JP4349545B2 - Ｆｙ７ポリメラーゼ - Google Patents

Ｆｙ７ポリメラーゼ Download PDF

Info

Publication number
JP4349545B2
JP4349545B2 JP2000554763A JP2000554763A JP4349545B2 JP 4349545 B2 JP4349545 B2 JP 4349545B2 JP 2000554763 A JP2000554763 A JP 2000554763A JP 2000554763 A JP2000554763 A JP 2000554763A JP 4349545 B2 JP4349545 B2 JP 4349545B2
Authority
JP
Japan
Prior art keywords
dna
polymerase
dna polymerase
sequencing
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2000554763A
Other languages
English (en)
Japanese (ja)
Other versions
JP2002517997A (ja
JP2002517997A5 (enExample
Inventor
マリア・デイビス
カール・ダブリュー・フラー
ジョゼフ・エイ・マモーン
フアン・リン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Publication of JP2002517997A publication Critical patent/JP2002517997A/ja
Publication of JP2002517997A5 publication Critical patent/JP2002517997A5/ja
Application granted granted Critical
Publication of JP4349545B2 publication Critical patent/JP4349545B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
JP2000554763A 1998-06-17 1999-06-17 Ｆｙ７ポリメラーゼ Expired - Fee Related JP4349545B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8955698P 1998-06-17 1998-06-17
US60/089,556 1998-06-17
PCT/US1999/013741 WO1999065938A2 (en) 1998-06-17 1999-06-17 Fy7 polymerase

Publications (3)

Publication Number Publication Date
JP2002517997A JP2002517997A (ja) 2002-06-25
JP2002517997A5 JP2002517997A5 (enExample) 2006-08-10
JP4349545B2 true JP4349545B2 (ja) 2009-10-21

Family

ID=22218300

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000554763A Expired - Fee Related JP4349545B2 (ja) 1998-06-17 1999-06-17 Ｆｙ７ポリメラーゼ

Country Status (9)

Country Link
US (1) US6479267B1 (enExample)
EP (1) EP1086209B1 (enExample)
JP (1) JP4349545B2 (enExample)
CN (1) CN1134538C (enExample)
AT (1) ATE371022T1 (enExample)
AU (1) AU768993B2 (enExample)
CA (1) CA2330735C (enExample)
DE (1) DE69936919T2 (enExample)
WO (1) WO1999065938A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
CA2440754A1 (en) 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
EP1404824A2 (en) 2001-07-06 2004-04-07 Amersham Biosciences Corp. Novel dna polymerases having amino acid substitutions and homologs thereof
AU2002347981A1 (en) * 2001-11-07 2003-05-19 Applera Corporation Universal nucleotides for nucleic acid analysis
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
DE602005027700D1 (de) 2004-05-25 2011-06-09 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
DE602005020011D1 (de) * 2004-06-10 2010-04-29 Ge Healthcare Bio Sciences Verfahren zur nukleinsäureanalyse
JP2008522628A (ja) * 2004-12-11 2008-07-03 サイトジェニックス， インコーポレイテッド 高品質核酸のセルフリー生合成及びその使用
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP3447141B1 (en) 2013-03-18 2020-08-05 PreAnalytiX GmbH Stabilisation of biological samples
GB201721053D0 (en) * 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
CN110093410A (zh) * 2019-05-21 2019-08-06 通用生物系统（安徽）有限公司 一种dna测序反应试剂及其制备方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
JP2531246B2 (ja) * 1988-08-26 1996-09-04 東洋紡績株式会社 耐熱性ｄｎａポリメラ―ゼおよびその製法
SG46627A1 (en) 1989-12-22 1998-02-20 Hoffmann La Roche Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5593840A (en) 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5610066A (en) 1993-12-10 1997-03-11 Amersham Life Science, Inc. Nucleic acid modifying proteins from Pyrococcus furiosus
WO1995030756A1 (en) 1994-04-18 1995-11-16 United States Biochemical Corporation Thermostable alkaline phosphatase of thermus thermophilus
WO1996038568A1 (en) * 1995-05-31 1996-12-05 Amersham Life Science, Inc. Thermostable dna polymerases
WO1997009451A1 (en) * 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
DE69613856T2 (de) 1995-12-15 2002-04-04 Amersham Pharmacia Biotech Inc., Piscataway Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
US6294325B1 (en) 1996-07-05 2001-09-25 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of thermostable multi genes and proteins and uses thereof

Also Published As

Publication number Publication date
DE69936919D1 (en) 2007-10-04
WO1999065938A9 (en) 2000-06-08
DE69936919T2 (de) 2008-05-15
ATE371022T1 (de) 2007-09-15
JP2002517997A (ja) 2002-06-25
EP1086209A2 (en) 2001-03-28
CN1134538C (zh) 2004-01-14
CA2330735A1 (en) 1999-12-23
WO1999065938A3 (en) 2000-04-06
AU4575799A (en) 2000-01-05
CN1313893A (zh) 2001-09-19
EP1086209B1 (en) 2007-08-22
US6479267B1 (en) 2002-11-12
CA2330735C (en) 2010-05-11
WO1999065938A2 (en) 1999-12-23
AU768993B2 (en) 2004-01-15

Similar Documents

Publication Publication Date Title
JP4349545B2 (ja) Ｆｙ７ポリメラーゼ
US9102924B2 (en) DNA polymerases and related methods
Takagi et al. Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR
CA1341512C (en) Methods for producing a t7-type dna polymerase
JP2907231B2 (ja) Ｐｈ１２９ｄｎａポリメラーゼおよび該ポリメラーゼをコードするｄｎａ断片を用いるイン・ビトロｄｎａ合成反応
US5545552A (en) Purified thermostable pyrococcus furiosus DNA polymerase I
JP2017029172A (ja) キメラｄｎａポリメラーゼ
CN107475216B (zh) 重组型耐热dna聚合酶及其应用
US5866395A (en) Purified thermostable pyrococcus furiosus DNA polymerase I
JP2002506626A (ja) Ｔｈｅｍｏａｎａｅｒｏｂａｃｔｅｒｔｈｅｒｍｏｈｙｄｒｏｓｕｌｆｒｉｃｕｓ由来の熱安定性ＤＮＡポリメラーゼ
CN113637085B (zh) 融合dna聚合酶突变体及其在等温扩增中的应用
Blöndal et al. Cloning, sequence analysis and functional characterization of DNA polymerase I from the thermophilic eubacterium Rhodothermus marinus
EP1212430A1 (en) Nuclease variant and their use in genotyping assays
JP3702182B2 (ja) ピロバクルム・イスランジカム（Ｐｙｒｏｂａｃｕｌｕｍｉｓｌａｎｄｉｃｕｍ）由来のＤＮＡポリメラーゼ
WO2006081463A2 (en) Thermococcus zilligii dna polymerases and variants thereof
JP2009508488A (ja) サーモコッカス種（９°ｎ−７株）ｄｎａリガーゼの発見、クローニング及び精製
Susanti et al. Cloning, homological analysis, and expression of DNA Pol I from Geobacillus thermoleovorans
RU2405823C2 (ru) Термостабильная днк-лигаза из археи рода thermococcus, способ ее получения и нуклеотидная последовательность днк, кодирующая эту днк-лигазу
JPH07163343A (ja) 耐熱性ｄｎａポリメラーゼ
Class et al. Patent application title: METHODS FOR AMPLIFICATION AND SEQUENCING USING THERMOSTABLE TTHPRIMPOL Inventors: Angel J. Picher (Madrid, ES) Luis Blanco (Madrid, ES) Assignees: SYGNIS BIOTECH SLU
IL101060A (en) Composition comprising modified T7 dna polymerase and preparation thereof

Legal Events

Date Code Title Description
RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20041124

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20041209

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20041209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20060613

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20060613

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20081216

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20090316

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20090324

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090415

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20090415

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20090415

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20090519

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20090611

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20090716

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120731

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120731

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120731

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130731

Year of fee payment: 4

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees