KR100852241B1 - A new variety of plant, a dwarf cultivar of campanula spp., heundul - Google Patents

Abstract

A new variety of plant, a Dwarf Cultivar of Campanula spp., Heundul is provided to improve viability in nature, enhance usefulness for admiration by reducing the height of plant as compared with parent plants, and induce fast propagation period through in vitro mass proliferation. A new variety of plant, a Dwarf Cultivar of Campanula spp., Heundul is produced by breeding Campanula punctata Lam. as a mother plant with Campanula takesimana Nakai Seonginbong as a father plant, and has the plant properties of (1) plant height of 51.3cm, (2) leaf size of 3.5cm, (3) few radical leaves, (4) leaf shape of heart or triangle, (5) flower color of white, (6) flower with flower spray formed at 1-2 joints, and bell shaped corolla, (7) matured flowerpot with three-branched stigma, (8) flower bud differentiation condition of seed low temperature sensing, (9) seeding period of March, and (10) flowering time of March to May.

Description

A new variety of plant, a Dwarf Cultivar of Campanula spp., Heundul}

The present invention relates to a new type of plant dwarf lantern, rock bellflower, and more specifically, the present invention is the mother of Aeggi lantern flowers, the island length is more dwarf than the mother obtained by mating the island lantern flower "adult rod" father is good for breeding period This short new plant is about a dwarf bellflower, "wispy bellflower".

As urban people's interest in native plants grows, the use of these native plants in urban parks and apartment landscaping is increasing.

Campanula punctata was initially classified as a strain by Jussieu (1789) as a plant belonging to campanulaceae (Campanulaceae) campanulales (Campanulales), and is about 70 in 2,000 species distribution around the temperate regions of the northern hemisphere. Hemsley (1889) classified Korean perennial beech flowers into 5 genus 7 species, and Lee et al. (1988) reported that 9 genus 24 species grow in Korea. That is a kind of Campanula punctata (C. punctata Lam.), Island Campanula punctata (C. Takesimana), often Campanula punctata (C. punctata Lam. var . rubriflora Mak.), C. punctata Lam., unclassified, C. punctata Lam., unclassified, C. punctata var. albiflora , etc. (Park et al., 2002; Shim et al. , 2005). Especially, C. punctata Lam. With 2n = 34 chromosome, including 2n = 36 and Ulleungdo's island bellflower and purple bellflower flower are 5-7cm long, unlike other native plants. It is a long-lived, native plant that is highly valued for cutting, differentiating, and landscaping due to its polymorphism.

Like this, the bellflower is a native plant with high development value, but it has a disadvantage that it is inflated due to the weight of many fire extinguishers due to its long grass length and long-term flowering. In addition, it is difficult to commercialize by uniform seedlings because it takes a lot of time to temporarily seed and breed seeds as infinity plants. Therefore, in order to use the bellflower for differentiation, it is necessary to improve the coronary value by controlling the height, and to use and supply it as a real resource, it is necessary to develop an efficient breeding method and to obtain many individuals uniformly in a short time. In order to increase the ornamental value, it is desirable to set up the direction of improvement of native plants based on breeding between species, lines, or varieties prior to increasing the breeding materials such as various colors, burns, grass height, and cold resistance.

Therefore, the present inventors selected a superior mating parent and then tried to select a F ₁ bellflower plant in which the grass height was distorted through cross-breeding.

Therefore, an object of the present invention is to provide a new species of plant dwarf bellflower with herb length more dwarf than the mother through the hybrid species of Aegean bellflower and island bellflower "Adult Bong".

The above object of the present invention was achieved by breeding a hybrid hybrid F ₁ plant, a rocking bellflower, whose length is shorter than that of the mother seedlings, with the mother of Aeggi bellflower and the island bellflower "Adultbong" as father.

Hereinafter, the specific configuration of the invention will be described in detail.

The configuration of the present invention is the mother of agigi lantern flowers, the island lantern flower "Adult Bong" as a father and the stationary cross breeding to obtain a new species shorter than the mother plant and named it "shake bellflower"; Cultivating the new plant and examining the properties; And, after culturing the new varieties of plants in the cabin is configured to multiply through nutrition.

New varieties of plant dwarf bellflower, "Shake bellflower" of the present invention has the following characteristics were grown by mating the seedlings as a mother, a bellflower "Adult Bong" as a father, and then harvested the seeds, the form of a wrapping paper It is characterized by propagation as:

1.Extension: 51.3cm

2. Size of blade: 3.5cm

3. Root leaves: almost no root leaves

4. Lobe: heart or ovate triangle

5. Color: White

6. Flower: Flowering branch is formed from 1 ~ 2 node, flower shape is conical shape, corolla shape is short, voluminous bell shape, and it develops leaf shape near root.

7. Pollen: cut out into three branches as the mother matures

8. Flower differentiation condition: Seed cryogenicity

9. Planters: around March

10. Flowering period: 3 ~ 5 months

Hereinafter, the specific configuration of the present invention will be described through Examples, but the scope of the present invention is not limited to these Examples.

As the present invention will be described through the examples, the present invention relates to a new species of plant dwarf lanterns, rocking lantern flowers as a mother of agile bellflower, and is survivable in the natural state obtained by crossing the island bellflower "adult rod" as father It is a very useful invention for horticulture and flower industry because it has the effect of providing a new breed of plant dwarf bellflower, "rock bellflower", which has a shorter breeding period through in-flight mass growth.

Example  1: new varieties Dwarf lantern  sarcoma

Plants used to obtain dwarf varieties include Campanula punctata Lam., Collected at Jieun Wild Flower Farm (Goseong-gun, Gyeongnam), and island bell "Adultbong" ( C. takesimana ), collected in 1994 and 1995. Nakai Seonginbong). These materials were distributed and grown in glass greenhouse at Gyeongsang National University and used for the experiment. The cultivation medium was commercial soil [movie tossil medium, Shinangro Co., Ltd.], and planted in a plastic pot (Dongwoo Co., Ltd.) having a size of 10 cm x 15 cm, nutrient solution (Ca (NO ₃ ) ₂ · 4H ₂ O 472.0 mg / L, MgSO ₄ 7H ₂ O 246.0 mg / L, KNO ₃ 202.0 mg / L, NH ₄ NO ₃ 80.0mg / L, KH ₂ PO ₄ 272.0mg / L, Fe-EDTA 15.0mg / L, H ₃ BO ₃ 1.4 mg / L, CuSO ₄ · 5H ₂ O 0.2 mg / L, NaMgSO ₄ · 7H ₂ O 2.1 mg / L, NaMoO ₄ · 2H ₂ O 0.1 mg / L and ZnSO ₄ · 7H ₂ O 0.8 mg / L It was cultivated with two correlates with). In order to induce low temperature response for flower differentiation, the maximum temperature in winter was managed below 5 ℃.

In order to find out the flowering life of the island lantern flower "Adultbong" and Aegean lantern flower, we harvested 60-80cm tall flowers from the biennial plants of the flowering season at the weekly interval from March 20 to May 20, 2006. It was investigated at 9 am. Cut flower stems are re-cut in water and the preservation solution of distilled water, sodium hypochlorite [NaOCl, Lax, Yuhan-Lax Co., Ltd.], carbonated drink [Cider, Lotte Chilsung Co., Ltd.], or sodium hypochlorite + carbonated drink One by one into a 200mL measuring cylinder. After 8 hours of pretreatment, temperature 25 ± 1 ℃, relative humidity 30 ~ 60%, cool-white fluorescent lamp [Model FL 40EX-W, Seungsan Osram Co., Ltd.], brightness 45 μmolm ² s ^{- 1} and photoperiod 16 hours The conditions of growth regulation were investigated. Seven flower stems were inserted for each treatment, and the quality was examined by repeating seven times at weekly intervals.

In consideration of the flowering time of the selected bellflower plants, the bellflower (♀) x island bellflower "Adult Bong" (♂) was crossed. During the mating season, the island lantern "Adult Bong" began to bloom in late April 2001, and the Aegean lantern bloomed in early May.

After mating, the seeds of F ₁ were harvested from September 2001, and the seeds were stored in a refrigerator at 5 ° C and sown in March of the following year to use seedlings. The seedlings germinated afterwards are grown for 4 weeks under the conditions of 25 ± 1 ℃, 70 ~ 80% relative humidity, 45μmolm ² s ^-1 luminosity, and 16 hours of photoperiod (fluorescent lamp). Managed.

The F ₁ plants and plant height, leaf size, the length of the petiole, by selecting a noticeable plant phenotypic and morphological currency such hwagyeongjang Arabidopsis Campanula punctata (♀) × island F ₁ plants of Campanula punctata "Seonginbong" (♂) is "shake Campanula punctata (Heundul) ".

Example  2: Characteristics of New Varieties of Plant of the Present Invention

Botanical characteristics of the species "rocking bellflower" from Example 1 are shown in Table 1 compared with the mother example. In addition, the appearance, firearm shape and leaf shape of the adult plants of the rocking bellflower are shown in FIGS.

Comparison of Growth Characteristics of Parent and F ₁ Plants 15 Months After Sowing kind Extra long (cm) Color Flowerpot Leaf disease (cm) leaf Diameter (cm) Length (cm) Length (cm) Width (cm) Island lantern 84.2 ± 0.4 White 6.1 ± 0.3 6.6 ± 0.2 14.8 ± 0.7 6.6 ± 0.2 6.1 ± 0.3 Baby lantern flower 18.4 ± 0.6 White 2.6 ± 0.2 2.9 ± 0.2 10.0 ± 0.5 2.9 ± 0.2 2.6 ± 0.2 Rocking Lantern 51.3 ± 0.3 White 4.2 ± 0.2 3.5 ± 0.2 12.9 ± 0.5 3.5 ± 0.2 4.2 ± 0.2 * The figures in the table represent the mean ± standard deviation of 12 plants.

As shown in Table 1, the length of the peduncle was significantly reduced compared to the parent flower at the first flowering of the F ₁ plant. According to the degree of growth, the size of the leaves and the shape of the edges of the same plants were different compared to the parent, "Shake Lantern" was a relatively uniform size of the leaf between the parent and the copy. The length of the leaf disease was 12.9 cm in F ₁ plants. There were few root leaves running on the rosette stem. The lobes were mostly cardiac or egg-shaped triangles, and the emulsion was secreted at the time of cutting the lobe.

The color of the light shows a white phenotype, although the difference in brightness is visible. The F ₁ plant was in full bloom after 2 weeks of cultivation, and the flowers bloomed one by one at the end of the stem or axilla, eventually forming a cone-like cone. Pollen is also a mother-like form, and as the maturity splits into three branches. Flowers are branched from 1 to 2 nodes, the stem is conical, and the corolla is short and has a voluminous bell shape. It is developed in the form of foliage from the roots.

To compare the color change of the leaves and the leaves after the growth of the F- ₁ plant, which was collected from the flowers, the Hunter L (Lightness: Black = 0, White = 100), a using a color difference meter (CM-3500d, Minolta, Japan) (Red-Green: Red = + 100, Green = 80) and b (Yellow-Blue: Yellow = + 70, Blue = -70) values were measured. The measured results were statistically tested by Duncan's multiple test using Excel (Excel 2000, Microsoft, USA) and SAS (Statistical Analysis System, V. 6.01, Cary, NC, USA).

As shown in Table 2, L, a, and b values were higher as the color was closer to white, such as island bellflower.

Color Comparison of Parent and F ₁ Plants 15 Months After Sowing kind Hunter's value L a b Island lantern 78.2 ^a 9.7 ^d 23.3 ^a Baby lantern flower 70.1 ^b 6.0 ^e 3.1 ^de Rocking Lantern 77.4 ^a 3.2 ^fg 5.4 ^c

z: Letters on each line represent significant differences by Duncan's majority range test (P = 0.05)

Example  3: Asexual breeding of new varieties of the present invention

Seedlings used as explants are cultivated to prevent pests and to grow robustly.

Remove the flower tip of the apical stem from the flower stem (flower stalks) and incubate by using the axillary bud below it as an explant, or by seeding the seed stored at 5 ℃.

When sowing seeds, the seeds stored at 5 ° C were surface sterilized in 70% ethanol and 1% sodium hypochloride solution for 1 minute each, rinsed several times with sterile water, and then seeded in MS basic medium, and then grown. Aseptic germination in control phase (temperature 25 ± 1 ℃, relative humidity 70 ~ 80%, intensity 45 μmolm ² s ^-1 , and photoperiod 16 hours (fluorescent lamp), Goryeo, Seoul) The seedlings were selected and healed 4 per culture vessel. After that, it was managed by multiplying through the breeding in the form of a cloth.

1 is a photograph showing a new plant "rocking bellflower" of the present invention compared to the parent plant by growing in a pot.

Figure 2 is a photograph showing the firearm of the new species of plant "rocking bellflower" of the present invention.

Figure 3 is a photograph showing the leaves of the new breed plant "rocking bellflower" of the present invention.

Claims (1)

A new species of plant dwarf bellflower, "Shake bellflower," which has the following characteristics: ": 1.Extension: 51.3cm 2. Size of blade: 3.5cm 3. Root leaves: almost no root leaves 4. Lobe: heart or ovate triangle 5. Color: White 6. Flower: Flowering branch is formed from 1 ~ 2 node, flower shape is conical shape, corolla shape is short, voluminous bell shape, and it develops leaf shape near root. 7. Pollen: cut out into three branches as the mother matures 8. Flower differentiation condition: Seed cryogenicity 9. Planters: around March 10. Flowering period: 3 ~ 5 months

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