JP3504658B2 - 新規生体高分子の製造方法 - Google Patents

新規生体高分子の製造方法

Info

Publication number
JP3504658B2
JP3504658B2 JP50798892A JP50798892A JP3504658B2 JP 3504658 B2 JP3504658 B2 JP 3504658B2 JP 50798892 A JP50798892 A JP 50798892A JP 50798892 A JP50798892 A JP 50798892A JP 3504658 B2 JP3504658 B2 JP 3504658B2
Authority
JP
Japan
Prior art keywords
nucleic acid
selection
cycle
mixture
mutagenesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP50798892A
Other languages
English (en)
Japanese (ja)
Other versions
JPH06506355A (ja
Inventor
カルシュテン ヘンコ
マンフレット アイゲン
Original Assignee
エフォテック バイオジステームス アクチエンゲゼルシャフト
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6429722&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP3504658(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by エフォテック バイオジステームス アクチエンゲゼルシャフト filed Critical エフォテック バイオジステームス アクチエンゲゼルシャフト
Publication of JPH06506355A publication Critical patent/JPH06506355A/ja
Application granted granted Critical
Publication of JP3504658B2 publication Critical patent/JP3504658B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP50798892A 1991-04-16 1992-04-14 新規生体高分子の製造方法 Expired - Lifetime JP3504658B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4112440A DE4112440C1 (enExample) 1991-04-16 1991-04-16
DE4112440.5 1991-04-16
PCT/EP1992/000840 WO1992018645A1 (de) 1991-04-16 1992-04-14 Verfahren zur herstellung von neuen biopolymeren

Publications (2)

Publication Number Publication Date
JPH06506355A JPH06506355A (ja) 1994-07-21
JP3504658B2 true JP3504658B2 (ja) 2004-03-08

Family

ID=6429722

Family Applications (1)

Application Number Title Priority Date Filing Date
JP50798892A Expired - Lifetime JP3504658B2 (ja) 1991-04-16 1992-04-14 新規生体高分子の製造方法

Country Status (5)

Country Link
EP (1) EP0583265B1 (enExample)
JP (1) JP3504658B2 (enExample)
AT (1) ATE171479T1 (enExample)
DE (2) DE4112440C1 (enExample)
WO (1) WO1992018645A1 (enExample)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849545A (en) * 1992-05-11 1998-12-15 Evotec Biosystems Gmbh Substrate material for simultaneously binding genotypic and phenotypic substances
DE4237381C1 (de) * 1992-11-05 1994-04-28 Diagen Inst Molekularbio Trägermaterial zur simultanen Bindung genotypischer und phänotypischer Substanzen
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1996017056A1 (en) * 1994-12-02 1996-06-06 Institut Pasteur Hypermutagenesis
EP0714980A1 (en) * 1994-12-02 1996-06-05 Institut Pasteur Hypermutagenesis
US6406723B1 (en) 1997-04-09 2002-06-18 Danisco A/S Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
JPH1066577A (ja) * 1996-08-07 1998-03-10 Novo Nordisk As 核酸プールおよびその製造方法
US6261804B1 (en) 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions
IL131016A (en) 1997-01-21 2004-12-15 Gen Hospital Corp Selection of proteins using RNA-protein blends
US8207093B2 (en) 1997-01-21 2012-06-26 The General Hospital Corporation Selection of proteins using RNA-protein fusions
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
JP4524445B2 (ja) * 1997-09-03 2010-08-18 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング タンパク質のスクリーニング方法
US6927025B1 (en) 1997-09-03 2005-08-09 Biovation Limited Methods for protein screening
AU754377B2 (en) * 1997-09-03 2002-11-14 Merck Patent Gesellschaft Mit Beschrankter Haftung Methods for protein screening
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
JP2002517995A (ja) * 1998-06-17 2002-06-25 マキシジェン, インコーポレイテッド 所望の特性を有するポリヌクレオチドを生成するための方法
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
ATE231186T1 (de) 1998-07-21 2003-02-15 Danisco Lebensmittel
DK1105360T3 (da) 1998-08-17 2009-11-02 Bristol Myers Squibb Co Identifikation af forbindelse-protein-interaktioner ved anvendelse af biblioteker af protein-nukleinsyrefusionsmolekyler
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
EP1250463B1 (en) 2000-01-24 2006-04-05 Compound Therapeutics, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
US7022479B2 (en) 2000-01-24 2006-04-04 Compound Therapeutics, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
ATE447019T1 (de) 2001-02-02 2009-11-15 Large Scale Biology Corp Methode zur erhöhung der komplementarität in einem heteroduplex-polynucleotid
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US7838219B2 (en) 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
WO2002094123A2 (en) 2001-05-18 2002-11-28 Danisco A/S Method of preparing a dought with an enzime
EP1840211A1 (en) 2001-10-31 2007-10-03 Danisco A/S Pyranosone dehydratase from phanerochaete chrysosporium
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
ATE356871T1 (de) 2002-05-17 2007-04-15 Alligator Bioscience Ab Eine methode zur in vitro molekularen evolution der proteinfunktion
JP2005539093A (ja) * 2002-07-17 2005-12-22 ユニリーバー・ナームローゼ・ベンノートシヤープ 分枝状アルコールのカルボキシアルキレート及び/またはそのアルコキシレートを含有するスキンケア用化粧組成物
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
EP2290085B1 (en) 2003-01-17 2017-12-13 DuPont Nutrition Biosciences ApS Method for the in situ production of an emulsifier in a foodstuff
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
EP1633865B1 (en) 2003-06-18 2011-09-28 Bayer Pharma Aktiengesellschaft New biological entities and the use thereof
CA2550800A1 (en) 2003-12-24 2005-07-21 Danisco A/S Methods to produce variant glycolipid acyltransferases and variant glycolipid acyltransferases with enhanced activity towards glycolipids.
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
CN102533440B (zh) 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
GB0422052D0 (en) 2004-10-04 2004-11-03 Dansico As Enzymes
GB0423139D0 (en) 2004-10-18 2004-11-17 Danisco Enzymes
EP2465929B1 (en) 2006-12-14 2015-12-02 DSM IP Assets B.V. R-HNL random variants and their use for preparing optically pure, sterically hindered cyanohydrins
CA2673954C (en) 2007-01-25 2015-09-15 Danisco A/S Production of a lipid acyltransferase from transformed bacillus licheniformis cells
US20080220498A1 (en) 2007-03-06 2008-09-11 Cervin Marguerite A Variant Buttiauxella sp. phytases having altered properties
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
DK2147104T3 (en) 2007-05-21 2015-12-14 Danisco Us Inc USING A ASPARAGINPROTEASE- (NSP24) SIGNAL SEQUENCE FOR heterologous protein EXPRESSION
US8673609B2 (en) 2008-04-18 2014-03-18 Danisco Us Inc. Buttiauxella sp. phytase variants
WO2009133464A2 (en) 2008-04-30 2009-11-05 Danisco A/S Oxidation process
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
EP2432876A1 (en) 2009-05-19 2012-03-28 Danisco A/S Use
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
ES2575917T3 (es) 2010-01-13 2016-07-04 Basf Se Nuevas variantes de mananasa
CN102844418B (zh) 2010-03-12 2015-11-25 杜邦营养生物科学有限公司 方法
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
EP2582809A1 (en) 2010-06-17 2013-04-24 DuPont Nutrition Biosciences ApS Process
GB201011513D0 (en) 2010-07-08 2010-08-25 Danisco Method
CA2825632C (en) 2011-02-23 2020-07-07 Dupont Nutrition Biosciences Aps Process for treating plant oil
AR085251A1 (es) 2011-02-23 2013-09-18 Danisco Proceso para tratar aceite vegetal
CN103797114A (zh) 2011-02-23 2014-05-14 杜邦营养生物科学有限公司 生产能够水解叶绿素或叶绿素衍生物的重组酶的方法
EP2694537A1 (en) 2011-04-08 2014-02-12 Danisco US Inc. Compositions
ES2667425T3 (es) 2011-06-10 2018-05-10 Oregon Health & Science University Glucoproteínas y vectores recombinantes de CMV
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
WO2014063097A1 (en) 2012-10-19 2014-04-24 Danisco Us Inc. Stabilization of biomimetic membranes
DK3083936T3 (en) 2013-12-19 2018-10-22 Danisco Us Inc USE OF HYDROPHOBINES TO INCREASE GAS TRANSFER IN AEROBE FERMENTATION PROCESSES
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
CN107105707B (zh) 2014-10-24 2022-07-22 杜邦营养生物科学有限公司 脯氨酸耐受性三肽基肽酶及其用途
EP3172329A1 (en) 2014-10-24 2017-05-31 Danisco US Inc. Method for producing alcohol by use of a tripeptidyl peptidase
CA2990822A1 (en) 2015-06-26 2016-12-29 Dupont Nutrition Biosciences Aps Aminopeptidases for protein hydrlyzates
MX2018009216A (es) 2016-02-25 2018-11-09 Dupont Nutrition Biosci Aps Metodo para producir un hidrolizado de proteina empleando una tripeptidil peptidasa de aspergillus fumigatus.
DE102023001827A1 (de) 2023-04-19 2024-10-24 Horst Wochnowski Verfahren und dazugehörige Vorrichtung zum Züchten von in Gewässern Mikroplastik zersetzenden und abbauenden Mikroorganismenstämme durch einen künstlich herbeigeführten und beschleunigten Evolutions- und Selektionsprozess

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0285123A3 (en) * 1987-04-03 1989-02-01 Stabra AG A method for complete mutagenesis of nucleic acids
ES2118066T3 (es) * 1989-10-05 1998-09-16 Optein Inc Sintesis y aislamiento, exentos de celulas, de nuevos genes y polipeptidos.
WO1992002536A1 (en) * 1990-08-02 1992-02-20 The Regents Of The University Of Colorado Systematic polypeptide evolution by reverse translation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Proc.Natl.Acad.Sci.USA,1988年,85,5913−5917
Science,1990年 8月,249,505−510

Also Published As

Publication number Publication date
EP0583265B1 (de) 1998-09-23
WO1992018645A1 (de) 1992-10-29
DE4112440C1 (enExample) 1992-10-22
ATE171479T1 (de) 1998-10-15
JPH06506355A (ja) 1994-07-21
DE59209506D1 (de) 1998-10-29
EP0583265A1 (de) 1994-02-23

Similar Documents

Publication Publication Date Title
JPH06506355A (ja) 新規生体高分子の製造方法
US5795747A (en) Process for manufacturing new biopolymers
Begik et al. Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing
JP6798056B2 (ja) Htpゲノム操作プラットフォームによる微生物株の改良
Chen et al. Single‐cell sequencing methodologies: from transcriptome to multi‐dimensional measurement
AU761570B2 (en) Methods for generating highly diverse libraries
RU96118426A (ru) Мутагенез днк за счет неупорядоченной фрагментации и вторичной сборки
WO2003002758A1 (en) Nucleic acid ligands to complex targets
Schulman et al. Recent excitement in understanding transfer RNA identity
WO2023154533A3 (en) Combinatorial dna assembly for multispecific antibodies
GB2617285A (en) Multiparametric discovery and optimization platform
Schmidlin et al. Distinguishing mutants that resist drugs via different mechanisms by examining fitness tradeoffs
EP1409666A2 (en) Process for preparing variant polynucleotides
JP2023549850A5 (enExample)
TAKITA et al. Applications of the long and accurate polymerase chain reaction method in yeast molecular biology: direct sequencing of the amplified DNA and its introduction into yeast
JP5608637B2 (ja) 4段階連続的pcr、4段階ブロックpcr、または遺伝子合成方法を用いた菌株特異的バーコードを含む遺伝子標的化ヘテロ接合分裂酵母菌株の製造方法
CN120574836A (zh) 一种同时识别新烟碱类农药吡虫啉和噻虫胺的核酸适配体及其应用
CN119265185A (zh) 一种用于对细胞进行标记的寡核苷酸与方法
CN119889461A (zh) 一种基于机器学习模型预测胞嘧啶脱氨酶功能的方法及应用
Koffas et al. Evolutionary metabolic engineering
WO2020212512A1 (en) Detection of nucleic acid modifications by using chemical deamination
Loomis et al. Developmental changes in transcriptional profiles
WO2024197857A1 (zh) 一种筛选向导rna的方法
Chen Guide to plant single-cell technology: functional genomics and crop improvement
US20230295674A1 (en) Method for screening target gene using crispri system and uses thereof

Legal Events

Date Code Title Description
A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20031211

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20071219

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20081219

Year of fee payment: 5

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20081219

Year of fee payment: 5

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20081219

Year of fee payment: 5

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20091219

Year of fee payment: 6

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101219

Year of fee payment: 7

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101219

Year of fee payment: 7

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

Free format text: JAPANESE INTERMEDIATE CODE: R313111

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20101219

Year of fee payment: 7

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20111219

Year of fee payment: 8

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121219

Year of fee payment: 9

EXPY Cancellation because of completion of term
FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20121219

Year of fee payment: 9