CN1055373C - Organism preserving technology - Google Patents
Organism preserving technology Download PDFInfo
- Publication number
- CN1055373C CN1055373C CN96116981A CN96116981A CN1055373C CN 1055373 C CN1055373 C CN 1055373C CN 96116981 A CN96116981 A CN 96116981A CN 96116981 A CN96116981 A CN 96116981A CN 1055373 C CN1055373 C CN 1055373C
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- CN
- China
- Prior art keywords
- organism
- dehydration
- silica gel
- days
- biological sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000005516 engineering process Methods 0.000 title claims description 13
- 238000000034 method Methods 0.000 claims abstract description 21
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000741 silica gel Substances 0.000 claims abstract description 12
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 11
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000002253 acid Substances 0.000 claims abstract description 6
- 150000004756 silanes Chemical class 0.000 claims abstract description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 20
- 230000018044 dehydration Effects 0.000 claims description 18
- 238000006297 dehydration reaction Methods 0.000 claims description 18
- 230000008595 infiltration Effects 0.000 claims description 13
- 238000001764 infiltration Methods 0.000 claims description 13
- 239000012472 biological sample Substances 0.000 claims description 12
- 239000002131 composite material Substances 0.000 claims description 5
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims description 5
- 229920005573 silicon-containing polymer Polymers 0.000 claims description 5
- 238000005470 impregnation Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000000740 bleeding effect Effects 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 abstract 2
- 229910052710 silicon Inorganic materials 0.000 abstract 2
- 239000010703 silicon Substances 0.000 abstract 2
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 239000004205 dimethyl polysiloxane Substances 0.000 abstract 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 abstract 1
- 239000000499 gel Substances 0.000 abstract 1
- 231100000957 no side effect Toxicity 0.000 abstract 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 abstract 1
- 238000007493 shaping process Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 description 6
- 238000007710 freezing Methods 0.000 description 6
- 210000003734 kidney Anatomy 0.000 description 4
- 238000005238 degreasing Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 239000013078 crystal Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a new technique for preserving organisms, which comprises the steps of fixing an organism, dewatering the organism, infiltrating the organism in vacuum, hardening the organism and shaping the organism. All the steps are carried out at normal temperature; the step of dewatering the organism is carried out through gradual degradation, the step of infiltrating the organism in vacuum is carried out in biological plasticized silica gel solution, and the biological plasticized silicon gel solution is prepared from polydimethyl siloxane, chloroplatnic acid, compounded organic silicon, modified silane and aether; and finally, the organism is prompted to be naturally hardened and shaped with organoalkoxysilane. The traditional method by which organism can be stored for a long period of time only in formalin solution, and the original technique which needs to be carried out at low temperature is changed. The new technique is carried out at normal temperature and has the advantages of reduced cost, greatly extended storage life and no side effect.
Description
What the present invention relates to is a kind of biological new technology of preserving that is used for.
The freezing cryogenic conditions of German Hagens is generally adopted in present each biological plasticized laboratory in the world, dehydration is freezing low temperature (25-30 ℃) evaporation, vacuum infiltration also carries out under-25～30 ℃ cryogenic conditions, therefore carry out the essential low temperature refrigerator of buying earlier of biological plasticized technology, investment large-scale low-temperature freezing equipment, whole plasticizing process needs long with the time, is generally 3-4 about the month.Ancient hydrometer method is then adopted in the test of acetone concentration in the dehydration, has not only bothered time-consumingly but also dangerous, can not judge the progress of dehydration fast.The vacuum infiltration process adopts and reaches 30-40 days continuous air extraction method, and vacuum equipment (especially vavuum pump) is required height, and it is many to expend the energy, and workload is big, and safety coefficient is low.Monitoring to vacuum infiltration process progress is then judged with the perusal acetone bubble situation of overflowing entirely.
The objective of the invention is to overcome the defective of above-mentioned existence, propose a kind of biological making new technology of preserving of under normal temperature condition, finishing.
Technical solution of the present invention:
Divide (1), fix, (2), dehydration, (3), vacuum infiltration, (4), hardened forming technology, wherein (1), technique for fixing are that biological sample was put into 5% formalin solution fixedly 10-20 days at normal temperatures, (2), dewatering process is that biological sample after will fixing at normal temperatures is placed in the acetone soln of different content and carries out the classification dehydration, promptly respectively dewatered in 50%, 60%, 70%, 80%, 90%, 95%, 97%, 98%, 99% acetone soln two days, dehydration is seven days in 100% acetone soln; (3), vacuum impregnation technique is with the biological sample after the dehydration at normal temperatures, input is in biological plasticized silica gel solution, adopt the discontinuous negative pressure of vacuum infiltration method of bleeding, biological plasticized silica gel is fully infiltrated, time 10-25 days, this biological plasticized silica gel composition was made up of dimethyl silicone polymer, chloroplatinic acid, composite organic modified silane, ether; (4) hardened forming technology, under normal temperature, the condition of humidity at 60-70%, unsettled shelving used the organoalkoxysilane air-set with the biological sample after fixing, dehydration, the infiltration.
Its each composition weight of biological plasticized silica gel in vacuum impregnation technique is respectively: get the 100kg example with dimethyl silicone polymer, get chloroplatinic acid 1-5kg, get composite organic modified silane 2-8kg, get ether 5-10kg, the organoalkoxysilane that is used for hardened forming technology is got 0-2kg.
Advantage of the present invention:
1. four biological plasticized basic steps (fixing, dehydration, vacuum infiltration, hardened forming) are all carried out at ambient temperature, have saved-25～30 ℃ of necessary freezing Cryo Equipments of cryogenic conditions.Thereby cost of investment is reduced significantly, and can shorten whole fusion time (from the 3-4 month shortening to the 2-3 month)
2. the progressively evaporation under the room temperature condition can guarantee that sample inside can not produce the cyto-architectural ice crystal of disorganize, and dehydrating effect is better than freezing low temperature dewatering method, (shrinkage is about 10%) dewatering time short (2-3 is about week).In addition at ambient temperature, dehydration is carried out simultaneously with degreasing, can save the essential additional degreasing time of freezing low temperature dewatering, thereby can shorten whole biological plasticized process time 1-2 week.
Embodiment:
Get kidney (human body)
(1) put into formalin solution (300ml), fix 10 days, normal temperature condition,
(2) kidney after fixing is put into 50%, 60%, 70%, 80%, 90%, 95%, 97%, 98%, 99% acetone soln (300ml) and was dewatered respectively two days, counts 18 days; Dehydration is seven days in 100% acetone soln.
(3) preparation plasticized silica gel solution: get dimethyl silicone polymer 0.5kg; Chloroplatinic acid 0.0025kg, composite organic modified silane 0.01kg, ether 0.025kg mixes, and takes out 300ml, and the kidney after the dehydration is put into, and under the normal temperature vacuum, carries out vacuum infiltration 10 days.
(4) obtain the organoalkoxysilane of 30ml, join mixing in the silica gel solution, unsettled the shelving of kidney that vacuum infiltration is crossed is to carry out the air-set moulding under the condition of 60-70% in normal temperature, humidity.
Formalin of being got in the above-mentioned enforcement and biological plasticized silica gel solution, the preservation biological sample that their capacity is made for the energy submergence is as the criterion.The time of fixing, dehydration, vacuum infiltration with the volume size of biological sample itself and biological sample internal organizational structure thick, approach, loosen, tighten, dredge, close and decide.Generally to upper and lower two days of the time of human body specimen plasticizing process (promptly ± 2 day).
Claims (2)
1, a kind of new technology that is used for biological preservation, divide (1), fixing, (2), dehydration, (3), vacuum infiltration, (4), hardened forming technology, wherein (1), technique for fixing is that biological sample was put into 5% formalin solution fixedly 10-20 days at normal temperatures, feature of the present invention is (2), dewatering process is that the biological sample after will fixing at normal temperatures is placed on and carries out the classification dehydration in the acetone soln of different content, promptly 50%, 60%, 70%, 80%, 90%, 95%, 97%, 98%, respectively dewatered in 99% the acetone soln two days, dehydration is seven days in 100% acetone soln; (3), vacuum impregnation technique is with the biological sample after the dehydration at normal temperatures, drop in the biological plasticized silica gel solution, adopt the discontinuous negative pressure of vacuum infiltration method of bleeding, silica gel is fully infiltrated, time 10-25 days, this silica gel composition was made up of dimethyl silicone polymer, chloroplatinic acid, composite organic modified silane, ether; (4) hardened forming technology, under normal temperature, the condition of humidity at 60-70%, unsettled shelving is with organoalkoxysilane air-set moulding with the biological sample after fixing, dehydration, the infiltration.
2. a kind of new technology that biological sample is preserved that is used for according to claim 1, it is characterized in that each the composition weight of silica gel in the vacuum impregnation technique is: every 100kg dimethyl silicone polymer, get chloroplatinic acid 1-5kg, composite organic modified silane 2-8kg, ether 5-10kg, the organoalkoxysilane in (4) hardened forming technology is got 0-2kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96116981A CN1055373C (en) | 1996-06-27 | 1996-06-27 | Organism preserving technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96116981A CN1055373C (en) | 1996-06-27 | 1996-06-27 | Organism preserving technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1145717A CN1145717A (en) | 1997-03-26 |
| CN1055373C true CN1055373C (en) | 2000-08-16 |
Family
ID=5123927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96116981A Expired - Fee Related CN1055373C (en) | 1996-06-27 | 1996-06-27 | Organism preserving technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1055373C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397987C (en) * | 2006-04-17 | 2008-07-02 | 南京医科大学 | A kind of preparation method of human anatomy plasticized specimen |
| CN102125025B (en) * | 2010-12-17 | 2013-01-09 | 滨州医学院 | Method for plasticizing biological specimen |
| CN104210311B (en) * | 2014-04-10 | 2017-03-29 | 河南科技大学 | A kind of preparation method of three-dimensional dry flower |
| CN110150263A (en) * | 2019-06-26 | 2019-08-23 | 广东药科大学 | A plastination preservation method for unearthed ancient corpses or skeletons |
| CN111213632B (en) * | 2019-11-19 | 2022-03-01 | 长春中医药大学 | Preparation method of animal medicine epoxy resin specimen |
| CN111449059A (en) * | 2020-05-12 | 2020-07-28 | 西南大学 | A solidified specimen of citrus orange fruit |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4205059A (en) * | 1977-03-09 | 1980-05-27 | Hagens Gunther Von | Animal and vegetal tissues permanently preserved by synthetic resin impregnation |
| JPH0374301A (en) * | 1989-08-16 | 1991-03-28 | Akio Imuro | Production of large-sized resin-embedded biological specimen |
| WO1995022894A1 (en) * | 1994-02-28 | 1995-08-31 | Michigan State University | Method for preservation of biological tissue |
-
1996
- 1996-06-27 CN CN96116981A patent/CN1055373C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4205059A (en) * | 1977-03-09 | 1980-05-27 | Hagens Gunther Von | Animal and vegetal tissues permanently preserved by synthetic resin impregnation |
| JPH0374301A (en) * | 1989-08-16 | 1991-03-28 | Akio Imuro | Production of large-sized resin-embedded biological specimen |
| WO1995022894A1 (en) * | 1994-02-28 | 1995-08-31 | Michigan State University | Method for preservation of biological tissue |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1145717A (en) | 1997-03-26 |
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