JP4001633B2 - プレフォームドポリマーのコーティング方法及び製品 - Google Patents

プレフォームドポリマーのコーティング方法及び製品 Download PDF

Info

Publication number
JP4001633B2
JP4001633B2 JP52774898A JP52774898A JP4001633B2 JP 4001633 B2 JP4001633 B2 JP 4001633B2 JP 52774898 A JP52774898 A JP 52774898A JP 52774898 A JP52774898 A JP 52774898A JP 4001633 B2 JP4001633 B2 JP 4001633B2
Authority
JP
Japan
Prior art keywords
polymer
carbon
coating
coupling agent
preformed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP52774898A
Other languages
English (en)
Japanese (ja)
Other versions
JP2001506364A5 (enExample
JP2001506364A (ja
Inventor
カーナン スピーニヴァサン
ネボイサ アヴダロヴィック
クリストファー エイ ポール
Original Assignee
ダイオネックス コーポレイション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ダイオネックス コーポレイション filed Critical ダイオネックス コーポレイション
Publication of JP2001506364A publication Critical patent/JP2001506364A/ja
Publication of JP2001506364A5 publication Critical patent/JP2001506364A5/ja
Application granted granted Critical
Publication of JP4001633B2 publication Critical patent/JP4001633B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30Processes for preparing, regenerating, or reactivating
    • B01J20/32Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204Inorganic carriers, supports or substrates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30Processes for preparing, regenerating, or reactivating
    • B01J20/32Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206Organic carriers, supports or substrates
    • B01J20/3208Polymeric carriers, supports or substrates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30Processes for preparing, regenerating, or reactivating
    • B01J20/32Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30Processes for preparing, regenerating, or reactivating
    • B01J20/32Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268Macromolecular compounds
    • B01J20/3278Polymers being grafted on the carrier
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30Processes for preparing, regenerating, or reactivating
    • B01J20/32Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268Macromolecular compounds
    • B01J20/328Polymers on the carrier being further modified
    • B01J20/3282Crosslinked polymers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44752Controlling the zeta potential, e.g. by wall coatings

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Graft Or Block Polymers (AREA)
  • Processing And Handling Of Plastics And Other Materials For Molding In General (AREA)
JP52774898A 1996-12-19 1997-12-10 プレフォームドポリマーのコーティング方法及び製品 Expired - Lifetime JP4001633B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/770,628 US5792331A (en) 1996-12-19 1996-12-19 Preformed polymer coating process and product
US08/770,628 1996-12-19
PCT/US1997/022033 WO1998027418A1 (en) 1996-12-19 1997-12-10 Preformed polymer coating process and product

Publications (3)

Publication Number Publication Date
JP2001506364A JP2001506364A (ja) 2001-05-15
JP2001506364A5 JP2001506364A5 (enExample) 2005-08-11
JP4001633B2 true JP4001633B2 (ja) 2007-10-31

Family

ID=25089207

Family Applications (1)

Application Number Title Priority Date Filing Date
JP52774898A Expired - Lifetime JP4001633B2 (ja) 1996-12-19 1997-12-10 プレフォームドポリマーのコーティング方法及び製品

Country Status (6)

Country Link
US (2) US5792331A (enExample)
EP (1) EP0948742A4 (enExample)
JP (1) JP4001633B2 (enExample)
AU (1) AU724288B2 (enExample)
CA (1) CA2274536C (enExample)
WO (1) WO1998027418A1 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537432B1 (en) 1998-02-24 2003-03-25 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
KR20010041535A (ko) 1998-03-04 2001-05-25 아리조나 보드 오브 리전츠 인가된 외부 전압장에 의하여 전기삼투를 제어하는 화학적처리된 표면
US6099852A (en) * 1998-09-23 2000-08-08 Johnson & Johnson Vision Products, Inc. Wettable silicone-based lenses
US6402918B1 (en) * 1998-11-16 2002-06-11 Joseph B. Schlenoff Apparatus for capillary electrophoresis and associated method
EP1155315A1 (de) * 1999-02-22 2001-11-21 Evotec BioSystems AG Verwendung von trägermaterial in der kapillar-elektrochromatographie
DE50002587D1 (de) 1999-03-09 2003-07-24 Ganomycin Ges Fuer Biomedizini Biologisch aktive verbindungen aus ganoderma pfeifferi dms 13239
US6764817B1 (en) 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
US6818112B2 (en) * 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6375818B1 (en) 1999-06-08 2002-04-23 Beckman Coulter, Inc. Surfaces with reduced electroosmotic flow
DE19938002A1 (de) * 1999-08-11 2001-02-15 Studiengesellschaft Kohle Mbh Beschichtung mit quervernetzten hydrophilen Polymeren
US6689715B1 (en) * 2000-02-09 2004-02-10 Hammen Corporation Tethered polymer ligands
US6787016B2 (en) * 2000-05-01 2004-09-07 Aclara Biosciences, Inc. Dynamic coating with linear polymer mixture for electrophoresis
US7106818B2 (en) * 2000-10-17 2006-09-12 Koninkijke Philips Electronics N.V. Method and apparatus for timing recovery based on dispersion characterization and components therefor
US6825032B2 (en) * 2001-05-14 2004-11-30 Sigma-Aldrich Co. High capacity assay platforms
US6867295B2 (en) * 2001-09-07 2005-03-15 Dionex Corporation Ion exchange cryptands covalently bound to substrates
US6994791B2 (en) 2002-06-27 2006-02-07 Akzo Nobel N.V. Adsorbent material and method of preparing an adsorbent material
US6923895B2 (en) * 2002-09-09 2005-08-02 Beckman Coulter, Inc. Coated capillary electrophoresis tubes and system
US7303821B1 (en) 2003-07-24 2007-12-04 Sepax Technologies, Inc. Material and process for precisely controlled polymeric coatings
US7147891B2 (en) * 2004-02-11 2006-12-12 Dionex Corporation Polymer substrate coated with weak cationic-exchange functional layer
US7291395B2 (en) * 2004-02-18 2007-11-06 Dionex Corporation Coated ion exchanged substrate and method of forming
ATE547442T1 (de) * 2004-04-02 2012-03-15 Nexttec Gmbh Verfahren zur herstellung eines verbundsorptionsmaterials für die chromatographische trennung von biopolymeren
US20090252965A1 (en) * 2005-04-29 2009-10-08 Eidgenössische Technische Hochschule Zürich Surface functionalization and coating of flame-generated nanoparticles
US20070014699A1 (en) 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
US9283494B2 (en) 2005-09-16 2016-03-15 Dionex Corporation Agglomerated ion exchange particle bed and method
JP2009511917A (ja) * 2005-10-14 2009-03-19 アドビオン バイオシステムズ インコーポレイテッド 液体と電極との接触を伴う分析装置における分析物の変質を防ぐ方法
JP4129054B2 (ja) * 2006-10-16 2008-07-30 積水化学工業株式会社 ヘモグロビン類の測定方法
JP4912867B2 (ja) * 2006-12-28 2012-04-11 積水化学工業株式会社 ヘモグロビン類の測定方法
JP4854502B2 (ja) * 2006-12-26 2012-01-18 積水化学工業株式会社 ヘモグロビン類の測定方法
EP2623975B1 (en) 2006-12-26 2016-02-03 Sekisui Chemical Co., Ltd. Stable hemoglobin A1c and glucose measurement method
US20080188010A1 (en) * 2007-02-01 2008-08-07 Fujifilm Corporation Biosensor substrate
TW201041131A (en) * 2009-03-09 2010-11-16 Sekisui Chemical Co Ltd Underside protective sheet for solar cell, solar cell module, and gas-barrier film
US8470074B2 (en) * 2009-12-22 2013-06-25 Exxonmobil Research And Engineering Company Carbon dioxide sorbents
US9132364B2 (en) 2010-02-26 2015-09-15 Dionex Corporation High capacity ion chromatography stationary phases and method of forming
JP5474881B2 (ja) * 2010-07-28 2014-04-16 ローム アンド ハース カンパニー 向上したクロマトグラフィー媒体の製造方法および使用方法
CN103492077B (zh) 2011-03-11 2015-11-25 迪奥内克斯公司 使用叔胺连接基经由缩聚反应制备的、静电键合的、超支化的、用于二价阴离子选择性改进的阴离子交换表面涂层
WO2014085486A2 (en) 2012-11-30 2014-06-05 Waters Technologies Corporation Methods and apparatus for the analysis of vitamin d metabolites
WO2017022695A1 (ja) * 2015-07-31 2017-02-09 株式会社ダイセル 超臨界流体クロマトグラフィー用の固定相
WO2017164289A1 (ja) * 2016-03-23 2017-09-28 株式会社ダイセル クロマトグラフィー用の固定相
WO2018200279A1 (en) 2017-04-24 2018-11-01 University Of Notre Dame Du Lac Tunable electroosmotic flow polymer coated capillary

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE328715B (enExample) * 1967-04-20 1970-09-21 Incentive Res & Dev Ab
JPS5146552B2 (enExample) * 1972-12-04 1976-12-09
US4137367A (en) * 1975-12-03 1979-01-30 Dresser Industries, Inc. Silane coated silicate minerals and method for preparing same
JPS608688B2 (ja) * 1979-06-13 1985-03-05 日東化学工業株式会社 アクリルアミド系重合体の製造方法
US4454234A (en) * 1981-12-30 1984-06-12 Czerlinski George H Coated magnetizable microparticles, reversible suspensions thereof, and processes relating thereto
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4495245A (en) * 1983-07-14 1985-01-22 E. I. Du Pont De Nemours And Company Inorganic fillers modified with vinyl alcohol polymer and cationic melamine-formaldehyde resin
US4680201A (en) * 1985-10-30 1987-07-14 Stellan Hjerten Coating for electrophoresis tube
US4690749A (en) * 1985-12-16 1987-09-01 Universities Space Research Association Polymer-coated surfaces to control surface zeta potential
US5089106A (en) * 1986-10-21 1992-02-18 Northeastern University High performance capillary gel electrophoresis
US4865706A (en) * 1986-10-21 1989-09-12 Northeastern University High performance microcapillary gel electrophoresis
US4997537A (en) * 1986-10-21 1991-03-05 Northeastern University High performance microcapillary gel electrophoresis
US5205929A (en) * 1988-02-03 1993-04-27 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US4931328A (en) * 1988-08-19 1990-06-05 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow
US5017540A (en) * 1989-09-15 1991-05-21 Sandoval Junior E Silicon hydride surface intermediates for chemical separations apparatus
US5164055A (en) * 1990-01-29 1992-11-17 Applied Biosystems, Inc. High-viscosity polymer matrix and methods
DE69007773T2 (de) * 1990-02-13 1994-09-22 Bio Rad Laboratories Elektrophoretische Trennung in einem gelfreien Medium mittels gelösten Polymeren.
US5074982A (en) * 1990-10-26 1991-12-24 Indiana University Foundation Suppression of electroosmosis with hydrolytically stable coatings
US5221447A (en) * 1991-12-06 1993-06-22 Bio-Rad Laboratories, Inc. Hydrophilic polymer coating of high pH stability for silica surfaces for suppression of electroendomosis and solute adsorption
US5322608A (en) * 1992-12-23 1994-06-21 Northeastern University Siloxandediol coating for capillary electrophoresis and for general surface modification
US5447617A (en) * 1994-01-25 1995-09-05 Beckman Instruments, Inc. Coated capillary columns and electrophoretic separation methods for their use
US5605613A (en) * 1994-01-25 1997-02-25 Beckman Instruments, Inc. Polyvinylalcohol coated capillary electrophoresis columns

Also Published As

Publication number Publication date
US6074541A (en) 2000-06-13
EP0948742A4 (en) 2006-04-12
JP2001506364A (ja) 2001-05-15
AU7739498A (en) 1998-07-15
WO1998027418A1 (en) 1998-06-25
US5792331A (en) 1998-08-11
CA2274536C (en) 2006-06-06
EP0948742A1 (en) 1999-10-13
AU724288B2 (en) 2000-09-14
CA2274536A1 (en) 1998-06-25

Similar Documents

Publication Publication Date Title
JP4001633B2 (ja) プレフォームドポリマーのコーティング方法及び製品
US5135627A (en) Mosaic microcolumns, slabs, and separation media for electrophoresis and chromatography
US6372353B2 (en) Coated surface comprising a polyvinyl alcohol (PVA) based covalently bonded stable hydrophilic coating
EP0172579B1 (en) Modified siliceous supports
US5503933A (en) Covalently bonded coatings
JP4440771B2 (ja) 表面改質ベースマトリックス
US5447617A (en) Coated capillary columns and electrophoretic separation methods for their use
US5074982A (en) Suppression of electroosmosis with hydrolytically stable coatings
CN108883394B (zh) 色谱基质
Huang et al. Capillary zone electrophoresis with fluid-impervious polymer tubing inside a fused-silica capillary
Huang et al. High efficiency cross‐linked polyacrylamide coating for capillary electrophoresis of proteins
CN112292203B (zh) 色谱珠、其生产及用途
US5605613A (en) Polyvinylalcohol coated capillary electrophoresis columns
CN104628958A (zh) 聚苯乙烯类或其共聚物材料的亲水改性方法及材料
US5143753A (en) Suppression of electroosmosis with hydrolytically stable coatings
US5292818A (en) Method for producing a carrier for cation exchange liquid chromatography and a method for determining glycosylated hemoglobins using the carrier
JPH05249080A (ja) 高速キャピラリーゲル電気泳動方法、およびそのカラムとその調整方法
Kuban et al. Ni (II) functionalized polyhedral oligomeric silsesquioxane based capillary monolith for purification of histidine-tagged proteins by immobilized metal affinity micro-chromatography
JP3424255B2 (ja) 液体クロマトグラフィー用充填剤の製造方法
JP2559525B2 (ja) 液体クロマトグラフィー用充填剤の製造方法
JPH0219902B2 (enExample)
Currivan et al. Modification reactions applicable to polymeric monolithic columns. A review
Ivanov et al. Structures of grafted polymer phases and features of protein separation on composite anion-exchangers
JP2559525C (enExample)
WO1992009889A1 (en) Process for preparing carrier for cation exchange liquid chromatography and method of quantitatively determining saccharified hemoglobin using said carrier

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20041206

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20041206

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20061205

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20070223

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20070409

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20070605

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20070724

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20070815

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20100824

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110824

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20110824

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120824

Year of fee payment: 5

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130824

Year of fee payment: 6

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term