JP2022513123A - 傷痍治療用医薬組成物 - Google Patents
傷痍治療用医薬組成物 Download PDFInfo
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- JP2022513123A JP2022513123A JP2021529098A JP2021529098A JP2022513123A JP 2022513123 A JP2022513123 A JP 2022513123A JP 2021529098 A JP2021529098 A JP 2021529098A JP 2021529098 A JP2021529098 A JP 2021529098A JP 2022513123 A JP2022513123 A JP 2022513123A
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Abstract
Description
HIV、HCV及びHBVを含む可能な病源体に対して検査した結果において陰性と判定されたヒト由来の新鮮な血液(中央血液院)を30℃の水槽で解凍させた後、3000rpmで10分間遠心分離し、赤血球及び白血球などの沈殿物を除外した上層の淡黄色の血漿を分離した。
実施例1で得た商用成分が除去された血漿分画は、次の3つの方法を連続して行って、血漿成分中に存在し得るウイルスを不活性化させた。
フィブリノーゲン/トロンビン/アルブミン除去及びシルクタンパク質ペプチド追加の血漿分画は、15℃でコバルト源(60Co)を用いて1.8kGy/hrの強度で合計25kGyのガンマ線を照射した。
ガンマ線照射されたフィブリノーゲン/トロンビン/アルブミン除去及びシルクタンパク質ペプチド追加の血漿分画に、最終濃度1μM濃度でメチレンブルー(Methylene blue)を添加し、60,000luxで1時間白色光を照射した。残余メチレンブルーは濾過して除去し、-80℃で8時間凍結させ、-48℃で7日間乾燥させて凍結乾燥した。
フィブリノーゲン/トロンビン/アルブミン除去及びシルクタンパク質ペプチド追加の血漿分画は、篩にかけて濾過し、粉砕して均質化させた後、ステンレススチールタンク中で水分含有量が8%(w/w)となるように水蒸気を徐々に注入した。前記水蒸気処理された血漿を、乾燥窒素で充填されたステンレススチールシリンダーに移して酸素を除去した後、60℃で10時間加熱し、ウイルスが不活性化された血漿分画を得た。
実施例2で得られた、ウイルスが不活性化された血漿分画を、1N HCl(塩酸)又は1N NaOH(水酸化ナトリウム)を添加して撹拌しながらpH測定機(Orion)でpHを測定してpH5.5に調整し、凍結乾燥させて血漿分画物を得た。
実施例3で製造した凍結乾燥血漿分画の傷治療効果を確認するために、ネズミの線維芽細胞であるNIH3T3細胞株(KCTC 4612)を用いて傷再生確認実験を行った。細胞培養プレートにNIH3T3細胞を50万/プレート濃度でシードし、DMEM培地(DMEM,Gibco)に10%ウシ胎児血清(FBS,Thermo Fisher)と1%ペニシリンストレプトマイシン(Pen-strep,Sigma-Aldrich))を混合した細胞成長用培地で1週間コンフルエントに細胞が成長するようにした。
実施例3で得られた凍結乾燥血漿分画とセリシンタンパク質(Sigma-Aldrich)を含有する創傷治療製剤の傷治療効果を確認するために、実施例4と同様に、NIH3T3細胞株を用いて傷再生確認実験を行った。実施例4と同様に、10% FBS含有細胞成長用培地でコンフルエントに細胞を成長させ、1日間、無血清培地を用いて細胞を餓死させた後、傷治療効果を確認するために、プレート上に成長した細胞にかき傷をつけ、無血清培地に血漿成分を0.5%の濃度で添加し、ここにセリシンタンパク質をそれぞれ0.05%(w/v)及び0.5%(w/v)の濃度で添加した。対照群として無血清培地だけを処理した群と血漿成分だけを含有する群を用いて実験を行った。これらの実験群を12時間培養後に、かき傷が治癒されたか否かを確認した。
実施例3で得た凍結乾燥血漿分画と組換えシルクタンパク質を含有する製剤の傷治療効果を確認するために、実施例4と同じ方法でNIH3T3細胞株を用いて傷再生確認実験を行った。
Claims (9)
- 血漿又は血清成分とシルクタンパク質とを有効成分として含有する、創傷治療用医薬組成物。
- 前記血漿又は血清は、ヒト又は家畜から由来したものであることを特徴とする、請求項1に記載の組成物。
- 前記シルクタンパク質は、自然由来又は組換えシルクタンパク質由来のものであることを特徴とする、請求項1に記載の組成物。
- 前記シルクタンパク質は、ドラグラインシルク(dragline silk)、エラスチン(elastin)、シルクフィブロイン(silk fibroin)、ビサス(byssus)、セリシン(sericin)、鞭毛状シルク(flagelliform silk)及びコラーゲン(collagen)からなる群から選ばれるタンパク質を構成する反復単位ペプチドで構成されることを特徴とする、請求項1に記載の組成物。
- 前記シルクタンパク質は、グリシン或いはセリン含有量が0.001%以上であるペプチドが1~160回反復されている構造を有することを特徴とする、請求項1に記載の組成物。
- 創傷は、非治癒外傷性創傷、放射線照射による組織の破壊、擦過傷、骨壞疽、裂傷、剥脱創、貫通傷、銃創、切創、火傷、凍傷、皮膚潰瘍、皮膚乾燥、皮膚角化症、ひび、あかぎれ、皮膚炎、皮膚糸状菌症による痛み、手術上又は血管疾患創傷、打撲傷、角膜創傷、床擦れ、臥瘡、糖尿性皮膚糜爛のような糖尿病及び循環不良に関連した状態、慢性潰瘍、整形手術後縫合部位、脊椎傷害性創傷、婦人科的創傷、化学的創傷及びニキビからなる群から選ばれるものであることを特徴とする、請求項1に記載の組成物。
- クリーム、軟膏、ゲル、液剤、粉末剤及びパッチからなる群から選ばれるいずれか一つの形態であることを特徴とする、請求項1に記載の組成物。
- pHが3.5~6.5であることを特徴とする、請求項1に記載の組成物。
- 血漿成分は、凍結乾燥製剤であることを特徴とする、請求項1に記載の組成物。
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