JP4495349B2 - 癌の遺伝マーカーを生物学的サンプルにおいて検出するための核酸配列 - Google Patents
癌の遺伝マーカーを生物学的サンプルにおいて検出するための核酸配列 Download PDFInfo
- Publication number
- JP4495349B2 JP4495349B2 JP2000596180A JP2000596180A JP4495349B2 JP 4495349 B2 JP4495349 B2 JP 4495349B2 JP 2000596180 A JP2000596180 A JP 2000596180A JP 2000596180 A JP2000596180 A JP 2000596180A JP 4495349 B2 JP4495349 B2 JP 4495349B2
- Authority
- JP
- Japan
- Prior art keywords
- seq
- sequence
- psa
- nucleic acid
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 150000007523 nucleic acids Chemical group 0.000 title claims description 104
- 239000012472 biological sample Substances 0.000 title claims description 23
- 206010028980 Neoplasm Diseases 0.000 title description 36
- 201000011510 cancer Diseases 0.000 title description 32
- 230000002068 genetic effect Effects 0.000 title description 20
- 108010072866 Prostate-Specific Antigen Proteins 0.000 claims description 187
- 239000000523 sample Substances 0.000 claims description 158
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 142
- 230000003321 amplification Effects 0.000 claims description 141
- 108091034117 Oligonucleotide Proteins 0.000 claims description 101
- 102000039446 nucleic acids Human genes 0.000 claims description 91
- 108020004707 nucleic acids Proteins 0.000 claims description 91
- 238000001514 detection method Methods 0.000 claims description 90
- 210000002307 prostate Anatomy 0.000 claims description 90
- 238000000034 method Methods 0.000 claims description 68
- 238000003556 assay Methods 0.000 claims description 51
- 230000000295 complement effect Effects 0.000 claims description 39
- 108090000623 proteins and genes Proteins 0.000 claims description 32
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 25
- 125000003729 nucleotide group Chemical group 0.000 claims description 15
- 239000002773 nucleotide Substances 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 3
- 102000007066 Prostate-Specific Antigen Human genes 0.000 claims 16
- 102100038358 Prostate-specific antigen Human genes 0.000 description 155
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 137
- 108020004999 messenger RNA Proteins 0.000 description 68
- 101001050577 Homo sapiens Kinesin-like protein KIF2A Proteins 0.000 description 44
- 238000006243 chemical reaction Methods 0.000 description 44
- 101000892862 Homo sapiens Glutamate carboxypeptidase 2 Proteins 0.000 description 43
- 102100041003 Glutamate carboxypeptidase 2 Human genes 0.000 description 41
- 102100023426 Kinesin-like protein KIF2A Human genes 0.000 description 41
- 210000004027 cell Anatomy 0.000 description 40
- 206010060862 Prostate cancer Diseases 0.000 description 39
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 39
- 108020004414 DNA Proteins 0.000 description 37
- 102000053602 DNA Human genes 0.000 description 37
- 210000001519 tissue Anatomy 0.000 description 35
- 239000002245 particle Substances 0.000 description 27
- 108700024394 Exon Proteins 0.000 description 25
- 239000000047 product Substances 0.000 description 24
- 238000012360 testing method Methods 0.000 description 24
- 239000011886 peripheral blood Substances 0.000 description 23
- 210000005259 peripheral blood Anatomy 0.000 description 22
- RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 20
- 239000007787 solid Substances 0.000 description 19
- 238000000338 in vitro Methods 0.000 description 18
- 239000003550 marker Substances 0.000 description 18
- 210000004369 blood Anatomy 0.000 description 17
- 239000008280 blood Substances 0.000 description 17
- 108020004635 Complementary DNA Proteins 0.000 description 16
- 238000010804 cDNA synthesis Methods 0.000 description 16
- 239000002299 complementary DNA Substances 0.000 description 16
- 239000013642 negative control Substances 0.000 description 16
- 102000040430 polynucleotide Human genes 0.000 description 16
- 108091033319 polynucleotide Proteins 0.000 description 16
- 239000002157 polynucleotide Substances 0.000 description 16
- 108091093088 Amplicon Proteins 0.000 description 15
- 206010006187 Breast cancer Diseases 0.000 description 15
- 208000026310 Breast neoplasm Diseases 0.000 description 15
- 102100034343 Integrase Human genes 0.000 description 15
- 210000000265 leukocyte Anatomy 0.000 description 14
- 206010004446 Benign prostatic hyperplasia Diseases 0.000 description 13
- 208000004403 Prostatic Hyperplasia Diseases 0.000 description 13
- 210000001165 lymph node Anatomy 0.000 description 13
- 238000013518 transcription Methods 0.000 description 13
- 230000035897 transcription Effects 0.000 description 13
- 210000000481 breast Anatomy 0.000 description 12
- 230000001404 mediated effect Effects 0.000 description 12
- 206010027476 Metastases Diseases 0.000 description 11
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 11
- 230000009401 metastasis Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 9
- 239000006166 lysate Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 210000000813 small intestine Anatomy 0.000 description 9
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 8
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 8
- 101000605534 Homo sapiens Prostate-specific antigen Proteins 0.000 description 8
- 101000592517 Homo sapiens Puromycin-sensitive aminopeptidase Proteins 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 102000057032 Tissue Kallikreins Human genes 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 7
- 108060005987 Kallikrein Proteins 0.000 description 7
- 108091034057 RNA (poly(A)) Proteins 0.000 description 7
- 238000010240 RT-PCR analysis Methods 0.000 description 7
- -1 pentose sugars Chemical class 0.000 description 7
- 238000010561 standard procedure Methods 0.000 description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 108091092195 Intron Proteins 0.000 description 6
- 102000001399 Kallikrein Human genes 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 230000009089 cytolysis Effects 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- 239000013614 RNA sample Substances 0.000 description 5
- 101710137500 T7 RNA polymerase Proteins 0.000 description 5
- 210000001185 bone marrow Anatomy 0.000 description 5
- 210000003734 kidney Anatomy 0.000 description 5
- 210000004072 lung Anatomy 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 4
- 239000007995 HEPES buffer Substances 0.000 description 4
- 101710203526 Integrase Proteins 0.000 description 4
- 101710176219 Kallikrein-1 Proteins 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 4
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 4
- 238000010369 molecular cloning Methods 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 210000005084 renal tissue Anatomy 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 238000005464 sample preparation method Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 208000003788 Neoplasm Micrometastasis Diseases 0.000 description 3
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 210000004907 gland Anatomy 0.000 description 3
- 238000003505 heat denaturation Methods 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 210000005228 liver tissue Anatomy 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000002751 oligonucleotide probe Substances 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- 239000001226 triphosphate Substances 0.000 description 3
- 235000011178 triphosphate Nutrition 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical group OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 2
- 206010006220 Breast cyst Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical group OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 2
- 102100031780 Endonuclease Human genes 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 101710103262 Glandular kallikrein Proteins 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 101710176220 Kallikrein-2 Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 102100035703 Prostatic acid phosphatase Human genes 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 108091081021 Sense strand Proteins 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 229920004892 Triton X-102 Polymers 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 238000002038 chemiluminescence detection Methods 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 238000002591 computed tomography Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 125000005647 linker group Chemical group 0.000 description 2
- 238000010841 mRNA extraction Methods 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 210000005075 mammary gland Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 208000010658 metastatic prostate carcinoma Diseases 0.000 description 2
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical class CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- 239000011824 nuclear material Substances 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000002980 postoperative effect Effects 0.000 description 2
- 208000023958 prostate neoplasm Diseases 0.000 description 2
- 108010043671 prostatic acid phosphatase Proteins 0.000 description 2
- 239000003161 ribonuclease inhibitor Substances 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000005382 thermal cycling Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- STGXGJRRAJKJRG-JDJSBBGDSA-N (3r,4r,5r)-5-(hydroxymethyl)-3-methoxyoxolane-2,4-diol Chemical compound CO[C@H]1C(O)O[C@H](CO)[C@H]1O STGXGJRRAJKJRG-JDJSBBGDSA-N 0.000 description 1
- MEOVPKDOYAIVHZ-UHFFFAOYSA-N 2-chloro-1-(1-methylpyrrol-2-yl)ethanol Chemical compound CN1C=CC=C1C(O)CCl MEOVPKDOYAIVHZ-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 102100022524 Alpha-1-antichymotrypsin Human genes 0.000 description 1
- 239000000592 Artificial Cell Substances 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 1
- 208000007433 Lymphatic Metastasis Diseases 0.000 description 1
- 208000004059 Male Breast Neoplasms Diseases 0.000 description 1
- 206010027452 Metastases to bone Diseases 0.000 description 1
- 206010027459 Metastases to lymph nodes Diseases 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241000713869 Moloney murine leukemia virus Species 0.000 description 1
- 206010029260 Neuroblastoma Diseases 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 108020003217 Nuclear RNA Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 208000006994 Precancerous Conditions Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- YZCKVEUIGOORGS-IGMARMGPSA-N Protium Chemical compound [1H] YZCKVEUIGOORGS-IGMARMGPSA-N 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 102000012479 Serine Proteases Human genes 0.000 description 1
- 108010022999 Serine Proteases Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical class OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
- 108700022175 Tissue Kallikreins Proteins 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- HAXFWIACAGNFHA-UHFFFAOYSA-N aldrithiol Chemical compound C=1C=CC=NC=1SSC1=CC=CC=N1 HAXFWIACAGNFHA-UHFFFAOYSA-N 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 108010091628 alpha 1-Antichymotrypsin Proteins 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 201000007295 breast benign neoplasm Diseases 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 210000002726 cyst fluid Anatomy 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 239000005549 deoxyribonucleoside Substances 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- IWBOPFCKHIJFMS-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl) ether Chemical compound NCCOCCOCCN IWBOPFCKHIJFMS-UHFFFAOYSA-N 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 201000007741 female breast cancer Diseases 0.000 description 1
- 201000002276 female breast carcinoma Diseases 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 208000013210 hematogenous Diseases 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 102000046689 human FOLH1 Human genes 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- IZWSFJTYBVKZNK-UHFFFAOYSA-N lauryl sulfobetaine Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O IZWSFJTYBVKZNK-UHFFFAOYSA-N 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 229910003002 lithium salt Inorganic materials 0.000 description 1
- 159000000002 lithium salts Chemical class 0.000 description 1
- WAHQBNXSPALNEA-UHFFFAOYSA-L lithium succinate Chemical compound [Li+].[Li+].[O-]C(=O)CCC([O-])=O WAHQBNXSPALNEA-UHFFFAOYSA-L 0.000 description 1
- 229960004254 lithium succinate Drugs 0.000 description 1
- YFVGRULMIQXYNE-UHFFFAOYSA-M lithium;dodecyl sulfate Chemical compound [Li+].CCCCCCCCCCCCOS([O-])(=O)=O YFVGRULMIQXYNE-UHFFFAOYSA-M 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 210000005265 lung cell Anatomy 0.000 description 1
- 238000002595 magnetic resonance imaging Methods 0.000 description 1
- 201000003175 male breast cancer Diseases 0.000 description 1
- 208000010907 male breast carcinoma Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- UYDLBVPAAFVANX-UHFFFAOYSA-N octylphenoxy polyethoxyethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCO)C=C1 UYDLBVPAAFVANX-UHFFFAOYSA-N 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 210000004976 peripheral blood cell Anatomy 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- MWWATHDPGQKSAR-UHFFFAOYSA-N propyne Chemical group CC#C MWWATHDPGQKSAR-UHFFFAOYSA-N 0.000 description 1
- 201000005825 prostate adenocarcinoma Diseases 0.000 description 1
- 210000005267 prostate cell Anatomy 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 239000002342 ribonucleoside Substances 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 210000001625 seminal vesicle Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 239000000439 tumor marker Substances 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 201000010653 vesiculitis Diseases 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000004246 zinc acetate Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Hospice & Palliative Care (AREA)
- Biophysics (AREA)
- Oncology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11764099P | 1999-01-28 | 1999-01-28 | |
| US60/117,640 | 1999-01-28 | ||
| PCT/US2000/002270 WO2000044940A2 (en) | 1999-01-28 | 2000-01-28 | Nucleic acid sequences for detecting genetic markers for cancer in a biological sample |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002535014A JP2002535014A (ja) | 2002-10-22 |
| JP2002535014A5 JP2002535014A5 (enExample) | 2007-03-15 |
| JP4495349B2 true JP4495349B2 (ja) | 2010-07-07 |
Family
ID=22374015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000596180A Expired - Fee Related JP4495349B2 (ja) | 1999-01-28 | 2000-01-28 | 癌の遺伝マーカーを生物学的サンプルにおいて検出するための核酸配列 |
Country Status (6)
| Country | Link |
|---|---|
| US (5) | US6551778B1 (enExample) |
| EP (1) | EP1151142A2 (enExample) |
| JP (1) | JP4495349B2 (enExample) |
| AU (1) | AU767587B2 (enExample) |
| CA (1) | CA2360073C (enExample) |
| WO (1) | WO2000044940A2 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2324503T3 (es) | 1997-04-10 | 2009-08-07 | Stichting Katholieke Universiteit University Medical Centre Nijmegen | Pca3, genes pca3 y metodos de uso. |
| EP1151142A2 (en) * | 1999-01-28 | 2001-11-07 | Gen-Probe Incorporated | Nucleic acid sequences for detecting genetic markers for cancer in a biological sample |
| JP2003510075A (ja) * | 1999-09-29 | 2003-03-18 | ディグノキュアー・インコーポレーテッド | 良性および悪性の前立腺組織におけるpca3メッセンジャーrna種 |
| AU2002217991A1 (en) * | 2000-11-21 | 2002-06-03 | Diadexus, Inc. | Compositions and methods relating to prostate specific genes and proteins |
| WO2003068918A2 (en) * | 2002-02-11 | 2003-08-21 | Auburn University | High-sensitivity real-time polymerase chain reaction for detection of nucleic acids |
| JP4824540B2 (ja) | 2003-02-07 | 2011-11-30 | ダイアノキュアー インク. | サンプル中の前立腺癌を検出する方法 |
| ES2321131T3 (es) * | 2003-05-01 | 2009-06-02 | Veridex Llc | Ensayo intraoperativo de ganglios linfaticos. |
| CA2491067A1 (en) | 2004-12-24 | 2006-06-24 | Stichting Katholieke Universiteit | Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer |
| US20070130694A1 (en) * | 2005-12-12 | 2007-06-14 | Michaels Emily W | Textile surface modification composition |
| WO2008080029A2 (en) | 2006-12-21 | 2008-07-03 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| US20110091883A1 (en) * | 2009-02-18 | 2011-04-21 | Helicos Biosciences Corporation | Methods for analyzing minute cellular nucleic acids |
| CN102414554B (zh) * | 2009-04-22 | 2014-11-26 | 威斯康星校友研究基金会 | 采用液晶的分析物检测 |
| EP2449132B1 (en) | 2009-07-01 | 2015-05-13 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
| MX338883B (es) * | 2010-07-27 | 2016-05-04 | Genomic Health Inc | Metodo para usar expresion de gen para determinar el pronostico de cancer de prostata. |
| EP2616555B1 (en) | 2010-09-16 | 2017-11-08 | Gen-Probe Incorporated | Capture probes immobilizable via l-nucleotide tail |
| US9255293B2 (en) | 2010-11-01 | 2016-02-09 | Gen-Probe Incorporated | Integrated capture and amplification of target nucleic acid for sequencing |
| EP2809807B1 (en) | 2012-02-01 | 2019-04-10 | Gen-Probe Incorporated | Asymmetric hairpin target capture oligomers |
| EP3312749B1 (en) | 2012-03-05 | 2024-05-01 | OY Arctic Partners AB | Methods and apparatuses for predicting risk of prostate cancer and prostate gland volume |
| US12326453B2 (en) | 2014-03-28 | 2025-06-10 | Opko Diagnostics, Llc | Compositions and methods for active surveillance of prostate cancer |
| FI3123381T3 (fi) | 2014-03-28 | 2023-11-27 | Opko Diagnostics Llc | Eturauhassyövän diagnosointiin liittyviä koostumuksia ja menetelmiä |
| CA2979559A1 (en) | 2015-03-27 | 2016-10-06 | Opko Diagnostics, Llc | Prostate antigen standards and uses thereof |
| EP3448998B1 (en) * | 2016-04-27 | 2020-06-03 | Gen-Probe Incorporated | Blood cell lysis reagent |
| CN111518170A (zh) * | 2020-05-09 | 2020-08-11 | 新乡医学院 | 基于fret的psa荧光探针、制备方法及其应用 |
| CA3254118A1 (en) | 2022-03-15 | 2023-09-21 | Diagenode S A | DETECTION OF THE METHYLATION STATE OF A DNA SAMPLE |
Family Cites Families (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3725010A (en) * | 1971-08-23 | 1973-04-03 | Beckman Instruments Inc | Apparatus for automatically performing chemical processes |
| US5283174A (en) | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| US5851764A (en) * | 1990-10-25 | 1998-12-22 | The Trustees Of Columbia University In The City Of New York | Human prostate tumor inducing gene-1 and uses thereof |
| AU662906B2 (en) | 1991-06-26 | 1995-09-21 | F. Hoffmann-La Roche Ag | Methods for detection of carcinoma metastases by nucleic acid amplification |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| US5786148A (en) | 1996-11-05 | 1998-07-28 | Incyte Pharmaceuticals, Inc. | Polynucleotides encoding a novel prostate-specific kallikrein |
| WO1994010343A1 (en) | 1992-10-29 | 1994-05-11 | Thomas Jefferson University | Methods of detecting micrometastasis of prostate cancer |
| US5674682A (en) * | 1992-10-29 | 1997-10-07 | Thomas Jefferson University | Nucleic acid primers for detecting micrometastasis of prostate cancer |
| EP1757694A3 (en) | 1992-11-05 | 2008-02-27 | Sloan Kettering Institute For Cancer Research | Prostate-specific membrane antigen |
| US5516639A (en) | 1993-07-22 | 1996-05-14 | Mayo Foundation For Medical Education And Research | Antibodies specific for human prostate glandular kallkrein |
| US6103237A (en) * | 1993-07-22 | 2000-08-15 | Hybritech Incorporated | Stable variant hK2 polypeptide |
| US6569432B1 (en) * | 1995-02-24 | 2003-05-27 | Sloan-Kettering Institute For Cancer Research | Prostate-specific membrane antigen and uses thereof |
| FR2714062B1 (fr) * | 1993-12-22 | 1996-02-16 | Bio Merieux | Promoteur modifié pour ARN polymérase, sa préparation et ses applications. |
| US5599677A (en) | 1993-12-29 | 1997-02-04 | Abbott Laboratories | Immunoassays for prostate specific antigen |
| EP0760006A1 (en) | 1994-04-15 | 1997-03-05 | The Trustees Of Columbia University In The City Of New York | Method for molecular staging of prostate cancer |
| JPH10500294A (ja) | 1994-05-10 | 1998-01-13 | マヨ ファウンデーション フォー メディカル エデュケーション アンド リサーチ | 組換え型hk2ポリペプチド |
| AU4897896A (en) * | 1995-01-04 | 1996-07-24 | Trustees Of Boston University | Primers for the pcr amplification of metastatic sequences |
| US5698402A (en) | 1995-02-23 | 1997-12-16 | Dianon Systems, Inc. | Methods for diagnosing benign prostatic hyperplasia |
| US7037647B1 (en) * | 1995-02-24 | 2006-05-02 | Sloan-Kettering Institute For Cancer Research | Prostate-specific membrane antigen and uses thereof |
| US5773292A (en) | 1995-06-05 | 1998-06-30 | Cornell University | Antibodies binding portions, and probes recognizing an antigen of prostate epithelial cells but not antigens circulating in the blood |
| GB2302874B (en) * | 1995-06-29 | 1999-11-10 | Orion Yhtymae Oy | Novel proteins |
| WO1997007242A1 (en) | 1995-08-16 | 1997-02-27 | Steven Lehrer | Method for detecting circulating breast cancer cells |
| US5710007A (en) | 1995-09-29 | 1998-01-20 | Luderer; Albert A. | Methods for diagnosing prostatic adenocarcinoma |
| US5861248A (en) | 1996-03-29 | 1999-01-19 | Urocor, Inc. | Biomarkers for detection of prostate cancer |
| EP0891550A1 (en) | 1996-04-05 | 1999-01-20 | The Johns Hopkins University School Of Medicine | A method of enriching rare cells |
| AU2676197A (en) | 1996-04-16 | 1997-11-07 | Smithkline Beecham Corporation | A method for detection of prostate specific antigen used in monitoring and diagnosis of prostate cancer |
| US5858673A (en) * | 1996-06-24 | 1999-01-12 | Charlotte-Mecklenburg Hospital Authority | Method for detecting prostate cells |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| WO1998021365A2 (en) | 1996-11-14 | 1998-05-22 | Mayo Foundation For Medical Education And Research | Method for detection of metastatic prostate cancer |
| US6479263B1 (en) | 1996-11-14 | 2002-11-12 | Baylor College Of Medicine | Method for detection of micrometastatic prostate cancer |
| CA2218754A1 (en) | 1996-11-15 | 1998-05-15 | Eli Lilly And Company | Recombinant psa expression vectors and assays using the same |
| US6100444A (en) | 1997-02-11 | 2000-08-08 | University Of Rochester Medical Center | Prostate specific regulatory nucleic acid sequences and transgenic non-human animals expressing prostate specific antigen |
| US6630305B1 (en) | 1999-11-12 | 2003-10-07 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of prostate cancer |
| US7033827B2 (en) * | 1997-02-25 | 2006-04-25 | Corixa Corporation | Prostate-specific polynucleotide compositions |
| US6759515B1 (en) | 1997-02-25 | 2004-07-06 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of prostate cancer |
| US6620922B1 (en) | 1997-02-25 | 2003-09-16 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of prostate cancer |
| WO1998040513A1 (en) | 1997-03-11 | 1998-09-17 | Ferrari Anna C | Method of detecting prostate cancer metastasis |
| WO1998046788A2 (en) * | 1997-04-11 | 1998-10-22 | Micromet Gesellschaft Für Biomedizinische Forschung Mbh | Primers and methods for the detection of disseminated tumor cells |
| ES2312186T3 (es) | 1997-04-30 | 2009-02-16 | Hybritech Incorporated | Formas del antigeno prostatico especifico y procedimientos para su deteccion. |
| AU8259798A (en) * | 1997-06-20 | 1999-01-04 | George G Klee | Method for detection of breast cancer |
| US5817798A (en) * | 1997-09-17 | 1998-10-06 | Abbott Laboratories | Rapid RNA isolation procedure in the presence of a transition metal ion |
| US20030215803A1 (en) * | 2000-12-07 | 2003-11-20 | Garcia Pablo Dominguez | Human genes and gene expression products isolated from human prostate |
| US8101349B2 (en) * | 1997-12-23 | 2012-01-24 | Novartis Vaccines And Diagnostics, Inc. | Gene products differentially expressed in cancerous cells and their methods of use II |
| JP2000069969A (ja) | 1998-08-28 | 2000-03-07 | Hitachi Chem Co Ltd | プライマーDNA及びこれを用いる前立腺特異抗原をコードするmRNAの検出方法 |
| EP1151142A2 (en) * | 1999-01-28 | 2001-11-07 | Gen-Probe Incorporated | Nucleic acid sequences for detecting genetic markers for cancer in a biological sample |
| WO2000049158A2 (en) * | 1999-02-18 | 2000-08-24 | Compugen Ltd. | Psa and klk-2 splicing variants |
| CA2362885A1 (en) | 1999-03-11 | 2000-09-14 | Mount Sinai Hospital | Human kallikrein-like genes |
| WO2000065067A2 (en) | 1999-04-23 | 2000-11-02 | University Of Washington | Prostate-specific polynucleotides, polypeptides and their methods of use |
| AU5416700A (en) | 1999-05-20 | 2000-12-12 | Fahri Saatcioglu | Differentially expressed genes in prostate cancer |
| WO2001060860A2 (en) * | 2000-02-17 | 2001-08-23 | Millennium Predictive Medicine, Inc. | Genes differentially expressed in human prostate cancer and their use |
| EP2253717B1 (en) | 2000-04-07 | 2014-04-30 | Eiken Kagaku Kabushiki Kaisha | Method of amplifying nucleic acid by using double-stranded nucleic acid as template |
| US20020132976A1 (en) | 2000-04-10 | 2002-09-19 | Artur Pedyczak | Immunogenic peptides derived from prostate-specific antigen (PSA) and uses thereof |
| ATE325871T1 (de) | 2000-05-01 | 2006-06-15 | Eiken Chemical | Verfahren zur erkennung des produkts einer nukleinsäuresynthetisierungsreaktion |
| CN1283807C (zh) * | 2001-06-18 | 2006-11-08 | 荣研化学株式会社 | 有效检测双链核酸的方法 |
| US20050112705A1 (en) * | 2002-03-14 | 2005-05-26 | Laurent Bracco | Variants of human kallikrein-2 and kallikrein-3 and uses thereof |
| US7348142B2 (en) * | 2002-03-29 | 2008-03-25 | Veridex, Lcc | Cancer diagnostic panel |
| JP4824540B2 (ja) | 2003-02-07 | 2011-11-30 | ダイアノキュアー インク. | サンプル中の前立腺癌を検出する方法 |
| US20040223885A1 (en) * | 2003-05-06 | 2004-11-11 | Keen Randy E. | Apparatus for the automated synthesis of polynucleotides |
| US20060008468A1 (en) * | 2004-06-17 | 2006-01-12 | Chih-Sheng Chiang | Combinations of tumor-associated antigens in diagnostics for various types of cancers |
-
2000
- 2000-01-28 EP EP00913284A patent/EP1151142A2/en not_active Withdrawn
- 2000-01-28 US US09/493,491 patent/US6551778B1/en not_active Expired - Lifetime
- 2000-01-28 CA CA2360073A patent/CA2360073C/en not_active Expired - Fee Related
- 2000-01-28 JP JP2000596180A patent/JP4495349B2/ja not_active Expired - Fee Related
- 2000-01-28 WO PCT/US2000/002270 patent/WO2000044940A2/en not_active Ceased
- 2000-01-28 AU AU34755/00A patent/AU767587B2/en not_active Ceased
-
2002
- 2002-10-17 US US10/273,707 patent/US6811985B2/en not_active Expired - Lifetime
-
2004
- 2004-10-28 US US10/978,145 patent/US7267956B2/en not_active Expired - Lifetime
-
2007
- 2007-08-13 US US11/838,168 patent/US20080026398A1/en not_active Abandoned
-
2008
- 2008-02-14 US US12/031,489 patent/US20090035773A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20090035773A1 (en) | 2009-02-05 |
| US20080026398A1 (en) | 2008-01-31 |
| CA2360073A1 (en) | 2000-08-03 |
| WO2000044940A3 (en) | 2000-12-07 |
| JP2002535014A (ja) | 2002-10-22 |
| US20030104448A1 (en) | 2003-06-05 |
| WO2000044940A2 (en) | 2000-08-03 |
| US7267956B2 (en) | 2007-09-11 |
| US6551778B1 (en) | 2003-04-22 |
| CA2360073C (en) | 2011-01-18 |
| AU767587B2 (en) | 2003-11-20 |
| EP1151142A2 (en) | 2001-11-07 |
| US20050118630A1 (en) | 2005-06-02 |
| US6811985B2 (en) | 2004-11-02 |
| AU3475500A (en) | 2000-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4495349B2 (ja) | 癌の遺伝マーカーを生物学的サンプルにおいて検出するための核酸配列 | |
| US11104958B2 (en) | Method to detect prostate cancer in a sample | |
| JP4944041B2 (ja) | 前立腺癌の予後判定用マーカー及び/又はテラノスティックマーカーとなる、尿沈渣及び/又は尿のmRNA比 | |
| US20090226925A1 (en) | Methods for Detecting Circulating Tumor Cells | |
| EP0760006A1 (en) | Method for molecular staging of prostate cancer | |
| CA2432365A1 (en) | Specific method of prostate cancer detection based on pca3, and kits therefore | |
| US12410424B2 (en) | Diagnosis of prostate cancer | |
| JP4315812B2 (ja) | 癌腫の分子診断および予後 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20060912 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070124 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20090708 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20090713 |
|
| RD02 | Notification of acceptance of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7422 Effective date: 20090713 |
|
| RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20090714 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20090928 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20091026 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100215 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20100305 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20100402 |
|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20100409 |
|
| R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130416 Year of fee payment: 3 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130416 Year of fee payment: 3 |
|
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140416 Year of fee payment: 4 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |