CN112691115A - Arabinoxylan and compound for preventing/treating gastrointestinal mucosa and liver injury - Google Patents
Arabinoxylan and compound for preventing/treating gastrointestinal mucosa and liver injury Download PDFInfo
- Publication number
- CN112691115A CN112691115A CN202110135233.2A CN202110135233A CN112691115A CN 112691115 A CN112691115 A CN 112691115A CN 202110135233 A CN202110135233 A CN 202110135233A CN 112691115 A CN112691115 A CN 112691115A
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- arabinoxylan
- solution
- food
- use according
- konjac flour
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses application of arabinoxylan or a compound of the arabinoxylan and konjac flour in preparing food, health-care products or medicines for preventing/treating alcoholic gastrointestinal mucosa and liver injury. The arabinoxylan is prepared by pretreating plant raw materials to remove impurities, and then leaching with alkali liquor, dealkalizing and purifying. In the compound of the arabinoxylan and the konjac flour, the mass percentage of the arabinoxylan is 1-99%. The food, the health care product or the medicine for preventing/treating the alcoholic gastrointestinal mucosa and liver injury, which is prepared from the arabinoxylan alone or the compound of the arabinoxylan and the konjac flour, can effectively reduce the alcoholic gastrointestinal mucosa and liver injury.
Description
Technical Field
The invention belongs to the technical field of medicines and foods, and particularly relates to arabinoxylan and a compound for preventing/treating gastrointestinal mucosa and liver injury.
Background
Drinking is a traditional preference for people and gradually has social interaction properties, but excessive drinking can not only cause liver damage, but also damage to blood, digestion, cardiovascular and central nervous systems to different degrees. Alcohol is an organic solvent, readily penetrates biological membranes, can be absorbed through respiratory and digestive mucosa, and is absorbed in a small part by skin and other mucosa, and the speed and degree of absorption depend on the alcohol concentration gradient at the absorption site, the permeability of the membrane and the local blood flow.
The liver is the main organ of alcohol metabolism, more than 90% of alcohol ingested by the body is metabolized in the liver, and after entering the liver, the alcohol is oxidized into acetaldehyde through Alcohol Dehydrogenase (ADH), liver microsome alcohol oxidase system (MEOS) and catalase. Alcoholic liver injury (alcoholic liver injury) is a liver disease caused by long-term or heavy drinking, is manifested as fatty liver at the initial stage, and further can develop into alcoholic hepatitis, hepatic fibrosis and liver cirrhosis, and serious patients can induce liver cancer and liver function failure, and is listed as one of the main causes of death caused by liver diseases in western countries. The first step of the alcohol metabolism process (i.e., the step of metabolizing ethanol to acetaldehyde) is mainly achieved by two oxidation systems, one is an intracytoplasmic ethanol oxidation system through which approximately 80% of the ethanol ingested into the body is metabolized, and the other is a Microsomal Ethanol Oxidation System (MEOS) through which approximately 20% of the ethanol ingested into the body is metabolized. The enzyme participating in ethanol metabolism in MEOS is mainly cytochrome P4502E1 (cytochromeP 4502E1, CYP2E 1), and can generate obvious induction effect on the MEOS after long-term drinking. When the concentration of ethanol is lower, the ethanol is mainly decomposed by an intracytoplasmic ethanol oxidation system; if the ethanol concentration is too high, the metabolism must be carried out by means of MEOS at the same time. The high concentration of ethanol in vivo caused by long-term drinking can induce the massive expression of the key enzyme CYP2E1 in MEOS, and at the moment, the ethanol is mainly metabolized by the MEOS to generate a large amount of active oxygen free radicals, which is one of the important mechanisms of the ethanol for causing liver injury.
The gastrointestinal tract is also involved in alcohol metabolism, the gastrointestinal tract mucosa has certain metabolic capacity on alcohol, namely First Pass Metabolism (FPM), the normal gastrointestinal tract mucosa function is maintained, the action time of alcohol and gastrointestinal tract mucosa Alcohol Dehydrogenase (ADH) is prolonged, the first pass metabolic level is enhanced, and the blood alcohol concentration (SEC) is reduced. Alcohol enters the intestinal tract and is metabolized into acetaldehyde by microorganisms, the acetaldehyde can increase the permeability of gastrointestinal tract cells, the probability of leakage of enterogenic endotoxin to liver blood circulation is improved, secondary attack is formed, and liver injury is aggravated. In addition, the high permeability of small intestine to pathogenic factors such as endotoxin and bacteria can cause fever, leukocytosis, microcirculation disturbance, shock, multiple organ failure, etc. Thus, although alcohol is metabolized to a much lesser extent in the gastrointestinal tract than the liver, protection of the gastrointestinal mucosa remains of great significance in reducing alcoholic liver injury.
Arabinoxylan (AX) is an important functional hemicellulose in the cell walls of the outer layers and endosperm of cereals, and AX is mainly distributed in grain crops such as wheat, barley, oat, rice, sorghum and millet, and is also abundant in crops such as linseed and banana peel. The structure of AX is formed by connecting xylan with beta-D-xylopyranose residues through beta- (1 → 4) glycosidic bonds as a main chain and alpha-L-arabinofuranose as a side chain. The beta-D-xylose residue may be mono-substituted at the C2 and C3 positions with alpha-L-arabinofuranose, or may be di-substituted at the C2 and C3 positions with alpha-L-arabinofuranose. In addition, ferulic acid is present in ester linkage at position C5 of certain arabinosyl groups, which are normally linked at position C3 of the xylose residue, the presence of ferulic acid being important for the functional properties of AX. An important indicator of the structural characteristics of arabinoxylan is the average degree of substitution of its xylan backbone, i.e. the relative number of Ara/Xly (a/X) and arabinose residues and the differences in substitution; this is the main reason for differences in properties such as solubility, solution viscosity, gelling properties, and degree of enzymatic action of arabinoxylans. According to the difference of the solubility of arabinoxylan in water, the arabinoxylan is classified into water-soluble arabinoxylan (WSAX) and water-insoluble arabinoxylan (WISAX), and compared with WSAX, WISAX has larger molecular weight, high branching degree (lower ratio of arabinose to xylose) and higher ferulic acid content. AX improves dough properties and bread baking quality well and can be developed as a thickener, humectant, bread additive, etc. AX has a number of important physiological functions; as dietary fiber, has the effects of relaxing bowel, losing weight and beautifying; as an immunomodulator, the composition can activate Natural Killer (NK) cells, T and B lymphocytes and enhance the immune function of human body; in addition, AX also has various physiological functions of regulating blood sugar level, reducing blood lipid, resisting oxidation, promoting intestinal peristalsis, resisting tumor, improving immunity, etc. However, there is no report on the use of arabinoxylan for the prevention/treatment of alcoholic gastrointestinal mucosal and liver damage.
Water extraction, chemical extraction, enzyme extraction and mechanical auxiliary extraction are widely applied to AX production, and an alkali solvent method and a mechanical auxiliary method have higher extraction efficiency according to the extraction yield of AX, but are difficult to apply to industrial production due to the problems of cost, instrument safety, environment and the like; the yield of the enzyme method is lower, but the enzyme method can be combined with a mechanical auxiliary method and a chemical solvent method to improve the yield of AX; the mechanical auxiliary method has the function of improving the yield of AX, wherein the steam explosion method and the extrusion method both have the advantage of environmental friendliness and are suitable for industrial production. The existing research on AX also focuses on the aspects of process conditions such as yield and purity, intestinal microecology, physiological metabolism, body immune function intervention and the like, and the research on the protection function of AX or a compound thereof for preventing/treating alcoholic gastrointestinal mucosa and liver injury is not reported.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, provides arabinoxylan and a compound for preventing/treating gastrointestinal mucosa and liver injury, and specifically provides a food, a health product or a medicine prepared from the arabinoxylan alone or the compound of the arabinoxylan and konjac flour and used for preventing/treating alcoholic gastrointestinal mucosa and liver injury, which can effectively reduce the injury of alcohol to the gastrointestinal mucosa and the liver.
Application of arabinoxylan or its compound with rhizoma Amorphophalli powder in preparing food, health product or medicine for preventing/treating alcoholic gastrointestinal mucosa and liver injury is provided.
In the compound of the arabinoxylan and the konjac flour, the mass percentage of the arabinoxylan is 1-99%.
Preferably, the arabinoxylan has a glycan content of > 60%.
Preferably, the glucomannan content in the konjac flour is > 60%.
The arabinoxylan is prepared by pretreating plant raw materials, removing impurities, leaching with alkali liquor, and dealkalizing and purifying.
The plant material comprises one or more of straw, corncob, bran or wood with hemicellulose component mainly comprising arabinoxylan.
Preferably, the specific preparation method of the arabinoxylan is as follows:
(1) crushing the plant raw materials, adding 8-12 times of NaOH solution with weight and mass concentration of 0.2-1.0%, carrying out heat preservation treatment at 40-90 ℃ for 1.5-2.5 h, replacing and washing with clear water, and then filtering and dehydrating to obtain the clean fiber.
(2) Adding 6-10 times of NaOH solution with the weight and the mass concentration of 8-12% into the obtained clean fiber, performing heat preservation extraction at 55-65 ℃ for 5.5-6.5 h, settling, centrifuging, collecting primary supernatant, performing continuous countercurrent displacement on residues by using NaOH solution with the weight of 8-12 times and the mass concentration of 3-5%, settling, centrifuging, collecting secondary supernatant, and combining the primary supernatant and the secondary supernatant to obtain the arabinoxylan aqueous solution.
(3) And (3) dealkalizing the arabinoxylan alkali solution, adding acid to neutralize the solution until the pH value is 6.0-8.0, concentrating and dehydrating the solution, adding clear water to perform continuous replacement desalting treatment, and drying the solution to obtain the arabinoxylan alkali solution.
Preferably, the first sedimentation centrifugation in the step (2) is performed by using a horizontal spiral sedimentation centrifuge, and the second sedimentation centrifugation is performed by using a butterfly centrifuge with a higher separation factor.
The dealkalization mode in the step (3) can adopt any one or combination of an ultrafiltration membrane filtration mode and an electrodialysis membrane replacement mode. Preferably, the ultrafiltration membrane has a cut-off of less than or equal to 10 kDa.
The acid used in the step (3) is any one of hydrochloric acid, citric acid or acetic acid, and the mass concentration is 4-6%.
Concentrating and dehydrating in the step (3) by adopting an ultrafiltration membrane filtration mode; preferably, the ultrafiltration membrane has a cut-off of less than or equal to 10 kDa.
The arabinoxylan alone or the compound of the arabinoxylan and the konjac flour is selectively added with conventional auxiliary materials and prepared into any acceptable dosage forms such as granules, powder, tablets, capsules or suspensions according to the conventional process, and the products can be used as common food, health food or medicines.
The arabinoxylan can be added into any other processed food or food material, and can exert function via different food carriers. The different food carriers comprise rice, flour products, dairy products, vegetable protein products, dry and fresh fruit juice products and the like.
The invention has the beneficial effects that:
the content of polysaccharide in the arabinoxylan is more than 60 percent, and the content of glucomannan in the konjac flour is more than 60 percent, wherein the arabinoxylan can be commercial arabinoxylan, and can also be the arabinoxylan prepared by the preparation method. The arabinoxylan is a polysaccharide main chain formed by connecting beta-D-xylopyranose residues through beta- (1 → 4) glycosidic bonds, and is also connected with arabinose, glucuronic acid and other groups as branched chains; when the polysaccharide content in the arabinoxylan is more than 60%, the arabinoxylan contains a large amount of water-insoluble arabinoxylan, maintains the natural macromolecular characteristics of the arabinoxylan, can be uniformly dispersed in water, has increased viscosity with the reduction of pH value, is even in a gel state, is difficult to decompose and destroy by gastric acid, and has little influence on the environment in the stomach and the small intestine. The stable physicochemical property and excellent physiological function make the arabinoxylan have the potential of being developed into a novel gastrointestinal mucosa protective agent. Konjaku flour is extracted from konjaku, and mainly contains glycan, called glucomannan, with mannose and glucose as two sugar groups, which are polymerized into a main chain by beta- (1 → 4) glycosidic bond. Glucomannan has various characteristics of hydrophile, thickening, stability, emulsification, suspension, gel, film formation and the like, is similar to the arabinoxylan, has good stability in stomach and small intestine due to lack of corresponding degrading enzyme, can be spread on the surface of gastrointestinal mucosa to form a mucosa-like protective layer, and reduces the direct contact and stimulation of alcohol to the mucosa; therefore, the content of glucomannan in the konjac flour is limited to be more than 60%, so that the protective effect of the compound on gastrointestinal mucosa is favorably improved.
In the preparation method of the arabinoxylan, the raw materials are deeply purified and extracted by dilute alkali low-temperature pretreatment, the food safety is high, the obtained arabinoxylan is also a high-purity macromolecular polysaccharide, and simultaneously contains water-soluble arabinoxylan and water-insoluble arabinoxylan, the content of hydrolysable polysaccharide in the arabinoxylan is up to more than 80%, the natural macromolecular characteristics of the arabinoxylan are greatly maintained, and the protective effect on gastrointestinal mucosa is strong.
The arabinoxylan also has the function of slowing down the gastric emptying speed, is favorable for improving the first-pass metabolism level and reducing the blood alcohol concentration, and simultaneously has a plurality of physiological functions of dietary fibers. The invention prepares the arabinoxylan into food, health care products or medicines which can be used for preventing/treating alcoholic gastrointestinal mucosa and liver injury, and is suitable for preventing or treating gastrointestinal mucosa and liver injury caused by long-term drinking or taking. The compound of the araboxylan and the konjac flour has more obvious curative effect than that of the araboxylan used alone, and can reach the level close to or even better than the effect of the positive medicament of the Oimera.
After the arabinoxylan and the glucomannan are fermented by specific microorganisms in the intestinal tract, short-chain fatty acids such as acetic acid, propionic acid, butyric acid and the like are highly produced, and the short-chain fatty acids participate in the regulation and control of metabolism, nerves and immune systems through the small-molecule organic acids. Wherein, the butyric acid is an energy substance of the epithelial cells of the digestive tract, can repair the epithelial cells of the damaged digestive tract and effectively inhibit the generation and the development of inflammatory reaction of the digestive tract. The acetic acid level is negatively correlated with inflammatory indexes of liver, kidney and other organs, and propionic acid participates in regulation and control of liver lipid metabolism, and has the functions of improving the activity of antioxidant enzyme of epithelial tissues of gastrointestinal wall, preventing fatty liver and improving gastrointestinal mucosa system. The arabinoxylan can also proliferate various known intestinal probiotics, and the probiotics can effectively reduce the generation of enterogenic endotoxins such as ammonia, phenols, indoles and the like, and play roles in relaxing bowel and improving immunity. In addition, the arabinoxylan is a nutritional ingredient which is taken in a large amount along with the main grain of the grains for a long time since the culture civilization of human farming, has extremely low possibility of interfering most medicaments, and can be mixed with other gastric mucosa and liver protection medicaments to improve the effect.
Drawings
FIG. 1 is a representative diagram of the damage of the gastric mucosa of rats in a normal control group, a model control group and a positive control group;
FIG. 2 is a representative diagram of the gastric mucosal injury in rats in the group of low dosage arabinoxylan, high dosage arabinoxylan, arabinoxylan complex (I) and arabinoxylan complex (II);
FIG. 3 is a pathological section of rats with gastric mucosa injury in model control group, arabinoxylan high dose group, arabinoxylan complex (I) group and arabinoxylan complex (II) group.
Detailed Description
In order to describe the present invention in more detail, the present invention will be further described with reference to the following examples.
Example 1
The specific preparation method of the arabinoxylan comprises the following steps:
(1) selecting 10kg of dry mildew-free cereal bran, adding 100kg of NaOH solution with the mass concentration of 0.2%, carrying out heat preservation treatment at 40 ℃ for 2h, replacing and washing with clear water, and then filtering and dehydrating to obtain clean fibers.
(2) Adding 80kg of NaOH solution with the mass concentration of 8% into the obtained clean fiber, performing heat preservation extraction at 60 ℃ for 6h, performing sedimentation centrifugation on the extract by using a horizontal spiral sedimentation centrifuge to collect primary supernatant, performing continuous countercurrent replacement on the residue by using 100kg of NaOH solution with the mass concentration of 4%, performing sedimentation centrifugation on the replacement solution by using a butterfly centrifuge to collect secondary supernatant, and combining the primary supernatant and the secondary supernatant to obtain 165kg of the arabinoxylan aqueous alkali.
(3) Dealkalizing the arabinoxylan alkali solution by an ultrafiltration membrane, slowly adding hydrochloric acid with the mass concentration of 5% while stirring to neutralize the solution to a pH value of 6.0-8.0, concentrating and dehydrating by the ultrafiltration membrane, adding clear water to perform continuous replacement desalting treatment, and performing spray drying to obtain 1.1kg of the arabinoxylan. The cutoff molecular weight of the ultrafiltration membrane is less than or equal to 10 kDa. The content of hydrolyzable polysaccharide in the finally obtained arabinoxylan is 82%, and all pollutant indexes are within the national standard range of grain food.
Example 2
The specific preparation method of the arabinoxylan comprises the following steps:
(1) selecting 1kg of dried, mildew-free and crushed corncobs, adding 10kg of NaOH solution with the mass concentration of 1.0%, carrying out heat preservation treatment at 90 ℃ for 2h, replacing and washing with clear water, and then filtering and dehydrating to obtain clean fibers.
(2) Adding 8kg of NaOH solution with the mass concentration of 10% into the obtained clean fiber, performing heat preservation extraction at 60 ℃ for 6h, performing sedimentation centrifugation on the extract by using a horizontal spiral sedimentation centrifuge to collect primary supernatant, performing continuous countercurrent replacement on the residue by using 10kg of NaOH solution with the mass concentration of 4%, performing sedimentation centrifugation on the replacement solution by using a butterfly centrifuge to collect secondary supernatant, and combining the primary supernatant and the secondary supernatant to obtain 16kg of the arabinoxylan aqueous alkali.
(3) And (2) after the arabinoxylan alkali solution is subjected to displacement dealkalization treatment by an electrodialysis membrane, slowly adding citric acid with the mass concentration of 5% while stirring to neutralize the solution to the pH value of 6.0-8.0, performing concentration and dehydration by using an ultrafiltration membrane with the molecular weight cutoff of less than or equal to 10kDa, adding clear water to perform continuous displacement desalination treatment, and performing spray drying to obtain 0.17kg of arabinoxylan. The content of hydrolyzable polysaccharide in the finally obtained arabinoxylan is 88%, and all pollutant indexes are within the national standard range of grain food.
Example 3
Arabinoxylan complex (i) for preventing/treating gastrointestinal mucosal and liver injury was prepared by mixing 80 parts by weight of arabinoxylan of example 1 and 20 parts by weight of commercially available konjac flour having a glucomannan content of 85%.
Arabinoxylan complex for preventing/treating gastrointestinal mucosa and liver injury, which was obtained by mixing 95 parts by weight of arabinoxylan of example 1 and 5 parts by weight of commercially available konjac flour having a glucomannan content of 85%.
Arabinoxylan complex for preventing/treating gastrointestinal mucosa and liver injury, which is obtained by mixing 80 parts by weight of arabinoxylan of example 2 and 20 parts by weight of commercially available konjac flour having a glucomannan content of 85%.
Arabinoxylan complex (iv) for preventing/treating gastrointestinal mucosal and liver injury was obtained by mixing 95 parts by weight of arabinoxylan of example 2 and 5 parts by weight of commercially available konjac flour having a glucomannan content of 85%.
In order to verify the efficacy of the arabinoxylan or the complex of arabinoxylan and konjac flour of the present invention in preventing alcoholic gastrointestinal mucosa and liver damage, the applicant conducted the following tests:
test subjects: SD rats, female, 6 weeks old, weight 200 g-220 g/animal, 56 total, purchased from Silikedada test animals, Inc., Hunan province, license number: SCXK (Xiang) 2019-.
The rats were fed adaptively for 3 days and were randomly divided into 7 groups, a normal control group, a model control group, a positive control group, an arabinoxylan low dose group, an arabinoxylan high dose group, an arabinoxylan complex group i, and an arabinoxylan complex group ii. The positive control group is gavaged with 40mg/kg per day, the low-dose group of the arabinoxylan is gavaged with 1g/kg of the arabinoxylan prepared in the embodiment 1 per day, the high-dose group of the arabinoxylan is gavaged with 1.5g/kg of the arabinoxylan prepared in the embodiment 1 per day, the group of the arabinoxylan complex is gavaged with 1g/kg of the arabinoxylan complex per day, the normal control group and the model control group are gavaged with physiological saline, the gastric perfusion capacity is 4 mL/mouse, free drinking water is realized during the test period, and the rat standard is used for maintaining the feeding with the pellet feed.
After 14 days of continuous dosing, all rats were fasted for 24h, during which time water was freely available. The group II is continuously gavaged for 14 days according to the dosage, fasting is carried out for 24 hours, and the group II is gavaged for one time 1 hour before the treatment with alcohol. Except for the normal control group, the other groups were gavaged with absolute ethanol to induce gastric injury, 1.0 mL/mouse. After 1 hour of the gavage with absolute ethanol, blood serum was collected from the carotid artery, and the contents of ALT (alanine transferase) and AST (aspartate transferase) in the blood serum were measured, and the results are shown in table 1. All rats are dislocated and killed after blood collection, the stomach is taken, the rat is cut along the greater curvature of the stomach, PBS is used for cleaning, the gastric mucosa is developed, water is sucked by paper, the damage condition of the gastric mucosa layer is carefully observed, and the representative graph of the damage condition of the gastric mucosa of the rats in each test group is shown in figures 1-2. Meanwhile, the number of bleeding points is recorded, the length and the width of an ulcer strip are measured by using a vernier caliper, histological scoring is carried out on gastric lesions according to a macroscopic evaluation scoring table for calculating the ulcer degree according to the national food and drug administration (CFDA publication No. 107, revised in 2012), the injury incidence and the injury inhibition rate of rats in each group are calculated, the stomach mucosa injury scoring standard is shown in a table 2, and the injury incidence and the injury inhibition rate are shown in a table 3.
After histological scoring, each stomach was cut in half, and half of the stomach was used for tissue homogenization to detect the contents of SOD and MDA in the gastric mucosa tissue (if the homogenization was not immediately detected, the contents could be stored at-80 ℃), and the gastric mucosa damage index was calculated, and the damage index results are shown in table 4. The other half of the stomach, where the gastric mucosa is most severely damaged, is excised and then processed as follows to prepare HE stained sections and observe the pathological changes of the sections (if processing is not done immediately, the excised damaged parts can be fixed in neutral formalin solution):
(1) dehydrating each tissue according to the following steps: 50% ethanol (90min) → 70% ethanol (90min) → 85% ethanol (90min) → 95% ethanol (90min) → 100% ethanol (60 min).
(2) The dehydrated tissue is transparent according to the following steps: 1/2 ethanol +1/2 xylene → xylene for 60min each step.
(3) The transparent tissue was waxed at 62 ℃ according to the following procedure: 1/2 xylene +1/2 paraffin (90min) → paraffin I (120min) → paraffin II (120 min).
(4) Placing the tissue in a mold containing paraffin, placing the mold, pouring molten paraffin, and cooling.
(5) Fixing the embedded tissue wax block in a slicer, cutting into slices with thickness of about 4 μm, spreading in a water bath, taking out the slices on a polylysine film-attached glass slide, numbering, and baking at 65 deg.C for 4 h.
(6) And E, performing HE staining on the section by using an HE staining kit, and observing the pathological change condition of the section. The pathological section of the damage condition of the gastric mucosa of rats in each group is shown in figure 3.
The calculation formulas of the injury incidence, the injury inhibition rate and the injury index are as follows:
(1) incidence of injury (%) = number of bleeding or ulceration rats appearing in a group/number of rats in the group × 100%
(2) Damage inhibition (%) = (A-B)/A × 100% (A is damage integral of model control group, B is damage integral of other test group)
(3) Injury index = sum of group injury scores/number of animals in group
Alanine transferase and aspartate transferase in serum are labeled enzymes reflecting the degree of liver injury, and as is apparent from table 1, the enzyme index of rats in a model control group is seriously increased to indicate that the liver is seriously injured, while the enzyme index of rats given with the arabinoxylan or the compound thereof of the invention tends to be normal, which indicates that the arabinoxylan and the compound thereof of the invention have a protective effect on alcoholic liver injury. As can be seen from Table 3, ethanol can obviously cause bleeding of rat gastric mucosa, which causes gastric mucosa injury, and the arabinoxylan and the compound thereof can effectively inhibit the rat gastric mucosa injury caused by alcohol. As can be seen from Table 4, both the arabinoxylans and the complexes thereof of the invention can significantly reduce the damage index of ethanol to rat gastric mucosa. As can be seen from figures 1-2, the arabinoxylan and the compound thereof can obviously reduce the damage degree of ethanol to rat gastric mucosa. As can be seen from FIG. 3, the degeneration and necrosis of the epithelial cells of the gastric mucosa of the rats in the model control group cause the infiltration of inflammatory cells which account for about 45-60% of the epithelium; the gastric mucosa of the rats in the high-dose arabinoxylan group also has inflammatory cell infiltration, but the damage degree is smaller than that of a model control group, and the damaged part of the rats accounts for about 14-29% of the epithelium; the structure of the gastric mucosa of rats in the first and second groups of the arabinoxylan compound is basically normal, and inflammatory cell infiltration is rarely seen. The pathological results obtained by whole gastric mucosa observation and HE staining slice observation are basically consistent with the general (integral) observation score result, and the arabinoxylan and the compound thereof can be judged to have protective and therapeutic effects on alcoholic gastric mucosa injury.
According to the stipulation in the evaluation method (manuscript in request) for auxiliary protection function of gastric mucosa injury provided by food and drug administration: the invention relates to a method for preparing a compound of arabinoxylan and a compound of arabinoxylan, which is characterized in that the arabinoxylan and the compound of arabinoxylan can reduce the incidence rate of the gastric mucosa injury of rats, can obviously reduce the gastric mucosa injury index of rats and improve the gastric mucosa injury inhibition rate of rats, and can also be supported pathologically, so that the arabinoxylan and the compound of arabinoxylan can be judged to have the effects of prevention, auxiliary protection and treatment on the gastric mucosa.
The present invention is not limited to the above preferred embodiments, and various other forms of the product can be obtained by anyone with the benefit of the present disclosure, but any variation on the details thereof, which is the same as or similar to the technical solution of the present invention, falls within the protection scope of the present invention.
Claims (10)
1. Application of arabinoxylan or its compound with rhizoma Amorphophalli powder in preparing food, health product or medicine for preventing/treating alcoholic gastrointestinal mucosa and liver injury is provided.
2. The use according to claim 1, wherein the mass ratio of the arabinoxylan to the konjac flour in the arabinoxylan-konjac flour composite is 1-99%.
3. Use according to claim 1 or 2, wherein the arabinoxylan has a glycan content > 60%.
4. Use according to claim 1 or 2, wherein the glucomannan content of the konjac flour is > 60%.
5. The use of claim 1 or 2, wherein the arabinoxylan is prepared from plant raw materials by pretreatment, impurity removal, alkali extraction, and dealkalization purification.
6. The use according to claim 5, wherein the plant material comprises one or more of straw, corncob, bran or wood having a hemicellulose component comprising arabinoxylan as a main component.
7. The use according to claim 5, wherein the arabinoxylan is prepared by the following specific method:
(1) crushing plant raw materials, adding 8-12 times of NaOH solution with the weight and the mass concentration of 0.2-1.0%, carrying out heat preservation treatment at 40-90 ℃ for 1.5-2.5 h, replacing and washing with clear water, and then filtering and dehydrating to obtain clean fibers;
(2) adding 6-10 times of NaOH solution with the weight and the mass concentration of 8-12% into the obtained clean fiber, performing heat preservation extraction at 55-65 ℃ for 5.5-6.5 h, settling, centrifuging and collecting primary supernatant, performing continuous countercurrent displacement on the residue by using 8-12 times of NaOH solution with the mass concentration of 3-5%, settling and centrifuging the displacement solution, collecting secondary supernatant, and combining the primary supernatant and the secondary supernatant to obtain an arabinoxylan alkali solution;
(3) and (3) dealkalizing the arabinoxylan alkali solution, adding acid to neutralize the solution until the pH value is 6.0-8.0, concentrating and dehydrating the solution, adding clear water to perform continuous replacement desalting treatment, and drying the solution to obtain the arabinoxylan alkali solution.
8. The use of claim 7, wherein the dealkalization in step (3) can be performed by one or a combination of ultrafiltration membrane filtration and electrodialysis membrane replacement.
9. The use according to claim 1, wherein the arabinoxylan alone or the complex of the arabinoxylan and the konjac flour is optionally added with conventional adjuvants, and made into granules, powders, tablets, capsules or suspensions according to conventional processes, and used as common food, health food or medicine.
10. The use according to claim 1 or 9, wherein the arabinoxylan alone or the complex of the arabinoxylan with the konjac flour is added to any other processed food or food material, functioning through different food carriers; the different food carriers comprise rice, flour products, dairy products, vegetable protein products and dry and fresh fruit juice products.
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