JP3507347B2 - Aqueous liquid containing humus extract material - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an aqueous liquid containing a humus soil extraction substance. More specifically, antibacterial and bactericidal action,
The present invention relates to a humus-extract-containing substance-containing aqueous liquid having a virus inactivating action, an antioxidant action, an anticorrosive action, a body purifying action, a lipolytic action, an alcohol degrading action, a surfactant action, a cholesterol degrading action and the like.

[0002]

2. Description of the Related Art Generally, humus soils are seagrass, plants, algae, seafood, and other minerals deposited on the seabed, lakes, swamps, etc. over 5 million years ago due to anaerobic microorganisms. It has been decomposed, synthesized, and organized, and exists as a layer of about 10 m in about 20 m underground.

As a method using an extract from humus soil, Japanese Patent Publication No. Sho 62-3806 discloses that the humic acid solution in water obtained by extracting humic acid in humus soil with water is sterilized by heating to 0.6 μm. It is described that an aqueous humic acid solution, which has been filtered with a filter of No. 3, adjusted in concentration and adjusted to pH 2.0 to 5.0, is used as a bactericide for food preservation.

[0004]

In the production of the humic acid aqueous solution described in the above publication, heat sterilization is performed, so that the characteristics of the humic acid aqueous solution change. In other words, humus is composed of many components such as amino acids, vitamins, proteins, enzymes and minerals, but it is easy to conjecture that heating changes the amino acids and proteins and the humic substances. . Therefore, in the case of the humic acid aqueous solution, there is a problem to what extent the desirable properties of the original humus are retained.

Therefore, the humic acid aqueous solution which has been subjected to the heat treatment has a bactericidal action due to the mineral component and the pH value of 2.0 to 5.0 which are not significantly changed by the heat treatment. Effects of amino acids, proteins, vitamins, etc. (for example,
It cannot be expected to have the effect of activating the metabolism of the skin), and does not have an excellent anticorrosion effect, a dissolution effect of heavy metals and harmful chemical substances, and a cholesterol decomposition effect.

In addition, even though the heat treatment is performed, 2
Storage stability is not good, such as browning after storage for ~ 3 months.

[0007]

DISCLOSURE OF THE INVENTION The present invention is an aqueous liquid containing a humus-extracting substance, which retains the desirable properties inherent in humus (for example, various useful actions such as antibacterial and bactericidal actions) and which is simple in method. The humus soil in the form of a block having a diameter of about 5 to 50 cm was collected.
Water is added to the humus obtained by a two-step drying process: rough drying in which it is dried in the sun for 6 months to 1 year, and precision drying in a greenhouse for 1 to 2 months. In addition, extraction was performed, and the resulting extract was added to 0.1
Obtained by filtration through a filter with ～0.2Myuemu, humus extractant containing aqueous solution a pH of the filtrate which is not heat sterilization of from 2.50 to 2.99 (claim 1), and extracted The ratio of humus to water is 1: 3
To 1: about the 5 humus extracted substance-containing aqueous solution of claim 1 wherein the (weight / volume) (claim 2).

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION The humus used in the present invention may be obtained from any of the humus soils, but humus soils collected from Karahinai, Moriyama Town, Kitatakagi District, Nagasaki Prefecture are particularly preferred.

The collected humus soil contains water and is sticky like soil taken from paddy fields, and is subjected to a two-stage drying process of rough drying and precision drying before being subjected to the extraction process. It In the drying process, the humus soil is dried and subdivided, the functions of aerobic microorganisms are activated to suppress the functions of anaerobic bacteria, and solar heat sterilization is carried out, while at the same time absorption of ultraviolet rays (photosynthesis) causes amino acids, It is thought to activate (age) vitamins and enzymes. This is considered to have beneficial effects such as an antioxidant effect and an anticorrosion effect. By performing the drying process in two stages, it is possible to make the constituents uniform, remove mud other than humus, and obtain a stable corrosive soil-extracting substance-containing aqueous liquid with less variation in pH during extraction. In the rough drying, first, lumped humus with a diameter of about 5 to 50 cm that is not mixed with humus such as concrete is rain-dried, and then dried for half a year to one year in a sunlit state. Performed by. During this period, it is preferable that the humus is appropriately turned over so that the whole is exposed to the sun. This process turns the humus into a dry mass. Then, it is further dried in a greenhouse (precision drying). This drying takes at least 1 month, usually 1.
It will be held for 5 to 2 months. As a result, the lumps are further dried, and the lumps are further pulverized with a cultivator or the like to give a sandy, smooth lump. The term "humus soil" as used in the present invention means dried obtained through the above-mentioned steps,
It is ground humus. Such humus is commercially available, for example, in the form of a bag packaged under the trade name of FCM powder from Karako Sangyo Co., Ltd.

Table 1 shows an example of the properties of the obtained humus soil.

[0011]

[Table 1]

The humus-extract-containing substance-containing aqueous liquid of the present invention is obtained by extracting the humus soil with water. The water used for the extraction may be any water, for example groundwater, well water,
Tap water, purified water, distilled water or the like can be used. Above all, groundwater and well water containing minerals are preferable because water containing a large amount of mineral components is preferable to obtain an extract having a low pH and no chlorine-based disinfectant is contained.

The extraction can be performed by stirring the humus and water. The temperature at the time of extraction is usually room temperature. The stirring time is usually 1 to 3 hours, preferably 2 to 3 hours.

The preferred ratio of humus to water used is:
1: 1 to 1: 5, further 1: 3 to 1: 5, especially 1: 5 (weight / volume ratio). When the amount of water is less than the above range, the yield of the extract is small with respect to the humus used, and even when the amount is decreased, the obtained pH does not become lower than a certain value, which is meaningless. on the other hand,
If the amount of water is more than the above range, the pH of the extract obtained will be higher than 2.99, and depending on the type of bacteria or virus, the sterilization, sterilization, antiviral effect, etc. will not be sufficient and the use will be limited. There are cases. However, even when the obtained extract has a high pH, or when the extract having a low pH is diluted to a liquid having a high pH, it can be used for applications where a high pH is sufficient. In this case as well,
The aqueous humic acid solution described in Japanese Patent No. 3806 can be used for a wider range of applications than those having the same pH.

2 to 4 weeks after the completion of stirring, preferably about 3
After allowing to stand for a week to settle the suspended fine particles, the supernatant is separated by a separation method usually used in a filtration step such as decantation, suction, and preliminary filtration with a bag filter of 50 to 100 μm. When the suction port is located 5 to 10 cm above the interface between the supernatant and the humus layer and suction is performed, the supernatant can be sucked in a high yield without sucking the humus layer. .

Next, the extract is filtered using a filter of 0.3 μm or less. By carrying out this filtration, it is possible to remove various bacteria, eliminate suspended matter, suspended matter, or insoluble substances that precipitate over time, and obtain clear fresh water suitable for beverages. An extract as described above cannot be obtained with a filter having a pore size larger than 0.3 μm.

The pore size of the filter used is 0.3 μm or less, preferably 0.2 μm or less (in the present invention, 0.2 μm).
And below) , and the lower limit is 0.1 μm. The filter material of the filter is not limited as long as it does not affect the filtrate,
Specific examples include nylon, polypropylene, polyvinylidene fluoride and the like. Among these, polyvinylidene fluoride is preferable.

The filtration may be carried out under pressure or under reduced pressure, but generally it is carried out under pressure. The method is carried out, for example, by setting a cartridge of a membrane filter of the above material in a housing, connecting this to a pressure tank, and pressure-feeding at a pressure of ² kg / cm ² or less.
It is preferable to carry out preliminary filtration before performing this filtration from the viewpoint of improving the efficiency of filtration and reducing the burden on the filtration device. In practice, it is sufficient to filter with a bag filter of 50 to 100 μm.

The resulting filtrate preferably has a pH of 2.50.
˜2.99, more preferably pH 2.85 to 2.95, especially pH 2.9. If the pH is less than 2.50, it is not usually obtained. If it exceeds 2.99, the bactericidal or sterilizing antiviral effect may not be sufficient depending on the type of bacteria or virus, resulting in the anticorrosive effect. And the antioxidant power will decrease. It is difficult to obtain a filtrate having a pH lower than the above range by a means other than modifying the extract such as adding an acid (for example, hydrochloric acid) or heating. For example, even if the pH is lowered by adding an acid (for example, hydrochloric acid) to the one having a high pH, the performance is inferior when compared with the one having the same pH without doing anything.

The humus-extract-extracted substance-containing aqueous liquid of the present invention obtained as described above contains humic acid, fulvic acid, humin and other humus-constituting substances, lipase and other lipolytic enzymes, and alcohol. Enzymes such as dehydrogenase and other alcohol degrading enzymes, proteins, amino acids, fatty acids, organic acids, vitamins, minerals, reducing substances, etc. are contained.

Humic acid, also called humic acid, is a component forming most of organic matter and coal quality in soil.
Although the chemical structure of humic acid is unknown, it is a condensation product of a polyhydric phenol type aromatic compound and a nitrogen-containing compound, and has a phenolic hydroxyl group and a carboxyl group. However, since the molecular weight and constituents of humic acid vary greatly depending on the degree of progress of humus, a decisive judgment cannot be made.
It is considered that 0 to 55% is composed of amino acids, hexosamine, polycyclic aromatic compounds, and compounds having an oxygen-containing functional group, and the rest are compounds having unknown chemical structures and many double bonds.

Since the humus extract-containing aqueous liquid of the present invention contains the above-mentioned substances, it has antibacterial / bactericidal action, virus inactivating action, antioxidant action, anticorrosive action, internal purifying action, and lipolysis. In addition to its action, alcohol decomposing action, surface active action, cholesterol degrading action, etc., it has active oxygen deactivating action, heavy metal dissolving action, and harmful chemical substance dissolving / neutralizing ability.

These effects are obtained by enzymes synthesized by microorganisms, enzymes obtained by decomposition of dead microorganisms, amino acids synthesized by microorganisms from the remains of plants of the Poaceae family that first form humus, protein,
It is obtained by mutual interaction between vitamins and enzymes, and minerals such as magnesium, calcium, sodium, potassium, and aluminum deposited by invasion of igneous rock. Among other things, aluminum as a mineral,
It contains a large amount of ions such as magnesium, sodium, and calcium.
Since the value increases, it is considered that the sterilization mechanism of the aqueous solution containing the humus extract material of the present invention largely affects the low pH and minerals exhibited by humic acid.

The humus-extract-containing substance-containing aqueous liquid of the present invention may be used as it is, or may be used after being diluted appropriately according to the intended use. If you want to drink
You can drink it as it is without diluting it, or you can dilute it by 2 to 500 times.

When the aqueous liquid containing the humus-extracting substance of the present invention is to be drunk, it is preferable to pass it through a cation exchange resin to remove aluminum ions. At this time, other cations are also removed.

The humus-extract-extracting substance-containing aqueous liquid of the present invention can be used as it is, for example, in the following usage.

When used as an antibacterial agent / bactericidal agent, for example, a handwash soap tank containing the aqueous solution containing the humus-extracting substance of the present invention is provided in a handwashing room at the entrance of a dining room, etc. Can be done. In addition, sterilization can be carried out by putting the humus-extract-extract-containing aqueous solution of the present invention in a spray-type container and spraying it on dishes, cooking utensils, fingers and the like. Washing and sterilizing fresh foods before cooking is effective for food poisoning prevention and hygiene, and improves the freshness of foods. Also, gargling with an appropriately diluted aqueous solution is effective against stomatitis, alveolar pyorrhea, influenza, colds and the like. If used in cosmetics, acne, atopic skin,
Effective for rough skin. Examples of the target bacteria include Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Legionella, Salmonella, Vibrio parahaemolyticus, and various other Gram-positive and Gram-negative bacteria and fungi.

When used as a virus inactivating agent, for example, against herpes virus and influenza virus, a stock solution to a 2-fold dilution may be used to prevent infection by gargle, and AIDS virus may be drunk or washed locally. By doing, it will lead to prevention and prevention of infection.

When it is used as an antioxidant, for example, when it is added to foods, drinking water, and drinks for the purpose of preventing oxidation, preventing spoilage, the product life can be expected to be extended safely. Further, when used in the field of cosmetics, it is possible to prevent oxidative deterioration of the compounding base (especially oily base).
For other industrial purposes, it can be used as cleaning water for lubricating rust-prone areas and as lubricating water.

When it is used as an anticorrosion agent, when it is added to foods, drinking water, and drinks, an anticorrosion effect as well as an antioxidant effect is obtained. Low p for bacteria, mold and bacteria
Since they cannot survive in the H region, their occurrence can be stopped. When used in cosmetics, it acts as a natural anticorrosion agent and can be used in place of parabens and others.

When used as a lipolytic agent, it contains a lipolytic enzyme represented by lipase, so that it can reduce body fat and promote combustion decomposition of excessively ingested fat such as fatty liver and hyperlipidemia. It is thought to be possible. Especially, it is desirable to drink after removing too much oil.

When used as an alcohol degrading agent, it contains an alcohol degrading enzyme such as alcohol dehydrogenase. Therefore, if it is drunk at a 10-fold dilution after drinking, the ingested alcohol can be quickly decomposed and hangover can be prevented. It is considered possible. It is also possible that the weakened liver function can be normalized.

When used as a surfactant, the components contained in the humus-extract-extracted substance-containing aqueous liquid of the present invention are only naturally occurring substances, so that the problem is that they have an environmental hormone-like action. It is a substitute for activator. When used in cosmetics, it can be used as a substitute for reducing the amount of cationic surfactants, anionic surfactants, and nonionic surfactants currently used in the production of emulsions and creams. Furthermore, the stains that are created by the addition of soy sauce,
For example, the undiluted solution can be used as a stain remover.

When used as a cholesterol-degrading agent,
The aqueous solution containing the humus extract material of the present invention contains fatty acid, organic acid,
Since it contains enzymes such as lipase, it is thought that if it is appropriately diluted and then drunk, it can directly act on the liver to enhance its function and burn and decompose cholesterol. If blood cholesterol etc. are decomposed,
It can be used for blood coagulation prevention because the viscosity of blood is lowered and the blood flow is improved.

Urease enzyme activity is associated with neuralgia, rheumatism,
Since it promotes the decrease of uric acid level found in gout and the like, it has a common pain-relieving effect, and mixing about 10 cc of water with 100 cc of water leads to relief of pain and the like.

When used as an active oxygen deactivator, the reducing substance contained in the humus extract-containing aqueous solution of the present invention deactivates the active oxygen that causes aging of the human body, and is therefore appropriately diluted. By drinking it, oxidation in the body, so-called aging, can be prevented.

Further, the aqueous liquid containing humus-extracting substance of the present invention can be used in various other industries listed below.

When used in the cosmetics industry, for example, cosmetics having a moisturizing effect, cosmetics having an action of suppressing acne, pimples and inflammations, cosmetics for the purpose of activating metabolism, cosmetics having excellent antioxidant power, Cosmetics that use themselves as natural surfactants and / or natural anticorrosives, cosmetics that are additive-free, fragrance-free, and non-colored, cosmetics whose cosmetic ingredients are detoxified or alleviated, cosmetics that do not cause skin irritation, and secretions Cosmetics or etiquette products having a deodorant effect for the purpose of regulating the effect of shampoo and conditioner having anti-aging effect on the scalp,
It is possible to manufacture treatments, hair thickeners or hair restorers, natural mineral-containing cosmetics, low-pH weakly acidic cosmetics, and the like.

When used in the food industry, sterilization of fresh food, sterilization and freshness maintenance, cleaning of food equipment,
Sterilization and sterilization, antioxidation of general foods, addition of antioxidant foods, detoxification and mitigation of food additives, extension of food storage period, prevention of deterioration and deterioration of foods, stabilization of effects of microbial products It has the effect of improving the taste, sterilizing and sterilizing in the manufacturing plant, and improving the taste by adding it to the product.

When used in the medical field, prevention of nosocomial infection, cleaning and disinfection of equipment, cleaning of the oral cavity, reduction of halitosis accompanying it, prevention of bed sore of inpatients and hygiene management of bedridden patients, disinfection and sterilization of fingers It is effective in cleaning bladder, cleaning anus, cleaning an operating room and a sterile room, and sterilizing clothes, hats and shoes of medical workers.

When used in agriculture, disinfection and sterilization in organic farming, germination of crops, promotion of rooting and development, improvement of soil, enhancement of resistance to pests and fungi, improvement of productivity, and reduction of fertilizer cost. It has effects such as reduction, cultivation of nutritious crops, decomposition and neutralization of harmful substances contained in chemical fertilizers and pesticides.

When used in the fishery and aquaculture industries, the effects of maintaining and improving freshness of marine products, improving meat quality, improving disease resistance, promoting growth, improving color and luster, supplementing micronutrients and the like are exhibited. It In the livestock industry, there are improvements in meat quality, disease resistance, and reduction of manure odor.

In addition, as a hand-washing water, gargle water, sterilizing / sterilizing water in a kitchen, or indoor sterilizing, sterilizing and purifying water for prevention of infection in places where many people come and go such as schools, kindergartens and nurseries. It can be used for the purpose of water quality retention, anticorrosion and sterilization of water tanks, industrial waste treatment, sludge, dioxin and PCB decomposition.

As described above, the humus-extract-containing aqueous solution of the present invention can be diluted and used in the food industry, medical industry, cosmetic industry, agricultural industry field or households. For example, when diluted 10-fold, the pH will be 3.65, but since it is effective against most bacteria other than viruses and bacteria that cause food poisoning, it can be used as a stock solution (eg pH 2 when used for other than viruses). The effect is almost the same as in (9). When diluted 100 times,
Although the pH is 4.35, in this case, the sterilization effect varies and the effective range is narrowed. However, since Staphylococcus aureus and the like can be sterilized by 100%, it is desirable to dilute the bacterium according to the purpose of use. Further, when diluted 500 times, the pH becomes 4.68. In this case, it is preferable to use it for the purpose of sterilization and disinfection rather than the sterilization effect. In household use and in the cosmetics industry, this dilution ratio has a wide range of applications. In addition, for example, if a diluted product of 10 to 50 times is used for cleaning the baby's buttocks, it is possible to prevent rashes and disinfect the buttocks.

[0045]

EXAMPLES The humus soil extract-containing aqueous liquid of the present invention is described in more detail below with reference to Examples, but the present invention is not limited thereto.

Example 1 Humus was collected from the marshland of Karahinishi, Moriyama-cho, Kitakorai-gun, Nagasaki Prefecture. The collected humus soil was exposed to the sun on the concrete for about one year to dry. Then, it was further dried in a vinyl house for 1.5 months. The obtained humus soil was crushed into particles having a particle size of about 0.01 to 0.5 mm.

About 140 kg of the obtained humus soil was placed in a 1 t tank (made of polyethylene) (the capacity when placed in a 1 t tank was about 0.6 m ³ ) and water (mineral water collected at the foot of Aso) 7
00 liters were added. This was stirred at room temperature for about 2 hours using a bamboo spatula, and allowed to stand for 3 weeks to settle the suspended fine particles, and then the supernatant of pH 2.7 was sucked with a pump and taken out. The amount of supernatant taken out was about 600 kg.

[0048] The resulting extract After pre-filtered in the back grayed filters, filter (pore size: 0.2μm, trade name: filter cartridge 0.2μm MCY444
0NFPH4, manufactured by Nippon Pall Co., Ltd., and filtered to pH
An aqueous solution containing the humus extract material of the present invention of 2.9 was obtained. Table 2 shows the properties of the obtained extract.

[0049]

[Table 2]

The obtained extract is kept at room temperature for a long time (6-12
After storage, there was almost no change.

Comparative Example 1 When the aqueous humic acid solution (stock solution) described in JP-B-62-3806 was stored at room temperature for 2 to 3 months, it turned brown. From this, it is understood that the humic acid aqueous solution described in JP-B-62-3806 is not suitable for long-term storage, and the humus-extract-containing substance-containing aqueous liquid of the present invention is superior in storability.

Experimental Example 1 (Antioxidant action) A liquid was prepared by mixing the extract obtained in Example 1 and purified water in a mixing ratio shown in Table 3. F to each 15 ml
0.05 g of eSO ₄ .7H ₂ O was added and dissolved (liquid color: colorless). After leaving this at room temperature for 50 days, the change of the liquid was observed. The results are shown in Table 3. Compared with the liquid A, a yellow color liquid B, and that the color of the liquid C and liquid D were substantially colorless and colorless respectively, FeSO ₄
It can be seen that the oxidation of is prevented.

[0053]

[Table 3]

Experimental Example 2 (anticorrosion action) The extract obtained in Example 1 as it was (undiluted solution) was used to prepare three kinds of solutions, a solution diluted 10 times with purified water and a solution diluted 50 times. Take 250 ml of each of the 3 types of liquids, put nails (10 cm in length, made of iron) into each, and cool at room temperature (25
The solution was allowed to stand for 0, 20, and 40 days, and the characteristics of the liquid were examined. The pH was measured by the glass electrode method, and the redox potential was measured by the platinum electrode method. The results are shown in Table 4.

[0055]

[Table 4]

In all three cases, the pH increased and the oxidation-reduction potential decreased with the passage of time, but when the stock solution, 10-fold diluted solution and 50-fold diluted solution were compared, the oxidizing power weakened in proportion to the dilution ratio. I understand. That is, it is understood that the stock solution shows a rapid reduction in oxidizing power in the reaction with iron (inorganic substance) and then the reducing action occurs.

Further, if each solution was left for 50 days, 1
The color of the 0-fold diluted solution and the 50-fold diluted solution was dark brown, but the nail was recognizable, and the stock solution had a pale ocher color.

Comparative Example 2 Aqueous humic acid solution described in Japanese Examined Patent Publication No. 62-3806 (stock solution, p.
H2.8) in the same manner as in Experimental Example 2 at room temperature
When left for one day, no progress of corrosion was observed for the first 3 days, but from around the 7th day, the entire aqueous solution became dark brown, and the progress of corrosion became faster. It was invisible. From this fact
It can be said that the humic acid extract-containing aqueous solution of the present invention has a better anticorrosion action than the humic acid aqueous solution described in JP-A-3806.

Experimental Example 3 (anticorrosion action) Tap water, electrolytic oxidized water and electrolytic reduced water (each having a pH of 6.) obtained from ion exchange equipment for household use (trade name: Trim Ion TI-7000, manufactured by Nippon Trim Co., Ltd.). 3 and pH 8.2), commercially available alkaline water (pH 9.1, trade name: Seiryu no water, manufactured by Seiryu), and the extract obtained in Example 1 6 times and 21 times with tap water. A push pin and a clip were put in each of the six vials containing the diluted aqueous solution, and left at room temperature (25 ° C.) for 50 days to examine the degree of corrosion. The results are shown in Table 5.

[0060]

[Table 5]

Corrosion progressed remarkably in tap water and electrolytically oxidized water, and almost no reducing action was observed in electrolytically reduced water. Also, it is considered difficult to stop the progress of corrosion even with commercially available alkaline water. When compared with these, when the diluted solution of the extract obtained in Example 1 was used, the corrosion stopped after a certain amount of corrosion had occurred. Therefore, it can be seen that the anticorrosion effect is excellent.

Experimental Example 4 (antibacterial / bactericidal action) As test bacteria, Escherichia coli IFO 3972 and Staphylococcus aureus IFO 1273
2) was used. The NA strain (standard agar medium (manufactured by Eiken Chemical Co., Ltd.)) was cultivated at 35 ° C. for 16 to 24 hours, and the test strain was inoculated again to the NA medium and cultured at 35 ° C. for 16 to 20 hours. The bacterial cells were uniformly dispersed in purified water to prepare a bacterial solution in which the number of bacterial cells per ml was about 10 ⁷ .

Extraction liquid (stock solution) 100 obtained in Example 1
Add 1 ml of the above bacterial solution to ml and store at 25 ° C.
The number of viable cells after 4 hours and 48 hours was measured by an agar plate culture method (35 ° C., 2 days culture) using SA medium (standard agar medium (Eiken Chemical Co., Ltd.)). Further, as a control, the same test was carried out with a phosphate buffer solution inoculated with the bacterial solution. The results are shown in Table 6.

[0064]

[Table 6]

In both Escherichia coli and Staphylococcus aureus, the viable cell count was close to 0 after 24 hours and 0 after 48 hours, indicating that this extract has an excellent bactericidal effect. .

Experimental Example 5 (In vitro AIDS virus inactivating action) A virus solution was prepared using AIDS virus (HIV-1 (IIIB strain)) and fetal bovine serum so that the serum concentration was 10%. . An equal amount of each of the virus solution, the extract obtained in Example 1, distilled water, and the culture solution (basic medium consisting of low-molecular-weight nutrients, balanced salts, and buffer system to which bovine serum was added) By mixing, three types of mixed solutions were obtained.

The above mixture was left at room temperature (20 ° C.) and
After 30 minutes, 30 minutes, and 120 minutes, each was diluted with the culture medium to various dilution ratios shown in Table 7. Using the resulting diluted solution, MT-4 cells (bovine-derived lymphocytes that are easily infected with HIV) were infected with AIDS virus, and then the cells were observed under a microscope on the 4th day to give a mixture solution. The amount of infectious virus in each strain was quantified.

The infectious titer represents the titer (strength) of the virus by using the biological activity as an index, and the minimum amount of the viral liquid that can normally infect a susceptible host to cause the onset is the unit price. Here, the infection unit (CC
It is shown by the number of ID ₅₀ ). CCID ₅₀ is a unit of viral infectivity, but which may be considered to represent the 1CCID ₅₀ approximately one infectious particle. The results are shown in Table 7.

[0069]

[Table 7]

In an equal volume mixture of the virus liquid and the extract,
A decrease in virus infectivity was already observed after 0 minutes of standing, and 98.6% of the virus was inactivated after 30 minutes, and after 120 minutes, the detection limit (1000 CCID ₅₀ /
ml) was inactivated to below. However, at a dilution ratio of 20 times, it was difficult to accurately determine the virus growth because it had an effect on the cell growth (* in the table).
**).

Experimental Example 6 (Cholesterol / lipolytic action) A slightly obese but healthy man (blood cholesterol level 3
(More than 00mg / dl, about 280mg / dl of neutral fat)
The extract obtained in Example 1 was diluted 10-fold (p
H3.65) was taken daily in an amount of 300 to 500 cc for 1 to 2 months. After the test period, the cholesterol level was reduced to 150 to 170 mg / dl and the neutral fat was also reduced to about 100 mg / dl in all the subjects. On the other hand, for comparison, a 10-fold diluted humic acid aqueous solution stock solution described in JP-B-62-3806 (pH)
When the same test was performed on another 5 persons with similar symptoms using 3.65), the cholesterol level was 250 to 30.
It was 0 mg / dl, and the neutral fat was also 230 to 250 mg / dl.

Since the aqueous liquid containing the humus soil extract of the present invention has not been heat-treated, it contains lipase, which shows that it has a cholesterol / lipolytic action. On the other hand, the heat-treated product had no cholesterol / lipolytic action because amino acids and proteins were destroyed by the heat of 55 ° C. or higher and the lipase lost its activity.

Experimental Example 7 (Effects on neuralgia, gout, and rheumatism) Six men and women (50 to 65 years old) with joint pain, gout, and rheumatism were diluted 10 times with the extract obtained in Example 1 ( pH 3.65) was drunk daily in an amount of 500 to 1000 cc for 60 days. It has been difficult to walk for about 8 years due to pain in the knee joints after drinking 63
The old man had no change for the first 30-45 days, but after the 50th day, the pain began to disappear, and thereafter, the pain did not occur and he became able to walk daily. . Also, a 56-year-old woman who had gout for 10 years and was so painful that she could not walk without slippers,
The pain was already gone 4 days after drinking, and walking was possible 7 days later, and the pain did not recur thereafter. A woman suffering from rheumatism was already painless 4 days after drinking, was able to walk one week later, and had no recurrence of pain thereafter. All were well, except only one 65-year-old man with rheumatism had some relief.

On the other hand, for comparison, a 10-fold diluted humic acid solution stock solution described in JP-B-62-3806 (p.
The same test was performed on 6 other patients with similar symptoms using H3.6), but only one man had some pain after 1 month, but otherwise, even after drinking for 60 days. The symptoms were the same as before drinking.

From this, it is understood that the humus-extract-containing aqueous liquid of the present invention is effective for neuralgia, rheumatism, gout, etc. due to the large enzymatic activity of urease and lipase.

Use Example 1 (Production of Body Soap) The extract obtained in Example 1 was added to commercially available body soap (trade name: Tears of Flora, manufactured by Clover Co., Ltd.).
Blended in a proportion of ˜6% to obtain a body soap that is weakly acidic and has antiseptic properties.

When the obtained body soap was used, the skin was mild to the touch and moisturized. By using the humus-extract-containing substance-containing aqueous liquid of the present invention, a weakly acidic product can be easily produced without using any preservative.

Use Example 2 (Production of beauty essence) The extract obtained in Example 1 was mixed with a commercially available beauty essence (trade name: Lermouth, Taiyo Pharmaceutical Co., Ltd.) at a ratio of 6% to obtain an extract. A containing serum was obtained. When this beauty essence was applied to sensitive skin, atopic constitution, allergic constitution and skin suppurated by acne of 18 to 30 years old, redness of the face peculiar to acne skin, allergic skin and atopic skin disappeared and itching disappeared.

Use Example 3 (Production of Shampoo) The extract obtained in Example 1 was used as a commercial shampoo (trade name: Ken Ramad Shampoo, manufactured by Cosmebose Co., Ltd.)
Was mixed with 5% to obtain a weakly acidic shampoo. When shampooing was performed using this, results such as shiny hair and firmness of hair were obtained.

Use Example 4 (Production of Diet Water) The extract obtained in Example 1 is appropriately diluted 2 to 10 times, and 30 to 50 cc per day is drunk for 30 days to give 1 to 1 month. A 2 kg weight loss was seen (4 out of 5 cases). However, when the amount of extract is small (3
(Less than 0 cc), no significant weight loss effect was observed. Therefore, it seems that the effect appears in proportion to the amount of the undiluted solution.

[0081]

The aqueous liquid containing the humus extract substance of the present invention is
Since it can be produced without heat sterilization, it can be easily produced. In addition, since humic acid contained in humus and various enzymes and organic substances are present in the aqueous liquid as they are without modification, not only antibacterial and bactericidal effects but also virus inactivating and antioxidant effects. Anticorrosion,
It has beneficial effects such as internal cleansing action, lipolytic action, alcohol degrading action, surfactant action, and cholesterol degrading action. In addition, the aqueous liquid containing the humus extract material of the present invention has excellent storage stability.

─────────────────────────────────────────────────── ─── Continued Front Page (51) Int.Cl. ⁷ Identification Code FI A61K 35/02 A61K 35/02 35/60 35/60 A61P 3/00 A61P 3/00 3/06 3/06 17/00 17 / 00 31/18 31/18 B01F 17/52 B01F 17/52 C09K 15/34 C09K 15/34 17/50 17/50 K C11B 5/00 C11B 5/00 C11D 3/38 C11D 3/38 7/40 7/40 C09K 101: 00 C09K 101: 00

Claims (2)

(57) [Claims] 1. The collected humus having a diameter of about 5 to 50 cm is mixed with the humus so that the humus is mixed with the humus for half a year in the sun.
Rough drying for 1 year and 1-2 in a greenhouse
To extract the humus obtained by the two-step drying process of drying for one month by adding water to the humus, and filtering the obtained extract with a filter of 0.1 to 0.2 μm. A humus-extract-extracted-substance-containing aqueous liquid obtained from the above, which comprises a filtrate having a pH of 2.50 to 2.99 that has not been heat-sterilized. 2. The ratio of humus to water in the extraction is 1:
The humus-extract-containing substance-containing aqueous liquid according to claim 1, which is 3 to 1: 5 (weight / volume ratio).