JP3504658B2 - 新規生体高分子の製造方法 - Google Patents
新規生体高分子の製造方法Info
- Publication number
- JP3504658B2 JP3504658B2 JP50798892A JP50798892A JP3504658B2 JP 3504658 B2 JP3504658 B2 JP 3504658B2 JP 50798892 A JP50798892 A JP 50798892A JP 50798892 A JP50798892 A JP 50798892A JP 3504658 B2 JP3504658 B2 JP 3504658B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- selection
- cycle
- mixture
- mutagenesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229920001222 biopolymer Polymers 0.000 title claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- 238000000034 method Methods 0.000 claims abstract description 44
- 231100000350 mutagenesis Toxicity 0.000 claims abstract description 29
- 238000002703 mutagenesis Methods 0.000 claims abstract description 29
- 238000009826 distribution Methods 0.000 claims abstract description 26
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 26
- 239000000203 mixture Substances 0.000 claims abstract description 14
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 10
- 238000013519 translation Methods 0.000 claims abstract description 10
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 7
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 7
- 230000010076 replication Effects 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 11
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 11
- 230000035772 mutation Effects 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims description 4
- 210000002729 polyribosome Anatomy 0.000 claims description 4
- 231100000219 mutagenic Toxicity 0.000 claims description 3
- 230000003505 mutagenic effect Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000003471 mutagenic agent Substances 0.000 claims description 2
- 231100000707 mutagenic chemical Toxicity 0.000 claims description 2
- 239000002213 purine nucleotide Substances 0.000 claims description 2
- 239000002719 pyrimidine nucleotide Substances 0.000 claims description 2
- 150000003230 pyrimidines Chemical class 0.000 claims description 2
- 230000003362 replicative effect Effects 0.000 claims description 2
- 238000005457 optimization Methods 0.000 description 15
- 230000008569 process Effects 0.000 description 10
- 238000001228 spectrum Methods 0.000 description 8
- 230000014616 translation Effects 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 230000003993 interaction Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 206010069754 Acquired gene mutation Diseases 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000002427 irreversible effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 230000037439 somatic mutation Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000005094 computer simulation Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000010429 evolutionary process Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000008080 stochastic effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Ecology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4112440.5 | 1991-04-16 | ||
| DE4112440A DE4112440C1 (enExample) | 1991-04-16 | 1991-04-16 | |
| PCT/EP1992/000840 WO1992018645A1 (de) | 1991-04-16 | 1992-04-14 | Verfahren zur herstellung von neuen biopolymeren |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06506355A JPH06506355A (ja) | 1994-07-21 |
| JP3504658B2 true JP3504658B2 (ja) | 2004-03-08 |
Family
ID=6429722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP50798892A Expired - Lifetime JP3504658B2 (ja) | 1991-04-16 | 1992-04-14 | 新規生体高分子の製造方法 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0583265B1 (enExample) |
| JP (1) | JP3504658B2 (enExample) |
| AT (1) | ATE171479T1 (enExample) |
| DE (2) | DE4112440C1 (enExample) |
| WO (1) | WO1992018645A1 (enExample) |
Families Citing this family (87)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4237381C1 (de) * | 1992-11-05 | 1994-04-28 | Diagen Inst Molekularbio | Trägermaterial zur simultanen Bindung genotypischer und phänotypischer Substanzen |
| US5849545A (en) * | 1992-05-11 | 1998-12-15 | Evotec Biosystems Gmbh | Substrate material for simultaneously binding genotypic and phenotypic substances |
| DE4343591A1 (de) * | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| WO1996017056A1 (en) * | 1994-12-02 | 1996-06-06 | Institut Pasteur | Hypermutagenesis |
| EP0714980A1 (en) * | 1994-12-02 | 1996-06-05 | Institut Pasteur | Hypermutagenesis |
| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| JPH1066577A (ja) * | 1996-08-07 | 1998-03-10 | Novo Nordisk As | 核酸プールおよびその製造方法 |
| JP3692542B2 (ja) | 1997-01-21 | 2005-09-07 | ザ ジェネラル ホスピタル コーポレーション | Ｒｎａ−蛋白質の融合体を用いた蛋白質の選抜 |
| US6261804B1 (en) | 1997-01-21 | 2001-07-17 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
| US8207093B2 (en) | 1997-01-21 | 2012-06-26 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| AU6820698A (en) | 1997-04-09 | 1998-10-30 | Danisco A/S | Improved method for preparing flour doughs and products made from such doughs using glycerol oxidase |
| WO1999011777A1 (en) * | 1997-09-03 | 1999-03-11 | Biovation Limited | Methods for protein screening |
| AU754377B2 (en) * | 1997-09-03 | 2002-11-14 | Merck Patent Gesellschaft Mit Beschrankter Haftung | Methods for protein screening |
| US6927025B1 (en) | 1997-09-03 | 2005-08-09 | Biovation Limited | Methods for protein screening |
| AU746786B2 (en) | 1997-12-08 | 2002-05-02 | California Institute Of Technology | Method for creating polynucleotide and polypeptide sequences |
| US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| IL140125A0 (en) | 1998-06-17 | 2002-02-10 | Maxygen Inc | Method for producing polynucleotides with desired properties |
| US6846655B1 (en) | 1998-06-29 | 2005-01-25 | Phylos, Inc. | Methods for generating highly diverse libraries |
| DK1098988T4 (da) | 1998-07-21 | 2007-09-03 | Danisco | Födevare |
| DE69941193D1 (de) | 1998-08-17 | 2009-09-10 | Bristol Myers Squibb Co | Identifizierung von verbindung-protein wechselwirkungen mit bibliotheken von gekoppelten protein-nukleinsäure-molekülen |
| DE19953854C2 (de) | 1999-11-09 | 2002-01-17 | Max Planck Gesellschaft | Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften |
| US7022479B2 (en) | 2000-01-24 | 2006-04-04 | Compound Therapeutics, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
| AU783689B2 (en) | 2000-01-24 | 2005-11-24 | Adnexus Therapeutics, Inc. | Sensitive, multiplexed diagnostic assays for protein analysis |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
| US7582423B2 (en) | 2001-02-02 | 2009-09-01 | Novici Biotech Llc | Population of polynucleotide sequence variants |
| AU2002314712C1 (en) | 2001-02-02 | 2008-10-02 | Novici Biotech Llc | A method of increasing complementarity in a heteroduplex polynucleotide |
| US7838219B2 (en) | 2001-02-02 | 2010-11-23 | Novici Biotech Llc | Method of increasing complementarity in a heteroduplex |
| BR0209154A (pt) | 2001-05-18 | 2004-07-20 | Danisco | Processo de preparação de uma massa com uma enzima |
| EP1840211A1 (en) | 2001-10-31 | 2007-10-03 | Danisco A/S | Pyranosone dehydratase from phanerochaete chrysosporium |
| US7078211B2 (en) | 2002-02-01 | 2006-07-18 | Large Scale Biology Corporation | Nucleic acid molecules encoding endonucleases and methods of use thereof |
| DK1504098T4 (da) | 2002-05-17 | 2011-05-23 | Alligator Bioscience Ab | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion |
| WO2004006883A1 (en) * | 2002-07-17 | 2004-01-22 | Unilever Plc | Skin care cosmetic compositions containing carboxyalkylates of branched alcohols and/or alkoxylates thereof |
| US20050196766A1 (en) | 2003-12-24 | 2005-09-08 | Soe Jorn B. | Proteins |
| MXPA05007654A (es) | 2003-01-17 | 2005-09-30 | Danisco | Metodo. |
| US7955814B2 (en) | 2003-01-17 | 2011-06-07 | Danisco A/S | Method |
| DK2278015T3 (da) | 2003-01-17 | 2014-10-13 | Dupont Nutrition Biosci Aps | Fremgangsmåde til fremstilling af en carbohydratester |
| US7335504B2 (en) | 2003-06-18 | 2008-02-26 | Direvo Biotechnology Ag | Engineered enzymes and uses thereof |
| GB0716126D0 (en) | 2007-08-17 | 2007-09-26 | Danisco | Process |
| US7718408B2 (en) | 2003-12-24 | 2010-05-18 | Danisco A/S | Method |
| AU2004312215B2 (en) | 2003-12-24 | 2009-03-05 | Dupont Nutrition Biosciences Aps | Proteins |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| CN1984982A (zh) | 2004-07-16 | 2007-06-20 | 丹尼斯科公司 | 用酶使油脱胶的方法 |
| GB0421598D0 (en) | 2004-09-29 | 2004-10-27 | Cambridge Advanced Tech | Modification of plant development and morphology |
| GB0422052D0 (en) | 2004-10-04 | 2004-11-03 | Dansico As | Enzymes |
| GB0423139D0 (en) | 2004-10-18 | 2004-11-17 | Danisco | Enzymes |
| EP2465929B1 (en) | 2006-12-14 | 2015-12-02 | DSM IP Assets B.V. | R-HNL random variants and their use for preparing optically pure, sterically hindered cyanohydrins |
| PL2405007T3 (pl) | 2007-01-25 | 2014-04-30 | Dupont Nutrition Biosci Aps | Wytwarzanie acylotransferazy lipidowej z przekształconych komórek gospodarza Bacillus licheniformis |
| US20080220498A1 (en) | 2007-03-06 | 2008-09-11 | Cervin Marguerite A | Variant Buttiauxella sp. phytases having altered properties |
| US8143046B2 (en) | 2007-02-07 | 2012-03-27 | Danisco Us Inc., Genencor Division | Variant Buttiauxella sp. phytases having altered properties |
| DK2147104T3 (en) | 2007-05-21 | 2015-12-14 | Danisco Us Inc | USING A ASPARAGINPROTEASE- (NSP24) SIGNAL SEQUENCE FOR heterologous protein EXPRESSION |
| WO2009129489A2 (en) | 2008-04-18 | 2009-10-22 | Danisco Us Inc., Genencor Division | Buttiauxella sp. phytase variants |
| EP2279248B1 (en) | 2008-04-30 | 2015-04-01 | DuPont Nutrition Biosciences ApS | Proteins |
| GB0908770D0 (en) | 2009-04-24 | 2009-07-01 | Danisco | Method |
| US20120058222A1 (en) | 2009-05-19 | 2012-03-08 | Danisco A/S | Use |
| AR077042A1 (es) | 2009-06-12 | 2011-07-27 | Danisco | Metodo para tratar una composicion que contiene pirofiofitina |
| GB0920089D0 (en) | 2009-11-17 | 2009-12-30 | Danisco | Method |
| CA2786201C (en) | 2010-01-13 | 2017-01-17 | Basf Se | Novel mannanase variants |
| CA2788148C (en) | 2010-03-12 | 2018-03-13 | Dupont Nutrition Biosciences Aps | An enzymatic process for refining a plant oil |
| WO2011114251A1 (en) | 2010-03-18 | 2011-09-22 | Danisco A/S | Foodstuff |
| WO2011158203A1 (en) | 2010-06-17 | 2011-12-22 | Danisco A/S | Process |
| GB201011513D0 (en) | 2010-07-08 | 2010-08-25 | Danisco | Method |
| AR085251A1 (es) | 2011-02-23 | 2013-09-18 | Danisco | Proceso para tratar aceite vegetal |
| AR085253A1 (es) | 2011-02-23 | 2013-09-18 | Danisco | Metodo para producir una clorofilasa |
| AR085250A1 (es) | 2011-02-23 | 2013-09-18 | Danisco | Proceso para tratar un aceite vegetal |
| KR20140024365A (ko) | 2011-04-08 | 2014-02-28 | 다니스코 유에스 인크. | 조성물 |
| HRP20180607T1 (hr) | 2011-06-10 | 2019-04-05 | Oregon Health & Science University | Glikoproteini i rekombinantni vektori cmv |
| WO2013104659A2 (en) | 2012-01-13 | 2013-07-18 | Dupont Nutrition Biosciences Aps | Process |
| WO2013104660A1 (en) | 2012-01-13 | 2013-07-18 | Dupont Nutrition Biosciences Aps | Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation |
| WO2013160374A1 (en) | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling |
| WO2013160372A1 (en) | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme |
| WO2014063097A1 (en) | 2012-10-19 | 2014-04-24 | Danisco Us Inc. | Stabilization of biomimetic membranes |
| EP3083936B1 (en) | 2013-12-19 | 2018-07-04 | Danisco US Inc. | Use of hydrophobins to increase gas transfer in aerobic fermentation processes |
| WO2015150372A1 (en) | 2014-04-01 | 2015-10-08 | Dupont Nutrition Biosciences Aps | Method for increasing crude palm oil yields |
| AU2015334890B2 (en) | 2014-10-24 | 2021-12-23 | Dupont Nutrition Biosciences Aps | Proline tolerant tripeptidyl peptidases and uses thereof |
| EP3172329A1 (en) | 2014-10-24 | 2017-05-31 | Danisco US Inc. | Method for producing alcohol by use of a tripeptidyl peptidase |
| JP2018518976A (ja) | 2015-06-26 | 2018-07-19 | デュポン ニュートリション バイオサイエンシス エーピーエス | タンパク質加水分解用アミノペプチダーゼ |
| EP3420097A1 (en) | 2016-02-25 | 2019-01-02 | DuPont Nutrition Biosciences ApS | Method for producing a protein hydrolysate employing an aspergillus fumigatus tripeptidyl peptidase |
| DE102023001827A1 (de) | 2023-04-19 | 2024-10-24 | Horst Wochnowski | Verfahren und dazugehörige Vorrichtung zum Züchten von in Gewässern Mikroplastik zersetzenden und abbauenden Mikroorganismenstämme durch einen künstlich herbeigeführten und beschleunigten Evolutions- und Selektionsprozess |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0285123A3 (en) * | 1987-04-03 | 1989-02-01 | Stabra AG | A method for complete mutagenesis of nucleic acids |
| JP3127158B2 (ja) * | 1989-10-05 | 2001-01-22 | オプテイン，インコーポレイティド | 新規の遺伝子及びポリペチドの無細胞合成並びに単離 |
| AU8498091A (en) * | 1990-08-02 | 1992-03-02 | Regents Of The University Of Colorado, The | Systematic polypeptide evolution by reverse translation |
-
1991
- 1991-04-16 DE DE4112440A patent/DE4112440C1/de not_active Expired - Lifetime
-
1992
- 1992-04-14 JP JP50798892A patent/JP3504658B2/ja not_active Expired - Lifetime
- 1992-04-14 WO PCT/EP1992/000840 patent/WO1992018645A1/de not_active Ceased
- 1992-04-14 DE DE59209506T patent/DE59209506D1/de not_active Expired - Lifetime
- 1992-04-14 AT AT92908537T patent/ATE171479T1/de not_active IP Right Cessation
- 1992-04-14 EP EP92908537A patent/EP0583265B1/de not_active Expired - Lifetime
Non-Patent Citations (2)
| Title |
|---|
| Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，１９８８年，８５，５９１３−５９１７ |
| Ｓｃｉｅｎｃｅ，１９９０年 ８月，２４９，５０５−５１０ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06506355A (ja) | 1994-07-21 |
| ATE171479T1 (de) | 1998-10-15 |
| WO1992018645A1 (de) | 1992-10-29 |
| DE4112440C1 (enExample) | 1992-10-22 |
| EP0583265A1 (de) | 1994-02-23 |
| EP0583265B1 (de) | 1998-09-23 |
| DE59209506D1 (de) | 1998-10-29 |
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