JP4416400B2 - 抗原特異的ｔ−細胞の機能的精製のための可逆的ｍｈｃ多量体染色法 - Google Patents
抗原特異的ｔ−細胞の機能的精製のための可逆的ｍｈｃ多量体染色法 Download PDFInfo
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- A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
- A61K40/45—Bacterial antigens
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- A61K40/00—Cellular immunotherapy
- A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
- A61K40/46—Viral antigens
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5047—Cells of the immune system
- G01N33/505—Cells of the immune system involving T-cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
- A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
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- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
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- Food Science & Technology (AREA)
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- Wood Science & Technology (AREA)
- Ecology (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00128634A EP1227321A1 (en) | 2000-12-28 | 2000-12-28 | Reversible MHC multimer staining for functional purification of antigen-specific T cells |
| PCT/EP2001/015353 WO2002054065A2 (en) | 2000-12-28 | 2001-12-28 | Reversible mhc multimer staining for functional purification of antigen-specific t cells |
Publications (3)
| Publication Number | Publication Date |
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| JP2004525354A JP2004525354A (ja) | 2004-08-19 |
| JP2004525354A5 JP2004525354A5 (https=) | 2008-12-18 |
| JP4416400B2 true JP4416400B2 (ja) | 2010-02-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2002554713A Expired - Lifetime JP4416400B2 (ja) | 2000-12-28 | 2001-12-28 | 抗原特異的ｔ−細胞の機能的精製のための可逆的ｍｈｃ多量体染色法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US7776562B2 (https=) |
| EP (2) | EP1227321A1 (https=) |
| JP (1) | JP4416400B2 (https=) |
| CA (1) | CA2467434C (https=) |
| WO (1) | WO2002054065A2 (https=) |
Families Citing this family (84)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040067532A1 (en) | 2002-08-12 | 2004-04-08 | Genetastix Corporation | High throughput generation and affinity maturation of humanized antibody |
| US20090061478A1 (en) * | 2006-01-30 | 2009-03-05 | Lene Have Poulsen | High-Speed Quantification of Antigen Specific T-Cells in Whole Blood by Flow Cytometry |
| EP1878744A1 (en) * | 2006-07-13 | 2008-01-16 | Max-Delbrück-Centrum für Molekulare Medizin (MDC) | Epitope-tag for surface-expressed T-cell receptor proteins, uses thereof and method of selecting host cells expressing them |
| AU2007353319A1 (en) | 2006-11-15 | 2008-11-20 | Invitrogen Dynal As | Methods for reversibly binding a biotin compound to a support |
| WO2008116468A2 (en) | 2007-03-26 | 2008-10-02 | Dako Denmark A/S | Mhc peptide complexes and uses thereof in infectious diseases |
| EP3620465B1 (en) | 2007-07-03 | 2025-02-19 | Dako Denmark A/S | Improved methods for generation, labeling and use of mhc multimers |
| EP3124497B1 (en) | 2007-09-14 | 2020-04-15 | Adimab, LLC | Rationally designed, synthetic antibody libraries and uses therefor |
| US12529164B2 (en) | 2007-09-14 | 2026-01-20 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
| US8877688B2 (en) | 2007-09-14 | 2014-11-04 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
| US10611818B2 (en) | 2007-09-27 | 2020-04-07 | Agilent Technologies, Inc. | MHC multimers in tuberculosis diagnostics, vaccine and therapeutics |
| DK2254592T3 (da) | 2008-02-28 | 2019-09-09 | Dako Denmark As | MHC-multimerer til Borrelia-diagnostik og sygdom |
| WO2010009735A2 (en) | 2008-07-23 | 2010-01-28 | Dako Denmark A/S | Combinatorial analysis and repair |
| GB0817244D0 (en) | 2008-09-20 | 2008-10-29 | Univ Cardiff | Use of a protein kinase inhibitor to detect immune cells, such as T cells |
| US11992518B2 (en) | 2008-10-02 | 2024-05-28 | Agilent Technologies, Inc. | Molecular vaccines for infectious disease |
| WO2010037402A1 (en) | 2008-10-02 | 2010-04-08 | Dako Denmark A/S | Molecular vaccines for infectious disease |
| JP5686098B2 (ja) * | 2009-09-17 | 2015-03-18 | Jsr株式会社 | アビジンとビオチン誘導体の解離方法及び解離剤 |
| EP2567226B1 (en) | 2010-05-06 | 2016-08-10 | Adaptive Biotechnologies Corporation | Monitoring health and disease status using clonotype profiles |
| CN103124740B (zh) * | 2010-09-21 | 2015-09-02 | 美国卫生和人力服务部 | 抗-ssx-2t细胞受体和相关材料及使用方法 |
| WO2012044999A2 (en) * | 2010-10-01 | 2012-04-05 | Ludwig Institute For Cancer Research Ltd. | Reversible protein multimers, methods for their production and use |
| US9023604B2 (en) | 2011-07-18 | 2015-05-05 | Iba Gmbh | Method of reversibly staining a target cell |
| WO2013056090A1 (en) * | 2011-10-12 | 2013-04-18 | University Of Connecticut | Affinity-based materials for the non-destructive separation and recovery of cells |
| ES3011307T3 (en) * | 2012-02-23 | 2025-04-07 | Juno Therapeutics Gmbh | Chromatographic isolation of cells and other complex biological materials |
| CN102662054B (zh) * | 2012-05-10 | 2014-08-13 | 东南大学 | 一种同步检测特异性胸腺依赖性淋巴细胞数量和功能的方法 |
| CN105073770B (zh) | 2012-11-16 | 2019-08-06 | Iba股份有限公司 | 链霉亲和素突变蛋白及其使用方法 |
| NZ759969A (en) | 2013-12-20 | 2022-12-23 | Fred Hutchinson Cancer Center | Tagged chimeric effector molecules and receptors thereof |
| US9970849B2 (en) | 2014-01-21 | 2018-05-15 | Stemcell Technologies Inc. | Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface |
| SI3132247T1 (sl) | 2014-04-16 | 2021-12-31 | Juno Therapeutics Gmbh | Postopki, kompleti in naprava za povečanje populacije celic |
| CA2946312A1 (en) | 2014-04-23 | 2015-10-29 | Juno Therapeutics, Inc. | Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy |
| US11150239B2 (en) | 2014-04-30 | 2021-10-19 | Iba Lifesciences Gmbh | Method of isolating a target cell |
| EP3037820A1 (en) * | 2014-12-27 | 2016-06-29 | Miltenyi Biotec GmbH | Cell detection methods and reagents having a releasable labelling moiety |
| WO2016166568A1 (en) | 2015-04-16 | 2016-10-20 | Juno Therapeutics Gmbh | Methods, kits and apparatus for expanding a population of cells |
| EP3288570A4 (en) | 2015-04-29 | 2018-11-21 | Fred Hutchinson Cancer Research Center | Modified stem cells and uses thereof |
| JP6657721B2 (ja) * | 2015-09-30 | 2020-03-04 | ソニー株式会社 | 細胞の分析方法、細胞分析用チップ、細胞分析用試薬、細胞分析用キット及び細胞分析用装置 |
| MX2018004875A (es) | 2015-10-22 | 2018-08-01 | Juno Therapeutics Gmbh | Metodos para cultivar celulas y kits y aparatos para ello. |
| AU2016341529B2 (en) * | 2015-10-22 | 2023-03-30 | Juno Therapeutics Gmbh | Methods for culturing cells and kits and apparatus for same |
| EP3365453A2 (en) | 2015-10-22 | 2018-08-29 | Juno Therapeutics GmbH | Methods, kits, agents and apparatuses for transduction |
| EP3443123B1 (en) | 2016-04-11 | 2021-08-11 | Board of Regents, The University of Texas System | Methods and compositions for detecting single t cell receptor affinity and sequence |
| EP3488011A1 (en) | 2016-06-28 | 2019-05-29 | Technische Universität München | Combination of reversible and irreversible cell labeling for analizing receptor-ligand koff rate |
| TWI795365B (zh) | 2016-08-11 | 2023-03-11 | 荷蘭商凱特製藥歐洲公司 | 製造和使用可溶性主要組織相容性複合體（ｍｈｃ）分子類之方法 |
| US10294454B2 (en) | 2016-08-24 | 2019-05-21 | General Electric Company | Methods and kits for cell activation |
| EP3336546B1 (en) | 2016-12-13 | 2020-07-01 | Miltenyi Biotec B.V. & Co. KG | Reversible cell labelling with conjugates having two releasable binding sites |
| EP3336545A1 (en) | 2016-12-13 | 2018-06-20 | Miltenyi Biotec GmbH | Reversible labeling of antigens in biological specimens |
| CA3050085A1 (en) | 2017-01-20 | 2018-07-26 | Juno Therapeutics Gmbh | Cell surface conjugates and related cell compositions and methods |
| JP7339160B2 (ja) | 2017-04-27 | 2023-09-05 | ジュノ セラピューティクス ゲーエムベーハー | オリゴマー粒子試薬およびその使用方法 |
| SG11202000606TA (en) | 2017-07-29 | 2020-02-27 | Juno Therapeutics Inc | Reagents for expanding cells expressing recombinant receptors |
| EP3489684B1 (en) | 2017-11-27 | 2024-06-26 | Miltenyi Biotec B.V. & Co. KG | Method for photobleaching stained cells |
| CN119193493A (zh) | 2017-12-08 | 2024-12-27 | 朱诺治疗学股份有限公司 | 生产工程化t细胞组合物的过程 |
| CN116148467A (zh) * | 2017-12-12 | 2023-05-23 | 清华大学 | 检测翻译后修饰蛋白质与其配体间相互作用的成套试剂 |
| US12516099B2 (en) | 2018-08-09 | 2026-01-06 | Juno Therapeutics, Inc. | Processes for generating engineered cells and compositions thereof |
| US20210246189A1 (en) | 2018-09-03 | 2021-08-12 | Technische Universitaet Muenchen | A double peptide tag combining reversibility and flexible functionalization |
| SG11202102108QA (en) | 2018-09-11 | 2021-04-29 | Juno Therapeutics Inc | Methods for mass spectrometry analysis of engineered cell compositions |
| SG11202104355SA (en) | 2018-10-31 | 2021-05-28 | Juno Therapeutics Gmbh | Methods for selection and stimulation of cells and apparatus for same |
| ES2968737T3 (es) | 2018-11-06 | 2024-05-13 | Juno Therapeutics Inc | Proceso para producir células T manipuladas genéticamente |
| WO2020127222A2 (en) | 2018-12-17 | 2020-06-25 | Immudex Aps | Panel comprising borrelia mhc multimers |
| CA3136348A1 (en) * | 2019-04-09 | 2020-10-15 | Nurix Therapeutics, Inc. | 3-substituted piperidine compounds for cbl-b inhibition, and use of a cbl-b inhibitor in combination with a cancer vaccine and/or oncolytic virus |
| WO2020207963A1 (en) | 2019-04-12 | 2020-10-15 | Miltenyi Biotec B.V. & Co. KG | Conjugates having an enzymatically releasable detection moiety and a barcode moiety |
| AU2020278592B2 (en) | 2019-05-17 | 2024-10-10 | Nurix Therapeutics, Inc. | Cyano cyclobutyl compounds for Cbl-b inhibition and uses thereof |
| CA3142108A1 (en) | 2019-05-28 | 2020-12-03 | Miltenyi Biotec B.V. & Co. KG | Method for generation of genetically modified t cells |
| CN114207136A (zh) | 2019-06-07 | 2022-03-18 | 朱诺治疗学股份有限公司 | 自动化t细胞培养 |
| MX2021015675A (es) | 2019-06-26 | 2022-02-03 | Nurix Therapeutics Inc | Compuestos de bencil-triazol sustituidos para la inhibicion de cbl-b y otros usos de los mismos. |
| AU2020320195A1 (en) | 2019-07-30 | 2022-03-10 | Nurix Therapeutics, Inc. | Urea, amide, and substituted heteroaryl compounds for Cbl-b inhibition |
| KR20220073738A (ko) | 2019-08-30 | 2022-06-03 | 주노 쎄러퓨티크스 인코퍼레이티드 | 세포 분류를 위한 기계 학습 방법 |
| WO2021047804A1 (en) | 2019-09-11 | 2021-03-18 | Miltenyi Biotec B.V. & Co. KG | In vitro method for transduction of t cells in the presence of malignant cells |
| KR20220101641A (ko) | 2019-10-30 | 2022-07-19 | 주노 테라퓨틱스 게엠베하 | 세포 선택 및/또는 자극 장치 및 사용 방법 |
| EP4078178B1 (en) | 2019-12-20 | 2024-10-23 | Miltenyi Biotec B.V. & Co. KG | Reversible cell detection via mhc with conjugates having an enzymatically cleavable detection moiety |
| JP2023512209A (ja) | 2020-01-28 | 2023-03-24 | ジュノー セラピューティクス インコーポレイテッド | Ｔ細胞形質導入のための方法 |
| WO2021152178A1 (en) | 2020-01-31 | 2021-08-05 | Cell.Copedia GmbH | Methods of isolating a biological entity |
| IL295384B1 (en) | 2020-02-12 | 2026-04-01 | Juno Therapeutics Inc | T-cell preparations with chimeric antigen receptor directed against CD19 and methods and uses thereof |
| IL295381B1 (en) | 2020-02-12 | 2026-04-01 | Juno Therapeutics Inc | BCMA-directed chimeric T-cell antigen receptor compounds and methods and uses thereof |
| CN115835873A (zh) | 2020-05-13 | 2023-03-21 | 朱诺治疗学股份有限公司 | 用于产生表达重组受体的供体分批细胞的方法 |
| EP3916393B1 (en) | 2020-05-27 | 2023-01-25 | Miltenyi Biotec B.V. & Co. KG | Method for detection of cells by repetitive staining and destaining |
| EP4204809A1 (en) | 2020-08-27 | 2023-07-05 | Miltenyi Biotec B.V. & Co. KG | Releasable reagents for cell separation |
| WO2022048780A1 (en) | 2020-09-07 | 2022-03-10 | Miltenyi Biotec B.V. & Co. KG | Conjugates having an enzymatically releasable detection moiety and a barcode moiety |
| JP2024517863A (ja) | 2021-05-06 | 2024-04-23 | ジュノ・セラピューティクス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | 細胞を刺激し、形質導入する方法 |
| WO2023017115A1 (en) | 2021-08-11 | 2023-02-16 | Iba Lifesciences Gmbh | Fractionation of cells based on a marker protein |
| EP4227423A1 (en) | 2022-02-09 | 2023-08-16 | Technische Universität München | Means and methods for identifying high affinity t-cells |
| US20250297282A1 (en) | 2022-05-05 | 2025-09-25 | Juno Therapeutics Gmbh | Viral-binding protein and related reagents, articles, and methods of use |
| EP4532695A1 (en) | 2022-05-25 | 2025-04-09 | Celgene Corporation | Methods of manufacturing t cell therapies |
| WO2024100604A1 (en) | 2022-11-09 | 2024-05-16 | Juno Therapeutics Gmbh | Methods for manufacturing engineered immune cells |
| WO2024124132A1 (en) | 2022-12-09 | 2024-06-13 | Juno Therapeutics, Inc. | Machine learning methods for predicting cell phenotype using holographic imaging |
| JP2026504491A (ja) | 2023-02-03 | 2026-02-05 | ツェー３エス２ ゲーエムベーハー | 操作された免疫細胞の非ウイルス的製造のための方法 |
| CN121127253A (zh) | 2023-02-28 | 2025-12-12 | 朱诺治疗学股份有限公司 | 治疗系统性自身免疫性疾病的细胞疗法 |
| EP4484441A1 (en) | 2023-06-26 | 2025-01-01 | Eberhard-Karls-Universität Tübingen | Mhc-class i derived polypeptide |
| WO2025083196A1 (en) | 2023-10-18 | 2025-04-24 | Tq Therapeutics Gmbh | A method of manufacturing a cellular therapy product and respective kits and devices |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4948590A (en) * | 1987-06-09 | 1990-08-14 | Yale University | Avidin or streptavidin conjugated liposomes |
| DE4237113B4 (de) * | 1992-11-03 | 2006-10-12 | "Iba Gmbh" | Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins |
| US5595881A (en) | 1994-08-09 | 1997-01-21 | Anergen, Inc. | Method for the detection of antigen presenting cells |
| US6022951A (en) * | 1995-04-11 | 2000-02-08 | Univ Boston | Streptavidin mutants |
| US5998588A (en) * | 1995-09-01 | 1999-12-07 | University Of Washington | Interactive molecular conjugates |
| DE19641876B4 (de) * | 1996-10-10 | 2011-09-29 | Iba Gmbh | Streptavidinmuteine |
| US5985658A (en) * | 1997-11-14 | 1999-11-16 | Health Research Incorporated | Calmodulin-based cell separation technique |
| FR2841905B1 (fr) | 2002-07-05 | 2004-09-03 | Centre Nat Rech Scient | Fragments fab mutants de l'anticorps anti-cd4 chimere 13b8.2 et leurs applications |
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| US8298782B2 (en) | 2012-10-30 |
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| US20110070605A1 (en) | 2011-03-24 |
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| US20040082012A1 (en) | 2004-04-29 |
| EP1346217B1 (en) | 2020-10-21 |
| EP1346217A2 (en) | 2003-09-24 |
| WO2002054065A3 (en) | 2002-11-28 |
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