JP6753618B2 - Composition for suppressing increase in triglyceride in blood - Google Patents

Description

The present invention relates to a composition for suppressing triglyceride elevation in blood, and more particularly to a composition for suppressing triglyceride elevation in blood containing indigestible dextrin and a specific other component as active ingredients.

Indigestible dextrin is known to have various functions for adjusting the condition of the body, and one of them is known to have an inhibitory effect on the increase of triglyceride in blood. However, the action and effect of the single action is not always sufficient.

On the other hand, regarding the function of indigestible dextrin, for example, an IgA secretagogue containing indigestible dextrin as an active ingredient (see Patent Document 1) and for patients with renal failure containing indigestible dextrin as an active ingredient. A blood cresol lowering agent (see Patent Document 2) has been proposed.

Japanese Unexamined Patent Publication No. 2014-152125 Japanese Unexamined Patent Publication No. 2014-148467

An object of the present invention is to provide a composition for suppressing the increase in triglyceride in blood, which has a dramatically improved effect of suppressing the increase in triglyceride in blood.

The present inventors diligently investigated and studied the inhibitory effect of indigestible dextrin on the increase in triglyceride in blood, and found that by combining the indigestible dextrin with a specific component, the inhibitory effect on the increase in triglyceride in blood was leapfrogged. We have found that it can be improved and completed the present invention. That is, by combining a component having little or little effect of suppressing the increase in triglyceride in blood with indigestible dextrin, the increase in triglyceride in blood is compared with the case of indigestible dextrin alone. We have found that the inhibitory effect can be dramatically improved.

That is, the present invention comprises indigestible dextrin, pine bark extract, barley young leaf treatment, collagen, pectin, tannin, alanine, valine, vitamin A, vitamin B1, niacin, sodium pantothenate, vitamin E, and selenium compound. The present invention relates to a composition for suppressing the increase in neutral fat in blood, which comprises at least one component selected from the group consisting of the above as an active ingredient.

The composition for suppressing the increase in triglyceride in blood of the present invention may be obtained by adding an active ingredient.

In the composition for suppressing the increase in triglyceride in blood of the present invention, the compounding mass ratio of indigestible dextrin and other active ingredients is preferably in the range of 0.5: 1 to 70: 1.
In addition, the composition for suppressing the increase in triglyceride in blood of the present invention is preferably a tablet, a capsule, a powder, a granule, or a liquid.

The present invention also relates to a method for suppressing the increase in triglyceride in blood (excluding medical practice), which comprises ingesting the composition for suppressing the increase in triglyceride in blood of the present invention.

According to the composition for suppressing the increase in triglyceride in blood of the present invention, the effect of suppressing the increase in triglyceride in blood can be dramatically improved as compared with the case of indigestible dextrin alone.

It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer film when the composition for suppressing the increase in triglyceride in blood of this invention is applied, and is the pine bark extract together with indigestible dextrin. The result when using is shown. It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer film when the composition for suppressing the increase in triglyceride in blood of this invention is applied, and is the figure which shows the barley young leaf, collagen together with indigestible dextrin. , Pectin, and tannic acid are shown. It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer film when the composition for suppressing the increase in triglyceride in blood of this invention was applied, and alanine was used together with indigestible dextrin. The result of the case is shown. It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer membrane when the composition for suppressing the increase in triglyceride in blood of this invention was applied, and valine was used together with indigestible dextrin. The result of the case is shown. It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer film when the composition for suppressing the increase in triglyceride in blood of this invention is applied, and vitamin A is used together with indigestible dextrin. The result when there is is shown. It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer film when the composition for suppressing the increase in triglyceride in blood of this invention is applied, and is the figure which shows vitamin B1, niacin together with indigestible dextrin. , The results when sodium pantothenate is used are shown. It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer film when the composition for suppressing the increase in triglyceride in blood of this invention is applied, and is using vitamin E together with indigestible dextrin. The result when there is is shown. It is a figure which shows the concentration of the triglyceride (TG) secreted after being absorbed into the Caco-2 monolayer film when the composition for suppressing the increase in triglyceride in blood of this invention is applied, and is the figure which shows the triglyceride (TG) concentration | The results when using sodium selenite) are shown.

The composition for suppressing the increase in triglyceride in blood of the present invention includes indigestible dextrin, pine bark extract, barley young leaf treatment, collagen, pectin, tannin, alanine, valine, vitamin A, vitamin B1, niacin, and pantothenic acid. It is characterized by containing at least one component (hereinafter, may be referred to as another component) selected from the group consisting of sodium, vitamin E, and selenium compounds as an active ingredient.

[Indigestible dextrin]
The indigestible dextrin, which is the active ingredient of the composition for suppressing the increase in blood neutral fat of the present invention, is an indigestible polysaccharide that is difficult to be hydrolyzed by human digestive enzyme (amylase). For example, by heating starch. After being hydrolyzed, it can be hydrolyzed with amylase to purify components that are not easily hydrolyzed. This indigestible dextrin is commercially available in the form of powder, fine granules, granules, etc., and these commercially available products can be used. Further, since the indigestible dextrin is water-soluble, an aqueous solution may be used.

[Other ingredients]
(Pine bark extract)
Examples of the pine used as a raw material for the pine bark extract include French coastal pine (Pinus Martinma), caramatsu, black pine, Japanese red pine, etc. Among these, French coastal pine (Pinus) rich in proanthocyanidins. Martima) is preferred. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water, hot water), ethanol, or hydrous ethanol can be used. The extract contains a high concentration of oligomeric proanthocyanidins in addition to proanthocyanidins. For example, it is preferable that the extract contains 20% by mass or more of oligomeric proanthocyanidins with respect to the dry mass of the extract.

(Processed barley young leaves)
As the barley used as a raw material for the processed barley young leaf products, two-row barley, six-row barley and the like can be used. As the barley young leaf processed product, a processed product such as a crushed barley young leaf product, squeezed juice, or an extract can be used. Examples of the pulverized product include powders and granules. The juice or extract may be liquid, but can also be used as a paste or dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water, hot water), ethanol, or hydrous ethanol can be used.

(collagen)
Collagen may be animal-derived collagen or synthetic collagen. In addition to collagen protein, collagen peptide obtained by hydrolyzing collagen protein or collagen molecule is treated with protease to provide a telopeptide moiety. Includes atelocollagen from which. As the collagen derived from animals, collagen derived from fish is particularly preferable. The average molecular weight (weight average molecular weight) of collagen is not particularly limited, but is preferably 500 to 100,000, more preferably 1,000 to 50,000, for example.

(Pectin)
The pectin may be a plant-derived pectin or a synthetic pectin. Examples of plant-derived pectin include extracts from fruits and vegetables such as apples and citrus fruits. The pectin is classified into HM pectin having a high degree of esterification and LM pectin having a low degree of esterification, and any pectin may be used. Further, as the pectin, commercially available pectin as a gelling agent, a thickener, or the like can be used.

(Tannin)
The tannins may be hydrolyzable tannins or condensed tannins, for example, obtained from persimmon fruit, chestnut astringent skin, quintuplet, tamarind seed coat, cod powder, gall nut, and mimosa bark. Extracts can be mentioned. In addition, tannic acid (C ₇₆ H ₅₂ O ₄₆ ) can be used.

The composition for suppressing the increase in triglyceride in blood of the present invention is preferably an agent for suppressing the increase in triglyceride in blood used for suppressing the increase in triglyceride in blood. Such an agent for suppressing the increase in triglyceride in blood contains indigestible dextrin and a predetermined other component, and can be distinguished from other products as a product in that it is used for suppressing the increase in triglyceride in blood. If this is the case, the present invention is not particularly limited, and for example, a product indicating that the body, packaging, instruction manual, or advertisement of the product according to the present invention has a function of suppressing the increase in triglyceride in blood is indicated. It is included in the scope of the present invention. For example, so-called health foods such as pharmaceuticals (including non-pharmaceutical products), foods for specified health use, foods with nutritional function, foods with functional claims, and other so-called health foods whose efficacy has been approved by a prescribed organization, feeds, etc. Can be mentioned. In so-called health foods, "suppress the rise of triglyceride", "for those who are concerned about triglyceride", "suppress fat absorption", "for those with high triglyceride", etc. are displayed. Things can be illustrated. These health foods may be taken separately from meals, but are preferably taken at the time of meals, for example, between 1 hour before meals and 1 hour after meals, 0. It is more preferably taken between 5 hours and 0.5 hours after a meal, and even more preferably from 0.5 hours before a meal to the beginning of a meal or during a meal.

The form of the composition for suppressing the increase in triglyceride in blood of the present invention includes, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, and the like. Examples thereof include round agents, paste agents, cream agents, capsule agents, gel agents, chewable agents, stick agents and the like. Among these, the forms of tablets, capsules, powders, granules, and liquids are particularly preferable. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant powdered beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc., and instant granule beverages. Can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The content of the indigestible dextrin and other components (active ingredients) in the composition for suppressing the increase in triglyceride in blood of the present invention may be appropriately contained within the range in which the effect is exhibited.

Generally, when the composition for suppressing the increase in triglyceride in blood of the present invention is a drug or supplement (tablet, capsule), the active ingredient is contained in an amount of 0.01 to 100% by mass in terms of dry mass. It is more preferable that it is contained in an amount of 0.1 to 85% by mass, and it is more preferable that it is contained in an amount of 0.5 to 70% by mass.

When the composition for suppressing the increase in triglyceride in blood of the present invention is a packaged beverage (liquid preparation), the active ingredient is preferably contained in an amount of 0.1 to 10% by mass in terms of dry mass, and is 0. It is more preferably contained in an amount of 3 to 6% by mass, and further preferably contained in an amount of 0.5 to 4% by mass.

When the composition for suppressing the increase in triglyceride in blood of the present invention is an instant powdered beverage (powder) or an instant granule beverage (granule), the active ingredient is 10 to 100% by mass in terms of dry mass. It is preferably contained, more preferably 50 to 100% by mass, and even more preferably 80 to 100% by mass.

In order to exert the effect of the present invention particularly effectively, the active ingredient must be contained in an amount of 80% by mass or more of the entire blood triglyceride increase suppressing composition (excluding water) of the present invention in terms of dry mass. It is more preferable that the content is 90% by mass or more, more preferably 95% by mass or more, and particularly preferably 100% by mass.

The intake amount of the composition for suppressing the increase in triglyceride in blood of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the intake amount of the active ingredient per day is 500 mg / day or more. It is preferable to take it so as to be 1 g / day or more, more preferably it is taken so as to be 2 g / day or more, and it is more preferably taken so as to be 4 g / day or more. It is particularly preferable to do so.

The composition for suppressing the increase in triglyceride in blood of the present invention is divided into one container or, for example, a plurality of containers of 2 to 3 so that the daily intake becomes the above-mentioned intake, and is used as one daily dose. Can be accommodated.

In addition, the composition for suppressing the increase in blood neutral fat of the present invention may be taken separately from meals, but since it is possible to particularly effectively suppress the transient increase in blood neutral fat after meals, It is preferably taken at the time of meal, for example, it is preferable to take it between 1 hour before meal and 1 hour after meal, and it is preferable to take it between 0.5 hour before meal and 0.5 hour after meal. It is more preferable to take it from 0.5 hours before meal to the start of meal or during meal.

The compounding mass ratio of the indigestible dextrin and other components is preferably in the range of 0.5: 1 to 70: 1, preferably in the range of 0.75: 1 to 60: 1 in terms of dry mass. Is more preferable, and the range of 1: 1 to 60: 1 is more preferable, and the range of 1: 1 to 50: 1 is particularly preferable. When the blending ratio of the indigestible dextrin and other components is within the above range, the effect of the present invention can be more effectively exhibited.

The composition for suppressing the increase in triglyceride in blood of the present invention can be produced by a known formulation method by adding an ingredient other than the active ingredient permitted for oral use, if necessary.

Further, as the blood neutral fat increase suppressing composition of the present invention, in addition to the blood neutral fat increase suppressing composition containing an active ingredient, blood neutrality obtained by adding an active ingredient to foods. Examples of the fat increase suppressing composition (blood neutral fat increase suppressing food) include foods having an increased active ingredient content of the present invention as compared with ordinary foods (including natural foods). , Foods to which the active ingredient is added to the food which normally does not contain the active ingredient of the present invention can be mentioned. Further, as the composition for suppressing the increase in triglyceride in blood of the present invention, a high-fat food having a fat content of 10% by mass or more, preferably 20% by mass or more, from the viewpoint that the effect of the present invention is more effectively exhibited. A high-fat food that suppresses the increase in triglyceride in blood obtained by adding an active ingredient is preferable. The active ingredient may be added separately, at the same time, or together with other ingredients other than the active ingredient.

Examples of the food for suppressing the increase in blood neutral fat of the present invention include beverages such as carbonated beverages, nutritional beverages, fruit beverages, lactic acid beverages, smoothies, and green juice; cold confectionery such as ice cream, ice sherbet, and shaved ice; soba and udon , Harusame, Chinese noodles, instant noodles and other noodles; candy, candy, gum, chocolate, lock confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, bread and other confectionery; fishery products such as kamaboko, ham and sausage・ Processed livestock foods; dairy products such as processed milk, fermented milk, yogurt; salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and other fats and oils and their processed foods; sauces, soy sauce and other seasonings; curry, Retort pouch foods such as stew, parent and child bowl, porridge, miscellaneous dishes, Chinese bowl, katsudon, tempura bowl, beef bowl, hayashi rice, omelet rice, oden, marbo dough, dumplings, shumai, hamburger, meat balls, various sauces, various soups, etc. Can be mentioned.

The method for suppressing the increase in triglyceride in blood of the present invention is characterized by ingesting the composition for suppressing the increase in triglyceride in blood of the present invention described above, but does not include medical practice. The composition for suppressing the increase in blood neutral fat of the present invention may be ingested separately from meals, but since it can particularly effectively suppress the transient increase in blood neutral fat after meals, it can be taken in meals. It is preferable to take it at the time, for example, it is preferable to take it between 1 hour before meal and 1 hour after meal, and it is more preferable to take it between 0.5 hour before meal and 0.5 hour after meal. , It is more preferable to take it from 0.5 hours before meal to the start of meal or during meal. As the method of the present invention, for example, in a restaurant such as a restaurant, a method of suppressing the increase of triglyceride in blood by providing the composition for suppressing the increase of triglyceride in blood of the present invention together with a high-fat food is used. Can be mentioned.

Hereinafter, the present invention will be described based on examples.
[Example 1]
The suppression of triglyceride absorption (suppression of triglyceride elevation in blood) was evaluated by the Caco-2 monolayer membrane absorption and secretion test of human colon cancer-derived cells. Caco-2 cells are known to differentiate into intestinal epithelial-like cells by culturing, and intestinal resorption can be evaluated by measuring the amount of a substance permeated through the Caco-2 monolayer membrane.

1. Sample Preparation 0.2 mM lysophosphatidylcholine, 0.1 mM oleic acid, 0.1 mM 2-monooleoylglycerol, and 2 mM sodium turbodeoxycholine were adjusted and sonicated to prepare 300-500 nm micelles.

The prepared micelles and test substances (indigestible dextrin alone or indigestible dextrin + other components shown in Table 1) were added to DMEM to adjust the final concentration to the desired level.

2. Caco-2 monolayer membrane absorption and secretion test 100 μL of a sample was added to the insert well of the Caco-2 monolayer membrane and held at 37 ° C. in a 5% CO ₂ incubator. After 24 hours, 600 μL of the basement membrane-side solution was recovered, 200 μL of CHCl ₃ : MeOH = 2: 1 was added, and centrifugation was performed after vortexing. The lower layer (organic layer) was recovered, 200 μL of CHCl ₃ : MeOH = 2: 1 was added again, and centrifugation was performed after vortexing. The lower layer (organic layer) was recovered, and the organic layer was dried. 20 μL 2-propanol was added and triglyceride (TG) was measured. For TG measurement, Wako's kit (Triglyceride E-Test Wako) was used.

The results are shown in Table 1 and FIGS. 1 to 8. In each graph in the figure, for control (indigestible dextrin), "-" indicates no addition, and "+" indicates the addition of 200 μg / mL indigestible dextrin alone. In addition, for those in which other components are displayed, "-+" indicates the addition of the other component alone, and "++" indicates the addition of 200 μg / mL indigestible dextrin + the other component.

As for the indigestible dextrin, a commercially available indigestible dextrin powder was used.
For young barley leaves, dry crushed powder was used.
For the pine bark, a hot water extract (dry powder) of the bark of French coastal pine was used.
For young sweet potato leaves, dried crushed powder was used.

As shown in Table 1 and FIGS. 1 to 8, the combination of indigestible dextrin and a specific other component of the present invention synergistically reduced the amount of fat passing through the Caco-2 monolayer. Therefore, it can be seen that the composition for suppressing the increase in triglyceride in blood of the present invention can effectively suppress the increase in triglyceride in blood.

[Example 2] (Production of tablets)
Each component was mixed at the ratio shown below, and locking was performed with a locking machine.

Ingredient composition (mg)
Indigestible dextrin 135
Barley young leaf powder 150
Excipient 120
Vitamin B1 15
Citric acid anhydride 15
Alanine 10
Valine 10

It is expected that the effect of suppressing the increase in triglyceride in blood can be obtained by ingesting several tablets per day, for example.

[Example 3] (Production of capsule)
Each component was mixed at the ratio shown below to prepare granules according to a conventional method, and 250 mg of the granules were filled in capsules to prepare capsules.
Indigestible dextrin by weight 15
Pine bark extract 1
Collagen 1
Vitamin E 0.1
Excipient 5

It is expected that the effect of suppressing the increase in triglyceride in blood can be obtained by ingesting several capsules per day, for example.

[Example 4] (Production of granules)
Each component was mixed in the ratio shown below to prepare granules according to a conventional method, and 800 mg of the granules was packaged in an aluminum stick.
Indigestible dextrin by weight 15
Barley young leaves 1
Excipient 5
Citric acid anhydride 0.1
Tannic acid 0.01

It is expected that the effect of suppressing the increase in triglyceride in blood can be obtained by ingesting several bags of this granule per day, for example.

[Example 5] (Production of liquid preparation)
A container was filled with 1 part by weight of pine bark extract, 5 parts by weight of young barley leaf powder, 15 parts by weight of indigestible dextrin hydrolyzed collagen, an appropriate amount of vitamin C, and 200 ml of water, and sealed to prepare a liquid preparation.

Since the composition for suppressing the increase of triglyceride in blood of the present invention has an effect of suppressing the increase of triglyceride in blood, it is highly industrially useful.

Claims (1)

And indigestible dextrin, blood neutral fat increase suppressing food composition characterized by containing a selenium compound as an active ingredient.