JP2021530993A5 - - Google Patents

Info

Publication number
JP2021530993A5
JP2021530993A5 JP2021500923A JP2021500923A JP2021530993A5 JP 2021530993 A5 JP2021530993 A5 JP 2021530993A5 JP 2021500923 A JP2021500923 A JP 2021500923A JP 2021500923 A JP2021500923 A JP 2021500923A JP 2021530993 A5 JP2021530993 A5 JP 2021530993A5
Authority
JP
Japan
Prior art keywords
optionally
optionally substituted
formula
labeled
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2021500923A
Other languages
English (en)
Japanese (ja)
Other versions
JP2021530993A (ja
JP7676302B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2019/041717 external-priority patent/WO2020014681A1/en
Publication of JP2021530993A publication Critical patent/JP2021530993A/ja
Publication of JP2021530993A5 publication Critical patent/JP2021530993A5/ja
Application granted granted Critical
Publication of JP7676302B2 publication Critical patent/JP7676302B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2021500923A 2018-07-13 2019-07-12 バイコンジュゲート可能な標識および使用方法 Active JP7676302B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862698006P 2018-07-13 2018-07-13
US62/698,006 2018-07-13
PCT/US2019/041717 WO2020014681A1 (en) 2018-07-13 2019-07-12 Biconjugatable labels and methods of use

Publications (3)

Publication Number Publication Date
JP2021530993A JP2021530993A (ja) 2021-11-18
JP2021530993A5 true JP2021530993A5 (enExample) 2022-07-28
JP7676302B2 JP7676302B2 (ja) 2025-05-14

Family

ID=67480391

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021500923A Active JP7676302B2 (ja) 2018-07-13 2019-07-12 バイコンジュゲート可能な標識および使用方法

Country Status (10)

Country Link
US (1) US12312377B2 (enExample)
EP (1) EP3820943A1 (enExample)
JP (1) JP7676302B2 (enExample)
KR (1) KR20210031935A (enExample)
CN (1) CN112739781B (enExample)
AU (2) AU2019300169A1 (enExample)
BR (1) BR112021000344A2 (enExample)
CA (1) CA3105715A1 (enExample)
MX (1) MX2021000470A (enExample)
WO (1) WO2020014681A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019023257A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
CN113614097A (zh) 2019-01-23 2021-11-05 宽腾矽公司 高强度标记的反应物组合物和测序方法
KR20230097103A (ko) * 2020-10-27 2023-06-30 퀀텀-에스아이 인코포레이티드 광표백 정보를 이용한 단일 분자 검출 시스템
CN119320418A (zh) * 2022-01-05 2025-01-17 大睿生物医药科技(上海)有限公司 具有核苷酸类似物的双链rna

Family Cites Families (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP1067134B1 (en) 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
DE4435728A1 (de) 1994-01-19 1995-07-20 Boehringer Mannheim Gmbh Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5597909A (en) 1994-08-25 1997-01-28 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use
DE19782089T1 (de) 1996-10-29 1999-12-23 Univ Nebraska At Lincoln Linco Verfahren zum Nachweisen von Punktmutationen in DNA unter Verwendung von Fluoreszenzenergieübergang
GB9716231D0 (en) 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
EP1042407B1 (en) * 1997-12-17 2001-09-12 Carnegie-Mellon University Rigidized trimethine cyanine dyes
US6133445A (en) * 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
JP3813818B2 (ja) 1998-05-01 2006-08-23 アリゾナ ボード オブ リージェンツ オリゴヌクレオチドおよびｄｎａ分子のヌクレオチド配列の決定方法
US6153442A (en) 1998-05-20 2000-11-28 Dade Behring Inc. Reagents and methods for specific binding assays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
WO2000036152A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
EP1681356B1 (en) 1999-05-19 2011-10-19 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423536B1 (en) 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
EP1287154A4 (en) 2000-06-07 2010-12-15 Pacific Biosciences California NUCLEOTIDES WITH CHARGE SWITCHING
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
EP2100971A3 (en) 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US7556797B2 (en) * 2000-10-16 2009-07-07 Mallinckrodt Inc. Minimally invasive physiological function monitoring agents
EP1385829A4 (en) 2001-03-12 2005-10-19 Affymetrix Inc NUCLEIC ACID MARKING COMPOUNDS
WO2003020734A2 (en) 2001-08-29 2003-03-13 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US9389227B2 (en) 2001-09-05 2016-07-12 Posco Solid substrate comprising array of dendrons and methods for using the same
WO2003043402A2 (en) 2001-10-19 2003-05-30 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
US20040054162A1 (en) 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
DE10154291B4 (de) 2001-11-05 2005-05-19 Hain Lifescience Gmbh Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2003089670A2 (en) 2002-04-22 2003-10-30 Lawler Joseph F Jr Reagents for monitoring nuclei acid amplification and methods of using same
WO2003102239A2 (en) 2002-05-31 2003-12-11 Secretary, Department Of Atomic Energy Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands
US7414117B2 (en) 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US7667024B2 (en) 2003-11-19 2010-02-23 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
GB0422551D0 (en) 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
EP1920072A2 (en) 2005-07-01 2008-05-14 Dako Denmark A/S In situ hybridization detection method
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
JP2009519041A (ja) 2005-12-12 2009-05-14 アメリカ合衆国 核酸配列決定のためのプローブおよび使用方法
CN101346472B (zh) 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型
WO2007082331A1 (en) 2006-01-20 2007-07-26 Starpharma Pty Limited Modified macromolecule
US20090104614A1 (en) 2006-03-15 2009-04-23 The Trustees Of The University Of Pennsylvania Quantitative molecular probes
US8084734B2 (en) 2006-05-26 2011-12-27 The George Washington University Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
GB0625678D0 (en) 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
CN101434988B (zh) 2007-11-16 2013-05-01 深圳华因康基因科技有限公司 一种高通量寡核苷酸测序方法
WO2009091847A2 (en) 2008-01-14 2009-07-23 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US20090214436A1 (en) 2008-02-18 2009-08-27 Washington University Dichromic fluorescent compounds
US7968702B2 (en) 2008-03-13 2011-06-28 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8906831B2 (en) 2008-03-31 2014-12-09 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
EP2271751B1 (en) 2008-03-31 2015-07-22 Pacific Biosciences of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
GB2461026B (en) 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
US8252910B2 (en) 2008-11-19 2012-08-28 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
JP2012509675A (ja) 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド 低分子ｒｎａを検出するための組成物および方法、ならびにその使用
CA2745197A1 (en) 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
KR101063981B1 (ko) 2009-01-12 2011-09-14 서강대학교산학협력단 자유 라디칼 개시제 및 이를 이용한 펩타이드 서열의 동정방법
DE102009013653B4 (de) 2009-03-18 2014-09-18 Bruker Daltonik Gmbh Protein-Sequenzierung mit MALDI-Massenspektrometrie
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US8771948B2 (en) 2009-04-03 2014-07-08 Sequenom, Inc. Nucleic acid preparation compositions and methods
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
ES2977359T3 (es) 2010-01-19 2024-08-22 Sirigen Ii Ltd Nuevos reactivos para la amplificación dirigida de la señal de un biomarcador
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
JP2013527769A (ja) 2010-05-06 2013-07-04 アイビス バイオサイエンシズ インコーポレイティッド 統合された試料調製システムおよび安定化酵素混合物
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US20120016128A1 (en) * 2010-07-16 2012-01-19 Zhenjun Diwu Rhodamine lactone phosphoramidites and polymers
US8669374B2 (en) 2010-08-25 2014-03-11 Gene Shen Functionalized cyanine dyes (PEG)
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2016069124A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
CN102329884B (zh) 2011-10-20 2013-05-08 东南大学 两核苷酸同时合成dna测序方法及其应用
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9435810B2 (en) 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
EP3008094B1 (en) 2013-06-14 2018-12-26 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
US9957291B2 (en) 2013-08-05 2018-05-01 Pacific Biosciences Of California, Inc. Protected fluorescent reagent compounds
JP2015073523A (ja) 2013-10-11 2015-04-20 国立大学法人 東京大学 核酸の検出方法、検出プローブ、マイクロアレイ、核酸検出キット、核酸‐検出プローブ‐捕捉プローブ複合体、核酸固定化担体、及び流体デバイス
CN103866010B (zh) 2014-02-28 2016-04-20 郭诚 一种基因测序的方法
US9970049B2 (en) 2014-04-15 2018-05-15 The United States Of America, As Represented By The Secretary Of The Navy Use of modular nucleic acid scaffolds to create nanoscale energy harvesting and focusing arrays
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
EP3250689B1 (en) * 2015-01-28 2020-11-04 The Regents of The University of California Methods and compositions for labeling a single-stranded target nucleic acid
US10150872B2 (en) 2015-02-04 2018-12-11 Pacific Biosciences Of California, Inc. Multimeric protected fluorescent reagents
TWI704229B (zh) 2015-05-20 2020-09-11 美商寬騰矽公司 核酸定序方法
CN105001292A (zh) 2015-07-14 2015-10-28 深圳市瀚海基因生物科技有限公司 一种光可断裂的荧光标记可逆终端化合物及其在dna或rna测序中的用途
WO2017063093A1 (en) 2015-10-16 2017-04-20 Andrew Emili Protein sequencing methods and reagents
US10814299B2 (en) 2015-11-18 2020-10-27 Pacific Biosciences Of California, Inc. Loading nucleic acids onto substrates
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
EP3458465B1 (en) 2016-05-20 2024-05-08 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
WO2019023257A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
EP3655408B1 (en) 2017-08-24 2021-07-14 The U.S.A. As Represented By The Secretary, Department Of Health And Human Services Conformational restriction of cyanine fluorophores in far-red and near-ir range
US10836798B2 (en) 2018-11-08 2020-11-17 Government Of The United States Of America, As Represented By The Secretary Of Commerce Amino acid-specific binder and selectively identifying an amino acid
CN113614097A (zh) 2019-01-23 2021-11-05 宽腾矽公司 高强度标记的反应物组合物和测序方法
WO2023137314A1 (en) 2022-01-12 2023-07-20 Quantum-Si Incorporated Labeled binding reagents and methods of use thereof

Similar Documents

Publication Publication Date Title
El-Sagheer et al. Click chemistry with DNA
JP7287942B2 (ja) 高強度標識反応物組成物および配列決定のための方法
JP4761086B2 (ja) 核酸、標識物質、核酸検出方法およびキット
JP5364380B2 (ja) ポリヌクレオチド標識試薬
US20240124918A1 (en) High intensity labeled reactant compositions and methods for sequencing
JPH09506859A (ja) 標識結合パートナーのためのピリミジン誘導体
JP2019521096A (ja) 核酸シーケンシングにとって有用なタグ付きマルチヌクレオチド
WO2013074601A1 (en) Gamma-pna miniprobes for fluorescent labeling
CN101631796A (zh) 具有由单核苷或单核苷酸衍生的结构的化合物、核酸、标记物以及核酸检测方法和试剂盒
JP5975524B2 (ja) 化合物、核酸、核酸の製造方法および核酸を製造するためのキット
EP0422090A1 (fr) Derives de nucleosides utilisables pour la synthese d'oligonucleotides marques, oligonucleotides obtenus a partir de ces derives et leur synthese.
JP2004203878A (ja) マンニトールおよびグルシトール誘導体
JP5467275B2 (ja) 電子移動抑制剤とその利用
JP5201639B2 (ja) Ｒｎａ選択的ハイブリダイズ試薬及びその利用
JPH05123195A (ja) 核酸のホモジニアス検定法
Shershov et al. Synthesis and characterization of novel zwitterionic heptamethine indocyanine fluorophores
US6838244B1 (en) Fluorescent oligonucleotides and uses thereof
WO2023070010A1 (en) Ultrabright dna nanostructures for biosensing
RU2582198C1 (ru) Аналоги природных дезоксирибонуклеозидтрифосфатов и рибонуклеозидтрифосфатов, содержащие репортёрные флуоресцентные группы, для использования в аналитической биоорганической химии
JP6621831B2 (ja) ヌクレオシド誘導体及びその利用
JP3400789B2 (ja) プローブ作成方法
Klimkowski The application of thiazole orange and artificial backbone modifications in the synthesis of fluorescent nucleic acids probes
JP2006075082A (ja) オリゴヌクレオチドの架橋反応
Misra et al. Synthesis of terminally labelled RNA sequences: Fluorescence and hybridisation study of RNA-DNA duplexes
HK1066222B (en) Mannitol and glucitol derivatives