 were prepared by a conventional method using the above-described cookie formulation.

Formulation Example 37: Custard cream containing intestinal function improving agent Upper white sugar 10 g
Glucose 5g
Minamata 6.2g
10 g of tea polysaccharide obtained in Production Example 2
3.8g of flour
Modified starch 7g
Nonfat dry milk 1.3g
Margarine 7.9g
Egg yolk (powder) 0.2g
Ion-exchanged water 41.9g
To a mixture of wheat flour, processed starch, egg yolk (powder) in a small amount of water, add the remaining water to 80 ° C and dissolve other ingredients, add it using a whisk and boil on high heat, A custard cream containing green tea-derived tea polysaccharides having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

Formulation Example 38: Kuriyokan Nangan 36.8g containing an intestinal function improving agent
Kanten 0.8g
7.2 g of water
40g sugar
10 g of tea polysaccharide obtained in Production Example 2
Water, sugar, and tea polysaccharides were added to the swelled water and dissolved by boiling under heating. After shaking this, it was boiled again, added with raw bean paste, and boiled until the total amount was 100 g. A kneaded candy containing green tea-derived tea polysaccharide having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

Formulation Example 39: Bread containing an intestinal function improving agent
3g dry yeast
Shortening 4g
Nonfat dry milk 5g
2g of salt
60g of water
10 g tea polysaccharide obtained in Production Example 3
The above ingredients were blended to prepare bread containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3 by a conventional method.

Formulation Example 40: Spaghetti flour containing intestinal function improving agent 100 g
25 g of water
5 g tea polysaccharide obtained in Production Example 3
Powders were mixed and kneaded uniformly while adding water little by little to prepare spaghetti containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 41: Chinese noodles containing an intestinal function improving agent Super strong powder 70 g
Potato starch 30g
1g of salt
Kansui 2g
48g of water
10 g tea polysaccharide obtained in Production Example 3
Mixing the raw materials described above, kneading, forming, compounding, aging and rolling according to conventional methods to form a noodle band, cut into noodle strings, and containing the insoluble tea polysaccharide derived from green tea obtained in Production Example 3 Chinese noodles were prepared.

Formulation Example 42: Instant Chinese noodles containing intestinal function improving agent Commercial Chinese noodle powder 60 g
Super strong powder 40g
Potato starch 12g
2g of salt
Commercial kansui 0.3g
35g of water
10 g tea polysaccharide obtained in Production Example 3
The raw materials described above were put into a mixer, mixed, rough rolled, rolled and cut out to obtain noodle strings. The noodle strings were steamed at 100 ° C. and fried in oil at 150 ° C. to prepare instant Chinese noodles containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 43: Frozen udon noodles containing an intestinal function improving agent 100 g
3g of salt
30 g of water
10 g tea polysaccharide obtained in Production Example 3
The components described above were put in a mixer, mixed, aged, rolled, and cut out to obtain noodle strings. The noodle strings were put in boiling water, boiled for about 5 minutes, and cooled to 10 ° C. with cold water. After taking out the noodle strings for one ball, draining them, putting them in a container with a uniform thickness, quickly freezing at −30 ° C., and insoluble tea derived from green tea obtained in Production Example 3 Frozen udon containing polysaccharides was prepared.

Formulation Example 44: Spring roll skin containing intestinal function improving agent Wheat starch 99 g
1g flour
Emulsifier 3g
2g salad oil
Sake 2g
2g of salt
43g of water
2 g tea polysaccharide obtained in Production Example 8
The ingredients described above were stirred and kneaded to make a pasty dough, applied to a rotating drum to a thickness of 1 mm and baked, and the resulting dough was cut into a cut 12 cm × 12 cm square. A spring roll peel containing tea polysaccharides derived from green tea having a molecular weight of 2 million or more was prepared.

Formulation Example 45: Beef & pork sausage containing an intestinal function improving agent 22g beef
17.3g of water
Salt 1.7g
0.01g salting agent
1g sugar
Sodium glutamate 0.5g
Spice 0.5g
Horse glue 3g
Pork shoulder meat 54g
10 g tea polysaccharide obtained in Production Example 3
The above ingredients were ground and mixed as it was and filled into a film. After being left as salted at 5 ° C. for 12 hours, boiled at 75 ° C. for 90 minutes and then refrigerated, to prepare beef and pork sausage containing insoluble tea polysaccharides derived from green tea obtained in Production Example 3.

Formulation Example 46: Kamaboko Surimi 60g containing an intestinal function improving agent
1.8g of salt
30.4g ice
6g starch
Seasoning 1.8g
6 g tea polysaccharide obtained in Production Example 3
The surimi, salt, and a small amount of ice were mixed, and break mixing was performed with a silent cutter for 5 minutes, and then the remaining ice and raw materials were added and mixed for 10 minutes. The mixture was finished when it became sticky at 15 ° C., molded, and fried with 160 ° C. oil for 4 minutes to prepare kamaboko containing the green tea-derived insoluble tea polysaccharide obtained in Production Example 3.

Formulation Example 47: Oral solution containing an intestinal function improving agent Tea polysaccharide obtained in Production Example 2 10 g
Carrot dry extract 214mg
Dried licorice extract 50mg
Ursodeoxycholic acid 25mg
D-sorbitol 50g
25g white sugar
Polyoxyethylene hydrogenated castor oil 3g
Propylene glycol 5mL
Sodium benzoate 600mg
Butyl paraoxybenzoate 10mg
Citrate buffer appropriate amount Ion-exchanged water was added to the above components to make the total amount 300 mL, and an internal liquid solution containing tea polysaccharide derived from green tea having a molecular weight of 400,000 or more obtained in Production Example 2 was prepared.

  As described above, the intestinal function improving agent of the present invention can be added to various oral application objects.

Claims (3)

An intestinal function improving agent comprising, as an active ingredient, a polysaccharide having a molecular weight of 400,000 or more obtained through a tea water or hot water extraction step .   A pharmaceutical composition for improving intestinal function, comprising an effective amount of the intestinal function improving agent according to claim 1.   A quasi drug for improving intestinal function, comprising an effective amount of the intestinal function improving agent according to claim 1.