JP2021526829A5 - - Google Patents
Info
- Publication number
- JP2021526829A5 JP2021526829A5 JP2020568998A JP2020568998A JP2021526829A5 JP 2021526829 A5 JP2021526829 A5 JP 2021526829A5 JP 2020568998 A JP2020568998 A JP 2020568998A JP 2020568998 A JP2020568998 A JP 2020568998A JP 2021526829 A5 JP2021526829 A5 JP 2021526829A5
- Authority
- JP
- Japan
- Prior art keywords
- aav
- raav particles
- feed composition
- concentration
- anion exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862684835P | 2018-06-14 | 2018-06-14 | |
| US62/684,835 | 2018-06-14 | ||
| PCT/US2019/037013 WO2019241535A2 (en) | 2018-06-14 | 2019-06-13 | Anion exchange chromatography for recombinant aav production |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021526829A JP2021526829A (ja) | 2021-10-11 |
| JP2021526829A5 true JP2021526829A5 (enExample) | 2022-06-20 |
| JP7385603B2 JP7385603B2 (ja) | 2023-11-22 |
Family
ID=67513723
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020568998A Active JP7385603B2 (ja) | 2018-06-14 | 2019-06-13 | 組換えａａｖ生成のためのアニオン交換クロマトグラフィー |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US12070702B2 (enExample) |
| EP (1) | EP3807405A2 (enExample) |
| JP (1) | JP7385603B2 (enExample) |
| KR (1) | KR102930150B1 (enExample) |
| CN (1) | CN112469822A (enExample) |
| AU (1) | AU2019285186B2 (enExample) |
| CA (1) | CA3102817A1 (enExample) |
| IL (2) | IL319002A (enExample) |
| WO (1) | WO2019241535A2 (enExample) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11028372B2 (en) | 2015-12-11 | 2021-06-08 | The Trustees Of The University Of Pennsylvania | Scalable purification method for AAVRH10 |
| EP3387138B1 (en) | 2015-12-11 | 2022-01-26 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav9 |
| ES2934848T3 (es) | 2015-12-11 | 2023-02-27 | Univ Pennsylvania | Método de purificación escalable para AAV8 |
| EP3833745A1 (en) | 2018-08-10 | 2021-06-16 | REGENXBIO Inc. | Scalable method for recombinant aav production |
| FI3953483T3 (fi) | 2019-04-11 | 2023-11-30 | Regenxbio Inc | Kokoekskluusiokromatografiamenetelmiä rekombinanttien adenoassosioituneiden viruskoostumusten karakterisoimiseksi |
| TW202332458A (zh) | 2019-04-19 | 2023-08-16 | 美商銳進科斯生物股份有限公司 | 腺相關病毒載體調配物及方法 |
| WO2021158915A1 (en) * | 2020-02-06 | 2021-08-12 | Precision Biosciences, Inc. | Recombinant adeno-associated virus compositions and methods for producing and using the same |
| CA3190596A1 (en) * | 2020-08-07 | 2022-02-10 | Janssen Biotech, Inc. | Formulations for highly purified viral particles |
| US20230302381A1 (en) * | 2020-08-18 | 2023-09-28 | Sartorius Bia Separations D.O.O. | Metal-affinity extraction of host cell dna |
| AU2021356684A1 (en) | 2020-10-09 | 2023-05-11 | The Trustees Of The University Of Pennsylvania | Compositions and methods for treatment of fabry disease |
| WO2022082109A1 (en) | 2020-10-18 | 2022-04-21 | The Trustees Of The University Of Pennsylvania | Improved adeno-associated virus (aav) vector and uses therefor |
| JP2023550581A (ja) | 2020-10-29 | 2023-12-04 | ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア | Ａａｖカプシド及びそれを含有する組成物 |
| AU2021375404A1 (en) * | 2020-11-03 | 2023-06-08 | Pfizer Inc. | Methods for purification of aav vectors by anion exchange chromatography |
| EP4263841A1 (en) | 2020-12-16 | 2023-10-25 | REGENXBIO Inc. | Method of producing a recombinant adeno-associated virus particle |
| JP2024503895A (ja) | 2021-01-21 | 2024-01-29 | リジェネクスバイオ インコーポレイテッド | 組換えポリペプチド及びウイルスの生成の改善 |
| US20240084268A1 (en) * | 2021-01-21 | 2024-03-14 | Asklepios Biopharmaceutical, Inc. | Method for purifying recombinant viral particles |
| JP7683230B2 (ja) * | 2021-01-27 | 2025-05-27 | 東ソー株式会社 | アデノ随伴ウイルスの精製方法 |
| EP4299751A4 (en) * | 2021-03-09 | 2025-08-13 | Japan Chem Res | METHOD FOR PRODUCING A RECOMBINANT AAV9 VIRION |
| WO2023060113A1 (en) | 2021-10-05 | 2023-04-13 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| CN118202060A (zh) | 2021-10-05 | 2024-06-14 | 再生生物股份有限公司 | 用于重组aav生产的组合物和方法 |
| US20240408157A1 (en) | 2021-12-02 | 2024-12-12 | The Trustees Of The University Of Pennsylvania | Compositions and methods for treatment of fabry disease |
| CN114317464B (zh) * | 2021-12-27 | 2023-07-04 | 武汉汇研生物科技股份有限公司 | 腺相关病毒rAAV9的分离纯化方法 |
| US20250213727A1 (en) | 2022-03-25 | 2025-07-03 | Regenxbio Inc. | Dominant-negative tumor necrosis factor alpha adeno-associated virus gene therapy |
| US20230303983A1 (en) * | 2022-03-28 | 2023-09-28 | President And Fellows Of Harvard College | High efficiency purification of divergent aav serotypes using aavx affinity chromatography |
| WO2023239627A2 (en) | 2022-06-08 | 2023-12-14 | Regenxbio Inc. | Methods for recombinant aav production |
| US20260021205A1 (en) | 2022-07-15 | 2026-01-22 | The Trustees Of The University Of Pennsylvania | Recombinant aav having aav clade d and clade e capsids and compositions containing same |
| WO2024038365A1 (en) * | 2022-08-16 | 2024-02-22 | Pfizer Inc. | Methods for purification of aav vectors by anion exchange chromatography |
| WO2024211780A1 (en) | 2023-04-07 | 2024-10-10 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| WO2024233529A2 (en) | 2023-05-07 | 2024-11-14 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| WO2024242150A1 (ja) * | 2023-05-23 | 2024-11-28 | 東ソー株式会社 | 改変された組換えアデノ随伴ウイルス（ａａｖ）結合性タンパク質およびａａｖの精製方法 |
| KR20260019521A (ko) * | 2023-05-25 | 2026-02-10 | 주노 쎄러퓨티크스 인코퍼레이티드 | Aav 정제 방법 |
| WO2024263705A2 (en) * | 2023-06-20 | 2024-12-26 | Regeneron Pharmaceuticals, Inc. | Methods for purifying filled adeno-associated virus capsids |
| WO2025090962A1 (en) | 2023-10-25 | 2025-05-01 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| TW202540433A (zh) * | 2023-11-21 | 2025-10-16 | 美商再生元醫藥公司 | 藉由活體外接合產生共價表面修飾之腺相關病毒及共價表面修飾的腺相關病毒之純化 |
| EP4606892A1 (en) * | 2024-02-23 | 2025-08-27 | Merck Patent GmbH | Methods for the production of viral particles |
| WO2025226558A1 (en) * | 2024-04-22 | 2025-10-30 | Ultragenyx Pharmaceutical Inc. | Multi-step filtration and clarification methods for recombinant adeno-associated virus purification |
| WO2025228321A1 (en) * | 2024-04-29 | 2025-11-06 | Skyline Therapeutics (Shanghai) Co., Ltd. | METHOD OF SEPARATING RECOMBINANT ADENO-ASSOCIATED VIRUS (rAAV) PARTICLES |
Family Cites Families (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6989264B2 (en) | 1997-09-05 | 2006-01-24 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
| CA2379166C (en) | 1999-08-09 | 2013-03-26 | Targeted Genetics Corporation | Enhancement of expression of a single-stranded, heterologous nucleotide sequence from recombinant viral vectors by designing the sequence such that it forms instrastrand base pairs |
| JP2003518380A (ja) * | 1999-12-29 | 2003-06-10 | ジェンザイム・コーポレイション | 核酸および他の細胞性汚染混入物質からウイルスベクターを分離するための濾過助剤の使用方法 |
| WO2002000337A1 (en) | 2000-06-27 | 2002-01-03 | Bia Separations D.O.O. | A chromatography material and a process of manufacturing that material |
| US6593123B1 (en) | 2000-08-07 | 2003-07-15 | Avigen, Inc. | Large-scale recombinant adeno-associated virus (rAAV) production and purification |
| US6723551B2 (en) | 2001-11-09 | 2004-04-20 | The United States Of America As Represented By The Department Of Health And Human Services | Production of adeno-associated virus in insect cells |
| MX359371B (es) | 2001-11-13 | 2018-09-25 | Univ Pennsylvania | Un metodo para detectar y/o identificar secuencias del virus adeno-asociado y aislamiento de secuencias novedosas identificadas de ese modo. |
| PT1453547T (pt) | 2001-12-17 | 2016-12-28 | Univ Pennsylvania | Sequências do vírus adeno-associado (aav) do serotipo 8, vetores contendo as mesmas, e utilizações destas |
| US7261544B2 (en) | 2003-05-21 | 2007-08-28 | Genzyme Corporation | Methods for producing preparations of recombinant AAV virions substantially free of empty capsids |
| EP2345731B1 (en) | 2003-09-30 | 2015-10-21 | The Trustees of the University of Pennsylvania | Adeno-associated virus (AAV) clades, sequences, vectors containing same, and uses thereof |
| US7183969B2 (en) | 2004-12-22 | 2007-02-27 | Raytheon Company | System and technique for calibrating radar arrays |
| ES2525067T3 (es) | 2005-04-07 | 2014-12-17 | The Trustees Of The University Of Pennsylvania | Método de incremento de la función de un vector de AAV |
| JP4495210B2 (ja) | 2005-06-09 | 2010-06-30 | パナソニック株式会社 | 振幅誤差補償装置及び直交度誤差補償装置 |
| ES2751999T3 (es) | 2008-01-29 | 2020-04-02 | Applied Genetic Tech Corporation | Producción recombinante de virus adeno-asociados usando células BHK en suspensión |
| JP2011512156A (ja) | 2008-02-19 | 2011-04-21 | アムステルダム モレキュラー セラピューティクス ビー．ブイ． | 昆虫細胞におけるパルボウイルスｒｅｐ及びｃａｐタンパク質の発現の最適化 |
| EP2425000B1 (en) | 2009-04-30 | 2019-02-20 | The Trustees Of The University Of Pennsylvania | Compositions for targeting conducting airway cells comprising adeno-associated virus constructs |
| US8734809B2 (en) | 2009-05-28 | 2014-05-27 | University Of Massachusetts | AAV's and uses thereof |
| US8628966B2 (en) | 2010-04-30 | 2014-01-14 | City Of Hope | CD34-derived recombinant adeno-associated vectors for stem cell transduction and systemic therapeutic gene transfer |
| US8927514B2 (en) | 2010-04-30 | 2015-01-06 | City Of Hope | Recombinant adeno-associated vectors for targeted treatment |
| WO2012057363A1 (ja) | 2010-10-27 | 2012-05-03 | 学校法人自治医科大学 | 神経系細胞への遺伝子導入のためのアデノ随伴ウイルスビリオン |
| WO2012109570A1 (en) | 2011-02-10 | 2012-08-16 | The University Of North Carolina At Chapel Hill | Viral vectors with modified transduction profiles and methods of making and using the same |
| CN105755044A (zh) | 2011-04-22 | 2016-07-13 | 加利福尼亚大学董事会 | 具有变异衣壳的腺相关病毒病毒体及其使用方法 |
| ES2857773T5 (es) | 2011-08-24 | 2024-06-04 | Univ Leland Stanford Junior | Nuevas proteínas de la cápside de AAV para la transferencia de ácidos nucleicos |
| WO2013170078A1 (en) | 2012-05-09 | 2013-11-14 | Oregon Health & Science University | Adeno associated virus plasmids and vectors |
| CA2905952A1 (en) | 2013-03-13 | 2014-10-02 | The Children's Hospital Of Philadelphia | Adeno-associated virus vectors and methods of use thereof |
| MX388221B (es) | 2013-04-20 | 2025-03-19 | Res Institute At Nationwide Children´S Hospital | ADMINISTRACIÓN DE VIRUS ADENO-ASOCIADO RECOMBINANTE DE CONSTRUCCIONES DE POLINUCLÉOTIDOS U7snRNA DIRIGIDA AL EXÓN 2. |
| IL297919A (en) | 2013-07-22 | 2023-01-01 | Childrens Hospital Philadelphia | Variant aav and compositions, methods and uses for gene transfer to cells, organs and tissues |
| ES2739288T3 (es) | 2013-09-13 | 2020-01-30 | California Inst Of Techn | Recuperación selectiva |
| CN106232618A (zh) | 2013-10-11 | 2016-12-14 | 马萨诸塞眼科耳科诊所 | 预测祖先病毒序列的方法及其用途 |
| WO2015164757A1 (en) | 2014-04-25 | 2015-10-29 | Oregon Health & Science University | Methods of viral neutralizing antibody epitope mapping |
| EP3151866B1 (en) | 2014-06-09 | 2023-03-08 | Voyager Therapeutics, Inc. | Chimeric capsids |
| AU2015320694B2 (en) | 2014-09-24 | 2021-11-11 | City Of Hope | Adeno-associated virus vector variants for high efficiency genome editing and methods thereof |
| EP3054007A1 (en) * | 2015-02-09 | 2016-08-10 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step |
| JP6665466B2 (ja) | 2015-09-26 | 2020-03-13 | 日亜化学工業株式会社 | 半導体発光素子及びその製造方法 |
| WO2017070491A1 (en) | 2015-10-23 | 2017-04-27 | Applied Genetic Technologies Corporation | Ophthalmic formulations |
| ES2934848T3 (es) * | 2015-12-11 | 2023-02-27 | Univ Pennsylvania | Método de purificación escalable para AAV8 |
| US11028372B2 (en) | 2015-12-11 | 2021-06-08 | The Trustees Of The University Of Pennsylvania | Scalable purification method for AAVRH10 |
| WO2017100674A1 (en) * | 2015-12-11 | 2017-06-15 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav1 |
| EP3387138B1 (en) * | 2015-12-11 | 2022-01-26 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav9 |
| WO2017160704A1 (en) * | 2016-03-14 | 2017-09-21 | Perumala Corporation | Photovoltaic systems with intermittent and continuous recycling of light |
| WO2019178495A1 (en) | 2018-03-16 | 2019-09-19 | Biogen Ma Inc. | Methods for purifying recombinant adeno-associated viruses |
-
2019
- 2019-06-13 US US17/251,869 patent/US12070702B2/en active Active
- 2019-06-13 IL IL319002A patent/IL319002A/en unknown
- 2019-06-13 CN CN201980048303.1A patent/CN112469822A/zh active Pending
- 2019-06-13 JP JP2020568998A patent/JP7385603B2/ja active Active
- 2019-06-13 IL IL279193A patent/IL279193B2/en unknown
- 2019-06-13 AU AU2019285186A patent/AU2019285186B2/en active Active
- 2019-06-13 KR KR1020217000909A patent/KR102930150B1/ko active Active
- 2019-06-13 EP EP19748612.9A patent/EP3807405A2/en active Pending
- 2019-06-13 CA CA3102817A patent/CA3102817A1/en active Pending
- 2019-06-13 WO PCT/US2019/037013 patent/WO2019241535A2/en not_active Ceased
-
2024
- 2024-07-19 US US18/777,913 patent/US20250186904A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2021526829A5 (enExample) | ||
| US12351832B2 (en) | Recombinant adeno-associated virus particle purification with multiple-step anion exchange chromatography | |
| US20220364111A1 (en) | Methods for producing preparations of recombinant aav virions substantially free of empty capsids | |
| EP3256574B1 (en) | Recombinant adeno-associated virus particle purification comprising an affinity purification step | |
| JP2018510648A5 (enExample) | ||
| JP2018522529A5 (enExample) | ||
| JP2021514659A5 (enExample) | ||
| JP2017509632A5 (enExample) | ||
| JP2024525142A5 (enExample) | ||
| JP2024511409A5 (enExample) | ||
| CA2975569C (en) | Recombinant adeno-associated virus particle purification comprising an affinity purification step | |
| RU2025127724A (ru) | Молекула нуклеиновой кислоты, содержащая асимметрично модифицированный ITR для повышения уровня экспрессии встроенного гена, и ее применение | |
| CN119816351A (zh) | 用于分离完整的aav颗粒和空aav颗粒的方法 | |
| HK1227436A (en) | Recombinant adeno-associated virus particle purification with multiple-step anion exchange chromatography | |
| HK1227436A1 (en) | Recombinant adeno-associated virus particle purification with multiple-step anion exchange chromatography | |
| NZ761655A (en) | Cellular models of and therapies for ocular diseases | |
| HK1227435A (en) | Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step | |
| HK1227435A1 (en) | Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step | |
| NZ761655B2 (en) | Cellular models of and therapies for ocular diseases |