JP2022520616A - クロマチン会合タンパク質の定量的マッピング - Google Patents

クロマチン会合タンパク質の定量的マッピング Download PDF

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JP2022520616A
JP2022520616A JP2021547435A JP2021547435A JP2022520616A JP 2022520616 A JP2022520616 A JP 2022520616A JP 2021547435 A JP2021547435 A JP 2021547435A JP 2021547435 A JP2021547435 A JP 2021547435A JP 2022520616 A JP2022520616 A JP 2022520616A
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nucleosome
polynucleosome
dna
library
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JP2022520616A5 (enExample
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コウルズ，マーティス・ダブルュー．
スゥン，ヅゥーウン
キーオ，マイケル－クリストファー
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Epicypher Inc
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Epicypher Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021547435A 2019-02-15 2020-02-14 クロマチン会合タンパク質の定量的マッピング Pending JP2022520616A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962806174P 2019-02-15 2019-02-15
US62/806,174 2019-02-15
PCT/US2020/018216 WO2020168151A2 (en) 2019-02-15 2020-02-14 Quantitative mapping of chromatin associated proteins

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JP2022520616A true JP2022520616A (ja) 2022-03-31
JP2022520616A5 JP2022520616A5 (enExample) 2023-02-13

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US (1) US20220049245A1 (enExample)
EP (1) EP3924507A4 (enExample)
JP (1) JP2022520616A (enExample)
CN (1) CN113677712A (enExample)
AU (1) AU2020223318A1 (enExample)
CA (1) CA3129907A1 (enExample)
WO (1) WO2020168151A2 (enExample)

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
GB202108185D0 (en) * 2021-06-08 2021-07-21 Belgian Volition Sprl Standardisation of nucleosome assays using biologically derived calibrants
US20250164503A1 (en) * 2022-02-15 2025-05-22 Epicypher, Inc. Engineered recombinant protein-binding domains as detection reagents
WO2024081782A1 (en) * 2022-10-12 2024-04-18 Epicypher, Inc. High efficiency antibodies for chromatin targets
WO2024130035A1 (en) * 2022-12-14 2024-06-20 The Regents Of The University Of California High-throughput mapping and normalization for genomic methods

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015521462A (ja) * 2012-06-06 2015-07-30 ザ、トラスティーズ オブ プリンストン ユニバーシティ デザイナーモノヌクレオソーム（ｄｅｓｉｇｎｅｒｍｏｎｏｎｕｃｌｅｏｓｏｍｅ）のＤＮＡバーコード化ならびにクロマチンリーダー、ライター、イレーサーおよびそのモジュレーターをプロファイリングするためのクロマチンアレイライブラリー
JP2017506073A (ja) * 2014-02-03 2017-03-02 ザ ユニバーシティー オブ シカゴ Ｄｎａ−蛋白質複合体の組成及びその密度の定量評価方法
JP2018082622A (ja) * 2015-02-25 2018-05-31 国立大学法人東北大学 免疫沈降用の内部標準分子及び免疫沈降方法
JP2018532373A (ja) * 2015-08-12 2018-11-08 ツェーエーエムエム・フォルシュングスツェントルム・フュア・モレクラーレ・メディツィン・ゲーエムベーハー 核酸の研究方法

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US6884870B2 (en) * 1998-03-20 2005-04-26 California Institute Of Technology Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells
US20020155114A1 (en) * 1998-08-31 2002-10-24 James D. Marks Therapeutic monoclonal antibodies that neutralize botulinum neurotoxins
EP1278875A1 (en) * 2000-05-05 2003-01-29 Imperial College Innovations Limited Crystallography methods
JP4061043B2 (ja) * 2000-12-28 2008-03-12 株式会社ポストゲノム研究所 ｉｎｖｉｔｒｏ転写／翻訳系によるペプチド等の製造方法
US20020192721A1 (en) * 2001-03-28 2002-12-19 Engeneos, Inc. Modular molecular clasps and uses thereof
WO2008060483A2 (en) * 2006-11-10 2008-05-22 Cellumen, Inc. Protein-protein interaction biosensors and methods of use thereof
ES2560871T3 (es) * 2007-07-10 2016-02-23 Apogenix Gmbh Proteínas de fusión de colectina de la superfamilia de TNF
CA3034369A1 (en) * 2016-08-19 2018-02-22 Whitehead Institute For Biomedical Research Methods of editing dna methylation
WO2018213719A1 (en) * 2017-05-19 2018-11-22 Epicypher, Inc. Assays for nucleosome remodeling activity

Patent Citations (4)

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JP2015521462A (ja) * 2012-06-06 2015-07-30 ザ、トラスティーズ オブ プリンストン ユニバーシティ デザイナーモノヌクレオソーム（ｄｅｓｉｇｎｅｒｍｏｎｏｎｕｃｌｅｏｓｏｍｅ）のＤＮＡバーコード化ならびにクロマチンリーダー、ライター、イレーサーおよびそのモジュレーターをプロファイリングするためのクロマチンアレイライブラリー
JP2017506073A (ja) * 2014-02-03 2017-03-02 ザ ユニバーシティー オブ シカゴ Ｄｎａ−蛋白質複合体の組成及びその密度の定量評価方法
JP2018082622A (ja) * 2015-02-25 2018-05-31 国立大学法人東北大学 免疫沈降用の内部標準分子及び免疫沈降方法
JP2018532373A (ja) * 2015-08-12 2018-11-08 ツェーエーエムエム・フォルシュングスツェントルム・フュア・モレクラーレ・メディツィン・ゲーエムベーハー 核酸の研究方法

Non-Patent Citations (2)

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Title
LISZCZAK, GLEN ET AL.: ""Acetylation blocks DNA damage-induced chromatin ADP-ribosylation"", NATURE CHEMICAL BIOLOGY, vol. 14, no. 9, JPN6024002916, 16 July 2018 (2018-07-16), pages 837 - 840, XP036572835, ISSN: 0005516590, DOI: 10.1038/s41589-018-0097-1 *
NGUYEN, UYEN T. T. ET AL.: ""Accelerated chromatin biochemistry using DNA-barcoded nucleosome libraries"", NATURE METHODS, vol. 11, no. 8, JPN6024002917, 6 July 2014 (2014-07-06), pages 834 - 840, ISSN: 0005516589 *

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Publication number Publication date
WO2020168151A3 (en) 2020-10-29
AU2020223318A1 (en) 2021-09-16
EP3924507A4 (en) 2022-11-30
US20220049245A1 (en) 2022-02-17
CA3129907A1 (en) 2020-08-20
WO2020168151A2 (en) 2020-08-20
EP3924507A2 (en) 2021-12-22
CN113677712A (zh) 2021-11-19

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