JP2777608B2 - Production of large resin-embedded specimens of living organisms - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial application field) The present invention relates to a method of embedding a large resin of 2 kg or more as a resin-embedded specimen of living organisms such as large plants, fishes, and internal organs of animals, using a transparent resin embedding. The present invention relates to a method for producing a large resin-embedded specimen of an organism that can be stored as a specimen for a long time.

(Prior art) It is a common practice to preserve long-lived specimens such as living organisms that can easily change in the air as transparent resin-embedded specimens, but this is also described in JP-A-61-293901. Have been.

Acrylic resin, polyester resin, urethane resin, epoxy resin, etc. used for this transparent resin embedded specimen,
Since it generates heat during curing, apart from thin plate-shaped ones, those exceeding a few hundred grams are difficult to manufacture, and the largest ones are rarely more than 2 kg. 2kg
This is because, in the case described above, the contents change due to heat generation, and in severe cases, the resin itself cracks.

Since only the silicone resin does not generate heat in the transparent resin, it can be made large, but the silicone resin is not suitable for embedded specimens of living organisms because of its high gas permeability. Therefore, silicone resin is mainly used for waterproof embedding of electrical equipment, etc., and even when used in the field of decorative products, its contents are limited to those that do not change even when left in the air, such as metallic glass products. ing.

(Problems to be Solved by the Invention) The present invention is intended to be used in large-sized plants, fishes, animal organs and other organisms in order to obtain resin-embedded specimens. It is an object of the present invention to expand the range of a resin-embedded specimen by making it easy to manufacture without causing any adverse effects due to heat generation during curing as in the case of the resin for embedding.

(Means for Solving the Problems) As a result of intensive research to solve the above-mentioned problems, the present inventor has found that a silicone resin that does not generate heat during curing is used in combination with a conventional embedding resin, so that a large size of 2 kg or more can be obtained. It has been found that a resin-embedded specimen can be easily manufactured, and the present invention has been completed.

That is, the present invention is a resin embedded specimen of 2k
When manufacturing large objects of g or more, dehydrate or fix organisms and then dry them to form an acrylic resin, polyester resin, urethane resin or epoxy resin layer on all surfaces, and then use a silicone adhesive Is placed in a mold that is a transparent and surface layer formed of a material having gas barrier properties, and a silicone adhesive is applied to the inner surface of the mold, and the mold and the treated organism And filling the cavity with silicon resin.

The organism of the present invention is a plant, such as a flower, a fish, or an internal organ of an animal, and has a large form and weighs 2 kg or more when used as a resin-embedded specimen.

In order to make the above-mentioned organism into a resin-embedded specimen, first, it is dehydrated or fixed, and then dried. To prepare a specimen of an organism, it is necessary to dehydrate or fix it in order to prevent rot and stop the activity of autolytic enzymes in the organism and suppress discoloration. In the case of a buried specimen, it must also be dried, and at least the surface alone must be dried. Drying alone has a fixing effect, but since it is not effective in preventing deformation and discoloration other than freeze-drying, it generally requires dehydration or fixing treatment.

The dehydration or fixing treatment and drying are specifically described as follows.

(A) In the case of a substance containing a large amount of water, such as a frog or the internal organs of an animal, fix it by immersing it in a 10-fold formalin solution for a day or night or several days. After washing with water, it is placed in a stream of warm dry air to dry only the surface.

(B) Fish are dehydrated and fixed by sequentially immersing in 30%, 50%, 70% of alcohol and pure alcohol. This is heated to dry the surface.

(C) Flowers are immersed in butanol to which pyridine, thiourea or zinc chloride is added to perform dehydration and fixation. This is dried by heating.

(D) Green plants such as cacti and succulents are stabilized with chlorophyll simultaneously with enzyme fixation with a copper salt solution. After washing with water, it is placed in a stream of warm dry air and only the surface is dried.

In addition to the above, examples of the fixing agent include osmic acid, picric acid, nitric acid, mercury chloride, platinum chloride, and various other types, and are appropriately selected according to the type and purpose of the organism.

After dehydration or fixing treatment as described above, a layer of an acrylic resin, a polyester resin, a urethane resin or an epoxy resin is formed on the entire surface of the dried product. This is for preliminarily coating a living organism with the above-mentioned resin having gas barrier properties because the silicone resin has a high gas permeability, and this coating can be performed by an appropriate method.

For example, in the case of the internal organs of frogs and animals described in (a) above, those obtained by drying only the surface and immediately applying a prepolymer of an acrylic resin, a polyester resin, a urethane resin or an epoxy resin to which a curing agent has been added. A cured film is formed within a short time. In the case of the fish (b),
After dehydration and fixing with alcohol, immersed in the above resin monomer to replace the alcohol with the resin monomer,
After dipping in a prepolymer of the above resin with a curing agent added,
The prepolymer taken out and soaked into the surface of the fish and the prepolymer adhered to the surface are integrally heated and polymerized to form a resin layer on the entire surface from the surface layer of the fish. In the case of the flower of (c), after heating and drying, a resin prepolymer is applied in the same manner as in (a) to form a cured film. In the case of green plants such as cactus and succulent plants in (d), a resin prepolymer is applied similarly to (a) to form a cured film.

A silicone adhesive is applied on the living body on which the cured film has been formed in order to adhere well to the silicone resin.

Separately, a transparent and air-blocking material such as acrylic resin, polyester resin, urethane resin,
Using an epoxy resin or glass or the like, a mold to be a surface layer of a large resin-embedded specimen is prepared, and a silicone adhesive is applied to the inner surface. The treated organism is put into the mold, and a silicone resin prepolymer containing a curing agent is injected into a gap between the mold and the organism, and the gap is filled with the silicone resin prepolymer and cured. Let it.

When injecting the silicone resin prepolymer, it is not always necessary to use the mold that will be the surface layer from the beginning,
In advance, create a mold with the same shape as the mold that will be the surface layer with a metal plate or resin plate, inject the silicone resin prepolymer using this, and remove the mold when the silicone resin prepolymer gels, When a void is formed in a mold serving as a surface layer, a silicone resin prepolymer is further injected.

(Effects of the Invention) According to the present invention, since the silicone resin does not generate heat when cured, a large resin-embedded specimen of 2 kg or more can be manufactured without any problem. Then, a resin layer having gas barrier properties is formed on the entire surface of the organism,
Since the surface layer of the embedded specimen is made of a resin or glass having gas barrier properties, gas infiltration is prevented regardless of the gas permeability of the silicone resin. A large specimen that can be stored for a long period is obtained.

(Example) Example 1 Shark specimen A shark is immersed in 10-fold formalin and left at room temperature for 48 hours. At the same time, formalin is injected from the anus. The content of the stomach is extremely susceptible to decay, so that it is prevented, and at the same time, formalin is quickly penetrated into thick fish.

When the fixation is completed, wash thoroughly with water and freeze-dry. When the drying is completed, the acrylic monomer is impregnated. This is to help the next prepolymer penetrate, so do not spend more time than necessary. If it gets too long, it will dissolve the pigment on the body surface of the fish and make it worse.

Next, it is immersed in a polyester prepolymer to which a curing agent has been added, and if this has permeated into the surface layer, it is immediately taken out and cured by heating. At this time, if you just lay the fish on its side, the prepolymer will drip down and only a very thin and uneven coating can be obtained, so insert a metal rod into the mouth of the fish and the bar will be horizontal In position, the fish is gently rolled in the air to form a relatively thick uniform coating in one shot. Repeat this three or four times to make a wrapped with a thick shell of 2-3 mm or more.

This is coated with a silicone adhesive and dried, then placed in a rectangular parallelepiped mold box assembled with a metal plate, and a silicone prepolymer containing a curing agent is injected into the gap. If this gelled, disassemble and remove the metal plate mold, 40 ~
Place in a dryer at 45 ° C. for 5 days to completely remove the alcohol by-produced when the silicone resin hardens.

An acrylic resin thick plate coated with a silicone adhesive is adhered to the surface of the silicon resin block to complete a sealed box.

Example 2 Specimen of Rafflesia Rafflesia, the largest flower in the plant kingdom, has a diameter of 80 to 100 cm and a height of 3
Reach 0-40cm.

The flowers are collected immediately after flowering, fixed on site with a fixing solution containing osmic acid, alcohol, and others, refrigerated, and brought back. This flower is very active in enzyme activity and rapidly turns black.

This is dehydrated by an organic solvent dehydration method, and is impregnated with cesyl alcohol while being dried in an infrared oven to produce a primary color dried flower. If this flower is left in the air, it gradually absorbs moisture and causes discoloration and deformation.Therefore, the surface is coated with an epoxy prepolymer containing a curing agent, and then a polyester prepolymer containing a curing agent. Finish like the technique of
Let it cure.

Specimens coated with this resin have considerable mechanical strength as they are, and have considerable storage stability (in air), but the surface is covered with a thick layer of light and looks quite different from the original state appear. It is embedded in a transparent silicone resin in order to improve the preservability and give a realistic appearance.

Thickness so that it can be embedded in a hemisphere with a height of 45 cm and a diameter of 90 cm
Mold with 10mm acrylic resin and apply silicone adhesive inside. On the other hand, prepare a mold made of an acrylic resin thin plate of almost the same shape, put the above treated rafflesia into it, inject silicone resin prepolymer with a hardening agent in the gap, and gel it to a soft rubbery Once it has formed, the acrylic sheet mold is removed and left at 40 ° C. for 7 days to completely cure and remove the alcohol by-product.

Next, this is inserted into the original mold. At that time, a silicone resin prepolymer to which a curing agent has been added is injected to fill the gap between the embedding product and the mold and to simultaneously adhere to the outer shell. . Finally, a thick plate of acrylic resin is adhered to the opening at the bottom and closed to complete.

Claims (3)

(57) [Claims] (1) In producing a large resin specimen of 2 kg or more as a resin-embedded specimen of an organism, the organism is dehydrated or fixed and then dried, and an acrylic resin, polyester resin, urethane resin or epoxy After forming a layer of resin and applying a silicone adhesive on it,
A transparent and gas-blocking material is placed in a mold serving as a surface layer, and a silicone adhesive is applied to the inner surface of the mold, and the void between the mold and the treated organism is filled with silicon. A method for producing a large resin-embedded specimen of an organism, characterized by filling with a resin. 2. The method for producing a large resin-embedded specimen of a living organism according to claim 1, wherein the transparent material having gas barrier properties is an acrylic resin, a polyester resin, a urethane resin or an epoxy resin. 3. The method for producing a large resin-embedded specimen according to claim 1, wherein the transparent and gas-blocking material is glass.