JP2021503003A - アミン保護された部分を含むヌクレオチド誘導体、並びに鋳型依存性及び鋳型非依存性酵素的核酸合成におけるそれらの使用 - Google Patents

アミン保護された部分を含むヌクレオチド誘導体、並びに鋳型依存性及び鋳型非依存性酵素的核酸合成におけるそれらの使用 Download PDF

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JP2021503003A
JP2021503003A JP2020544181A JP2020544181A JP2021503003A JP 2021503003 A JP2021503003 A JP 2021503003A JP 2020544181 A JP2020544181 A JP 2020544181A JP 2020544181 A JP2020544181 A JP 2020544181A JP 2021503003 A JP2021503003 A JP 2021503003A
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シー．チェン マイケル
シー．チェン マイケル
アール．ムシンロイ ゴードン
アール．ムシンロイ ゴードン
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ヌクレラ ヌクレイクス リミテッド
ヌクレラ ヌクレイクス リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
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    • C—CHEMISTRY; METALLURGY
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    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2020544181A 2017-11-14 2018-11-14 アミン保護された部分を含むヌクレオチド誘導体、並びに鋳型依存性及び鋳型非依存性酵素的核酸合成におけるそれらの使用 Pending JP2021503003A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1718804.6A GB201718804D0 (en) 2017-11-14 2017-11-14 Novel use
GB1718804.6 2017-11-14
PCT/GB2018/053305 WO2019097233A1 (en) 2017-11-14 2018-11-14 Nucleotide derivatives containing amine masked moieties and their use in a templated and non-templated enzymatic nucleic acid synthesis

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JP2021503003A true JP2021503003A (ja) 2021-02-04
JP2021503003A5 JP2021503003A5 (https=) 2021-12-16

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JP2020544181A Pending JP2021503003A (ja) 2017-11-14 2018-11-14 アミン保護された部分を含むヌクレオチド誘導体、並びに鋳型依存性及び鋳型非依存性酵素的核酸合成におけるそれらの使用

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US (1) US11505815B2 (https=)
EP (1) EP3710461A1 (https=)
JP (1) JP2021503003A (https=)
CN (1) CN111344296A (https=)
AU (1) AU2018366391A1 (https=)
CA (1) CA3082429A1 (https=)
GB (2) GB201718804D0 (https=)
WO (1) WO2019097233A1 (https=)

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Publication number Priority date Publication date Assignee Title
AU2019311199B2 (en) 2018-07-23 2024-12-19 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
SG11202107921SA (en) 2019-02-12 2021-08-30 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
WO2020165334A1 (en) * 2019-02-14 2020-08-20 Dna Script Process for preparing 3'-o-amino-ribonucleotide
GB201906772D0 (en) * 2019-05-14 2019-06-26 Nuclera Nucleics Ltd Nucleic acid polymer with amine-masked bases
EP4126895A1 (en) * 2020-03-30 2023-02-08 DNA Script Method for preparing 3'-o-amino-2'-deoxyribonucleoside-5'-triphosphates
EP4132941A2 (en) * 2020-04-06 2023-02-15 Nuclera Nucleics Ltd 3'-aminooxy-c5-substituted cytosine nucleotide derivatives and their use in a templated and non-templated enzymatic nucleic acid synthesis
GB202005043D0 (en) * 2020-04-06 2020-05-20 Nuclera Nucleics Ltd C5-modified Thymidines
US11850258B1 (en) * 2020-05-29 2023-12-26 Nilesh B. Karalkar Ribonucleoside derivatives with 3′-aminoxy groups
WO2024227846A2 (en) 2023-05-03 2024-11-07 Dna Script Reagent used in deprotection of 3'-o-amino polynucleotides
CN116949152B (zh) * 2023-06-16 2025-03-11 广州国家实验室 一种单碱基水平检测基因组DNA上N4-mC及5mC修饰的测序方法

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JPS62502338A (ja) * 1985-03-15 1987-09-10 アンチバイラルズ　インコーポレイテッド 立体規則性ポリヌクレオチド結合ポリマ−
JPS63275598A (ja) * 1987-04-10 1988-11-14 ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング デスアザプリン―ヌクレオシド―誘導体
JPH11512397A (ja) * 1995-09-07 1999-10-26 ユニバーシティー オブ ジョージア リサーチ ファウンデーション，インコーポレイテッド 治療用アジド化合物
JP2011520845A (ja) * 2008-05-15 2011-07-21 カソリック ユニヴェルシテイト ルーヴェン，ケー．ユー． ルーヴェン アール アンド ディー 抗癌剤の組合せによる治療
US8034923B1 (en) * 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
KR101093787B1 (ko) * 2011-06-10 2011-12-19 (주)비씨월드제약 신규한 뉴클레오시드 유도체를 포함하는 암 치료용 약학적 조성물
KR20120051328A (ko) * 2010-11-12 2012-05-22 (주)비씨월드제약 신규한 뉴클레오시드 유도체

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US4672111A (en) * 1985-04-23 1987-06-09 University Of Wyoming 5-azido-deoxyuridine compounds and method of synthesis of the same
IT1377936B1 (it) 2007-03-28 2010-07-30 Nucleotidi piridinici acitali, loro reparazione ed uso
US9309569B2 (en) * 2010-08-26 2016-04-12 Intelligent Bio-Systems, Inc. Methods and compositions for sequencing nucleic acid using charge
GB201502152D0 (en) * 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
WO2017087887A1 (en) * 2015-11-18 2017-05-26 The Trustees Of Columbia University In The City Of New York Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators

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JPS62502338A (ja) * 1985-03-15 1987-09-10 アンチバイラルズ　インコーポレイテッド 立体規則性ポリヌクレオチド結合ポリマ−
JPS63275598A (ja) * 1987-04-10 1988-11-14 ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング デスアザプリン―ヌクレオシド―誘導体
JPH11512397A (ja) * 1995-09-07 1999-10-26 ユニバーシティー オブ ジョージア リサーチ ファウンデーション，インコーポレイテッド 治療用アジド化合物
JP2011520845A (ja) * 2008-05-15 2011-07-21 カソリック ユニヴェルシテイト ルーヴェン，ケー．ユー． ルーヴェン アール アンド ディー 抗癌剤の組合せによる治療
US8034923B1 (en) * 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
KR20120051328A (ko) * 2010-11-12 2012-05-22 (주)비씨월드제약 신규한 뉴클레오시드 유도체
KR101093787B1 (ko) * 2011-06-10 2011-12-19 (주)비씨월드제약 신규한 뉴클레오시드 유도체를 포함하는 암 치료용 약학적 조성물

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EP3710461A1 (en) 2020-09-23
WO2019097233A1 (en) 2019-05-23
CA3082429A1 (en) 2019-05-23
GB201818605D0 (en) 2018-12-26
AU2018366391A1 (en) 2020-06-25
GB201718804D0 (en) 2017-12-27
GB2568397A (en) 2019-05-15
US11505815B2 (en) 2022-11-22
US20190144905A1 (en) 2019-05-16
CN111344296A (zh) 2020-06-26

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