JP2020536520A - Wood−ljungdahl微生物におけるポリヒドロキシブチレートの生成 - Google Patents
Wood−ljungdahl微生物におけるポリヒドロキシブチレートの生成 Download PDFInfo
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Abstract
Description
本出願は、2017年10月4日に出願された米国特許出願62/568,127の利益を主張し、その全体は参照により本明細書に組み込まれる。
経路
微生物
酵素
基質
生成物
発酵
実施例1
実施例2
実施例3
実施例4
実施例5
実施例6
Claims (20)
- 非自然発生型のWood−Ljungdahl微生物であって、
a.アセチル−CoAをアセトアセチル−CoAに変換する酵素と、
b.アセトアセチル−CoAを3−ヒドロキシブチリル−CoAに変換する酵素と、
c.3−ヒドロキシブチリル−CoAをポリヒドロキシブチレートに変換する酵素と、を含む、微生物。 - アセチル−CoAをアセトアセチル−CoAに変換する前記酵素が、アセチル−CoA C−アセチルトランスフェラーゼ(EC2.3.1.9)である、請求項1に記載の微生物。
- 前記アセチル−CoA C−アセチルトランスフェラーゼが、Acinetobacter baumannii、Aeromonas hydrophilia、Alcaligenes latus、Arthrospira platensis、Bacillus subtilis、Burkholderia cepacia、Clostridium acetobutylicum、Cupriavidus necator、Escherichia coli、Haloferax mediterranei、Pseudomonas aeruginosa、Pseudomonas fluorescens、Pseudomonas mandelii、Pseudomonas oleovorans、Pseudomonas putida、またはStreptomyces coelicolorに由来する、請求項2に記載の微生物。
- アセトアセチル−CoAを3−ヒドロキシブチリル−CoAに変換する前記酵素が、アセトアセチル−CoAレダクターゼ(EC1.1.1.36)または3−ヒドロキシブチリル−CoAデヒドロゲナーゼ(EC1.1.1.157)である、請求項1に記載の微生物。
- 前記アセトアセチル−CoAレダクターゼが、Acinetobacter baumannii、Aeromonas hydrophilia、Alcaligenes latus、Arthrospira platensis、Bacillus subtilis、Burkholderia cepacia、Cupriavidus necator、Haloferax mediterranei、Pseudomonas aeruginosa、Pseudomonas fluorescens、Pseudomonas mandelii、Pseudomonas oleovorans、Pseudomonas putida、またはStreptomyces coelicolorに由来する、請求項4に記載の微生物。
- 前記3−ヒドロキシブチリル−CoAデヒドロゲナーゼが、Clostridium beijerinckii、Clostridium acetobutylicum、またはClostridium kluyveriに由来する、請求項4に記載の微生物。
- 3−ヒドロキシブチリル−CoAをポリヒドロキシブチレートに変換する前記酵素が、ポリポリヒドロキシアルカノエートシンターゼ(EC2.3.1.−)である、請求項1に記載の微生物。
- 前記ポリヒドロキシアルカノエートシンターゼが、Acinetobacter baumannii、Aeromonas caviae、Aeromonas hydrophilia、Alcaligenes latus、Arthrospira platensis、Bacillus subtilis、Burkholderia cepacia、Cupriavidus necator、Haloferax mediterranei、Pseudomonas aeruginosa、Pseudomonas fluorescens、Pseudomonas mandelii、Pseudomonas oleovorans、Pseudomonas putida、Pseudomonas属61−3、Rhodospirillum rubrum、またはStreptomyces coelicoloに由来する、請求項7に記載の微生物。
- 前記微生物が、Acetobacterium、Alkalibaculum、Blautia、Butyribacterium、Clostridium、Eubacterium、Moorella、Oxobacter、Sporomusa、およびThermoanaerobacterからなる群から選択される属のメンバーである、請求項1に記載の微生物。
- 前記微生物が、Acetobacterium woodii、Alkalibaculum bacchii、Blautia producta、Butyribacterium methylotrophicum、Clostridium aceticum、Clostridium autoethanogenum、Clostridium carboxidivorans、Clostridium coskatii、Clostridium drakei、Clostridium formicoaceticum、Clostridium ljungdahlii、Clostridium magnum、Clostridium ragsdalei、Clostridium scatologenes、Eubacterium limosum、Moorella thermautotrophica、Moorella thermoacetica、Oxobacter pfennigii、Sporomusa ovata、Sporomusa silvacetica、Sporomusa sphaeroides、およびThermoanaerobacter kiuviからなる群から選択される親微生物に由来する、請求項1に記載の微生物。
- 前記微生物が、Clostridium autoethanogenum、Clostridium coskatii、Clostridium ljungdahlii、およびClostridium ragsdaleiからなる群から選択される親細菌に由来する、請求項10に記載の微生物。
- 前記微生物が、CO、CO2、およびH2のうちの1つ以上を含むガス状基質を消費する、請求項1に記載の微生物。
- 前記微生物が、嫌気性である、請求項1に記載の微生物。
- 前記微生物が、ポリヒドロキシブチレートを分解することができない、請求項1に記載の微生物。
- 前記微生物が、光栄養性、光合成性、またはメタン資化性ではない、請求項1に記載の微生物。
- ガス状基質の存在下で請求項1に記載の微生物を培養し、それにより前記微生物がポリヒドロキシブチレートを生成することを含む、ポリヒドロキシブチレートを生成する方法。
- 前記ガス状基質が、CO、CO2、およびH2のうちの1つ以上を含む、請求項16に記載の方法。
- 前記培養することが、嫌気性条件下で実行される、請求項16に記載の方法。
- 前記培養することが、炭水化物基質の不在下で実行される、請求項16に記載の方法。
- 前記培養することが、光の不在下で実行される、請求項16に記載の方法。
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