CA3129907A1 - Quantitative mapping of chromatin associated proteins - Google Patents
Quantitative mapping of chromatin associated proteins Download PDFInfo
- Publication number
- CA3129907A1 CA3129907A1 CA3129907A CA3129907A CA3129907A1 CA 3129907 A1 CA3129907 A1 CA 3129907A1 CA 3129907 A CA3129907 A CA 3129907A CA 3129907 A CA3129907 A CA 3129907A CA 3129907 A1 CA3129907 A1 CA 3129907A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleosome
- chap
- polynucleosome
- library
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962806174P | 2019-02-15 | 2019-02-15 | |
| US62/806,174 | 2019-02-15 | ||
| PCT/US2020/018216 WO2020168151A2 (en) | 2019-02-15 | 2020-02-14 | Quantitative mapping of chromatin associated proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3129907A1 true CA3129907A1 (en) | 2020-08-20 |
Family
ID=72044800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3129907A Pending CA3129907A1 (en) | 2019-02-15 | 2020-02-14 | Quantitative mapping of chromatin associated proteins |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20220049245A1 (enExample) |
| EP (1) | EP3924507A4 (enExample) |
| JP (1) | JP2022520616A (enExample) |
| CN (1) | CN113677712A (enExample) |
| AU (1) | AU2020223318A1 (enExample) |
| CA (1) | CA3129907A1 (enExample) |
| WO (1) | WO2020168151A2 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB202108185D0 (en) * | 2021-06-08 | 2021-07-21 | Belgian Volition Sprl | Standardisation of nucleosome assays using biologically derived calibrants |
| US20250164503A1 (en) * | 2022-02-15 | 2025-05-22 | Epicypher, Inc. | Engineered recombinant protein-binding domains as detection reagents |
| WO2024081782A1 (en) * | 2022-10-12 | 2024-04-18 | Epicypher, Inc. | High efficiency antibodies for chromatin targets |
| WO2024130035A1 (en) * | 2022-12-14 | 2024-06-20 | The Regents Of The University Of California | High-throughput mapping and normalization for genomic methods |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6884870B2 (en) * | 1998-03-20 | 2005-04-26 | California Institute Of Technology | Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells |
| US20020155114A1 (en) * | 1998-08-31 | 2002-10-24 | James D. Marks | Therapeutic monoclonal antibodies that neutralize botulinum neurotoxins |
| EP1278875A1 (en) * | 2000-05-05 | 2003-01-29 | Imperial College Innovations Limited | Crystallography methods |
| JP4061043B2 (ja) * | 2000-12-28 | 2008-03-12 | 株式会社ポストゲノム研究所 | ｉｎｖｉｔｒｏ転写／翻訳系によるペプチド等の製造方法 |
| US20020192721A1 (en) * | 2001-03-28 | 2002-12-19 | Engeneos, Inc. | Modular molecular clasps and uses thereof |
| WO2008060483A2 (en) * | 2006-11-10 | 2008-05-22 | Cellumen, Inc. | Protein-protein interaction biosensors and methods of use thereof |
| ES2560871T3 (es) * | 2007-07-10 | 2016-02-23 | Apogenix Gmbh | Proteínas de fusión de colectina de la superfamilia de TNF |
| WO2013184930A2 (en) * | 2012-06-06 | 2013-12-12 | Trustees Of Princeton University | Dna barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof |
| WO2015117145A1 (en) * | 2014-02-03 | 2015-08-06 | The University Of Chicago | Compositions and methods for quantitative assesment of dna-protein complex density |
| JP2018082622A (ja) * | 2015-02-25 | 2018-05-31 | 国立大学法人東北大学 | 免疫沈降用の内部標準分子及び免疫沈降方法 |
| LT3334841T (lt) * | 2015-08-12 | 2020-02-10 | Cemm - Forschungszentrum Für Molekulare Medizin Gmbh | Nukleorūgščių tyrimo būdai |
| CA3034369A1 (en) * | 2016-08-19 | 2018-02-22 | Whitehead Institute For Biomedical Research | Methods of editing dna methylation |
| WO2018213719A1 (en) * | 2017-05-19 | 2018-11-22 | Epicypher, Inc. | Assays for nucleosome remodeling activity |
-
2020
- 2020-02-14 EP EP20755190.4A patent/EP3924507A4/en active Pending
- 2020-02-14 JP JP2021547435A patent/JP2022520616A/ja active Pending
- 2020-02-14 CN CN202080026856.XA patent/CN113677712A/zh active Pending
- 2020-02-14 CA CA3129907A patent/CA3129907A1/en active Pending
- 2020-02-14 AU AU2020223318A patent/AU2020223318A1/en active Pending
- 2020-02-14 US US17/430,741 patent/US20220049245A1/en active Pending
- 2020-02-14 WO PCT/US2020/018216 patent/WO2020168151A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020168151A3 (en) | 2020-10-29 |
| AU2020223318A1 (en) | 2021-09-16 |
| EP3924507A4 (en) | 2022-11-30 |
| US20220049245A1 (en) | 2022-02-17 |
| WO2020168151A2 (en) | 2020-08-20 |
| EP3924507A2 (en) | 2021-12-22 |
| JP2022520616A (ja) | 2022-03-31 |
| CN113677712A (zh) | 2021-11-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220826 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD Year of fee payment: 5 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20241223 |
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| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT Effective date: 20241223 Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20241223 |
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| D15 | Examination report completed |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT Effective date: 20250203 |
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| B12 | Application deemed to be withdrawn, abandoned or lapsed |
Free format text: ST27 STATUS EVENT CODE: N-2-6-B10-B12-B303 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO AN EXAMINER'S REQUISITION Effective date: 20250603 |
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| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20260330 |