JP3868345B2 - 皮膚状態の鑑別法 - Google Patents
皮膚状態の鑑別法 Download PDFInfo
- Publication number
- JP3868345B2 JP3868345B2 JP2002213266A JP2002213266A JP3868345B2 JP 3868345 B2 JP3868345 B2 JP 3868345B2 JP 2002213266 A JP2002213266 A JP 2002213266A JP 2002213266 A JP2002213266 A JP 2002213266A JP 3868345 B2 JP3868345 B2 JP 3868345B2
- Authority
- JP
- Japan
- Prior art keywords
- keratinocytes
- aqueous carrier
- keratinocyte
- water
- collection tool
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000012850 discrimination method Methods 0.000 title 1
- 210000002510 keratinocyte Anatomy 0.000 claims description 63
- 239000008365 aqueous carrier Substances 0.000 claims description 27
- 239000003795 chemical substances by application Substances 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 19
- 239000000853 adhesive Substances 0.000 claims description 15
- 210000000736 corneocyte Anatomy 0.000 claims description 15
- 230000001070 adhesive effect Effects 0.000 claims description 14
- 239000004094 surface-active agent Substances 0.000 claims description 12
- 239000011248 coating agent Substances 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 11
- 239000006185 dispersion Substances 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 229920001577 copolymer Polymers 0.000 claims description 7
- 238000009736 wetting Methods 0.000 claims description 5
- 230000008961 swelling Effects 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 25
- 210000003491 skin Anatomy 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical group P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 229920004890 Triton X-100 Polymers 0.000 description 5
- 239000013504 Triton X-100 Substances 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 239000002390 adhesive tape Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 229920002689 polyvinyl acetate Polymers 0.000 description 4
- 239000011118 polyvinyl acetate Substances 0.000 description 4
- 206010012438 Dermatitis atopic Diseases 0.000 description 3
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 201000008937 atopic dermatitis Diseases 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 210000001047 desmosome Anatomy 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- -1 polyethylene terephthalate Polymers 0.000 description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 description 3
- 239000005020 polyethylene terephthalate Substances 0.000 description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 3
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 239000008096 xylene Substances 0.000 description 3
- JPPRXACMNPYJNK-UHFFFAOYSA-N 1-docosoxydocosane Chemical compound CCCCCCCCCCCCCCCCCCCCCCOCCCCCCCCCCCCCCCCCCCCCC JPPRXACMNPYJNK-UHFFFAOYSA-N 0.000 description 2
- 102000003908 Cathepsin D Human genes 0.000 description 2
- 108090000258 Cathepsin D Proteins 0.000 description 2
- 108090000317 Chymotrypsin Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 229960002376 chymotrypsin Drugs 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000001863 hydroxypropyl cellulose Substances 0.000 description 2
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 2
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 2
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 2
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 2
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000009210 therapy by ultrasound Methods 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- IMROMDMJAWUWLK-UHFFFAOYSA-N Ethenol Chemical compound OC=C IMROMDMJAWUWLK-UHFFFAOYSA-N 0.000 description 1
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229920002845 Poly(methacrylic acid) Polymers 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 description 1
- QYKIQEUNHZKYBP-UHFFFAOYSA-N Vinyl ether Chemical compound C=COC=C QYKIQEUNHZKYBP-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000000337 buffer salt Substances 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004299 exfoliation Methods 0.000 description 1
- 239000007951 isotonicity adjuster Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920001289 polyvinyl ether Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002784 sclerotic effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002213266A JP3868345B2 (ja) | 2002-07-23 | 2002-07-23 | 皮膚状態の鑑別法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002213266A JP3868345B2 (ja) | 2002-07-23 | 2002-07-23 | 皮膚状態の鑑別法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006200495A Division JP4395152B2 (ja) | 2006-07-24 | 2006-07-24 | 皮膚状態の鑑別法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004053491A JP2004053491A (ja) | 2004-02-19 |
| JP2004053491A5 JP2004053491A5 (enrdf_load_stackoverflow) | 2005-04-07 |
| JP3868345B2 true JP3868345B2 (ja) | 2007-01-17 |
Family
ID=31935906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002213266A Expired - Fee Related JP3868345B2 (ja) | 2002-07-23 | 2002-07-23 | 皮膚状態の鑑別法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3868345B2 (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018043636A1 (ja) | 2016-08-31 | 2018-03-08 | 株式会社ヤクルト本社 | 皮膚分析又は観察用検体の調製方法 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6974980B2 (ja) * | 2017-07-06 | 2021-12-01 | 泰子 別所 | 細胞処理試薬及び顕微鏡標本を作製する方法 |
| CN112105908A (zh) * | 2018-05-17 | 2020-12-18 | 株式会社资生堂 | 角质层采集用粘合组合物、角质层采集工具、生物物质提取试剂盒和生物物质采集方法 |
-
2002
- 2002-07-23 JP JP2002213266A patent/JP3868345B2/ja not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018043636A1 (ja) | 2016-08-31 | 2018-03-08 | 株式会社ヤクルト本社 | 皮膚分析又は観察用検体の調製方法 |
| KR20190046784A (ko) | 2016-08-31 | 2019-05-07 | 가부시키가이샤 야쿠르트 혼샤 | 피부 분석 또는 관찰용 검체의 조제 방법 |
| US11644458B2 (en) | 2016-08-31 | 2023-05-09 | Kabushiki Kaisha Yakult Honsha | Method for preparing specimen for analysis or observation of skin |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004053491A (ja) | 2004-02-19 |
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| Marks | Seeing through the stratum corneum |
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