CA3120107A1 - A method for generating random oligonucleotides and determining their sequence - Google Patents
A method for generating random oligonucleotides and determining their sequence Download PDFInfo
- Publication number
- CA3120107A1 CA3120107A1 CA3120107A CA3120107A CA3120107A1 CA 3120107 A1 CA3120107 A1 CA 3120107A1 CA 3120107 A CA3120107 A CA 3120107A CA 3120107 A CA3120107 A CA 3120107A CA 3120107 A1 CA3120107 A1 CA 3120107A1
- Authority
- CA
- Canada
- Prior art keywords
- random
- oligonucleotides
- oligonucleotide
- generated
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/02—Phosphorylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/314—Phosphoramidates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862773671P | 2018-11-30 | 2018-11-30 | |
| US62/773,671 | 2018-11-30 | ||
| PCT/US2019/063890 WO2020113195A1 (en) | 2018-11-30 | 2019-11-30 | A method for generating random oligonucleotides and determining their sequence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3120107A1 true CA3120107A1 (en) | 2020-06-04 |
Family
ID=70852676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3120107A Pending CA3120107A1 (en) | 2018-11-30 | 2019-11-30 | A method for generating random oligonucleotides and determining their sequence |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20220010306A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3887385B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2022509868A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20210098491A (cg-RX-API-DMAC7.html) |
| CN (1) | CN113166192A (cg-RX-API-DMAC7.html) |
| CA (1) | CA3120107A1 (cg-RX-API-DMAC7.html) |
| IL (1) | IL283364B1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2020113195A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115925774B (zh) * | 2022-12-22 | 2024-06-14 | 百力格生物科技(上海)股份有限公司 | 合成特异性分子标签的方法及其分子标签接头 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4313861B2 (ja) | 1997-08-01 | 2009-08-12 | キヤノン株式会社 | プローブアレイの製造方法 |
| IT1295830B1 (it) * | 1997-10-28 | 1999-05-28 | Angeletti P Ist Richerche Bio | Procedimento per la sintesi di polinucleotidi aventi sequenze nucleotidiche totalmente o parzialmente casuali |
| US6458583B1 (en) | 1998-09-09 | 2002-10-01 | Agilent Technologies, Inc. | Method and apparatus for making nucleic acid arrays |
| WO2000036152A1 (en) * | 1998-12-14 | 2000-06-22 | Li-Cor, Inc. | A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis |
| US6221653B1 (en) | 1999-04-27 | 2001-04-24 | Agilent Technologies, Inc. | Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids |
| US6077674A (en) | 1999-10-27 | 2000-06-20 | Agilent Technologies Inc. | Method of producing oligonucleotide arrays with features of high purity |
| US6545758B1 (en) | 2000-08-17 | 2003-04-08 | Perry Sandstrom | Microarray detector and synthesizer |
| EP1421187B1 (en) * | 2001-07-27 | 2007-10-10 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| US7157229B2 (en) | 2002-01-31 | 2007-01-02 | Nimblegen Systems, Inc. | Prepatterned substrate for optical synthesis of DNA probes |
| WO2003078623A1 (en) * | 2002-03-19 | 2003-09-25 | Fujitsu Limited | Functional molecule and process for producing the same |
| US7315019B2 (en) | 2004-09-17 | 2008-01-01 | Pacific Biosciences Of California, Inc. | Arrays of optical confinements and uses thereof |
| WO2007040592A1 (en) | 2005-03-10 | 2007-04-12 | Gao, Xiaolian | Making nucleic acid sequences in parallel and use |
| US7572907B2 (en) | 2005-04-29 | 2009-08-11 | Agilent Technologies, Inc. | Methods and compounds for polynucleotide synthesis |
| JP2011515102A (ja) | 2008-03-28 | 2011-05-19 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | 核酸シーケンシング用組成物及び方法 |
| WO2010057185A1 (en) | 2008-11-17 | 2010-05-20 | Pacific Biosciences Of California, Inc. | Phospholink nucleotides for sequencing applications |
| KR102160389B1 (ko) * | 2013-08-05 | 2020-09-28 | 트위스트 바이오사이언스 코포레이션 | 드 노보 합성된 유전자 라이브러리 |
| CN105705659B (zh) * | 2013-08-28 | 2019-11-29 | 贝克顿迪金森公司 | 大规模平行单细胞分析 |
| AU2015209079B2 (en) | 2014-01-27 | 2021-07-15 | Archerdx, Llc | Methods of preparing nucleic acids for sequencing |
| US20150211061A1 (en) | 2014-01-27 | 2015-07-30 | The General Hospital Corporation | Methods for determining a nucleotide sequence |
| EP4321627A3 (en) * | 2015-04-10 | 2024-04-17 | 10x Genomics Sweden AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| CN109312493B (zh) * | 2016-04-04 | 2022-08-09 | 哈佛学院董事及会员团体 | 酶促核酸合成 |
| CN110312825A (zh) | 2016-10-24 | 2019-10-08 | 吉内恩福赛克公司 | 隐藏核酸内存在的信息 |
-
2019
- 2019-11-30 EP EP19890138.1A patent/EP3887385B1/en active Active
- 2019-11-30 CN CN201980078595.3A patent/CN113166192A/zh active Pending
- 2019-11-30 JP JP2021531315A patent/JP2022509868A/ja active Pending
- 2019-11-30 WO PCT/US2019/063890 patent/WO2020113195A1/en not_active Ceased
- 2019-11-30 US US17/293,821 patent/US20220010306A1/en active Pending
- 2019-11-30 CA CA3120107A patent/CA3120107A1/en active Pending
- 2019-11-30 IL IL283364A patent/IL283364B1/en unknown
- 2019-11-30 KR KR1020217019928A patent/KR20210098491A/ko not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CN113166192A (zh) | 2021-07-23 |
| EP3887385A4 (en) | 2022-08-24 |
| US20220010306A1 (en) | 2022-01-13 |
| JP2022509868A (ja) | 2022-01-24 |
| IL283364A (en) | 2021-07-29 |
| IL283364B1 (en) | 2026-05-01 |
| EP3887385C0 (en) | 2024-03-20 |
| EP3887385A1 (en) | 2021-10-06 |
| WO2020113195A1 (en) | 2020-06-04 |
| KR20210098491A (ko) | 2021-08-10 |
| EP3887385B1 (en) | 2024-03-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220333185A1 (en) | Methods and compositions for whole transcriptome amplification | |
| JP7050057B2 (ja) | 増幅二本鎖デオキシリボ核酸の作製方法ならびに該方法で使用する組成物及びキット | |
| CN110249057B (zh) | 用于空间标记和分析生物样本中的核酸的方法 | |
| CN103781918B (zh) | 用于组织样本中核酸的局部或空间检测的方法和产品 | |
| JP2022506546A (ja) | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 | |
| EP3350732A1 (en) | Methods for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and compositions for practicing the same | |
| US20200157600A1 (en) | Methods and compositions for whole transcriptome amplification | |
| JP2022540744A (ja) | 核酸の空間情報を検出するためのアレイ及び方法 | |
| CA2449366A1 (en) | Happier mapping | |
| WO2020247685A2 (en) | Methods of barcoding nucleic acid for detection and sequencing | |
| AU2020280104A1 (en) | Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein | |
| EP3887385B1 (en) | A method for generating random oligonucleotides and determining their sequence | |
| DK2456892T3 (en) | Procedure for sequencing of a polynukleotidskabelon | |
| CA3153256A1 (en) | Methods and systems for rna-seq profiling | |
| JP7636848B2 (ja) | 相補ｄｎａ鎖を増幅する方法 | |
| GB2492042A (en) | Selector oligonucleotide-based methods and probes for nucleic acid detection or enrichment | |
| CN112996925A (zh) | 用于crispr的靶标无关的向导rna | |
| JP2004529663A (ja) | Ｈａｐｐｉａｒマッピング |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20231128 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 5TH ANNIV.) - STANDARD Year of fee payment: 5 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20241122 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT Effective date: 20241122 Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20241122 |
|
| D15 | Examination report completed |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT Effective date: 20250224 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION Effective date: 20250411 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20250414 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT Effective date: 20250718 |
|
| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED Effective date: 20250718 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD Year of fee payment: 6 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20251121 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20251121 |
|
| D22 | Grant of ip right intended |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D22-D128 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: ALLOWANCE REQUIREMENTS DETERMINED COMPLIANT Effective date: 20260513 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT Effective date: 20260513 |
|
| D00 | Search and/or examination requested or commenced |
Free format text: ST27 STATUS EVENT CODE: A-4-4-D10-D00-D164 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: RESPONSE TO NOTICE OF ALLOWANCE Effective date: 20260610 |
|
| D22 | Grant of ip right intended |
Free format text: ST27 STATUS EVENT CODE: A-2-4-D10-D22-D143 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: PRE-GRANT Effective date: 20260615 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-4-4-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20260615 |