JP2022509868A - ランダムオリゴヌクレオチドの生成方法およびその配列決定方法 - Google Patents

ランダムオリゴヌクレオチドの生成方法およびその配列決定方法 Download PDF

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JP2022509868A
JP2022509868A JP2021531315A JP2021531315A JP2022509868A JP 2022509868 A JP2022509868 A JP 2022509868A JP 2021531315 A JP2021531315 A JP 2021531315A JP 2021531315 A JP2021531315 A JP 2021531315A JP 2022509868 A JP2022509868 A JP 2022509868A
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random
oligonucleotides
oligonucleotide
random oligonucleotide
sequence
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JP2022509868A5 (cg-RX-API-DMAC7.html
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サワヤ，スターリン
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ジェネインフォセック、インコーポレイテッド
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    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C07H1/02—Phosphorylation
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    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
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    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/314—Phosphoramidates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2021531315A 2018-11-30 2019-11-30 ランダムオリゴヌクレオチドの生成方法およびその配列決定方法 Pending JP2022509868A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862773671P 2018-11-30 2018-11-30
US62/773,671 2018-11-30
PCT/US2019/063890 WO2020113195A1 (en) 2018-11-30 2019-11-30 A method for generating random oligonucleotides and determining their sequence

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JP2022509868A true JP2022509868A (ja) 2022-01-24
JP2022509868A5 JP2022509868A5 (cg-RX-API-DMAC7.html) 2022-07-01

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US (1) US20220010306A1 (cg-RX-API-DMAC7.html)
EP (1) EP3887385B1 (cg-RX-API-DMAC7.html)
JP (1) JP2022509868A (cg-RX-API-DMAC7.html)
KR (1) KR20210098491A (cg-RX-API-DMAC7.html)
CN (1) CN113166192A (cg-RX-API-DMAC7.html)
CA (1) CA3120107A1 (cg-RX-API-DMAC7.html)
IL (1) IL283364B1 (cg-RX-API-DMAC7.html)
WO (1) WO2020113195A1 (cg-RX-API-DMAC7.html)

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CN115925774B (zh) * 2022-12-22 2024-06-14 百力格生物科技(上海)股份有限公司 合成特异性分子标签的方法及其分子标签接头

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001521040A (ja) * 1997-10-28 2001-11-06 イスチチュート デイ リセルシュ デイ バイオロジア モレコラレ ピー．アンジェレッティ ソシエタ ペル アチオニ 全体にまたは部分的にランダムな配列を有するポリヌクレオチドの合成方法
JP2005500841A (ja) * 2001-07-27 2005-01-13 アメリカ合衆国 オリゴヌクレオチドを用いるインビボ部位指定変異誘発のためのシステム
JP2010142250A (ja) * 1998-12-14 2010-07-01 Pacific Biosciences Of California Inc ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法
JP2016527313A (ja) * 2013-08-05 2016-09-08 ツイスト バイオサイエンス コーポレーション 新規合成した遺伝子ライブラリ
JP2016533187A (ja) * 2013-08-28 2016-10-27 セルラー リサーチ， インコーポレイテッド 大規模並列単一細胞分析
WO2017176541A1 (en) * 2016-04-04 2017-10-12 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis
JP2018510662A (ja) * 2015-04-10 2018-04-19 スペーシャル トランスクリプトミクス アクチボラグ 生物学的試料の空間識別されるマルチプレックスな核酸分析

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4313861B2 (ja) 1997-08-01 2009-08-12 キヤノン株式会社 プローブアレイの製造方法
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
US6545758B1 (en) 2000-08-17 2003-04-08 Perry Sandstrom Microarray detector and synthesizer
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
WO2003078623A1 (en) * 2002-03-19 2003-09-25 Fujitsu Limited Functional molecule and process for producing the same
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
WO2007040592A1 (en) 2005-03-10 2007-04-12 Gao, Xiaolian Making nucleic acid sequences in parallel and use
US7572907B2 (en) 2005-04-29 2009-08-11 Agilent Technologies, Inc. Methods and compounds for polynucleotide synthesis
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
WO2010057185A1 (en) 2008-11-17 2010-05-20 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
AU2015209079B2 (en) 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
US20150211061A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods for determining a nucleotide sequence
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001521040A (ja) * 1997-10-28 2001-11-06 イスチチュート デイ リセルシュ デイ バイオロジア モレコラレ ピー．アンジェレッティ ソシエタ ペル アチオニ 全体にまたは部分的にランダムな配列を有するポリヌクレオチドの合成方法
JP2010142250A (ja) * 1998-12-14 2010-07-01 Pacific Biosciences Of California Inc ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法
JP2005500841A (ja) * 2001-07-27 2005-01-13 アメリカ合衆国 オリゴヌクレオチドを用いるインビボ部位指定変異誘発のためのシステム
JP2016527313A (ja) * 2013-08-05 2016-09-08 ツイスト バイオサイエンス コーポレーション 新規合成した遺伝子ライブラリ
JP2016533187A (ja) * 2013-08-28 2016-10-27 セルラー リサーチ， インコーポレイテッド 大規模並列単一細胞分析
JP2018510662A (ja) * 2015-04-10 2018-04-19 スペーシャル トランスクリプトミクス アクチボラグ 生物学的試料の空間識別されるマルチプレックスな核酸分析
WO2017176541A1 (en) * 2016-04-04 2017-10-12 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SCIENTIFIC REPORTS, vol. Vol.5, No.9740, pp.1-5, JPN6023021530, 2015, ISSN: 0005221767 *

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CN113166192A (zh) 2021-07-23
CA3120107A1 (en) 2020-06-04
EP3887385A4 (en) 2022-08-24
US20220010306A1 (en) 2022-01-13
IL283364A (en) 2021-07-29
IL283364B1 (en) 2026-05-01
EP3887385C0 (en) 2024-03-20
EP3887385A1 (en) 2021-10-06
WO2020113195A1 (en) 2020-06-04
KR20210098491A (ko) 2021-08-10
EP3887385B1 (en) 2024-03-20

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