JP2506396B2 - ジオキセタン類の酵素的に誘導される分解を使用する物質検出法 - Google Patents
ジオキセタン類の酵素的に誘導される分解を使用する物質検出法Info
- Publication number
- JP2506396B2 JP2506396B2 JP62502145A JP50214587A JP2506396B2 JP 2506396 B2 JP2506396 B2 JP 2506396B2 JP 62502145 A JP62502145 A JP 62502145A JP 50214587 A JP50214587 A JP 50214587A JP 2506396 B2 JP2506396 B2 JP 2506396B2
- Authority
- JP
- Japan
- Prior art keywords
- group
- enzyme
- ring
- dioxetane
- carbon atoms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D321/00—Heterocyclic compounds containing rings having two oxygen atoms as the only ring hetero atoms, not provided for by groups C07D317/00 - C07D319/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/12—Esters of phosphoric acids with hydroxyaryl compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
- C07F9/6551—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a four-membered ring
- C07F9/65512—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a four-membered ring condensed with carbocyclic rings or carbocyclic ring systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6564—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having phosphorus atoms, with or without nitrogen, oxygen, sulfur, selenium or tellurium atoms, as ring hetero atoms
- C07F9/6571—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having phosphorus atoms, with or without nitrogen, oxygen, sulfur, selenium or tellurium atoms, as ring hetero atoms having phosphorus and oxygen atoms as the only ring hetero atoms
- C07F9/6574—Esters of oxyacids of phosphorus
- C07F9/65742—Esters of oxyacids of phosphorus non-condensed with carbocyclic rings or heterocyclic rings or ring systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/26—Acyclic or carbocyclic radicals, substituted by hetero rings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US88982386A | 1986-07-24 | 1986-07-24 | |
US889,823 | 1986-07-24 | ||
CA000604507A CA1340590C (en) | 1986-07-24 | 1989-06-30 | Chemiluminescence enhancement |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH04500901A JPH04500901A (ja) | 1992-02-20 |
JP2506396B2 true JP2506396B2 (ja) | 1996-06-12 |
Family
ID=33565664
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62502145A Expired - Lifetime JP2506396B2 (ja) | 1986-07-24 | 1987-03-11 | ジオキセタン類の酵素的に誘導される分解を使用する物質検出法 |
Country Status (6)
Families Citing this family (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5004565A (en) * | 1986-07-17 | 1991-04-02 | The Board Of Governors Of Wayne State University | Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes |
WO1988000695A1 (en) * | 1986-07-24 | 1988-01-28 | Quest Systems, Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
US4931223A (en) * | 1986-07-24 | 1990-06-05 | Tropix, Inc. | Methods of using chemiluminescent 1,2-dioxetanes |
US4978614A (en) * | 1988-10-26 | 1990-12-18 | Tropix, Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
USRE36536E (en) * | 1986-07-24 | 2000-01-25 | Tropix. Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
US5109124A (en) * | 1988-06-01 | 1992-04-28 | Biogen, Inc. | Nucleic acid probe linked to a label having a terminal cysteine |
US5094939A (en) * | 1988-07-19 | 1992-03-10 | Fujirebio, Inc. | Chemiluminescence assays using stabilized dioxetane derivatives |
US5132204A (en) * | 1989-05-31 | 1992-07-21 | Chiron Corporation | Chemiluminescent double-triggered 1, 2-dioxetanes |
GB2233451B (en) * | 1989-06-27 | 1993-09-15 | Tropix Inc | Chemiluminescence enhancement |
US6022964A (en) | 1989-07-17 | 2000-02-08 | Tropix, Inc. | Chemiluminescent 1,2-dioxetanes |
US5362916A (en) * | 1989-09-06 | 1994-11-08 | Tropix, Inc. | Synthesis of mercaptaryl or hydroxyaryl enol ether alkali metal salts |
EP0441948A4 (en) * | 1989-09-06 | 1992-06-03 | Tropix, Inc. | Synthesis of stable, water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
US5225584A (en) * | 1989-09-06 | 1993-07-06 | Tropix, Inc. | Synthesis of stable water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
JP2979414B2 (ja) * | 1989-09-29 | 1999-11-15 | 富士レビオ株式会社 | 磁性粒子およびそれを用いた免疫測定法 |
JPH03225277A (ja) * | 1990-01-31 | 1991-10-04 | Fujirebio Inc | 多項目の免疫化学的測定法 |
US5578498A (en) | 1991-05-22 | 1996-11-26 | Behringwerke Ag | Metal chelate containing compositions for use in chemiluminescent assays |
US5340716A (en) * | 1991-06-20 | 1994-08-23 | Snytex (U.S.A.) Inc. | Assay method utilizing photoactivated chemiluminescent label |
US6251581B1 (en) | 1991-05-22 | 2001-06-26 | Dade Behring Marburg Gmbh | Assay method utilizing induced luminescence |
IL102523A0 (en) * | 1991-07-31 | 1993-01-14 | Du Pont | Enhancing the chemiluminescence of enzyme-triggered 1,2-dioxetanes |
US5393469A (en) * | 1992-03-20 | 1995-02-28 | Lumigen, Inc. | Polymeric phosphonium salts providing enhanced chemiluminescence from 1,2-dioxetanes |
WO1994002486A1 (en) * | 1992-07-20 | 1994-02-03 | Syntex (U.S.A.) Inc. | Novel chemiluminescent compounds and methods of use |
ATE162892T1 (de) * | 1992-07-31 | 1998-02-15 | Behringwerke Ag | Photoaktivierbare chemiluminizierende matrices |
DE4304728C2 (de) * | 1993-02-13 | 1997-04-10 | Igor Dr Popov | Verfahren und Testbesteck zur Bestimmung von Ascorbinsäure in biologischen Proben |
EP0739163A1 (en) * | 1994-01-10 | 1996-10-30 | Hemasure, Inc. | Inactivation of viruses present in blood components using chemically-activated compounds |
US5589328A (en) * | 1994-08-04 | 1996-12-31 | Mahant; Vijay K. | Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates |
US5773628A (en) * | 1994-11-14 | 1998-06-30 | Tropix, Inc. | 1,2-dioxetane compounds with haloalkoxy groups, methods preparation and use |
US5578253A (en) | 1994-11-23 | 1996-11-26 | Lumigen, Inc. | Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use |
US5663054A (en) | 1995-03-03 | 1997-09-02 | Abbott Laboratories | Determination of steroids by competitive immunoassay |
US6245928B1 (en) * | 1995-07-31 | 2001-06-12 | Lumigen, Inc. | Water soluble tri-substituted 1,2-dioxetane compounds having increased storage stability, synthetic processes and intermediates |
US5777135A (en) * | 1995-07-31 | 1998-07-07 | Lumigen, Inc. | Di-substituted 1,2-dioxetane compounds having increased water solubility and assay compositions |
US5721370A (en) * | 1995-07-31 | 1998-02-24 | Lumigen Inc. | Water soluble tri-substituted 1,2-dioxetane compounds and assay compositions having increased storage stability |
US5631167A (en) * | 1995-07-31 | 1997-05-20 | Bayer Corporation | Capsule chemistry analytical methods employing dioxetane chemiluminescence |
DE19536166C1 (de) | 1995-09-29 | 1997-03-06 | Siegfried Dr Krell | Verfahren zur Bestimmung von Antikörpern gegen Treponema pallidum (Syphilis) |
JP3182527B2 (ja) * | 1996-09-03 | 2001-07-03 | 株式会社ヤトロン | 化学発光の測定方法及びキット |
US6165800A (en) * | 1997-05-30 | 2000-12-26 | Bayer Corporation | Chemiluminescent energy transfer conjugates and their uses as labels in binding assays |
CA2370520A1 (en) * | 1999-04-16 | 2000-10-26 | Komandoor Elayavalli Achyuthan | Viral detection method using viral encoded enzymes and chemiluminescent substrates |
AU3958999A (en) * | 1999-06-01 | 2000-12-18 | Kyokuto Pharmaceutical Industrial Co., Ltd. | Method for assaying anti-gad antibody and kit |
US7432063B2 (en) | 2002-02-14 | 2008-10-07 | Kalobios Pharmaceuticals, Inc. | Methods for affinity maturation |
US7390670B2 (en) | 2003-02-20 | 2008-06-24 | Lumigen, Inc. | Signalling compounds and methods for detecting hydrogen peroxide |
US20050019955A1 (en) * | 2003-07-23 | 2005-01-27 | Dahl Jeremy E. | Luminescent heterodiamondoids as biological labels |
US20090181359A1 (en) | 2007-10-25 | 2009-07-16 | Lou Sheng C | Method of performing ultra-sensitive immunoassays |
US8222048B2 (en) | 2007-11-05 | 2012-07-17 | Abbott Laboratories | Automated analyzer for clinical laboratory |
US8518658B1 (en) | 2009-04-27 | 2013-08-27 | University Of South Florida | ATP-bioluminescence immunoassay |
US8784734B2 (en) | 2010-05-20 | 2014-07-22 | Abbott Laboratories | Reusable sheaths for separating magnetic particles |
Family Cites Families (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2478377A (en) | 1945-06-29 | 1949-08-09 | Eastman Kodak Co | Preparation of esters of acids of phosphorus |
US3009962A (en) | 1958-08-21 | 1961-11-21 | Research Corp | Organic peroxides |
NL152290B (nl) | 1965-09-08 | 1977-02-15 | American Cyanamid Co | Werkwijze ter bereiding van chemiluminescerende mengsels. |
US3399137A (en) | 1965-09-08 | 1968-08-27 | American Cyanamid Co | Generation of light by the reaction of anhydrides of oxalic acid with a peroxide in tpresence of a fluorescer |
US3442814A (en) | 1965-09-23 | 1969-05-06 | American Cyanamid Co | Generation of chemiluminescent light |
DE1670497C3 (de) * | 1967-06-06 | 1973-12-20 | Chinoin Gyogyszer- Es Vegyeszeti Termekek Gyara Rt, Budapest | Isochinohnderivate und Verfahren zu deren Herstellung |
US3442813A (en) | 1967-09-13 | 1969-05-06 | American Cyanamid Co | Generation of light by the reaction of oxalyl chloride pyridine and tertiary amine complexes with peroxides in the presence of a fluorescer |
US3677957A (en) | 1969-12-17 | 1972-07-18 | American Cyanamid Co | Chemiluminescent reaction of chlorinated ethylene carbonate with hydrogen peroxide in the presence of a fluorescer |
US3720622A (en) | 1970-01-02 | 1973-03-13 | American Cyanamid Co | Generation of light from the decomposition of dioxetaneones in the presence of a fluorescer |
US3763188A (en) | 1972-01-19 | 1973-10-02 | Du Pont | Spiro(1,1-polyethylenedi(oxymethyl))-3-oxetanes |
US3862142A (en) | 1972-06-13 | 1975-01-21 | Research Corp | Method of reductive ozonolysis of olefins to produce carbonyl compounds and intermediate products |
US4604364A (en) | 1974-01-04 | 1986-08-05 | Kosak Kenneth M | Bioluminescent tracer composition and method of use in immunoassays |
CA1044639A (en) | 1974-06-12 | 1978-12-19 | Research Corporation | Polymer-bound photosensitizing catalysts and photosensitized reactions utilizing same |
US4315998A (en) | 1974-06-12 | 1982-02-16 | Research Corporation | Polymer-bound photosensitizing catalysts |
FR2383404A1 (fr) | 1977-03-11 | 1978-10-06 | Anvar | Procede pour le captage, la recuperation et eventuellement le stockage de l'energie lumineuse |
IL51668A (en) | 1977-03-16 | 1981-12-31 | Israel State | Analytical method for the quantitative determination of immunogens and antibodies and a kit therefor |
US4372745A (en) | 1979-12-19 | 1983-02-08 | Electro-Nucleonics, Inc. | Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer |
JPS5742686A (en) | 1980-08-28 | 1982-03-10 | Asahi Chem Ind Co Ltd | Preparation of dioxetane |
US4663278A (en) | 1982-04-30 | 1987-05-05 | Syva Company | Agglutination dependent enzyme channeling immunoassay |
US4446233A (en) | 1982-05-05 | 1984-05-01 | E. I. Du Pont De Nemours And Company | Homogeneous immunoassay using covalent hybrid antibodies |
JPS59500735A (ja) | 1983-04-18 | 1984-04-26 | エス・ア−ル・アイ・インタ−ナシヨナル | 人間の癌診断のための方法および試験キツト |
US5707559A (en) * | 1986-07-17 | 1998-01-13 | Tropix, Inc. | Chemiluminescent 1,2-dioxetane compounds |
US4962192A (en) * | 1986-07-17 | 1990-10-09 | Board Of Governors Of Wayne State University | Chemiluminescent 1,2-dioxetane compounds |
US4857652A (en) * | 1986-07-17 | 1989-08-15 | Board Of Governors Of Wayne State University | Chemiluminescent 1,2-dioxetane compounds |
WO1988000695A1 (en) | 1986-07-24 | 1988-01-28 | Quest Systems, Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
USRE36536E (en) * | 1986-07-24 | 2000-01-25 | Tropix. Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
US5605795A (en) * | 1986-07-24 | 1997-02-25 | Tropix, Inc. | Assays using chemiluminescent, enzymatically cleavable substituted 1,2-dioxetanes and kits therefor |
US4956477A (en) * | 1987-12-31 | 1990-09-11 | Tropix, Inc. | Synthesis of 1,2-dioxetanes |
US5639907A (en) * | 1986-07-24 | 1997-06-17 | Tropix, Inc. | Synthesis of 1,2-dioxetanes and intermediates therefor |
US5177241A (en) * | 1987-12-31 | 1993-01-05 | Bronstein Irena Y | Synthesis of 1,2-dioxetanes and intermediates therefor |
US4978614A (en) * | 1988-10-26 | 1990-12-18 | Tropix, Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
US5856522A (en) * | 1995-05-04 | 1999-01-05 | Tropix, Inc. | Method of using synthesis of 1,2-dioxetanes and kits therefore |
US5625077A (en) * | 1987-12-31 | 1997-04-29 | Tropix, Inc. | 1,2-dioxetanes |
US5538847A (en) * | 1989-07-17 | 1996-07-23 | Tropix, Inc. | Chemiluminescent 1,2-dioxetanes |
US5679802A (en) * | 1989-07-17 | 1997-10-21 | Tropix, Inc. | 1,2-dioxetanes useful in chemiluminescent immunoassays |
US5330900A (en) * | 1987-12-31 | 1994-07-19 | Tropix, Inc. | Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes |
US5582980A (en) * | 1989-07-17 | 1996-12-10 | Tropix, Inc. | Chemiluminescent 1,2-dioxetanes |
US5756770A (en) * | 1987-12-31 | 1998-05-26 | Tropix, Inc. | Synthesis of stable, water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
US5362916A (en) * | 1989-09-06 | 1994-11-08 | Tropix, Inc. | Synthesis of mercaptaryl or hydroxyaryl enol ether alkali metal salts |
US5225584A (en) * | 1989-09-06 | 1993-07-06 | Tropix, Inc. | Synthesis of stable water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
US5547836A (en) * | 1990-08-30 | 1996-08-20 | Tropix, Inc. | Enhancement of chemiluminescent assays |
US5336596A (en) * | 1991-12-23 | 1994-08-09 | Tropix, Inc. | Membrane for chemiluminescent blotting applications |
AU1518195A (en) | 1993-12-23 | 1995-07-10 | Tropix, Inc. | Chemiluminescent energy transfer assays |
US5679803A (en) * | 1995-10-25 | 1997-10-21 | Tropix, Inc. | 1,2 chemiluminescent dioxetanes of improved performance |
AU708266B2 (en) * | 1995-10-17 | 1999-07-29 | Applied Biosystems, Llc | 1,2 chemiluminescent dioxetanes of improved performance |
-
1987
- 1987-03-11 WO PCT/US1987/000554 patent/WO1988000695A1/en active IP Right Grant
- 1987-03-11 EP EP87902259A patent/EP0275260B1/en not_active Expired - Lifetime
- 1987-03-11 BR BR8707399A patent/BR8707399A/pt unknown
- 1987-03-11 JP JP62502145A patent/JP2506396B2/ja not_active Expired - Lifetime
- 1987-03-11 DE DE87902259T patent/DE3788203T2/de not_active Expired - Lifetime
-
1996
- 1996-12-16 US US08/767,282 patent/US5866389A/en not_active Expired - Lifetime
-
1997
- 1997-08-14 US US08/911,271 patent/US5869705A/en not_active Expired - Lifetime
-
1998
- 1998-10-30 US US09/182,416 patent/US6113816A/en not_active Expired - Lifetime
-
2000
- 2000-03-29 US US09/537,693 patent/US6322727B1/en not_active Expired - Lifetime
-
2001
- 2001-09-25 US US09/960,932 patent/US6514717B2/en not_active Expired - Lifetime
-
2002
- 2002-12-20 US US10/323,667 patent/US6913897B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US6514717B2 (en) | 2003-02-04 |
BR8707399A (pt) | 1988-09-13 |
US6913897B2 (en) | 2005-07-05 |
JPH04500901A (ja) | 1992-02-20 |
US20030175838A1 (en) | 2003-09-18 |
US5869705A (en) | 1999-02-09 |
EP0275260A4 (en) | 1990-01-08 |
US5866389A (en) | 1999-02-02 |
EP0275260A1 (en) | 1988-07-27 |
EP0275260B1 (en) | 1993-11-18 |
DE3788203T2 (de) | 1994-03-31 |
US6113816A (en) | 2000-09-05 |
US20020102624A1 (en) | 2002-08-01 |
WO1988000695A1 (en) | 1988-01-28 |
US6322727B1 (en) | 2001-11-27 |
DE3788203D1 (de) | 1993-12-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2506396B2 (ja) | ジオキセタン類の酵素的に誘導される分解を使用する物質検出法 | |
US4978614A (en) | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes | |
US5112960A (en) | Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes | |
JP2922237B2 (ja) | 化学発光性1,2―ジオキセタン類の改良使用法 | |
US5543295A (en) | Chemiluminescent 3--(Substituted adamant--2'--Ylidene) 1,2--Dioxetanes | |
US5220005A (en) | Substituted adamantyl dioxetanes | |
US5849495A (en) | Chemiluminescent energy transfer assays | |
JP4069987B2 (ja) | ハロアルコキシ基を有する新規な1,2−ジオキセタン化合物、その調製方法及びその用途 | |
US20040171098A1 (en) | Signalling compounds for use in methods of detecting hydrogen peroxide | |
JP4074335B2 (ja) | 電子数の多い化学発光性アリール置換1,2−ジオキセタン | |
US5089630A (en) | Dioxetanes for use in assays | |
US5326882A (en) | Chemiluminescent 3-(substituted Adamant-2'-Ylidene) 1,2-dioxetanes | |
JPH0670797A (ja) | 化学発光反応を利用する酵素の測定法 | |
US5605795A (en) | Assays using chemiluminescent, enzymatically cleavable substituted 1,2-dioxetanes and kits therefor | |
JPH05503714A (ja) | 化学発光性3―(置換アダマント―2′―イリデン)1,2―ジオキセタン | |
JP2583327B2 (ja) | 分析で使用するジオキセタン | |
US5831102A (en) | Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes | |
USRE36536E (en) | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes | |
JP2999810B2 (ja) | 1,2−ジオキセタン化合物 | |
CA1341031C (en) | Method of detecting a substance using enzymatically-induced decomposition of 1,2-dioxetanes | |
JPH11500222A (ja) | 化学ルミネセンスエネルギー移動アッセイ | |
CA2033331C (en) | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes | |
JPH1121285A (ja) | 化学発光性1,2−ジオキセタン化合物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
R371 | Transfer withdrawn |
Free format text: JAPANESE INTERMEDIATE CODE: R371 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S201 | Request for registration of exclusive licence |
Free format text: JAPANESE INTERMEDIATE CODE: R314201 |
|
R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
R371 | Transfer withdrawn |
Free format text: JAPANESE INTERMEDIATE CODE: R371 |
|
S201 | Request for registration of exclusive licence |
Free format text: JAPANESE INTERMEDIATE CODE: R314201 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
EXPY | Cancellation because of completion of term |