JP3090692B2 - 酵素反応測定方法及びその測定装置 - Google Patents
酵素反応測定方法及びその測定装置Info
- Publication number
- JP3090692B2 JP3090692B2 JP53078397A JP53078397A JP3090692B2 JP 3090692 B2 JP3090692 B2 JP 3090692B2 JP 53078397 A JP53078397 A JP 53078397A JP 53078397 A JP53078397 A JP 53078397A JP 3090692 B2 JP3090692 B2 JP 3090692B2
- Authority
- JP
- Japan
- Prior art keywords
- solution
- enzyme
- reaction
- measurement
- prism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000006911 enzymatic reaction Methods 0.000 title claims description 59
- 238000000034 method Methods 0.000 title claims description 24
- 238000005259 measurement Methods 0.000 claims description 91
- 238000006243 chemical reaction Methods 0.000 claims description 75
- 239000000758 substrate Substances 0.000 claims description 69
- 238000010521 absorption reaction Methods 0.000 claims description 49
- 102000004190 Enzymes Human genes 0.000 claims description 48
- 108090000790 Enzymes Proteins 0.000 claims description 48
- 238000001228 spectrum Methods 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 19
- 238000004140 cleaning Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 16
- 238000000862 absorption spectrum Methods 0.000 claims description 14
- 239000004367 Lipase Substances 0.000 claims description 11
- 102000004882 Lipase Human genes 0.000 claims description 11
- 108090001060 Lipase Proteins 0.000 claims description 11
- 235000019421 lipase Nutrition 0.000 claims description 11
- 239000004809 Teflon Substances 0.000 claims description 9
- 229920006362 Teflon® Polymers 0.000 claims description 9
- 238000002835 absorbance Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 102000004157 Hydrolases Human genes 0.000 claims description 2
- 108090000604 Hydrolases Proteins 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 137
- 238000000691 measurement method Methods 0.000 description 10
- 238000004364 calculation method Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 238000012856 packing Methods 0.000 description 8
- 238000003760 magnetic stirring Methods 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 description 6
- 239000004006 olive oil Substances 0.000 description 6
- 235000008390 olive oil Nutrition 0.000 description 6
- 229920002451 polyvinyl alcohol Polymers 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000000872 buffer Substances 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000002285 radioactive effect Effects 0.000 description 4
- 238000004448 titration Methods 0.000 description 4
- 208000016222 Pancreatic disease Diseases 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 229910001873 dinitrogen Inorganic materials 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 239000003925 fat Substances 0.000 description 3
- 235000019626 lipase activity Nutrition 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 239000012089 stop solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 2
- 235000019799 monosodium phosphate Nutrition 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 125000004185 ester group Chemical group 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000002314 glycerols Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000012933 kinetic analysis Methods 0.000 description 1
- 150000004668 long chain fatty acids Chemical class 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4180596 | 1996-02-28 | ||
| JP8-41805 | 1996-02-28 | ||
| PCT/JP1997/000513 WO1997032198A1 (en) | 1996-02-28 | 1997-02-24 | Method and apparatus for assaying enzymatic reaction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPWO1997032198A1 JPWO1997032198A1 (ja) | 1999-01-12 |
| JP3090692B2 true JP3090692B2 (ja) | 2000-09-25 |
Family
ID=12618555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP53078397A Expired - Fee Related JP3090692B2 (ja) | 1996-02-28 | 1997-02-24 | 酵素反応測定方法及びその測定装置 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US5905030A (enExample) |
| EP (1) | EP0884584A1 (enExample) |
| JP (1) | JP3090692B2 (enExample) |
| WO (1) | WO1997032198A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2024031553A (ja) * | 2022-08-26 | 2024-03-07 | 東亜ディーケーケー株式会社 | 測定装置 |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4308325B2 (ja) * | 1997-02-28 | 2009-08-05 | シーフィード | 熱交換を行ない光学的に検出する化学反応アセンブリ |
| US6054711A (en) * | 1997-11-12 | 2000-04-25 | Millennium Pharmaceuticals, Inc. | Methods for identifying biological macromolecule interactions with compounds, particularly in complex mixtures |
| JP2000162124A (ja) * | 1998-12-01 | 2000-06-16 | Nippon Laser Denshi Kk | 表面プラズモン共鳴角検出装置のセンサーチップ |
| US6395538B1 (en) * | 1999-07-16 | 2002-05-28 | Human Genome Sciences, Inc. | Method and system for providing real-time, in situ biomanufacturing process monitoring and control in response to IR spectroscopy |
| CA2480739C (en) * | 2002-04-03 | 2012-12-18 | Johann Wolfgang Goethe-Universitat Frankfurt Am Main | Infrared measuring device, especially for the spectrometry of aqueous systems, preferably multiplecomponent systems |
| FR2864848B1 (fr) * | 2004-01-07 | 2006-05-26 | Patrick Prevost | Dispositif perfectionne de spectroscopie d'energie de rayonnement infra-rouge |
| US20060200070A1 (en) | 2005-02-14 | 2006-09-07 | Callicoat David N | Method and apparatus for calibrating an analyte detection system with a calibration sample |
| US8251907B2 (en) | 2005-02-14 | 2012-08-28 | Optiscan Biomedical Corporation | System and method for determining a treatment dose for a patient |
| WO2006127724A2 (en) * | 2005-05-23 | 2006-11-30 | Optiscan Biomedical Corporation | Spectroscopic analysis of a biological fluid reacted with an enzyme |
| US8597190B2 (en) | 2007-05-18 | 2013-12-03 | Optiscan Biomedical Corporation | Monitoring systems and methods with fast initialization |
| US8629399B2 (en) * | 2009-09-22 | 2014-01-14 | Bp Corporation North America Inc. | Methods and apparatuses for measuring biological processes using mid-infrared spectroscopy |
| EP2580589B1 (en) | 2010-06-09 | 2016-08-31 | Optiscan Biomedical Corporation | Measuring analytes in a fluid sample drawn from a patient |
| GB2494853B (en) * | 2011-07-11 | 2013-09-04 | M Squared Lasers Ltd | Maturation apparatus and methods |
| JP5883314B2 (ja) * | 2011-09-02 | 2016-03-15 | 日本インスツルメンツ株式会社 | 還元気化水銀測定装置 |
| GB2514387B (en) | 2013-05-22 | 2015-08-12 | M Squared Lasers Ltd | Maturation monitoring apparatus and methods |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5170056A (en) | 1991-02-28 | 1992-12-08 | Galileo Electro-Optics Corporation | Optical fiber coupled devices for remote spectroscopy in the infrared |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4022667A (en) * | 1975-09-22 | 1977-05-10 | The University Of Alabama | Substrate solution for carboxylic ester hydrolase determination |
| AT392539B (de) * | 1986-02-03 | 1991-04-25 | Avl Verbrennungskraft Messtech | Verfahren zur optischen bestimmung der katalytischen enzymaktivitaet einer probe, und eine anordnung zur durchfuehrung des verfahrens |
| JPS63263446A (ja) * | 1986-03-25 | 1988-10-31 | チバ コ−ニング ダイアグノステイクス コ−ポレ−シヨン | 無限小波センサ |
| US4893894A (en) * | 1988-04-29 | 1990-01-16 | Mine Safety Appliances Company | Evanescent sensor |
| SE462408B (sv) * | 1988-11-10 | 1990-06-18 | Pharmacia Ab | Optiskt biosensorsystem utnyttjande ytplasmonresonans foer detektering av en specific biomolekyl, saett att kalibrera sensoranordningen samt saett att korrigera foer baslinjedrift i systemet |
| JPH0560686A (ja) * | 1991-09-03 | 1993-03-12 | Kao Corp | 皮脂成分測定装置 |
| JP3011825B2 (ja) * | 1992-08-07 | 2000-02-21 | 新日本製鐵株式会社 | 油の鹸化価・酸価及び脂肪酸鉄測定方法 |
| JP3227866B2 (ja) * | 1993-02-17 | 2001-11-12 | 住友電気工業株式会社 | 硬化反応の測定方法及び装置 |
| DE69525226T2 (de) * | 1994-11-25 | 2002-06-27 | Kyoto Daiichi Kagaku Co. Ltd., Kyoto | Vorrichtung und Verfahren zur Bestimmung von Wasserstoffperoxid |
| JPH08145879A (ja) * | 1994-11-25 | 1996-06-07 | Kdk Corp | 過酸化水素の定量方法及びその装置 |
-
1997
- 1997-02-24 JP JP53078397A patent/JP3090692B2/ja not_active Expired - Fee Related
- 1997-02-24 EP EP97904619A patent/EP0884584A1/en not_active Withdrawn
- 1997-02-24 US US09/077,928 patent/US5905030A/en not_active Expired - Fee Related
- 1997-02-24 WO PCT/JP1997/000513 patent/WO1997032198A1/ja not_active Ceased
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5170056A (en) | 1991-02-28 | 1992-12-08 | Galileo Electro-Optics Corporation | Optical fiber coupled devices for remote spectroscopy in the infrared |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2024031553A (ja) * | 2022-08-26 | 2024-03-07 | 東亜ディーケーケー株式会社 | 測定装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0884584A1 (en) | 1998-12-16 |
| WO1997032198A1 (en) | 1997-09-04 |
| US5905030A (en) | 1999-05-18 |
| EP0884584A4 (enExample) | 1998-12-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |