JP2024006086A - Ｌ－ピログルタミン酸開環酵素 - Google Patents
Ｌ－ピログルタミン酸開環酵素 Download PDFInfo
- Publication number
- JP2024006086A JP2024006086A JP2022106652A JP2022106652A JP2024006086A JP 2024006086 A JP2024006086 A JP 2024006086A JP 2022106652 A JP2022106652 A JP 2022106652A JP 2022106652 A JP2022106652 A JP 2022106652A JP 2024006086 A JP2024006086 A JP 2024006086A
- Authority
- JP
- Japan
- Prior art keywords
- pyroglutamic acid
- opening
- ring
- enzyme
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical group OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 title claims abstract description 125
- ODHCTXKNWHHXJC-UHFFFAOYSA-N acide pyroglutamique Natural products OC(=O)C1CCC(=O)N1 ODHCTXKNWHHXJC-UHFFFAOYSA-N 0.000 title claims abstract description 125
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 116
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 116
- 238000007142 ring opening reaction Methods 0.000 title claims abstract description 98
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 150000001413 amino acids Chemical class 0.000 claims abstract description 13
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 82
- 230000000694 effects Effects 0.000 claims description 49
- 229960002989 glutamic acid Drugs 0.000 claims description 39
- 235000013305 food Nutrition 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 20
- 102000005367 Carboxypeptidases Human genes 0.000 claims description 17
- 108010006303 Carboxypeptidases Proteins 0.000 claims description 17
- 230000001965 increasing effect Effects 0.000 claims description 14
- 239000011701 zinc Substances 0.000 claims description 14
- 235000013555 soy sauce Nutrition 0.000 claims description 13
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 12
- 229910052725 zinc Inorganic materials 0.000 claims description 12
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 11
- 240000003768 Solanum lycopersicum Species 0.000 claims description 11
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 10
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 10
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 10
- ODHCTXKNWHHXJC-GSVOUGTGSA-N Pyroglutamic acid Natural products OC(=O)[C@H]1CCC(=O)N1 ODHCTXKNWHHXJC-GSVOUGTGSA-N 0.000 claims description 9
- 235000013922 glutamic acid Nutrition 0.000 claims description 9
- 239000004220 glutamic acid Substances 0.000 claims description 9
- 235000001014 amino acid Nutrition 0.000 claims description 8
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 7
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 claims description 7
- 229940024606 amino acid Drugs 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 101710089972 Ochratoxinase Proteins 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 abstract description 29
- 239000004278 EU approved seasoning Substances 0.000 abstract description 9
- 235000011194 food seasoning agent Nutrition 0.000 abstract description 9
- 235000013324 preserved food Nutrition 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 description 30
- 239000000872 buffer Substances 0.000 description 24
- 239000000243 solution Substances 0.000 description 24
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 21
- 235000018102 proteins Nutrition 0.000 description 18
- 102000004169 proteins and genes Human genes 0.000 description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 16
- 238000004458 analytical method Methods 0.000 description 10
- 239000013078 crystal Substances 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 229920002643 polyglutamic acid Polymers 0.000 description 9
- 238000000746 purification Methods 0.000 description 9
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 8
- 108020004414 DNA Proteins 0.000 description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 8
- 238000002425 crystallisation Methods 0.000 description 7
- 230000008025 crystallization Effects 0.000 description 7
- 238000010828 elution Methods 0.000 description 7
- 238000006911 enzymatic reaction Methods 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 238000003860 storage Methods 0.000 description 7
- 102100025908 5-oxoprolinase Human genes 0.000 description 6
- 108010076504 Protein Sorting Signals Proteins 0.000 description 6
- 244000269722 Thea sinensis Species 0.000 description 6
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 108010092650 Pyroglutamate Hydrolase Proteins 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- 238000001641 gel filtration chromatography Methods 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 235000013616 tea Nutrition 0.000 description 5
- 235000019583 umami taste Nutrition 0.000 description 5
- 239000013613 expression plasmid Substances 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- CSRCBLMBBOJYEX-UHFFFAOYSA-M sodium;2-morpholin-4-ylethanesulfonic acid;hydroxide Chemical compound [OH-].[Na+].OS(=O)(=O)CCN1CCOCC1 CSRCBLMBBOJYEX-UHFFFAOYSA-M 0.000 description 4
- 241000672609 Escherichia coli BL21 Species 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 238000001261 affinity purification Methods 0.000 description 3
- 235000013334 alcoholic beverage Nutrition 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 3
- 238000005349 anion exchange Methods 0.000 description 3
- 238000002869 basic local alignment search tool Methods 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 235000019985 fermented beverage Nutrition 0.000 description 3
- 235000021107 fermented food Nutrition 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 108700022290 poly(gamma-glutamic acid) Proteins 0.000 description 3
- 235000019833 protease Nutrition 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 235000015067 sauces Nutrition 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 230000009967 tasteless effect Effects 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
- 229930182816 L-glutamine Natural products 0.000 description 2
- 235000015429 Mirabilis expansa Nutrition 0.000 description 2
- 244000294411 Mirabilis expansa Species 0.000 description 2
- YBHQCJILTOVLHD-YVMONPNESA-N Mirin Chemical compound S1C(N)=NC(=O)\C1=C\C1=CC=C(O)C=C1 YBHQCJILTOVLHD-YVMONPNESA-N 0.000 description 2
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- 241000179039 Paenibacillus Species 0.000 description 2
- 102000018120 Recombinases Human genes 0.000 description 2
- 108010091086 Recombinases Proteins 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 241000186547 Sporosarcina Species 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
- 238000002447 crystallographic data Methods 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 235000019688 fish Nutrition 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 235000008960 ketchup Nutrition 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000013536 miso Nutrition 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 238000007363 ring formation reaction Methods 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 235000015113 tomato pastes and purées Nutrition 0.000 description 2
- 101710175623 5-oxoprolinase Proteins 0.000 description 1
- 241000588813 Alcaligenes faecalis Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010073324 Glutaminase Proteins 0.000 description 1
- 102000009127 Glutaminase Human genes 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 description 1
- 108010020346 Polyglutamic Acid Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 101710149909 Zinc carboxypeptidase Proteins 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 1
- 229940005347 alcaligenes faecalis Drugs 0.000 description 1
- 238000003277 amino acid sequence analysis Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 235000020279 black tea Nutrition 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000003028 enzyme activity measurement method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- -1 fermented seasonings Chemical compound 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 229940043131 pyroglutamate Drugs 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 235000019992 sake Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 235000020083 shōchū Nutrition 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- ZNJHFNUEQDVFCJ-UHFFFAOYSA-M sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid;hydroxide Chemical compound [OH-].[Na+].OCCN1CCN(CCS(O)(=O)=O)CC1 ZNJHFNUEQDVFCJ-UHFFFAOYSA-M 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000012609 strong anion exchange resin Substances 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 235000015041 whisky Nutrition 0.000 description 1
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022106652A JP2024006086A (ja) | 2022-06-30 | 2022-06-30 | Ｌ－ピログルタミン酸開環酵素 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022106652A JP2024006086A (ja) | 2022-06-30 | 2022-06-30 | Ｌ－ピログルタミン酸開環酵素 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2024006086A true JP2024006086A (ja) | 2024-01-17 |
| JP2024006086A5 JP2024006086A5 (enExample) | 2025-04-14 |
Family
ID=89539615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022106652A Pending JP2024006086A (ja) | 2022-06-30 | 2022-06-30 | Ｌ－ピログルタミン酸開環酵素 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2024006086A (enExample) |
-
2022
- 2022-06-30 JP JP2022106652A patent/JP2024006086A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Namwong et al. | A halophilic serine proteinase from Halobacillus sp. SR5-3 isolated from fish sauce: purification and characterization | |
| KR102132381B1 (ko) | 아스로박터 글로비포미스에 의해 생산되는 케토오스 3-에피머라제 | |
| KR20030078910A (ko) | 아가레이즈 및 그의 유전자 | |
| CN102203248B (zh) | 鞣酸酶、编码其的基因及其制造法 | |
| JP5006380B2 (ja) | 新規なα−ガラクトシダーゼ | |
| KR101091138B1 (ko) | 류코노스톡 락티스로부터 유래된 글루칸수크라제 및 그 제조방법 | |
| CN101918551B (zh) | 草酸脱羧酶的生产中利用的重组表达质粒载体及重组菌、以及重组草酸脱羧酶的生产方法 | |
| CN112852788A (zh) | 一种碱性底物选择性提高的枯草蛋白酶e突变体及其应用 | |
| JP7138867B2 (ja) | カルバミン酸エチルの分解 | |
| KR101979213B1 (ko) | 변형된 당 대사 경로를 갖는 재조합 균주 및 이를 이용한 당이성화 효소의 스크리닝 방법 | |
| CN109563504B (zh) | 核苷酶 | |
| JP2024006086A (ja) | Ｌ－ピログルタミン酸開環酵素 | |
| JP2003250588A (ja) | タンナーゼ、その遺伝子及びタンナーゼの製造法 | |
| KR102004945B1 (ko) | 아밀로수크라제의 발현 시스템 및 이를 이용한 투라노스의 생산 | |
| JP5283154B2 (ja) | トリプシン様酵素 | |
| US5190875A (en) | Peptide amidase and the use thereof | |
| KR101796060B1 (ko) | 한천을 분해하여 네오한천올리고당을 생성할 수 있는 아가라제 | |
| KR101778878B1 (ko) | 박테로이데스 속 미생물 유래의 ｇａｂａ 생성 고활성 글루탐산탈탄산효소 및 이의 용도 | |
| JP6656919B2 (ja) | ５−オキソプロリナーゼ、５−オキソプロリナーゼ遺伝子、および５−オキソプロリナーゼの製造方法 | |
| EP2084283A1 (en) | Mutant dna polymerases and their genes | |
| CN113073107B (zh) | 一种甘露聚糖酶基因AbMan5、重组表达质粒、重组表达菌株、甘露聚糖酶及其应用 | |
| JP2007189905A (ja) | 好アルカリ性サイクロデキストラン合成酵素遺伝子を含有するｄｎａ、組み換え体ｄｎａ、および好アルカリ性サイクロデキストラン合成酵素の製造法 | |
| CN112055751B (zh) | 修饰型酯酶和其用途 | |
| KR101796059B1 (ko) | 한천을 가수분해하여 사탄당을 생성하는 능력이 우수한 아가라아제 및 이를 코드하는 유전자 | |
| WO1997027313A1 (en) | Cold-active protease cp70 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20250404 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20250404 |