CN113166192A - 产生随机寡核苷酸并确定其序列的方法 - Google Patents

产生随机寡核苷酸并确定其序列的方法 Download PDF

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CN113166192A
CN113166192A CN201980078595.3A CN201980078595A CN113166192A CN 113166192 A CN113166192 A CN 113166192A CN 201980078595 A CN201980078595 A CN 201980078595A CN 113166192 A CN113166192 A CN 113166192A
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oligonucleotides
random
oligonucleotide
sequence
molecule
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Chinese (zh)
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斯特林·萨瓦亚
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Geneinfosec Inc
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Geneinfosec Inc
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
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  • Physics & Mathematics (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201980078595.3A 2018-11-30 2019-11-30 产生随机寡核苷酸并确定其序列的方法 Pending CN113166192A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862773671P 2018-11-30 2018-11-30
US62/773,671 2018-11-30
PCT/US2019/063890 WO2020113195A1 (en) 2018-11-30 2019-11-30 A method for generating random oligonucleotides and determining their sequence

Publications (1)

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CN113166192A true CN113166192A (zh) 2021-07-23

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US (1) US20220010306A1 (cg-RX-API-DMAC7.html)
EP (1) EP3887385B1 (cg-RX-API-DMAC7.html)
JP (1) JP2022509868A (cg-RX-API-DMAC7.html)
KR (1) KR20210098491A (cg-RX-API-DMAC7.html)
CN (1) CN113166192A (cg-RX-API-DMAC7.html)
CA (1) CA3120107A1 (cg-RX-API-DMAC7.html)
IL (1) IL283364B1 (cg-RX-API-DMAC7.html)
WO (1) WO2020113195A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024130904A1 (zh) * 2022-12-22 2024-06-27 百力格生物科技(上海)股份有限公司 合成特异性分子标签的方法及其分子标签接头

Citations (4)

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WO1999021873A2 (en) * 1997-10-28 1999-05-06 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
CN105637097A (zh) * 2013-08-05 2016-06-01 特韦斯特生物科学公司 从头合成的基因文库
CN105705659A (zh) * 2013-08-28 2016-06-22 赛卢拉研究公司 大规模平行单细胞分析
CN107532207A (zh) * 2015-04-10 2018-01-02 空间转录公司 生物样本的空间区别、多重核酸分析

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JP4313861B2 (ja) 1997-08-01 2009-08-12 キヤノン株式会社 プローブアレイの製造方法
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
WO2000036152A1 (en) * 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
US6545758B1 (en) 2000-08-17 2003-04-08 Perry Sandstrom Microarray detector and synthesizer
EP1421187B1 (en) * 2001-07-27 2007-10-10 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Systems for in vivo site-directed mutagenesis using oligonucleotides
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
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US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
WO2007040592A1 (en) 2005-03-10 2007-04-12 Gao, Xiaolian Making nucleic acid sequences in parallel and use
US7572907B2 (en) 2005-04-29 2009-08-11 Agilent Technologies, Inc. Methods and compounds for polynucleotide synthesis
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
WO2010057185A1 (en) 2008-11-17 2010-05-20 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
AU2015209079B2 (en) 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
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WO1999021873A2 (en) * 1997-10-28 1999-05-06 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
CN105637097A (zh) * 2013-08-05 2016-06-01 特韦斯特生物科学公司 从头合成的基因文库
CN105705659A (zh) * 2013-08-28 2016-06-22 赛卢拉研究公司 大规模平行单细胞分析
JP2016533187A (ja) * 2013-08-28 2016-10-27 セルラー リサーチ， インコーポレイテッド 大規模並列単一細胞分析
CN107532207A (zh) * 2015-04-10 2018-01-02 空间转录公司 生物样本的空间区别、多重核酸分析

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Publication number Priority date Publication date Assignee Title
WO2024130904A1 (zh) * 2022-12-22 2024-06-27 百力格生物科技(上海)股份有限公司 合成特异性分子标签的方法及其分子标签接头

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CA3120107A1 (en) 2020-06-04
EP3887385A4 (en) 2022-08-24
US20220010306A1 (en) 2022-01-13
JP2022509868A (ja) 2022-01-24
IL283364A (en) 2021-07-29
IL283364B1 (en) 2026-05-01
EP3887385C0 (en) 2024-03-20
EP3887385A1 (en) 2021-10-06
WO2020113195A1 (en) 2020-06-04
KR20210098491A (ko) 2021-08-10
EP3887385B1 (en) 2024-03-20

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