JP2021536250A5 - - Google Patents
Info
- Publication number
- JP2021536250A5 JP2021536250A5 JP2021512437A JP2021512437A JP2021536250A5 JP 2021536250 A5 JP2021536250 A5 JP 2021536250A5 JP 2021512437 A JP2021512437 A JP 2021512437A JP 2021512437 A JP2021512437 A JP 2021512437A JP 2021536250 A5 JP2021536250 A5 JP 2021536250A5
- Authority
- JP
- Japan
- Prior art keywords
- cas9 protein
- seq
- motif
- recombinant cas9
- kferq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862728184P | 2018-09-07 | 2018-09-07 | |
| US62/728,184 | 2018-09-07 | ||
| PCT/EP2019/073838 WO2020049158A1 (en) | 2018-09-07 | 2019-09-06 | Compositions and methods for improved nucleases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021536250A JP2021536250A (ja) | 2021-12-27 |
| JP2021536250A5 true JP2021536250A5 (https=) | 2022-09-05 |
| JP7649735B2 JP7649735B2 (ja) | 2025-03-21 |
Family
ID=67909383
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021512437A Active JP7649735B2 (ja) | 2018-09-07 | 2019-09-06 | 改善されたヌクレアーゼのための組成物及び方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US12516304B2 (https=) |
| EP (1) | EP3847251A1 (https=) |
| JP (1) | JP7649735B2 (https=) |
| CN (1) | CN112654702B (https=) |
| WO (1) | WO2020049158A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019032628A1 (en) | 2017-08-07 | 2019-02-14 | The Regents Of The University Of California | GENERATING PLATFORM FOR SAFE CELLULAR THERAPEUTIC AGENTS |
| WO2020170129A1 (en) * | 2019-02-19 | 2020-08-27 | Instituto De Biologia Experimental E Tecnológica (Ibet) | A method for selective loading of proteins into exosomes and products thereof |
| EP4133069A2 (en) * | 2020-04-08 | 2023-02-15 | Astrazeneca AB | Compositions and methods for improved site-specific modification |
| WO2023039592A1 (en) * | 2021-09-13 | 2023-03-16 | Board Of Regents, The University Of Texas System | Cas9 variants with improved specificity |
| CN114686456B (zh) * | 2022-05-10 | 2023-02-17 | 中山大学 | 基于双分子脱氨酶互补的碱基编辑系统及其应用 |
| CN115074387A (zh) * | 2022-07-29 | 2022-09-20 | 郑州创迈生物科技有限公司 | 一种针对chozn细胞高效率转染的方法 |
| US12060602B2 (en) * | 2023-01-10 | 2024-08-13 | Vedabio, Inc. | Sample splitting for multiplexed detection of nucleic acids without amplification |
| CN118638763B (zh) * | 2024-07-10 | 2025-03-25 | 北京大学 | 具有提高的稳定性的Cas9蛋白、试剂盒及提高Cas9蛋白稳定性的方法 |
| CN118649229B (zh) * | 2024-07-10 | 2025-04-22 | 北京大学 | 工程化Cas9蛋白及其制备方法和应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5543158A (en) | 1993-07-23 | 1996-08-06 | Massachusetts Institute Of Technology | Biodegradable injectable nanoparticles |
| US6007845A (en) | 1994-07-22 | 1999-12-28 | Massachusetts Institute Of Technology | Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers |
| US5855913A (en) | 1997-01-16 | 1999-01-05 | Massachusetts Instite Of Technology | Particles incorporating surfactants for pulmonary drug delivery |
| US5895309A (en) | 1998-02-09 | 1999-04-20 | Spector; Donald | Collapsible hula-hoop |
| JP2008078613A (ja) | 2006-08-24 | 2008-04-03 | Rohm Co Ltd | 窒化物半導体の製造方法及び窒化物半導体素子 |
| EP3269395A1 (en) | 2008-11-07 | 2018-01-17 | Massachusetts Institute Of Technology | Aminoalcohol lipidoids and uses thereof |
| US8889394B2 (en) | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
| US9193827B2 (en) | 2010-08-26 | 2015-11-24 | Massachusetts Institute Of Technology | Poly(beta-amino alcohols), their preparation, and uses thereof |
| EP2691443B1 (en) | 2011-03-28 | 2021-02-17 | Massachusetts Institute of Technology | Conjugated lipomers and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| AU2013293270B2 (en) | 2012-07-25 | 2018-08-16 | Massachusetts Institute Of Technology | Inducible DNA binding proteins and genome perturbation tools and applications thereof |
| CA2885894C (en) | 2012-09-27 | 2021-11-23 | University Of British Columbia | Peptide directed protein knockdown |
| CN113355357B (zh) | 2012-12-12 | 2024-12-03 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
| WO2014118272A1 (en) | 2013-01-30 | 2014-08-07 | Santaris Pharma A/S | Antimir-122 oligonucleotide carbohydrate conjugates |
| CN112301024A (zh) | 2013-03-15 | 2021-02-02 | 通用医疗公司 | 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性 |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| CA2913234A1 (en) | 2013-05-22 | 2014-11-27 | Northwestern University | Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| EP4194557A1 (en) | 2014-08-06 | 2023-06-14 | Institute for Basic Science | Genome editing using campylobacter jejuni crispr/cas system-derived rgen |
| US9650617B2 (en) | 2015-01-28 | 2017-05-16 | Pioneer Hi-Bred International. Inc. | CRISPR hybrid DNA/RNA polynucleotides and methods of use |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| TWI906646B (zh) * | 2015-06-18 | 2025-12-01 | 美商博得學院股份有限公司 | 降低脫靶效應的ｃｒｉｓｐｒ酶突變 |
| KR20260019012A (ko) | 2016-10-07 | 2026-02-09 | 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. | Ｓ. 피오게네스 ｃａｓ９ 돌연변이 유전자 및 이에 의해 암호화되는 폴리펩티드 |
-
2019
- 2019-09-06 CN CN201980058359.5A patent/CN112654702B/zh active Active
- 2019-09-06 EP EP19765993.1A patent/EP3847251A1/en active Pending
- 2019-09-06 US US17/273,498 patent/US12516304B2/en active Active
- 2019-09-06 WO PCT/EP2019/073838 patent/WO2020049158A1/en not_active Ceased
- 2019-09-06 JP JP2021512437A patent/JP7649735B2/ja active Active
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