JP2021010365A - Campanula plants, production method of campanula plants, screening method of campanula plants, and method for changing day-length on bolting in campanula plants - Google Patents

Abstract

To provide Campanula plants capable of bolting even under non-long day conditions.SOLUTION: The present invention discloses a Campanula plant bolting in cultivation under non-long day conditions, a production methods of Campanula plants, a screening method of Campanula plants, and a method for changing the day-length on bolting in Campanula plants.SELECTED DRAWING: Figure 4

Description

The present invention relates to a campanula plant, a method for producing a campanula plant, a method for screening a campanula plant, and a method for changing a drawing day length in a campanula plant.

Campanula medium species plants (hereinafter also referred to as "medium" or "medium plants") are ornamental plants with wind chimes-like petals.

Medium is a green vernalization type plant that requires low temperature and long day conditions for flower bud formation and extraction. For this reason, medium is a typical biennial plant that is generally sown and raised in spring, planted in autumn and overwintered, and blooms the following year (Non-Patent Document 1).

Breeding has been carried out in the medium, and non-low temperature demanding varieties have been developed for flower bud formation and drawing.

In addition, Sakamoto, "Additional Agricultural Technology System Flower Edition 8 1.2 Years Grass", June 30, 1994, Rural Culture Association Japan, pp. 33-39

Even in low temperature non-requiring medium varieties, long-day cultivation is required for flower bud formation and extraction. For this reason, when the medium is extracted under short-day conditions such as winter, a long-day treatment of 16 hours is performed using an electric lighting facility, which causes a problem that labor is required.

Therefore, an object of the present invention is to provide a Campanula plant (hereinafter, also referred to as "Campanula plant") that can be extracted even under non-long-day conditions.

In order to achieve the above object, the campanula plant of the present invention is drawn in cultivation under non-long day conditions.

The method for producing a campanula plant of the present invention (hereinafter, also referred to as "manufacturing method") includes a crossing step of crossing a first campanula plant and a second campanula plant.
The first campanula plant is the campanula plant of the present invention.

The method for screening a non-long-day campanula plant of the present invention (hereinafter, also referred to as “screening method”) includes a selection step of cultivating a test campanula plant under non-long-day conditions and selecting a extracted campanula plant. ..

The method for changing the drawing day length in the campanula plant of the present invention (hereinafter, also referred to as "change method") is to cultivate under non-long day conditions by crossing the first campanula plant and the second campanula plant. Including the breeding process to breed progeny lines that can be extracted in
The first campanula plant is the campanula plant of the present invention.

The campanula plant of the present invention can be extracted even under non-long day conditions. Therefore, according to the campanula plant of the present invention, the campanula plant such as medium can be extracted even under short-day conditions such as winter without using an electric lighting facility. Therefore, according to the present invention, for example, labor and cost in cultivating a campanula plant can be reduced.

FIG. 1 is a schematic cross-sectional view of a Campanula plant. FIG. 2 is a system diagram relating to breeding of the deposited line in Example 1 of the present application. FIG. 3 is a photograph showing the Campanula medium plant in Example 1 of the present application. FIG. 4 is a photograph showing the Campanula medium plant in Example 1 of the present application. FIG. 5 is a photograph showing the Campanula medium plant in Example 1 of the present application. FIG. 6 is a photograph showing the Campanula medium plant in Example 1 of the present application. FIG. 7 is a photograph showing the Campanula medium plant in Example 1 of the present application. FIG. 8 is a photograph showing the Campanula medium plant in Example 1 of the present application. FIG. 9 is a photograph showing the Campanula medium plant in Example 1 of the present application. FIG. 10 is a photograph showing the Campanula medium plant in Example 2 of the present application. FIG. 11 is a photograph showing the Campanula medium plant in Example 2 of the present application. FIG. 12 is a photograph showing the Campanula medium plant in Example 3 of the present application.

1. 1. Campanula plant The campanula plant of the present invention is drawn in cultivation under non-long day conditions. The campanula plant of the present invention is characterized by being drawn in cultivation under non-long-day conditions, and other configurations and conditions are not particularly limited. As described above, the campanula plant of the present invention can be extracted even under non-long-day conditions (hereinafter, also referred to as "non-long-day"). Therefore, according to the campanula plant of the present invention, the campanula plant can be extracted even under short-day conditions such as winter without using an electric lighting facility. Further, in the campanula plant of the present invention, for example, a characteristic that can be extracted in cultivation under non-long-day conditions is a genetic characteristic. Therefore, by using the campanula plant of the present invention, more specifically, by crossing with the campanula plant of the present invention, for example, other campanula plants can be extracted in cultivation under non-long-day conditions. Possible properties can be imparted.

In the present invention, the "Campanula plant" is a plant classified into the genus Campanula. The Campanula plant of the genus, for example, Campanula medium species (Campanula medium), Campanula glomerata species, Campanula Persicifolia species, may be mentioned plants such as Campanula punctata species, preferably, Campanula medium species. The Campanula plant may be, for example, a closely related species or a hybrid with a wild species.

In the present invention, "plant" may mean either a plant individual indicating the whole plant or a part of the plant individual. The portion of the individual plant may be, for example, an organ, a tissue, a cell, a vegetative propagule, or the like. Examples of the organ include petals, corolla, flowers, leaves, seeds, fruits, stems, roots and the like. The tissue is, for example, a part of the organ. The plant part may be, for example, one type of organ, tissue and / or cell, or two or more types of organ, tissue and / or cell.

In the present invention, the "non-long-day condition" means a condition that is not a long-day condition, and can be, for example, a short-day condition. The long-day condition means that, for example, in the light and dark periods of the day, the length of the light period is longer than the length of the dark period. Therefore, under the non-long day condition, for example, in the light period and the dark period of the day, the length of the light period can be shorter than the length of the dark period. The day means one day and night, and specifically, it is about 24 hours.

The "dark period" means, for example, the cultivation time under darkness (about 0 lux) or under the condition that there is light in which drawing or flowering is not induced (condition in which light interruption does not occur). The condition is, for example, 20 lux or less, preferably 10 lux or less.

The light period means a cultivation time under a condition in which light having an illuminance (amount of light) capable of inducing drawing or flowering is present. The type of light source of light emitted to the campanula plant in the light period is not particularly limited, and examples thereof include sunlight, illumination light, or a combination thereof. The sunlight is, for example, light derived from the sun. The illumination light is, for example, light derived from a lighting device. Examples of the lighting device include incandescent lamps, fluorescent lamps, LEDs (light emitting diodes) and the like. The illuminance of light in the light period is, for example, more than 20 lux, preferably 50 lux or more, 100 lux or more, or 150 lux or more. The range of light illuminance in the light period is, for example, 50 to 200 lux, or 50 to 150 lux.

In the non-long day condition, the length of the dark period exceeds, for example, 12 hours, preferably 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, 18 hours, 19 hours, 20 hours, 21 hours. 22 hours, or 23 hours or more. In the non-long day condition, the length of the dark period is, for example, 13 to 20 hours or 14 to 20 hours, preferably 16 to 20 hours. The length of the dark period is, for example, the length of the dark period in one day under the long-day condition when one day is 24 hours. The dark period may be a discontinuous dark period or a continuous dark period, but is preferably a continuous dark period. When the dark period is a discontinuous dark period, the length of the dark period in the day is the total time of the lengths of each dark period in the day.

In the non-long day condition, the length of the light period is, for example, 12 hours or less or less than 12 hours, preferably 11 hours, 10 hours, 9 hours, 8 hours, 7 hours, 6 hours, 5 hours, 4 hours. Hours, 3 hours, 2 hours, or 1 hour or less. In the non-long day condition, the range of the light period is, for example, 4 to 11 hours or 4 to 10 hours, preferably 4 to 8 hours. The length of the light period is, for example, the length of the light period in one day under the long-day condition when one day is 24 hours. The light period may be a discontinuous light period or a continuous light period, but is preferably a continuous light period. By setting the length of the light period to 4 hours or more, for example, the flower stalk of the campanula plant of the present invention can be further elongated.

The cultivation conditions of the Campanula plant under the non-long-day conditions are not particularly limited, and general cultivation conditions of the Campanula plant can be adopted. The cultivating place of the campanula plant may be, for example, an open field or a greenhouse such as a house, but is preferably a greenhouse. The cultivation temperature of the Campanula plant is, for example, 3 to 30 ° C, 8 to 30 ° C, or 12 to 30 ° C. When the Campanula plant exhibits the non-low temperature requirement described below, the cultivation temperature is, for example, 8 to 30 ° C or 12 to 30 ° C.

In the present invention, "drawing" means that, for example, in a rosette-type campanula plant, flower stalk elongation occurs. In the campanula plant of the present invention, for example, as shown in FIG. 1, the length (T) from the bottom (ground surface) to the tip of the stem (tip of the apical bud) is 5 cm or more. Preferably, when the length is 10 cm or more, it can be determined that the flower stalk has been elongated. Also, in Campanula plants, flowering occurs after drawing. Therefore, in the present invention, the "drawing" also includes, for example, the meaning of flowering or a state in which flowering is possible.

The campanula plant of the present invention can be drawn, for example, under a non-drawing condition (non-drawing condition) in addition to a drawing condition for a campanula plant variety (control variety) that requires a long-day condition for drawing. Is. Examples of the control varieties include the Campanula Champion Series such as Campanula Champion Purple (ver.2) or the Chime Series (manufactured by Sakata Seed Corporation), and the Campanula Campana Series (manufactured by Miyoshi Co., Ltd.). Examples of the non-drawing condition include the non-long day condition described above, and as a specific example, the test condition described later can be referred to.

In the present invention, the fact that the plant can be extracted under the non-long-day conditions means that, for example, when the Campanula plant to be tested is cultivated under the following cultivation conditions (test conditions), there is an individual to be extracted. The extracted individual is, for example, 85% or more, 90% or more, or 95% or more, preferably 96, 97, 98, or 99% or more of all the individuals of the test campanula plant. , More preferably about 100%. The number of the Campanula plants to be tested is, for example, 5 to 100 individuals and 48 to 100 individuals.
(Cultivation conditions)
Cultivation place: House cultivation Cultivation temperature: 3 to 30 ° C
Photoperiod: Repeating the following light and dark periods Light period: 4 to 8 hours Dark period: 16 to 20 hours Cultivation period: 180 to 200 days Cultivation method:
After sowing the seeds of the Campanula plant to be tested on the surface of the potting soil, the plants are cultivated under the conditions of 15 to 18 ° C. (daily fluctuation) without covering the soil. As the culture soil, for example, commercially available culture soil can be used. The potting soil is preferably weakly acidic. After germination of the seeds, seedlings are raised under the conditions of 12 to 30 ° C. (daily fluctuation). After raising the seedlings, the seedlings are planted and cultivated under the conditions of 12 to 30 ° C. (daily fluctuation). When the test campanula plant requires low temperature, it is cultivated for 30 days under the condition of 4 ° C. after maturation of the test campanula plant (after the formation of the rosette), and again under the condition of 12 to 30 ° C. (daily variation). Cultivate in. On the other hand, when the test campanula plant has a non-low temperature requirement, it is cultivated under the condition of 12 to 30 ° C. (daily fluctuation).

In the campanula plant of the present invention, characteristics or characteristics other than those that can be extracted under non-long-day conditions, such as low temperature requirement or non-low temperature requirement, trait characteristics, ecological characteristics, etc., are not particularly limited. The campanula plant of the present invention may be non-cold demand, for example, in flower bud formation and drawing. The low temperature requirement means that, for example, in a mature state of the target campanula plant, if the condition of 4 ° C. continues for 30 days, it can be drawn or flowered depending on the long-day condition or the like.

Examples of the campanula plant of the present invention include medium seed plants (deposited lines) deposited under accession number FERM BP-22375 or progeny lines. The deposited line exhibits non-low temperature requirements, for example in flower bud formation and drawing. The progeny line can be drawn, for example, under non-long day conditions. The deposit information is shown below.
Type of deposit: International deposit Institution Name: Independent Administrative Institution Product Evaluation Technology Infrastructure Organization Patent Organism Deposit Center Address: Japan 〒292-0818 2-5-8 Kazusakamatari, Kisarazu-shi, Chiba Deposit number: FERM BP- 22375
Display for identification: Takii 11
Receipt date: June 25, 2019

The deposited line shows the following characteristics (1) to (4), for example, when cultivated and compared with the control variety under the same conditions. The control cultivar is, for example, a control cultivar having a similar petal color, and a specific example thereof is Campanula Champion Purple.
(1) Flowering is earlier than that of the control variety (2) The petal color is darker than that of the control variety (3) The total length of the rooted leaves (rosette leaves) is shorter than that of the control variety (3) 4) Compared with the control variety, the stem leaf is shorter than the total length of the stem leaf at the corresponding position.

The base sequence of the whole genomic DNA of the progeny line is, for example, 25, 30, 35, 35, 40, 45, 50, 55, 60, 65, 70, based on the base sequence of the whole genomic DNA of the deposit line. It is 75, or 80% or more, preferably 85, 90, 95, 96, 97, 98, or 99% or more identity. The identity can be calculated, for example, for each chromosome by using the default parameters of the nucleotide sequence of the whole genomic DNA of the deposit line and the nucleotide sequence of the whole genomic DNA of the progeny line using FASTA or BLAST. The progeny line preferably retains a locus in the genome of the deposited line that is linked to a property that can be extracted under the non-long-day condition. The locus can be identified, for example, by quantitative trait locus (QTL) analysis.

In the present invention, the progeny line may be, for example, a self-fertilized progeny or a backcross progeny.

2. 2. Method for Producing Campanula Plant As described above, the method for producing a campanula plant of the present invention includes a crossing step of crossing a first campanula plant and a second campanula plant, and the first campanula plant is described above. It is a campanula plant of the present invention. The production method of the present invention is characterized in that the campanula plant of the present invention is used for at least one of the parents in the crossing step, and other steps and conditions are not particularly limited. According to the production method of the present invention, a campanula plant that can be extracted without using an electric lighting facility can be produced even under short-day conditions such as winter. Therefore, the production method of the present invention can be suitably used for breeding new varieties of non-long-day campanula plants, for example. Therefore, the production method of the present invention can also be said to be, for example, a method for growing or breeding a non-long-day campanula plant. The production method of the present invention can be referred to the description of the campanula plant of the present invention.

In the present invention, the cross between the first campanula plant (first parent line) and the second campanula plant (second parent line) may be, for example, crossing between the same individuals (regular self-pollination). However, it may be a cross between the same cloned individuals or between individuals of a line maintained in an inbred strain (quasi-self-pollination), or a cross between different individuals (cross-pollination). In the case of the self-pollination, one of the first parent line and the second parent line is, for example, a female organ in the same individual and the other is pollen in the same individual. The quasi-self-pollination may be, for example, the case where the S genotype regarding self-incompatibility is the same, and the cross-pollination may be, for example, the case where the S genotype regarding self-incompatibility is different. In the crossing of Campanula plants, it is preferable that the first parent line and the second parent line have different S genotypes, and the cross is cross-pollination.

In the present invention, the first parent line is the campanula plant of the present invention, for example, a medium seed plant or a progeny line deposited under the above-mentioned accession number FERM BP-22375.

The second parent line is not particularly limited, and any campanula plant can be used. The second parent line may be, for example, a campanula plant of the same species taxonomically as the first parent line, or a campanula plant of a different species. The second parent line may be, for example, a non-long-day campanula plant or a long-day campanula plant. In addition, the second parent line may have other characteristics or characteristics. The other features include, for example, non-low temperature requirement in flower bud formation and drawing, early growth, low temperature elongation, leaflet property and the like.

The production method of the present invention may include, for example, after the crossing step, a growing step of growing a progeny line obtained in the crossing step. Examples of the growing conditions in the growing step include general growing conditions for Campanula plants.

The production method of the present invention may include, for example, a selection step of cultivating the obtained progeny line under non-long-day conditions and selecting the extracted Campanula plant. In the selection step, the selection of the campanula plant may be carried out, for example, by cultivating the campanula plant under the above-mentioned non-long-day conditions or test conditions, and selecting the campanula plant which has been drawn and / or flowered. It may be carried out by cultivating under the condition that the control variety is not drawn, and selecting the campanula plants that are drawn and / or flowered.

The selection step can be carried out, for example, by selecting a campanula plant that has been drawn and / or is in bloom by the 180th to 220th days, with the sowing date as the 0th day under the above cultivation conditions.

In the selection step, for example, selection may be further performed according to other characteristics or characteristics. The characteristics or characteristics may be, for example, the same as the selection of general Campanula plants, lines, varieties, etc. Specific examples include non-low temperature requirement, early growth, low temperature elongation, leaflet property and the like. can give.

The campanula plant of the present invention can be obtained, for example, by the production method of the present invention.

3. 3. Campanula Plant Screening Method As described above, the non-long-day Campanula plant screening method of the present invention includes a selection step of cultivating a test campanula plant under non-long-day conditions and selecting a extracted campanula plant. .. The screening method of the present invention is characterized in that a campanula plant cultivated under the non-long-day conditions and extracted is selected, and other steps and conditions are not particularly limited. According to the screening method of the present invention, non-long-day campanula plants can be screened. The screening method of the present invention can be referred to the description of the campanula plant and the production method of the present invention.

The test campanula plant is not particularly limited and may be any campanula plant, and specific examples thereof include the campanula plant of the present invention or a progeny line of the campanula plant of the present invention. The Campanula plant to be tested is preferably a medium seed plant or a progeny strain deposited under the accession number FERM BP-22375.

The selection step can be carried out in the same manner as described in the selection step in the production method of the present invention.

4. Method of changing the lottery day length in the campanula plant The method of changing the lottery day length in the campanula plant of the present invention is non-method by crossing the first campanula plant and the second campanula plant as described above. The first Campanula plant is the Campanula plant of the present invention, which comprises a breeding step of breeding a progeny line that can be extracted in long-day cultivation. According to the modification method of the present invention, the day length condition required for the campanula plant to be drawn can be modified. The modification method of the present invention can also be said to be, for example, a non-long-day imparting method. As the modification method of the present invention, the description of the campanula plant, the production method, and the screening method of the present invention can be incorporated.

The "drawing day length" means, for example, a day length condition required for a Campanula plant to be drawn or flowered. In the changing method of the present invention, the change of the drawing day length may be, for example, shortening of the drawing day length or extension of the drawing day length. The change in the drawing day length can be evaluated, for example, by determining whether or not the drawing day length has changed significantly as compared with the drawing day length of the reference campanula plant (reference plant). .. Examples of the reference plant include the first campanula plant and / or the second campanula plant, and the second campanula plant is preferable. Specifically, the change in the drawing day length is, for example, until the campanula plant for evaluating the change in the drawing day length and the reference plant are cultivated under the same conditions and drawn based on the sowing date. It can be carried out by comparing the day lengths.

For the crossing in the breeding step, the description of the crossing step in the production method of the present invention can be incorporated. Then, by obtaining the obtained seeds after the crossing, it is possible to obtain a progeny line that can be extracted in cultivation under non-long-day conditions.

In the breeding step, the drawing day lengths in the first parent line and the second parent line may be the same or different. The first parent line is preferably a medium seed plant or a progeny line deposited under the accession number FERM BP-22375.

Hereinafter, the present invention will be described in detail with reference to Examples, but the present invention is not limited to the embodiments described in the Examples.

[Example 1]
We bred a new non-long-day Campanula medium plant and confirmed that it can be extracted under non-long-day conditions.

According to the phylogenetic diagram of FIG. 2, a new non-long-day Campanula medium plant, a deposited line, was bred. Specifically, the deposited strain was bred as follows. Unless otherwise specified, breeding was carried out at the Nagano Research Farm Fujimi Test Site (Fujimi Town, Suwa District, Nagano Prefecture) of Takii & Co., Ltd.

(1) Breeding of deposited lines First, medium plants (19 lines) and medium plants (129 lines) owned by Takii seedlings were sown and raised in October 2011. Next, the obtained seedlings were planted in pots in December 2011 and overwintered by house cultivation. Then, in February 2012 (8 hours of day length (8 hours of light period, non-long day condition)), 129-1 lines, which are individuals that are drawn or bloom, were selected from 129 lines, and 1291 lines were selected. Seeds of 316 progeny lines crossed with 19 lines were obtained. Further, the seeds of the self-fertilized progeny (301 line) were obtained in the same manner except that the other medium plant (1 line) owned by Takii seedlings was self-fertilized instead of the 129 line.

Next, 301 lines and 316 lines of medium plants were sown and raised in October 2012. Furthermore, the obtained 301 and 316 seedlings were planted in pots in December 2012 and overwintered in the house. Then, the 316 system and the 301 system were set to non-low temperature conditions by heating the inside of the house to 12 ° C. from January 2013. In addition, the plants were cultivated without being illuminated by an electric lighting device (8 hours a day (8 hours in the light period, non-long day conditions)). Further, for the 316 lines, individuals drawn or flowering in February 2013 were selected (selected 316 lines). The 301 line of medium plants was cultivated and flowered in the same manner as the 316 line. Then, the selected 316 lines and 301 lines were crossed to obtain 51 lines of seeds. In addition, seeds of the self-fertilized progeny (71 lines) were obtained in the same manner except that the 301 lines were self-fertilized.

The 51 lines and 71 lines were sown and raised in October 2013. Next, the obtained 51 strains of seedlings were planted in pots in December 2013 and overwintered in the house. Furthermore, for 51 systems, the inside of the house was heated to 12 ° C. from January 2014 to set non-low temperature conditions. In addition, the plants were cultivated without being illuminated by an electric lighting device (8 hours a day (8 hours in the light period, non-long day conditions)). Then, in February 2014, individuals that were drawn or bloomed were selected (51 lines selected). The 71 medium plants were cultivated and flowered in the same manner as the 19 plants. The selected 51 lines and 71 lines were crossed to obtain seeds of the crossed progeny (105 lines).

Next, 105 strains of medium plants were sown and raised in October 2014. Then, the obtained seedlings were planted in pots in December 2014 and overwintered in the house. Furthermore, about 105 lines, the inside of the house was heated to 12 ° C. from January 2015 to set non-low temperature conditions. It was cultivated without being illuminated by an electric lighting device (8 hours a day (8 hours in the light period, non-long day conditions)). Then, in February 2015, the seeds of the self-fertilized progeny (85 lines) were obtained by selecting the individuals that were drawn or flowered and self-fertilizing them. Then, from October 2015, seeds of the self-fertilized progeny (48 lines) were obtained in the same manner except that 85 lines were used instead of 105 lines. Furthermore, seeds of the self-fertilized progeny (84 lines, deposited line) were obtained in the same manner except that 48 lines were used instead of 105 lines from October 2016.

(2) Confirmation of non-long-day nature of the deposited line The deposited line was sown and raised on October 6, 2017. Then, the obtained seedlings were planted in pots on December 15, 2017, and overwintered in the house. Next, the deposit system was set to non-low temperature conditions by heating the inside of the house to 12 ° C. from December 15, 2017. In addition, the deposited system was cultivated by dividing it into a group that was subjected to the lighting treatment by the lighting device and a group that was not subjected to the lighting treatment. For the group to be subjected to the lighting treatment, the lighting treatment was carried out for 16 hours per day using an electric lighting device (16 hours in the light period, long day conditions). On the other hand, in the group not subjected to the lighting treatment, the group having a natural day length (9 to 10 hours day length, 9 to 10 hours in the light period, non-long day condition A) was irradiated with natural light for only 8 hours in a day. After that, the group that was completely shaded (8 hours day length, 8 hours light period, non-long day condition B) was irradiated with natural light for only 7 hours a day, and then the group that was completely shaded (7 hours day length, light period 7). Time, non-long day condition C), the group that was irradiated with natural light for only 6 hours a day and then completely shaded (6 hours day length, 6 hours in the light period, non-long day condition D), only 5 hours in a day A group that was irradiated with natural light and then completely shaded (5 hours day length, 5 hours light period, non-long day condition E), and then was irradiated with natural light only for 4 hours in a day, and then completely shaded group (4 hours day). It was divided into long, 4 hours tomorrow, and non-long day condition F). In addition, each group was 48 individuals. Then, on April 14, 2018, each group was photographed. In addition, the controls were photographed in the same manner except that long-day Champion Purple (manufactured by Sakata Seed Corporation) was used for drawing and flowering. These results are shown in FIGS. 3-9.

FIGS. 3 to 9 are photographs showing the deposited strains and champion purple cultivated under each day length condition. In FIGS. 3 to 9, from the top, each photograph is a photograph of all 48 strains, a photograph of three representative strains from an oblique direction, and a photograph of three representative strains from a side direction. As shown in FIG. 3, under long-day conditions, all individuals (48 individuals) of the deposit line and Champion Purple flowered. The flowering start date of the deposited strain was April 2, 2018, and the flowering start date of Champion Purple was April 5. On the other hand, as shown in FIGS. 4 to 9, in the non-long-day condition, in the deposited line, flower stalk elongation, that is, drawing occurs in all the individuals, whereas in Champion Purple, the flower stalk in all the individuals. No elongation was seen, i.e. no drawing occurred. In addition, in FIG. 9, as shown by an arrow, in the deposit system, a lottery was observed even in a very short light period of non-long day condition F. From these results, it was found that the newly bred deposit line was non-long-day in terms of drawing or flowering. In addition, the deposited line was drawn and flowered even under non-low temperature conditions, indicating that it is non-low temperature required for drawing or flowering.

[Example 2]
It was confirmed that non-long-dayity is an inherited characteristic in novel non-long-day Campanula medium plants.

The deposited line and champion purple were raised in the same manner as in Example 1 (2) above. Then, the seeds of the F1 line were obtained by crossing the deposited line with the champion purple.

The deposited line and F1 line were sown and raised on September 15, 2018. Then, the obtained seedlings were planted in pots on November 15, 2018, and overwintered in the house. Further, after planting the pot, the inside of the house was heated to 12 ° C. to set a non-low temperature condition. Further, natural light was irradiated for only 6 hours a day, and then completely shaded (6 hours day length, 6 hours light period, non-long day condition D). In addition, each group was 48 individuals. Then, on March 25, 2019, each system was photographed. The controls were photographed in the same manner except that long-day champion purple or campana (manufactured by Miyoshi Co., Ltd.) was used for drawing and flowering. These results are shown in FIGS. 10 and 11.

10 and 11 are photographs showing the deposit line or F1 line and the champion purple and campana. In FIGS. 10 and 11, each photograph is a photograph of all 48 strains and three representative strains from the diagonal direction from the top. As shown in FIGS. 10 and 11, under non-long-day conditions, in the deposited line, flower stalk elongation, that is, drawing occurs in all individuals, whereas in Champion Purple and Campana, flower stalks occur in all individuals. No elongation was seen, i.e. no drawing occurred. In addition, in the F1 strain, which is the progeny of the cross between the deposited strain and the champion purple, flower stalk elongation, that is, drawing, occurred in all the individuals. From these results, it was found that the non-long-day trait of the newly bred deposit line is a hereditary trait and can be introduced into other campanula plants by crossing. In addition, since the trait is expressed in the F1 line, it was found that the trait is dominantly inherited.

[Example 3]
It was confirmed that non-long-dayity is an inherited characteristic in novel non-long-day Campanula medium plants.

The F1 line and Champion Purple of Example 2 were raised in the same manner as in Example 1 (2). Then, the seeds of the F2 strain were obtained by crossing the F1 strain with the champion purple.

The deposited line and F2 line were sown and raised on October 1, 2019. Then, the obtained seedlings were planted in pots on December 5, 2019, and overwintered in the house. Further, after planting the pot, the inside of the house was heated to 12 ° C. to set a non-low temperature condition. Further, natural light was irradiated for only 6 hours a day, and then completely shaded (6 hours day length, 6 hours light period, non-long day condition D). The deposit line was 100 individuals, and the F2 line was 200 individuals. Then, on March 30, 2020, each system was photographed. In addition, for each line, the percentage of individuals with lottery was calculated as the lottery rate. In addition, the controls were photographed in the same manner, except that long-day champion purple (100 individuals) was used for drawing and flowering, and the drawing rate was calculated. These results are shown in FIG.

FIG. 12 is a photograph showing a deposit system or F2 system and a champion purple. As shown in FIG. 12, under non-long-day conditions, 97% of individuals in the deposited line had flower stalk elongation, that is, drawing, whereas Champion Purple had flower stalk elongation in all individuals. Was not seen, that is, no drawing had occurred. In addition, in the F2 strain, which is the progeny of the cross between the F1 strain and Champion Purple, 21% of the individuals had flower stalk elongation, that is, drawing. From the results of Example 2 above, the non-long-day trait possessed by the deposited line was presumed to be a dominantly inherited trait, but in this case, it is assumed that about 50% of the F2 line expresses the trait. To. On the other hand, if the trait is controlled by two genes and the trait is expressed when the two genes are combined, in the F1 line, almost all individuals express the trait, and in the F2 line, the trait is expressed. It is estimated that about 25% will express the trait. Therefore, Example 2 and the above results meet the latter assumption. From these results, it was found that the non-long-day trait of the newly bred deposit line is presumed to be a characteristic expressed by the combination of the two genes and can be introduced into other Campanula plants by crossing.

Although the present invention has been described above with reference to the embodiments and examples, the present invention is not limited to the above embodiments and examples. Various changes that can be understood by those skilled in the art can be made to the structure and details of the present invention within the scope of the present invention.

This application claims priority on the basis of Japanese application Japanese Patent Application No. 2019-126961, filed on July 8, 2019, and incorporates all of its disclosures herein.

<Additional notes>
Some or all of the above embodiments and examples may be described as, but not limited to, the following appendices.
(Appendix 1)
Campanula plants that are extracted in non-long-day cultivation.
(Appendix 2)
The campanula plant according to Appendix 1, wherein the non-long-day condition is a light condition in which the dark period is 13 hours or more.
(Appendix 3)
The campanula plant according to Appendix 1 or 2, wherein the non-long-day condition is a light condition in which the light period is 4 hours or more.
(Appendix 4)
The campanula plant according to any one of Appendix 1 to 3, wherein the drawing includes flowering.
(Appendix 5)
The campanula plant according to any one of Appendix 1 to 4, wherein the campanula plant is a campanula medium plant.
(Appendix 6)
The Campanula plant according to any one of Appendix 1 to 5, wherein the Campanula plant is a Campanula medium plant specified by Accession No. FERM BP-22375 or a progeny lineage.
(Appendix 7)
A Campanula medium plant identified by accession number FERM BP-22375 or a Campanula plant of its progeny lineage.
(Appendix 8)
The campanula plant according to any one of Appendix 1 to 7, wherein the campanula plant is a plant body or a part thereof.
(Appendix 9)
The campanula plant according to any one of Supplementary notes 1 to 8, wherein the campanula medium plant is a seed.
(Appendix 10)
Including a crossing step of crossing a first campanula plant with a second campanula plant.
The method for producing a campanula plant, wherein the first campanula plant is the campanula plant according to any one of Appendix 1 to 9.
(Appendix 11)
The production method according to Appendix 10, which comprises a selection step of cultivating the obtained progeny line under non-long-day conditions and selecting the extracted Campanula plants.
(Appendix 12)
The manufacturing method according to Appendix 11, wherein the non-long-day condition is a light condition in which the dark period is 13 hours or more.
(Appendix 13)
The production method according to Appendix 11 or 12, wherein the non-long day condition is a light condition in which the light period is 4 hours or more.
(Appendix 14)
The production method according to any one of Supplementary note 11 to 13, wherein the drawing includes flowering.
(Appendix 15)
A method for screening a non-long-day campanula plant, which comprises a selection step of cultivating the test campanula plant under non-long-day conditions and selecting the extracted campanula plant.
(Appendix 16)
The screening method according to Appendix 15, wherein the non-long-day condition is a light condition in which the dark period is 13 hours or more.
(Appendix 17)
The screening method according to Appendix 15 or 16, wherein the non-long day condition is a light condition in which the light period is 4 hours or more.
(Appendix 18)
The screening method according to any one of Appendix 15 to 17, wherein the drawing comprises flowering.
(Appendix 19)
The screening method according to any one of Appendix 15 to 18, wherein the test campanula plant is a campanula medium plant or a progeny line specified by accession number FERM BP-22375.
(Appendix 20)
A breeding step of crossing a first campanula plant with a second campanula plant to breed a progeny line that can be extracted in non-long-day cultivation is included.
The method for changing the drawing day length in a campanula plant, wherein the first campanula plant is the campanula plant according to any one of Appendix 1 to 9.

As described above, the campanula plant of the present invention can be extracted even under non-long-day conditions. Therefore, according to the campanula plant of the present invention, the campanula plant such as medium can be extracted even under short-day conditions such as winter without using an electric lighting facility. Therefore, according to the present invention, for example, labor and cost in cultivating a campanula plant can be reduced. Therefore, the present invention is extremely useful in the agricultural field such as breeding.

Claims (20)

Campanula plants that are extracted in non-long-day cultivation. The campanula plant according to claim 1, wherein the non-long-day condition is a light condition in which the dark period is 13 hours or more. The campanula plant according to claim 1 or 2, wherein the non-long day condition is a light condition in which the light period is 4 hours or more. The campanula plant according to any one of claims 1 to 3, wherein the drawing includes flowering. The campanula plant according to any one of claims 1 to 4, wherein the campanula plant is a campanula medium plant. The Campanula plant according to any one of claims 1 to 5, wherein the Campanula plant is a Campanula medium plant specified by Accession No. FERM BP-22375 or a progeny lineage. A Campanula medium plant identified by accession number FERM BP-22375 or a Campanula plant of its progeny lineage. The campanula plant according to any one of claims 1 to 7, wherein the campanula plant is a plant body or a part thereof. The campanula plant according to any one of claims 1 to 8, wherein the campanula medium plant is a seed. Including a crossing step of crossing a first campanula plant with a second campanula plant.
The method for producing a campanula plant, wherein the first campanula plant is the campanula plant according to any one of claims 1 to 9. The production method according to claim 10, further comprising a selection step of cultivating the obtained progeny line under non-long-day conditions and selecting the extracted Campanula plant. The production method according to claim 11, wherein the non-long day condition is a light condition in which the dark period is 13 hours or more. The production method according to claim 11 or 12, wherein the non-long day condition is a light condition in which the light period is 4 hours or more. The production method according to any one of claims 11 to 13, wherein the drawing includes flowering. A method for screening a non-long-day campanula plant, which comprises a selection step of cultivating the test campanula plant under non-long-day conditions and selecting the extracted campanula plant. The screening method according to claim 15, wherein the non-long day condition is a light condition in which the dark period is 13 hours or more. The screening method according to claim 15 or 16, wherein the non-long day condition is a light condition in which the light period is 4 hours or more. The screening method according to any one of claims 15 to 17, wherein the drawing includes flowering. The screening method according to any one of claims 15 to 18, wherein the test campanula plant is a campanula medium plant or a progeny line specified by accession number FERM BP-22375. A breeding step of crossing a first campanula plant with a second campanula plant to breed a progeny line that can be extracted in non-long-day cultivation is included.
The method for changing the drawing day length in a Campanula plant, wherein the first Campanula plant is the Campanula plant according to any one of claims 1 to 9.