JPH03285653A - Fat metabolism improving substance and production thereof - Google Patents

Abstract

PURPOSE:To produce a fat metabolism improving substance of a specific component consisting essentially of beta-glucan by removing protein from an extracted solution with an alkaline aqueous solution from grain, desalting and drying. CONSTITUTION:The whole grain flour or polished flour of preferably oat or barley is extracted with an alkali aqueous solution under heating. The residue is removed from the extracted solution, adjusted with an aqueous solution of an inorganic aid or organic acid to proper pH to precipitate protein. The protein is removed from the solution by centrifugal separation, etc., and the residue is concentrated. Then an alcohol is added to the concentrated solution and prepared precipitate is collected. The precipitate is washed preferably with ethanol several times, dried and ground to give a gum substance of grain effective for improving fat metabolism consisting essentially of beta-glucan. The concentrated solution is desalted by ultrafiltration having tens of thousands fractionated molecular weight, electrophoresis or ion exchange resin, dried and ground to produce the gum substance of grain.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Topics] Saekatsu The present invention relates to a novel gum quality whose main component is β-glucan extracted and purified from grains such as oats and barley, and a method for producing the same.

Furthermore, the present invention relates to a lipid metabolism improving product containing such a novel gum substance as an active ingredient.

Tech'i Drunken Punishment Fierce Dietary Fiber is 1. Indigestible components in food that are not digested by human digestive enzymes, including cellulose, hemicellulose, lignin, water-soluble and water-insoluble betatin, mucilage (β-glucan, galac). These include naan, glucofunna, 1 etc.), seaweed polysaccharides, ＼-L starch, carboquine methyl cellulose, etc. Is dietary fiber water due to its nature? Water-soluble dietary fiber is broadly classified into 8 types and water-insoluble.Water-soluble dietary fiber (1) inhibits the absorption of harmful substances or carcinogenic substances present in the intestines, and tJP? Do tQ. (2) Adsorbs and excretes cholesterol, bile acids, and heavy metals. (3) It has been recognized that it has physiological effects such as improving the intestinal environment by making intestinal bacteria predominant as useful bacteria. on the other hand,
Water-insoluble dietary fiber has been recognized to have physiological effects such as (1) increasing intestinal spiral movement and shortening the time taken for food to pass through the intestines, and (2) increasing intestinal circulation and fecal volume. .

In addition, grains, particularly oats and barley, are not only rich in protein, fat, and minerals, but also contain a large amount of dietary fiber. Oats and barley contain a good balance of water-soluble dietary fiber and water-insoluble dietary fiber, and the water-soluble dietary fiber is a grain gum whose main component is β-glucan.

The inventors of the present invention have conducted extensive research into what the grain gum is, and have established a method for specifically collecting the grain gum. We have elucidated the problem of grain gum G and further discovered that by ingesting grain gum, it is possible to improve the symptoms of lipid metabolism abnormalities, especially genetic lipid metabolism abnormalities, and have completed the present invention. It is.

That is, an object of the present invention is to provide a grain gum containing β-glucan obtained from grains, particularly oats and barley, as a main component, and a method for producing the same.

Furthermore, an object of the present invention is to provide a lipid metabolism improving product containing such grain gum as an active ingredient.

The present invention has been made to achieve the above-mentioned problems, and uses grain as a raw material, extracts it with an alkaline aqueous solution, and makes the extract acidic. The main component is β-glucan, which can be obtained by precipitating and removing proteins, then adding alcohol to the residual solution to precipitate it, or desalting it by ultrafiltration, electrodialysis, or ion exchange resin, and then drying it. This article relates to grain gum and its manufacturing method.

Grains used as raw materials in the present invention include wheat, barley, oats, oats, barley such as rye, rice, millet, and millet, but barley and oats are particularly rich in dietary fiber. Moreover, it is desirable as a raw material because it contains water-soluble dietary fiber and water-insoluble dietary fiber in a well-balanced manner.

It is best to use whole grain or refined oats or barley. Extraction of the raw materials for Gorera using alkaline water solubility involves adding water to the raw materials, adding an alkaline aqueous solution to the raw materials to make the raw materials alkaline, and stirring and extracting under heating.

As the alkali, caustic soda, caustic potash, sulfur/lium carbonate, etc. are used, and the extract is made alkaline to about 119 to 11, heated to around 40 to 50°C with stirring, and extracted for about several tens of minutes to an hour. do. In this way, the grain gum of the present invention is extracted and transferred into the extract.

This extract is separated into an extract and a residue by filtration or centrifugation, and the extract is collected.

Next, the extract is acidified to remove proteins by precipitation, and the remaining liquid is collected.

To make the extract acidic, add an aqueous solution of an inorganic acid such as hydrochloric acid or sulfuric acid or an organic acid such as citric acid to the extract to bring the pH of the extract close to pH 4.5, which is the isoelectric point of the protein. Do this by adjusting. In this way, proteins are precipitated and removed.

A filtrate or supernatant is collected by filtering or centrifuging this extract.

These residual liquids are concentrated to about a fraction of their volume.

Usually, this concentration is carried out by vacuum concentration or ultrafiltration.

Then, alcohol is added to this concentrated solution to form a precipitate, and this precipitate is collected. As the alcohol, alcohols such as isopropanol and ethanol are used, but it is preferable to use ethanol from the viewpoint of food hygiene.

The resulting precipitate is a grain gum containing β-glucan as a main component, and this is washed several times with alcohol, especially ethanol, dried and ground to obtain a grain gum. Alternatively, the concentrated solution may be subjected to ultrafiltration with a molecular weight cut-off of tens of thousands, electrodialysis,
After desalting using any of the ion exchange resins, the grains are dried and crushed to obtain the above-mentioned grain gum.

Furthermore, the present invention relates to a lipid metabolism improving product containing the grain gum thus obtained as an active ingredient.

In the present invention, the effect of improving lipid metabolism abnormalities was investigated using the above-mentioned grain gum in genetically obese runts as test animals. As a result, it was found that it has a hypercholesterolemia-improving effect, a fatty liver suppressing effect, and can improve lipid metabolic abnormalities, as described below.

In the present invention, lipid metabolic abnormalities can be improved not by administering a drug, but by incorporating a small amount of the drug into a meal.

The blending amount in the present invention is preferably about 1 to 5 g of the grain gummy powder per adult per day. This is preferably administered orally by adding it to a meal, such as cooking rice or miso soup, or adding it to a soup. It may also be administered by incorporating it into candy or the like, or by mixing it with lactose or the like and forming it into a tablet.

The present invention will be specifically described with reference to Examples.

Implementation ■ Whole grain oat flour and refined barley flour (refined yield 73%)
was used as the raw material. The compositions of these raw materials are shown in Table 1.

Analyzed by Asp method.

Grain gum quality samples were prepared from these raw materials according to the following procedure. Add water at 30°C to 6 kg of each raw material, add a 20% solution of sodium carbonate, and adjust to ρ1110.
Extraction was carried out with stirring at 45°C for 30 minutes.

A decanter that uses centrifugal force at 6000 rpm to collect the extract.
The residue was extracted twice using the same method 0, and the extract was collected.

Next, 2M hydrochloric acid was added to the extract to adjust the pH to 4.5 to precipitate the protein fraction, followed by solid-liquid separation in a continuous centrifuge using 17,000 G centrifugal force, and the supernatant was collected. did. This supernatant was concentrated under reduced pressure using a rotary elevator to a volume of 115, and then 4 times the amount of ethanol was added to precipitate a polysaccharide fraction containing grain gum as the main component. Furthermore, the polysaccharide fraction was added to ethanol 10I! The grains were washed, dried with ventilation, and crushed to obtain a grain gum quality specimen. 200g each of recovered oat and barley gum quality samples
, 180g.

The composition of these grain gums is shown in Table 2.

Table 2 Grain gum component composition (Dry matter%) *Analyzed using the Asp method.

Next, the effects of each grain gum on improving lipid metabolism abnormalities were investigated using genetically obese rats as test animals.

Using genetically obese Zucker rats and normal rats (both male, withdrawn from membership at age 6) as experimental animals, oat and barley grain gum quality samples were added to the feed to prepare feed with the composition shown in Table 3. did. Each experimental diet was administered to genetically obese rats in groups of 8 for 34 days. In addition, standard feed was administered to 7 normal rats for the same period.

In addition, feed and water were available ad libitum.

Table 3 Feed Composition (%) After administering the above experimental feed, blood was collected from the tail vein after a 7-hour fast on the 7th day, 14th day, 21st day, and 28th day, and serum was separated. Serum cholesterol levels were measured by an enzymatic method using Decuminar TC-5Cfa (manufactured by Wamedex Co., Ltd.). After a 17-hour fasting period after administration of the feed, the animals were dissected under ether anesthesia and serum lipids were measured in the same manner.

The results are shown in FIG.

Genetically obese rats (-〇-) fed standard feed had significantly increased cholesterol levels and hypercholesterolemia compared to normal rats (-.-). On the other hand, in genetically obese rats, cholesterol levels on oats (-△-) and barley gum diets (-low-) were significantly lower than those on standard diets, with a significant difference of 1% using stochastic processing. showed a significantly lower value with a small risk ratio.

From the above results, it is judged that this substance has a hypercholesterolemia improving effect.

Furthermore, an autopsy is performed, the liver and adipose tissue are removed, and the weight is measured.The lipids in the liver are extracted, the cholesterol level is measured using the above method, and the triglyceride level is measured using the Triglyceride Test Wako (manufactured by Wako Pure Chemical Industries, Ltd.). It was measured by the acetylacetone color method using .

The results are shown in Table 4.

5 6 Genetically obese rats administered the standard diet exhibited weight gain, overeating, liver enlargement (fatty liver), and body fat accumulation (obesity) compared to normal rats. On the other hand, in genetically obese rats, as shown in Table 4, the oat and barley gummy diets had lower liver weights and liver cholesterol and triglyceride levels by 50 to 50% compared to the standard diets.
It showed a low value of 60%, and a significant difference test using stochastic processing revealed a significant difference with a very small risk rate of 1%.

From the above results, it was determined that the cereal gum of the present invention has a strong fatty liver suppressing effect.

100 g each of the oat gum powder and the barley gum powder obtained in Example 1 were mixed with 1 g of sodium β-glutamate to prepare a food additive for improving lipid metabolism.

Approximately 2% of this food additive is added to cooked rice during cooking.

Since the grain gum of the present invention has a lipid metabolism improving effect, it can be used as a lipid metabolism improving food additive containing it as an active ingredient. In addition, this food additive does not suddenly alleviate lipid metabolism by administering a drug, but rather
It can be gradually relieved by adding a small amount to your meals.

Furthermore, according to the production method of the present invention, the grain gum can be ingested efficiently.

[Brief explanation of drawings]

Figure 1 shows changes in serum cholesterol levels when normal rats and genetically obese rats shown in the test example were fed standard feed and grain-gum supplemented feed (-・- indicates the standard feed group for normal rats; -〇 indicates a standard diet for genetically obese rats. -△ indicates an oat/gum diet for genetically obese rats, and row indicates a barley/gum diet for genetically obese rats).

Claims (5)

[Claims] (1) Extract grains with an alkaline aqueous solution, acidify the extract to remove proteins by precipitation, and then add alcohol to the remaining solution to precipitate or desalt and dry to obtain β-glucan. Cereal gum having the following properties as ingredients [1] Weight average molecular weight: 100,000 to 1,000,000 [2] Sugar composition: Glucose 70% by weight or more (β-glucan 60% by weight or more) Arabinose 2-15% by weight Xylose 2 ~15% by weight Mannose, galactose, uronic acid (trace amounts) [3] Solubility: Soluble in water, alkali, and acid aqueous solutions. Acetone, benzene, alcohol, hydroalcohol,
Insoluble in chloroform. [4] Basic, acidic, neutral: Neutral [5] Properties: White to light brown. Tasteless, odorless (2) Claim (1) in which the grain is one or more types selected from the group consisting of wheat, that is, oats, barley, etc.
) grain gum quality (3) Lipid metabolism improving product containing the grain gum of claim (1) or (2) as an active ingredient (4) The lipid metabolism improving product according to claim (3), which improves the symptoms of genetically expressed lipid metabolism abnormality. (5) After extracting the grains with an alkaline aqueous solution and removing proteins by deproteinizing the resulting extract, adding alcohol to the remaining liquid for precipitation, or ultrafiltration with a molecular weight cut-off of tens of thousands; β-, which is characterized by being desalted by either electrodialysis or ion exchange resin and then dried and collected.
Method for producing grain gum having the following properties mainly composed of glucan [1] Weight average molecular weight: 100,000 to 1,000,000 [2] Sugar composition: Glucose 70% by weight or more (β-glucan 60% by weight or more) Arabinose 2 ~15% by weight Xylose 2~15% by weight Mannose, galactose, uronic acid (trace amounts) [3] Solubility: Soluble in water, alkali, and acid aqueous solutions. Acetone, benzene, alcohol, hydroalcohol,
Insoluble in chloroform. [4] Basic, acidic, neutral: Neutral [5] Properties: White to light brown. Tasteless, odorless