IL283364B1 - Method for producing random oligonucleotides and determining their sequence - Google Patents

Method for producing random oligonucleotides and determining their sequence

Info

Publication number
IL283364B1
IL283364B1 IL283364A IL28336421A IL283364B1 IL 283364 B1 IL283364 B1 IL 283364B1 IL 283364 A IL283364 A IL 283364A IL 28336421 A IL28336421 A IL 28336421A IL 283364 B1 IL283364 B1 IL 283364B1
Authority
IL
Israel
Prior art keywords
oligonucleotides
random
oligonucleotide
sequence
generated
Prior art date
Application number
IL283364A
Other languages
English (en)
Hebrew (he)
Other versions
IL283364A (en
Original Assignee
Geneinfosec Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geneinfosec Inc filed Critical Geneinfosec Inc
Publication of IL283364A publication Critical patent/IL283364A/en
Publication of IL283364B1 publication Critical patent/IL283364B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IL283364A 2018-11-30 2019-11-30 Method for producing random oligonucleotides and determining their sequence IL283364B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862773671P 2018-11-30 2018-11-30
PCT/US2019/063890 WO2020113195A1 (en) 2018-11-30 2019-11-30 A method for generating random oligonucleotides and determining their sequence

Publications (2)

Publication Number Publication Date
IL283364A IL283364A (en) 2021-07-29
IL283364B1 true IL283364B1 (en) 2026-05-01

Family

ID=70852676

Family Applications (1)

Application Number Title Priority Date Filing Date
IL283364A IL283364B1 (en) 2018-11-30 2019-11-30 Method for producing random oligonucleotides and determining their sequence

Country Status (8)

Country Link
US (1) US20220010306A1 (cg-RX-API-DMAC7.html)
EP (1) EP3887385B1 (cg-RX-API-DMAC7.html)
JP (1) JP2022509868A (cg-RX-API-DMAC7.html)
KR (1) KR20210098491A (cg-RX-API-DMAC7.html)
CN (1) CN113166192A (cg-RX-API-DMAC7.html)
CA (1) CA3120107A1 (cg-RX-API-DMAC7.html)
IL (1) IL283364B1 (cg-RX-API-DMAC7.html)
WO (1) WO2020113195A1 (cg-RX-API-DMAC7.html)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115925774B (zh) * 2022-12-22 2024-06-14 百力格生物科技(上海)股份有限公司 合成特异性分子标签的方法及其分子标签接头

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6350595B1 (en) * 1997-10-28 2002-02-26 Istituto Di Ricerche Di Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
US20090170074A1 (en) * 1998-12-14 2009-07-02 Li-Cor, Inc. System and method for nucleic acid sequencing by polymerase synthesis
US20160089651A1 (en) * 2013-08-05 2016-03-31 Twist Bioscience Corporation De novo synthesized gene libraries
WO2017176541A1 (en) * 2016-04-04 2017-10-12 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis

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JP4313861B2 (ja) 1997-08-01 2009-08-12 キヤノン株式会社 プローブアレイの製造方法
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
US6545758B1 (en) 2000-08-17 2003-04-08 Perry Sandstrom Microarray detector and synthesizer
EP1421187B1 (en) * 2001-07-27 2007-10-10 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Systems for in vivo site-directed mutagenesis using oligonucleotides
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
WO2003078623A1 (en) * 2002-03-19 2003-09-25 Fujitsu Limited Functional molecule and process for producing the same
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
WO2007040592A1 (en) 2005-03-10 2007-04-12 Gao, Xiaolian Making nucleic acid sequences in parallel and use
US7572907B2 (en) 2005-04-29 2009-08-11 Agilent Technologies, Inc. Methods and compounds for polynucleotide synthesis
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
WO2010057185A1 (en) 2008-11-17 2010-05-20 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
CN105705659B (zh) * 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
AU2015209079B2 (en) 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
US20150211061A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods for determining a nucleotide sequence
EP4321627A3 (en) * 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6350595B1 (en) * 1997-10-28 2002-02-26 Istituto Di Ricerche Di Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
US20090170074A1 (en) * 1998-12-14 2009-07-02 Li-Cor, Inc. System and method for nucleic acid sequencing by polymerase synthesis
US20160089651A1 (en) * 2013-08-05 2016-03-31 Twist Bioscience Corporation De novo synthesized gene libraries
JP2016527313A (ja) * 2013-08-05 2016-09-08 ツイスト バイオサイエンス コーポレーション 新規合成した遺伝子ライブラリ
WO2017176541A1 (en) * 2016-04-04 2017-10-12 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VIRNEKAS, B., GE, L., PLUCKTHUN, A., SCHNEIDER, K. C., WELLNHOFER, G. N., & MORONEY, S. E, TRINUCLEOTIDE PHOSPHORAMIDITES: IDEAL REAGENTS FOR THE SYNTHESIS OF MIXED OLIGONUCLEOTIDES FOR RANDOM MUTAGENESIS, 25 December 1994 (1994-12-25) *

Also Published As

Publication number Publication date
CN113166192A (zh) 2021-07-23
CA3120107A1 (en) 2020-06-04
EP3887385A4 (en) 2022-08-24
US20220010306A1 (en) 2022-01-13
JP2022509868A (ja) 2022-01-24
IL283364A (en) 2021-07-29
EP3887385C0 (en) 2024-03-20
EP3887385A1 (en) 2021-10-06
WO2020113195A1 (en) 2020-06-04
KR20210098491A (ko) 2021-08-10
EP3887385B1 (en) 2024-03-20

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