JPS63165325A - Intestine-conditioning agent - Google Patents
Intestine-conditioning agentInfo
- Publication number
- JPS63165325A JPS63165325A JP61309240A JP30924086A JPS63165325A JP S63165325 A JPS63165325 A JP S63165325A JP 61309240 A JP61309240 A JP 61309240A JP 30924086 A JP30924086 A JP 30924086A JP S63165325 A JPS63165325 A JP S63165325A
- Authority
- JP
- Japan
- Prior art keywords
- water
- hemicellulose
- soluble
- intestine
- soluble polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000004676 glycans Chemical class 0.000 claims abstract description 21
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 21
- 239000005017 polysaccharide Substances 0.000 claims abstract description 21
- 229920002488 Hemicellulose Polymers 0.000 claims abstract description 11
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 8
- 235000009566 rice Nutrition 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 7
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 4
- 240000008042 Zea mays Species 0.000 claims abstract description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 3
- 235000005822 corn Nutrition 0.000 claims abstract description 3
- 239000010903 husk Substances 0.000 claims abstract description 3
- 240000007594 Oryza sativa Species 0.000 claims abstract 2
- 230000000968 intestinal effect Effects 0.000 claims description 21
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 239000004480 active ingredient Substances 0.000 claims description 6
- 241000186000 Bifidobacterium Species 0.000 abstract description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 6
- 239000000843 powder Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical group N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 3
- 229910001873 dinitrogen Inorganic materials 0.000 abstract description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 abstract description 3
- 230000035755 proliferation Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract description 2
- 239000006227 byproduct Substances 0.000 abstract description 2
- 235000013339 cereals Nutrition 0.000 abstract description 2
- 210000000936 intestine Anatomy 0.000 abstract description 2
- 239000003456 ion exchange resin Substances 0.000 abstract description 2
- 229920003303 ion-exchange polymer Polymers 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 230000009967 tasteless effect Effects 0.000 abstract description 2
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 238000005498 polishing Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 241000209094 Oryza Species 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 235000013325 dietary fiber Nutrition 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 210000004534 cecum Anatomy 0.000 description 3
- 239000008213 purified water Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 241000894007 species Species 0.000 description 2
- OMDQUFIYNPYJFM-XKDAHURESA-N (2r,3r,4s,5r,6s)-2-(hydroxymethyl)-6-[[(2r,3s,4r,5s,6r)-4,5,6-trihydroxy-3-[(2s,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]methoxy]oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@H](O)[C@H](O)O1 OMDQUFIYNPYJFM-XKDAHURESA-N 0.000 description 1
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 229920000926 Galactomannan Polymers 0.000 description 1
- 229920002581 Glucomannan Polymers 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 239000004368 Modified starch Substances 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 229920000715 Mucilage Polymers 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000109329 Rosa xanthina Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000020940 control diet Nutrition 0.000 description 1
- 229960002433 cysteine Drugs 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 230000003636 fecal output Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 229940046240 glucomannan Drugs 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
【発明の詳細な説明】
皮栗上鬼■里分立
本発明は、水溶性多１）！類を有効成分とする整腸剤に
関する。本発明に係る水溶性多糖類は、腸内細菌として
有益なビフィドバクテリウム菌に資化されるため、ビフ
ィドバクテリウム菌の腸内増殖を促進して整腸作用をす
るものと考える。[Detailed Description of the Invention] The present invention is based on water-soluble polyester 1)! The present invention relates to an intestinal regulating agent containing the following as an active ingredient. The water-soluble polysaccharide according to the present invention is assimilated by Bifidobacterium, which is useful as an intestinal bacterium, and is therefore considered to promote intestinal proliferation of Bifidobacterium and have an intestinal regulating effect.
及止負皇員
セルロース、ヘミセルロース、水溶性及び不溶性ペクチ
ン質、リグニン、キチン、粘質物（ガラクトマンナン、
グルコマンナンなど）、海藻多糖質、化学修飾多糖ｍｌ
（化工澱粉、カルボキシメチルセルロース）等は、食物
繊維（ダイエタリー・ファイバー）又は食餌性繊維と称
せられ、ヒトの消化酵素により消化されない食物中の難
消化性成分であるが、近年、それの摂取が有益な生理作
用を示すことから注目されてきている。Cellulose, hemicellulose, water-soluble and insoluble pectin, lignin, chitin, mucilage (galactomannan,
glucomannan, etc.), seaweed polysaccharide, chemically modified polysaccharide ml
(modified starch, carboxymethylcellulose), etc. are called dietary fibers or dietary fibers, and are indigestible components of food that are not digested by human digestive enzymes, but in recent years, their intake has become beneficial. It has attracted attention because of its physiological effects.
すなわち、これらの食物繊維を摂取すると、■腸の活動
を高め、食物の腸内通過時間を短縮して有害物質の吸収
を阻害する、■腸内容量及び糞便量を増大する、■コレ
ステロール、胆汁酸、重金属を吸着して排泄する、■腸
内菌のバランスを有用菌優位として無害化する、等の生
理上の効果があると報告されている。In other words, ingesting these dietary fibers can: 1) increase intestinal activity, shorten the intestinal transit time of food and inhibit the absorption of harmful substances, 2) increase intestinal capacity and fecal volume, 2) reduce cholesterol and bile It has been reported that it has physiological effects such as adsorbing and excreting acids and heavy metals, and making the balance of intestinal bacteria in favor of beneficial bacteria, making them harmless.
一方、ビフィドバクテリウム菌はヒトの腸管内に生育し
、腸内のｐｎを低下させることにより、腐敗性細菌の増
殖に対して拮抗的作用を示して腐敗生成物の生成を抑制
する等生理的に有用な菌種であることが知られている。On the other hand, Bifidobacterium grows in the human intestinal tract, and by lowering the PN in the intestines, it exhibits an antagonistic effect on the growth of putrefactive bacteria and inhibits the production of putrefactive products. It is known to be a useful bacterial species.
本発明者は、上記食物繊維に含まれる水溶性多＃Ｐ！１
の腸内細菌に対する資化性を検討した結果、水溶性多糖
類はビフィドバクテリウム菌に選択的に資化され、一方
腐敗性細菌に資化されないことを見出し、本発明をなす
に至った。The present inventor has discovered that the water-soluble poly#P contained in the above-mentioned dietary fiber! 1
As a result of examining the assimilation ability of water-soluble polysaccharides to intestinal bacteria, it was discovered that water-soluble polysaccharides are selectively assimilated by Bifidobacterium bacteria, but not by putrefactive bacteria, leading to the present invention. .
明が解ンしようとする諜
本発明は、腸内有用菌であるビフィドバクテリウム閑に
選択的に資化される水溶性多糖類を有効成分とする整腸
剤を提供することを課題とする。The purpose of the present invention is to provide an intestinal regulating agent containing as an active ingredient a water-soluble polysaccharide that is selectively assimilated by Bifidobacterium, a beneficial intestinal bacterium.
以下本発明の詳細な説明する。The present invention will be explained in detail below.
又皿且盪底
本発明の特徴は、上記水溶性多糖類を有効成分とする整
腸剤にある。すなわち、水溶性多糖類の摂取により腸内
細菌であるビフィドバクテリウム閏の増殖を促進するこ
とにより、該多Ｉ！類自体の生理的作用とビフィドバク
テリウム菌の生理的作用により整腸効果を示すものであ
る。Another feature of the present invention is an intestinal regulating agent containing the above-mentioned water-soluble polysaccharide as an active ingredient. That is, by promoting the growth of intestinal bacteria, Bifidobacterium, by ingesting water-soluble polysaccharides, the polyI! It shows an intestinal regulation effect due to the physiological action of the species itself and the physiological action of Bifidobacterium bacteria.
ｉ を” するための
本発明で有効成分として利用する水溶性多糖類は、米糠
、小麦麩またはトウモロコシ外皮等の穀物精選に際して
得られる副生物を１−へキサンのような有機溶媒で脱脂
処理した後、水酸化ナトリウム溶液を加えて窒素ガスで
置換した容器内で抽出することにより得られる３この抽
出液から遠心分離等の操作により残渣を除去し、中和し
た後、イオン交換樹脂により除蛋白した上清に、適宜脱
塩処理を行った後、エタノールを加えるとヘミセルロー
ス（Ｂ）の沈澱物が得られ、この沈澱物を適宜乾燥する
とヘミセルロース（Ｂ）の粉末が得うれる。The water-soluble polysaccharide used as an active ingredient in the present invention for "i" is obtained by degreasing by-products obtained during grain sorting such as rice bran, wheat bran, or corn husk with an organic solvent such as 1-hexane. After that, a sodium hydroxide solution is added and extracted in a container purged with nitrogen gas.Residues are removed from this extract by operations such as centrifugation, neutralized, and protein is removed using an ion exchange resin. After appropriately desalting the supernatant, ethanol is added to obtain a hemicellulose (B) precipitate, and this precipitate is appropriately dried to obtain a hemicellulose (B) powder.
このようにして得られるヘミセルロース（Ｂ）粉末は、
無味であって、水溶性であるため、タブレット形態、ド
リンク剤形態として摂取することができ、また、そのま
ま食物に混合して摂取してもよい。The hemicellulose (B) powder obtained in this way is
Since it is tasteless and water-soluble, it can be taken in the form of tablets or drinks, or it can be mixed with food and taken as is.
なお、このヘミセルロースはアラビノースとキシロース
が結合した形態の多糖である。次に、参考として脱脂米
糠及び小麦麩由来の水溶性多糖類の成分組成を表１に示
す。Note that this hemicellulose is a polysaccharide in which arabinose and xylose are combined. Next, as a reference, the component compositions of water-soluble polysaccharides derived from defatted rice bran and wheat gluten are shown in Table 1.
次に、上記ヘミセルロース（Ｂ）粉末の腸内細菌に対す
る資化性試験を行った結果を示す。Next, the results of an assimilation test on the intestinal bacteria of the hemicellulose (B) powder will be shown.
に・する ヒ 試
試験方法：
ヒト由来のストレプトコツカス属、ラクトバチルス属、
ビフィドバクテリウム属、クロストリジウム属、スタフ
ィロコッカス属、及びバタテロイデス属に属する菌株を
用いて行った。Test method: Human-derived Streptococcus, Lactobacillus,
The test was carried out using strains belonging to the genera Bifidobacterium, Clostridium, Staphylococcus, and Batatelloides.
培地は、バタテロイデス属にＰＹＦ液体培地を用い、そ
れぞれに水溶性多糖類０．２５％を無菌的に添加して用
いた。この培地に各菌体懸濁液を菌数が１０’〜１０’
個となるように接種し、嫌気的に４８時間培養した。一
方、対照としてグルコース０．５％添加培地、また盲検
として糖類無添加培地を用い、同様に各菌体を培養した
。As a medium, a PYF liquid medium was used for Batasteroides, and 0.25% of water-soluble polysaccharide was added aseptically to each medium. Add each bacterial suspension to this medium until the number of bacteria is 10' to 10'.
The cells were inoculated into individual cells and cultured anaerobically for 48 hours. On the other hand, each bacterial cell was similarly cultured using a medium supplemented with 0.5% glucose as a control and a medium without sugar added as a blind test.
各菌体の資化性は、グルコース培養液の６６０ｎｍにお
ける吸光度の値がら盲検値を差し引いた値を１００とし
、各培養液の６６０ｎｍにおける吸光度の値から盲検値
を差し引いた値を換算して表した。The assimilation ability of each bacterial cell is calculated by subtracting the blinded value from the absorbance value at 660 nm of the glucose culture solution, and setting it as 100. It was expressed as follows.
表２
ＰＹＦ液体培地（Ｐｅｐｔｏｎｅ ｙｅａｓｔ ｅｘｔ
ｒａｃｔ Ｆｉｌｄｅｓｓｏｌｕｔｉｏｎ ｂｒｏｔｈ
）の組成Ｔｒｙｐｔｉｃａｓｅ （ＢＢＬ社製）１
０ｇ’１ｅａｓｔ ｅｘｔｒａｃｔ （Ｄｉｆｃｏ社製
） ５ｇＦｉｌｄｅｓ ５ｏｌｕｔｉｏｎ （注
１） ４０ ｍ１Ｓａｌｔｓ ５ｏｌｕｔｉ
ｏｎ （注２） ４０ ｖｇｌＬ−Ｃｙｓｔ
ｅｉｎｅ ＨＣｌ−Ｈ００，５ｇ精製水
９２０ ｅｓｌｐＨ７，２に調整。Table 2 PYF liquid medium (Peptone yeast ext.
ract Fildessolution broth
) Composition of Trypticase (manufactured by BBL) 1
0g'1east extract (manufactured by Difco) 5gFildes 5olution (Note 1) 40 m1Salts 5oluti
on (Note 2) 40 vglL-Cyst
eine HCl-H00, 5g purified water
920 esl Adjust pH to 7.2.
（注１） Ｆｉｌｄｅｓ ５ｏｌｕｔｉｏｎ生理的食塩
水 １５０ ｔａｌｌ濃塩酸
６−ｌ
馬脱繊血 ５０−１
ペプシン（１：１０，０００． Ｄｉｆｃｏ社製）１ｇ
５５℃で一夜酵素反応した薇、２０％ＮａＯＨでｐｉ
７．６に調整。(Note 1) Fildes 5 solution physiological saline 150 tall concentrated hydrochloric acid
6-l Horse defibrinated blood 50-1 Pepsin (1:10,000. Manufactured by Difco) 1 g
Roses enzymatically reacted overnight at 55°C, pi with 20% NaOH
Adjusted to 7.6.
（注２） ５ａｌｔｓ ５ｏｌｕｔｉｏｎＣａＣ１ｚ
０．２ｇ、 Ｍｇ５Ｏａ ０．２ｇ１ＫｇＨＰＯ＊
Ｉｇ 。(Note 2) 5alts 5solutionCaC1z
0.2g, Mg5Oa 0.2g1KgHPO*
Ig.
ＫＨ！ＰＯ４１ｇ ５ＮａＨＣＯｓ １０ｇ％ＮａＣ１
２ｇを１．０００蒙ｌの 精製水に溶解。KH! PO41g 5NaHCOs 10g%NaCl
Dissolve 2g in 1.000ml of purified water.
表３
ＬＢ液体培地の組成
８ａｃｔｏ−Ｌｆｖｅｒ （旧ｆｃｏ社製）滲出液 １
，０００ｍ／Ｐｒｏｔｅｏｓｅ ｐｅｐｔｏｎｅ Ｎ
ａ ３（Ｄｉｆｃｏ社製） １０ ｇＴｒｙｐｔｉ
ｃａｓｅ （ＢＢＬ社製） ５ｇＹ
ｅａｓｔ ｅｘｔｒａｃｔ （Ｄｉｆｃｏ社製）
３ｇＴｗｅｅｎ８０
１ ｇＳｏｌｕｔｉｏｎ Ｂ （注１）
５ｍ１ｌＬ−Ｃｙｓｔｅｉｎｅ−ＩＣＩ
０．２ｇｐＨ７，２に調整。Table 3 Composition of LB liquid medium 8acto-Lfver (formerly manufactured by FCO) Exudate 1
,000m/Proteose peptone N
a 3 (manufactured by Difco) 10 gTrypti
case (manufactured by BBL) 5gY
east extract (manufactured by Difco)
3gTween80
1 gSolution B (Note 1)
5mlL-Cysteine-ICI
0.2g Adjusted to pH 7.2.
（注１） ５ｏｌｕｔｉｏｎ Ｂ
ＭｇＳＯ（７１）ｚ０１０ｇ、 Ｆ１３ＳＯ４’７１）
！ＯＯ，５ｇ 、 ＮａＣ１Ｏ，５ｇ、Ｍｎ５Ｏ＜ ０
．３３７ｇを２５０ｍ ｊ！の精製水に溶解。(Note 1) 5olution B MgSO(71)z010g, F13SO4'71)
! OO, 5g, NaC1O, 5g, Mn5O< 0
．． 337g for 250mj! Dissolved in purified water.
試験結果： 表４に示すとおりである。Test results: As shown in Table 4.
上記試験結果から、本発明の有効成分である水溶性多ｔ
ｉｅがビフィドバクテリウム菌に選択的に資化されるこ
とがわかる。From the above test results, it was found that the water-soluble polytamine, which is the active ingredient of the present invention,
It can be seen that ie is selectively utilized by Bifidobacterium.
以下実施例を示して本発明とその効果を具体的に説明す
る。EXAMPLES The present invention and its effects will be specifically explained below with reference to Examples.
実施例
水溶性多糖類の調製：
脱脂した米糠１００ｇに０．５Ｎ水酸化ナトリウム溶液
１）を加え、窒素ガスで置換した容器内で１）３０スト
ロ一ク／分、１８時間で抽出した。この抽出液を遠心分
離（３，ＯＯＯｒｐｍ、 １０分間）で残渣を除去し、
酢酸で中和した後、最終濃度７％になるようトリクロル
酢酸を加えて除蛋白して上滑を得た。Example Preparation of water-soluble polysaccharide: 0.5N sodium hydroxide solution 1) was added to 100 g of defatted rice bran, and extracted in a container purged with nitrogen gas at 1) 30 strokes/min for 18 hours. This extract was centrifuged (3,000 rpm, 10 minutes) to remove the residue.
After neutralizing with acetic acid, trichloroacetic acid was added to a final concentration of 7% to remove protein and obtain a supernatant.
次に、限外濾過で脱塩し、上清の約４倍量のエタノール
を加えて水溶性多糖の沈澱を得た。Next, the mixture was desalted by ultrafiltration, and about 4 times the amount of ethanol as the supernatant was added to obtain a precipitate of water-soluble polysaccharide.
この沈澱物を水で溶解した後、凍結乾燥してヘミセルロ
ース（Ｂ）４ｇの粉末を得た。This precipitate was dissolved in water and then freeze-dried to obtain 4 g of hemicellulose (B) powder.
なお、上記脱脂米糠に代えて小麦麩１００ｇを用いて同
様に処理した場合にはヘミセルロース（Ｂ）６ｇの粉末
が得られる。In addition, when 100 g of wheat bran is used in place of the defatted rice bran and the same treatment is performed, 6 g of hemicellulose (B) powder is obtained.
水溶性多糖の整腸作用：
次に、上述のごとくして得られた米糠由来の多糖類の整
腸作用を調べるために、下記により動物試験を行った。Intestinal regulation effect of water-soluble polysaccharide: Next, in order to investigate the intestinal regulation effect of the rice bran-derived polysaccharide obtained as described above, the following animal test was conducted.
盲腸的内容物のｐＨ測定
体重約７０ｇのＳＤ系ラット（日本タレア■製）を対照
飼料で７日間予備飼育した後、１群４匹ずつ２群に分け
、表５に示したごとくの実験飼料を投与して、３週間飼
育した。なお、飼料及び水は自由に摂取させた。Measurement of pH of cecal contents SD rats (manufactured by Nippon Talea ■) weighing approximately 70 g were preliminarily fed with a control diet for 7 days, and then divided into two groups of 4 rats each, and fed with experimental diets as shown in Table 5. was administered and reared for 3 weeks. In addition, feed and water were available ad libitum.
盲腸的内容物のｐＨは、エーテル麻酔下で開腹して盲腸
的内容物を取出し、ｐｌ＋メーターにより測定した。The pH of the cecal contents was measured using a pl+ meter after the abdomen was opened under ether anesthesia and the cecal contents were removed.
動物実験に用いた飼料の成分組成を表５に飼料投与後の
盲腸的内容物のｐｎを表６に腸内菌代謝産物の有機酸量
を表７にそれぞれ示す。The composition of the feed used in the animal experiment is shown in Table 5, the pn of the cecal contents after administration of the feed is shown in Table 6, and the amount of organic acid in intestinal bacteria metabolites is shown in Table 7.
表 ５
動物実験に用いた飼料の成分組成（％）表 ６
飼料投与後の盲腸的内容物のｐＨ（ＭｅａｎｔｈＳ、Ｄ
、）表７
盲腸内の有機酸量（ＬＩＩｇ／盲腸）
表６に示す通り、米糠由来の多ＩＪ！類を投与すること
により盲腸内のｐＨが低下し、又表７に示す通り、ビフ
ィドバクテリウム菌の代謝産物である酢酸が産生され、
従って腐敗性細菌の繁殖を抑制することが期待される。Table 5 Component composition (%) of feed used in animal experiments Table 6 pH of cecal contents after feed administration (MeanthS, D
) Table 7 Amount of organic acids in the cecum (LIIg/cecum) As shown in Table 6, many IJs derived from rice bran! By administering Bifidobacterium, the pH in the cecum decreases, and as shown in Table 7, acetic acid, which is a metabolic product of Bifidobacterium, is produced.
Therefore, it is expected to suppress the proliferation of spoilage bacteria.
Claims (3)
請求の範囲第（１）項記載の整腸剤。(2) The intestinal regulating agent according to claim (1), wherein the water-soluble polysaccharide is hemicellulose (B).
皮由来である特許請求の範囲第（１）項又は第（２）項
記載の整腸剤。(3) The intestinal regulating agent according to claim (1) or (2), wherein the water-soluble polysaccharide is derived from rice bran, wheat bran, or corn husk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61309240A JPS63165325A (en) | 1986-12-27 | 1986-12-27 | Intestine-conditioning agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61309240A JPS63165325A (en) | 1986-12-27 | 1986-12-27 | Intestine-conditioning agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63165325A true JPS63165325A (en) | 1988-07-08 |
| JPH045649B2 JPH045649B2 (en) | 1992-02-03 |
Family
ID=17990614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61309240A Granted JPS63165325A (en) | 1986-12-27 | 1986-12-27 | Intestine-conditioning agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63165325A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02276801A (en) * | 1988-12-07 | 1990-11-13 | Snow Brand Milk Prod Co Ltd | Water-soluble hemicellulose, its production and health food containing the same |
| JPH03209331A (en) * | 1990-01-10 | 1991-09-12 | Nippon Shokuhin Kako Co Ltd | Substance for improving intestinal environment |
| JPH05112455A (en) * | 1991-06-17 | 1993-05-07 | Snow Brand Milk Prod Co Ltd | Colon cancer suppressing substance |
| JP2001322942A (en) * | 2000-05-15 | 2001-11-20 | Nippon Shokuhin Kako Co Ltd | Immune activator |
| JP2005179279A (en) * | 2003-12-19 | 2005-07-07 | Mitsui Norin Co Ltd | Intestinal function ameliorator |
| JP2007222126A (en) * | 2006-02-27 | 2007-09-06 | Chikuno Shokuhin Kogyo Kk | Candy using deproteinized and defatted rice bran |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51142546A (en) * | 1975-04-09 | 1976-12-08 | Draco Ab | Intestines adjusting agent |
| JPS62205766A (en) * | 1986-03-04 | 1987-09-10 | Tanpei Seiyaku Kk | Fibrous food |
-
1986
- 1986-12-27 JP JP61309240A patent/JPS63165325A/en active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS51142546A (en) * | 1975-04-09 | 1976-12-08 | Draco Ab | Intestines adjusting agent |
| JPS62205766A (en) * | 1986-03-04 | 1987-09-10 | Tanpei Seiyaku Kk | Fibrous food |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02276801A (en) * | 1988-12-07 | 1990-11-13 | Snow Brand Milk Prod Co Ltd | Water-soluble hemicellulose, its production and health food containing the same |
| JPH03209331A (en) * | 1990-01-10 | 1991-09-12 | Nippon Shokuhin Kako Co Ltd | Substance for improving intestinal environment |
| JPH05112455A (en) * | 1991-06-17 | 1993-05-07 | Snow Brand Milk Prod Co Ltd | Colon cancer suppressing substance |
| JP2001322942A (en) * | 2000-05-15 | 2001-11-20 | Nippon Shokuhin Kako Co Ltd | Immune activator |
| JP4698796B2 (en) * | 2000-05-15 | 2011-06-08 | 日本食品化工株式会社 | Immunostimulator |
| JP2005179279A (en) * | 2003-12-19 | 2005-07-07 | Mitsui Norin Co Ltd | Intestinal function ameliorator |
| JP2007222126A (en) * | 2006-02-27 | 2007-09-06 | Chikuno Shokuhin Kogyo Kk | Candy using deproteinized and defatted rice bran |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH045649B2 (en) | 1992-02-03 |
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Legal Events
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|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |