CN116149038A - 荧光显微镜系统和方法 - Google Patents

荧光显微镜系统和方法 Download PDF

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Publication number
CN116149038A
CN116149038A CN202211463492.9A CN202211463492A CN116149038A CN 116149038 A CN116149038 A CN 116149038A CN 202211463492 A CN202211463492 A CN 202211463492A CN 116149038 A CN116149038 A CN 116149038A
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CN
China
Prior art keywords
imaging mode
sample
fluorophores
control unit
different fluorophores
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202211463492.9A
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English (en)
Chinese (zh)
Inventor
凯·瑞特舍尔
本杰明·戴斯勒
丹尼斯·杰纳特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Microsystems CMS GmbH
Original Assignee
Leica Microsystems CMS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=78770400&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN116149038(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Leica Microsystems CMS GmbH filed Critical Leica Microsystems CMS GmbH
Publication of CN116149038A publication Critical patent/CN116149038A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N2021/0106—General arrangement of respective parts

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
CN202211463492.9A 2021-11-23 2022-11-22 荧光显微镜系统和方法 Pending CN116149038A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21209766.1A EP4050396B1 (en) 2021-11-23 2021-11-23 Fluorescence microscope system and method
EP21209766.1 2021-11-23

Publications (1)

Publication Number Publication Date
CN116149038A true CN116149038A (zh) 2023-05-23

Family

ID=78770400

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211463492.9A Pending CN116149038A (zh) 2021-11-23 2022-11-22 荧光显微镜系统和方法

Country Status (4)

Country Link
US (1) US12436373B2 (enExample)
EP (1) EP4050396B1 (enExample)
JP (1) JP2023076815A (enExample)
CN (1) CN116149038A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4092466A1 (en) * 2021-05-21 2022-11-23 Leica Microsystems CMS GmbH Method for obtaining an operating value of an illumination parameter for examining a fluorescent sample, method for examining a fluorescent sample, microscope system and computer program

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10222359B4 (de) 2002-05-21 2005-01-05 Max Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur spektral differenzierenden, bildgebenden Messung von Fluoreszenzlicht
JP2004013128A (ja) * 2002-06-11 2004-01-15 Olympus Corp 蛍光顕微鏡
JP4673000B2 (ja) * 2004-05-21 2011-04-20 株式会社キーエンス 蛍光顕微鏡、蛍光顕微鏡装置を使用した表示方法、蛍光顕微鏡画像表示プログラム及びコンピュータで読み取り可能な記録媒体並びに記憶した機器
JP2007199605A (ja) * 2006-01-30 2007-08-09 Osaka Prefecture Univ 蛍光顕微鏡
GB0707433D0 (en) * 2007-04-18 2007-05-23 Stfc Science & Technology Fluorescence measurement
JP2011252950A (ja) * 2010-05-31 2011-12-15 Nikon Corp 撮像装置および撮像プログラム
JP5714997B2 (ja) * 2011-07-14 2015-05-07 オリンパス株式会社 蛍光顕微鏡装置
JP6987831B2 (ja) * 2016-03-10 2022-01-05 シスメックス株式会社 光学機器および像の形成方法
US10768105B1 (en) * 2016-07-29 2020-09-08 Labrador Diagnostics Llc Image analysis and measurement of biological samples
US11487097B1 (en) * 2020-01-26 2022-11-01 Ramona Optics Inc. System and method for synchronized fluorescence capture

Also Published As

Publication number Publication date
EP4050396B1 (en) 2024-01-31
EP4050396A1 (en) 2022-08-31
JP2023076815A (ja) 2023-06-02
US12436373B2 (en) 2025-10-07
US20230161143A1 (en) 2023-05-25

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