CN111344296A - 含有胺掩蔽的部分的核苷酸衍生物及其在模板化和非模板化酶促核酸合成中的用途 - Google Patents

含有胺掩蔽的部分的核苷酸衍生物及其在模板化和非模板化酶促核酸合成中的用途 Download PDF

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CN111344296A
CN111344296A CN201880073408.8A CN201880073408A CN111344296A CN 111344296 A CN111344296 A CN 111344296A CN 201880073408 A CN201880073408 A CN 201880073408A CN 111344296 A CN111344296 A CN 111344296A
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nucleic acid
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M·C·陈
G·R·米钦罗伊
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Nuclera Ltd
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Nuclera Nucleics Ltd
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    • C07—ORGANIC CHEMISTRY
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    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
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    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
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  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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CN201880073408.8A 2017-11-14 2018-11-14 含有胺掩蔽的部分的核苷酸衍生物及其在模板化和非模板化酶促核酸合成中的用途 Pending CN111344296A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1718804.6A GB201718804D0 (en) 2017-11-14 2017-11-14 Novel use
GB1718804.6 2017-11-14
PCT/GB2018/053305 WO2019097233A1 (en) 2017-11-14 2018-11-14 Nucleotide derivatives containing amine masked moieties and their use in a templated and non-templated enzymatic nucleic acid synthesis

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CN111344296A true CN111344296A (zh) 2020-06-26

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CN201880073408.8A Pending CN111344296A (zh) 2017-11-14 2018-11-14 含有胺掩蔽的部分的核苷酸衍生物及其在模板化和非模板化酶促核酸合成中的用途

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US (1) US11505815B2 (https=)
EP (1) EP3710461A1 (https=)
JP (1) JP2021503003A (https=)
CN (1) CN111344296A (https=)
AU (1) AU2018366391A1 (https=)
CA (1) CA3082429A1 (https=)
GB (2) GB201718804D0 (https=)
WO (1) WO2019097233A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116949152A (zh) * 2023-06-16 2023-10-27 广州国家实验室 一种单碱基水平检测基因组DNA上N4-mC及5mC修饰的测序方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2019311199B2 (en) 2018-07-23 2024-12-19 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
SG11202107921SA (en) 2019-02-12 2021-08-30 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
WO2020165334A1 (en) * 2019-02-14 2020-08-20 Dna Script Process for preparing 3'-o-amino-ribonucleotide
GB201906772D0 (en) * 2019-05-14 2019-06-26 Nuclera Nucleics Ltd Nucleic acid polymer with amine-masked bases
EP4126895A1 (en) * 2020-03-30 2023-02-08 DNA Script Method for preparing 3'-o-amino-2'-deoxyribonucleoside-5'-triphosphates
EP4132941A2 (en) * 2020-04-06 2023-02-15 Nuclera Nucleics Ltd 3'-aminooxy-c5-substituted cytosine nucleotide derivatives and their use in a templated and non-templated enzymatic nucleic acid synthesis
GB202005043D0 (en) * 2020-04-06 2020-05-20 Nuclera Nucleics Ltd C5-modified Thymidines
US11850258B1 (en) * 2020-05-29 2023-12-26 Nilesh B. Karalkar Ribonucleoside derivatives with 3′-aminoxy groups
WO2024227846A2 (en) 2023-05-03 2024-11-07 Dna Script Reagent used in deprotection of 3'-o-amino polynucleotides

Citations (4)

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US20010036930A1 (en) * 1995-09-07 2001-11-01 University Of Georgia Research Foundation, Inc. Therapeutic azide compounds
US20110065663A1 (en) * 2008-05-15 2011-03-17 Katholieke Universiteit Leuven, K.U. Leuven R&D Anti-cancer combination therapy
US8034923B1 (en) * 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
WO2016128731A1 (en) * 2015-02-10 2016-08-18 Nuclera Nucleics Ltd Novel use

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JP3022967B2 (ja) * 1985-03-15 2000-03-21 アンチバイラルズ インコーポレイテッド 立体規則性ポリヌクレオチド結合ポリマー
US4672111A (en) * 1985-04-23 1987-06-09 University Of Wyoming 5-azido-deoxyuridine compounds and method of synthesis of the same
DE3739366A1 (de) * 1987-04-10 1988-10-27 Boehringer Mannheim Gmbh Desaza-purin-nucleosid-derivate, verfahren zu deren herstellung sowie deren verwendung bei der nucleinsaeure-sequenzierung sowie als antivirale mittel
IT1377936B1 (it) 2007-03-28 2010-07-30 Nucleotidi piridinici acitali, loro reparazione ed uso
US9309569B2 (en) * 2010-08-26 2016-04-12 Intelligent Bio-Systems, Inc. Methods and compositions for sequencing nucleic acid using charge
KR101794970B1 (ko) * 2010-11-12 2017-11-07 (주)비씨월드제약 신규한 뉴클레오시드 유도체
KR101093787B1 (ko) * 2011-06-10 2011-12-19 (주)비씨월드제약 신규한 뉴클레오시드 유도체를 포함하는 암 치료용 약학적 조성물
WO2017087887A1 (en) * 2015-11-18 2017-05-26 The Trustees Of Columbia University In The City Of New York Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators

Patent Citations (4)

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US20010036930A1 (en) * 1995-09-07 2001-11-01 University Of Georgia Research Foundation, Inc. Therapeutic azide compounds
US20110065663A1 (en) * 2008-05-15 2011-03-17 Katholieke Universiteit Leuven, K.U. Leuven R&D Anti-cancer combination therapy
US8034923B1 (en) * 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
WO2016128731A1 (en) * 2015-02-10 2016-08-18 Nuclera Nucleics Ltd Novel use

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HUTTER, DANIEL ETC.: "Labeled Nucleoside Triphosphates with Reversibly Terminating Aminoalkoxyl Groups" *
PANJA, SUBIR ETC.: "Highly chemoselective reduction of azides to amines by Fe(0) nanoparticles in water at room temperatur" *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116949152A (zh) * 2023-06-16 2023-10-27 广州国家实验室 一种单碱基水平检测基因组DNA上N4-mC及5mC修饰的测序方法

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EP3710461A1 (en) 2020-09-23
WO2019097233A1 (en) 2019-05-23
CA3082429A1 (en) 2019-05-23
GB201818605D0 (en) 2018-12-26
AU2018366391A1 (en) 2020-06-25
GB201718804D0 (en) 2017-12-27
GB2568397A (en) 2019-05-15
US11505815B2 (en) 2022-11-22
US20190144905A1 (en) 2019-05-16
JP2021503003A (ja) 2021-02-04

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Application publication date: 20200626