JP2023076815A - 蛍光顕微鏡システムおよび方法 - Google Patents

蛍光顕微鏡システムおよび方法 Download PDF

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Publication number
JP2023076815A
JP2023076815A JP2022186269A JP2022186269A JP2023076815A JP 2023076815 A JP2023076815 A JP 2023076815A JP 2022186269 A JP2022186269 A JP 2022186269A JP 2022186269 A JP2022186269 A JP 2022186269A JP 2023076815 A JP2023076815 A JP 2023076815A
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Japan
Prior art keywords
imaging mode
sample
control unit
fluorophores
different
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JP2022186269A
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English (en)
Japanese (ja)
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JP2023076815A5 (enExample
Inventor
リッチェル カイ
Ritschel Kai
ダイスラー ベンヤミン
Deissler Benjamin
イェーネルト デニス
Jaehnert Dennis
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Leica Microsystems CMS GmbH
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Leica Microsystems CMS GmbH
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Application filed by Leica Microsystems CMS GmbH filed Critical Leica Microsystems CMS GmbH
Publication of JP2023076815A publication Critical patent/JP2023076815A/ja
Publication of JP2023076815A5 publication Critical patent/JP2023076815A5/ja
Pending legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
JP2022186269A 2021-11-23 2022-11-22 蛍光顕微鏡システムおよび方法 Pending JP2023076815A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21209766 2021-11-23
EP21209766.1A EP4050396B1 (en) 2021-11-23 2021-11-23 Fluorescence microscope system and method

Publications (2)

Publication Number Publication Date
JP2023076815A true JP2023076815A (ja) 2023-06-02
JP2023076815A5 JP2023076815A5 (enExample) 2025-12-02

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ID=78770400

Family Applications (1)

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JP2022186269A Pending JP2023076815A (ja) 2021-11-23 2022-11-22 蛍光顕微鏡システムおよび方法

Country Status (4)

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US (1) US12436373B2 (enExample)
EP (1) EP4050396B1 (enExample)
JP (1) JP2023076815A (enExample)
CN (1) CN116149038A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4092466A1 (en) * 2021-05-21 2022-11-23 Leica Microsystems CMS GmbH Method for obtaining an operating value of an illumination parameter for examining a fluorescent sample, method for examining a fluorescent sample, microscope system and computer program

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004013128A (ja) * 2002-06-11 2004-01-15 Olympus Corp 蛍光顕微鏡
JP2005331887A (ja) * 2004-05-21 2005-12-02 Keyence Corp 蛍光顕微鏡、蛍光顕微鏡装置を使用した表示方法、蛍光顕微鏡画像表示プログラム及びコンピュータで読み取り可能な記録媒体並びに記憶した機器
JP2007199605A (ja) * 2006-01-30 2007-08-09 Osaka Prefecture Univ 蛍光顕微鏡
JP2011252950A (ja) * 2010-05-31 2011-12-15 Nikon Corp 撮像装置および撮像プログラム
JP2013020204A (ja) * 2011-07-14 2013-01-31 Olympus Corp 蛍光顕微鏡装置
JP2020052434A (ja) * 2016-03-10 2020-04-02 シスメックス株式会社 光学機器および像の形成方法
US11487097B1 (en) * 2020-01-26 2022-11-01 Ramona Optics Inc. System and method for synchronized fluorescence capture

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10222359B4 (de) 2002-05-21 2005-01-05 Max Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur spektral differenzierenden, bildgebenden Messung von Fluoreszenzlicht
GB0707433D0 (en) * 2007-04-18 2007-05-23 Stfc Science & Technology Fluorescence measurement
US10768105B1 (en) * 2016-07-29 2020-09-08 Labrador Diagnostics Llc Image analysis and measurement of biological samples

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004013128A (ja) * 2002-06-11 2004-01-15 Olympus Corp 蛍光顕微鏡
JP2005331887A (ja) * 2004-05-21 2005-12-02 Keyence Corp 蛍光顕微鏡、蛍光顕微鏡装置を使用した表示方法、蛍光顕微鏡画像表示プログラム及びコンピュータで読み取り可能な記録媒体並びに記憶した機器
JP2007199605A (ja) * 2006-01-30 2007-08-09 Osaka Prefecture Univ 蛍光顕微鏡
JP2011252950A (ja) * 2010-05-31 2011-12-15 Nikon Corp 撮像装置および撮像プログラム
JP2013020204A (ja) * 2011-07-14 2013-01-31 Olympus Corp 蛍光顕微鏡装置
JP2020052434A (ja) * 2016-03-10 2020-04-02 シスメックス株式会社 光学機器および像の形成方法
US11487097B1 (en) * 2020-01-26 2022-11-01 Ramona Optics Inc. System and method for synchronized fluorescence capture

Also Published As

Publication number Publication date
EP4050396B1 (en) 2024-01-31
EP4050396A1 (en) 2022-08-31
CN116149038A (zh) 2023-05-23
US12436373B2 (en) 2025-10-07
US20230161143A1 (en) 2023-05-25

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