JP7649735B2 - 改善されたヌクレアーゼのための組成物及び方法 - Google Patents

改善されたヌクレアーゼのための組成物及び方法 Download PDF

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JP7649735B2
JP7649735B2 JP2021512437A JP2021512437A JP7649735B2 JP 7649735 B2 JP7649735 B2 JP 7649735B2 JP 2021512437 A JP2021512437 A JP 2021512437A JP 2021512437 A JP2021512437 A JP 2021512437A JP 7649735 B2 JP7649735 B2 JP 7649735B2
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cas9
seq
protein
motif
kferq
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JP2021536250A (ja
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アンナ－リナ・カヴァッロ
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AstraZeneca AB
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
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  • Biophysics (AREA)
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  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021512437A 2018-09-07 2019-09-06 改善されたヌクレアーゼのための組成物及び方法 Active JP7649735B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862728184P 2018-09-07 2018-09-07
US62/728,184 2018-09-07
PCT/EP2019/073838 WO2020049158A1 (en) 2018-09-07 2019-09-06 Compositions and methods for improved nucleases

Publications (3)

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JP2021536250A JP2021536250A (ja) 2021-12-27
JP2021536250A5 JP2021536250A5 (https=) 2022-09-05
JP7649735B2 true JP7649735B2 (ja) 2025-03-21

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US (1) US12516304B2 (https=)
EP (1) EP3847251A1 (https=)
JP (1) JP7649735B2 (https=)
CN (1) CN112654702B (https=)
WO (1) WO2020049158A1 (https=)

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Publication number Priority date Publication date Assignee Title
WO2019032628A1 (en) 2017-08-07 2019-02-14 The Regents Of The University Of California GENERATING PLATFORM FOR SAFE CELLULAR THERAPEUTIC AGENTS
WO2020170129A1 (en) * 2019-02-19 2020-08-27 Instituto De Biologia Experimental E Tecnológica (Ibet) A method for selective loading of proteins into exosomes and products thereof
EP4133069A2 (en) * 2020-04-08 2023-02-15 Astrazeneca AB Compositions and methods for improved site-specific modification
WO2023039592A1 (en) * 2021-09-13 2023-03-16 Board Of Regents, The University Of Texas System Cas9 variants with improved specificity
CN114686456B (zh) * 2022-05-10 2023-02-17 中山大学 基于双分子脱氨酶互补的碱基编辑系统及其应用
CN115074387A (zh) * 2022-07-29 2022-09-20 郑州创迈生物科技有限公司 一种针对chozn细胞高效率转染的方法
US12060602B2 (en) * 2023-01-10 2024-08-13 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification
CN118638763B (zh) * 2024-07-10 2025-03-25 北京大学 具有提高的稳定性的Cas9蛋白、试剂盒及提高Cas9蛋白稳定性的方法
CN118649229B (zh) * 2024-07-10 2025-04-22 北京大学 工程化Cas9蛋白及其制备方法和应用

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WO2014047741A1 (en) 2012-09-27 2014-04-03 University Of British Columbia Peptide directed protein knockdown
JP2016534132A (ja) 2013-09-06 2016-11-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 機能的なヌクレアーゼのための送達システム
WO2016205613A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Crispr enzyme mutations reducing off-target effects
JP2017526387A (ja) 2014-08-06 2017-09-14 トゥールジェン インコーポレイテッド カンピロバクター・ジェジュニｃｒｉｓｐｒ／ｃａｓシステムに由来するｒｇｅｎを使用したゲノム編集
WO2018068053A2 (en) 2016-10-07 2018-04-12 Integrated Dna Technologies, Inc. S. pyogenes cas9 mutant genes and polypeptides encoded by same

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US5855913A (en) 1997-01-16 1999-01-05 Massachusetts Instite Of Technology Particles incorporating surfactants for pulmonary drug delivery
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JP2016534132A (ja) 2013-09-06 2016-11-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 機能的なヌクレアーゼのための送達システム
JP2017526387A (ja) 2014-08-06 2017-09-14 トゥールジェン インコーポレイテッド カンピロバクター・ジェジュニｃｒｉｓｐｒ／ｃａｓシステムに由来するｒｇｅｎを使用したゲノム編集
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WO2018068053A2 (en) 2016-10-07 2018-04-12 Integrated Dna Technologies, Inc. S. pyogenes cas9 mutant genes and polypeptides encoded by same

Also Published As

Publication number Publication date
EP3847251A1 (en) 2021-07-14
CN112654702B (zh) 2025-05-13
US20210198642A1 (en) 2021-07-01
CN112654702A (zh) 2021-04-13
JP2021536250A (ja) 2021-12-27
WO2020049158A1 (en) 2020-03-12
US12516304B2 (en) 2026-01-06

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