JP2020516232A5 - - Google Patents
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- JP2020516232A5 JP2020516232A5 JP2019531917A JP2019531917A JP2020516232A5 JP 2020516232 A5 JP2020516232 A5 JP 2020516232A5 JP 2019531917 A JP2019531917 A JP 2019531917A JP 2019531917 A JP2019531917 A JP 2019531917A JP 2020516232 A5 JP2020516232 A5 JP 2020516232A5
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- JP
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- Prior art keywords
- item
- detection
- nucleic acid
- overhang
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- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000001514 detection method Methods 0.000 claims description 136
- 108020004707 nucleic acids Proteins 0.000 claims description 124
- 102000039446 nucleic acids Human genes 0.000 claims description 124
- 150000007523 nucleic acids Chemical class 0.000 claims description 124
- 238000000034 method Methods 0.000 claims description 95
- 239000000203 mixture Substances 0.000 claims description 89
- 230000000295 complement effect Effects 0.000 claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 21
- 102000004169 proteins and genes Human genes 0.000 claims description 21
- 238000009396 hybridization Methods 0.000 claims description 15
- 239000002773 nucleotide Substances 0.000 claims description 15
- 125000003729 nucleotide group Chemical group 0.000 claims description 15
- 230000000692 anti-sense effect Effects 0.000 claims description 13
- 239000003446 ligand Substances 0.000 claims description 11
- 102000018697 Membrane Proteins Human genes 0.000 claims 1
- 108010052285 Membrane Proteins Proteins 0.000 claims 1
- 230000001351 cycling effect Effects 0.000 claims 1
- 238000003556 assay Methods 0.000 description 15
- 210000001519 tissue Anatomy 0.000 description 14
- 239000007894 caplet Substances 0.000 description 13
- 238000010362 genome editing Methods 0.000 description 9
- 108020004414 DNA Proteins 0.000 description 8
- 102000004506 Blood Proteins Human genes 0.000 description 6
- 108010017384 Blood Proteins Proteins 0.000 description 6
- 108091033409 CRISPR Proteins 0.000 description 6
- 102000014914 Carrier Proteins Human genes 0.000 description 6
- 108010078791 Carrier Proteins Proteins 0.000 description 6
- 101710163270 Nuclease Proteins 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 102000012410 DNA Ligases Human genes 0.000 description 5
- 108010061982 DNA Ligases Proteins 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- QGKMIGUHVLGJBR-UHFFFAOYSA-M (4z)-1-(3-methylbutyl)-4-[[1-(3-methylbutyl)quinolin-1-ium-4-yl]methylidene]quinoline;iodide Chemical compound [I-].C12=CC=CC=C2N(CCC(C)C)C=CC1=CC1=CC=[N+](CCC(C)C)C2=CC=CC=C12 QGKMIGUHVLGJBR-UHFFFAOYSA-M 0.000 description 4
- GOLORTLGFDVFDW-UHFFFAOYSA-N 3-(1h-benzimidazol-2-yl)-7-(diethylamino)chromen-2-one Chemical compound C1=CC=C2NC(C3=CC4=CC=C(C=C4OC3=O)N(CC)CC)=NC2=C1 GOLORTLGFDVFDW-UHFFFAOYSA-N 0.000 description 4
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 description 4
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 4
- QDLAGTHXVHQKRE-UHFFFAOYSA-N lichenxanthone Natural products COC1=CC(O)=C2C(=O)C3=C(C)C=C(OC)C=C3OC2=C1 QDLAGTHXVHQKRE-UHFFFAOYSA-N 0.000 description 4
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- HLXHCNWEVQNNKA-UHFFFAOYSA-N 5-methoxy-2,3-dihydro-1h-inden-2-amine Chemical compound COC1=CC=C2CC(N)CC2=C1 HLXHCNWEVQNNKA-UHFFFAOYSA-N 0.000 description 3
- 101710159080 Aconitate hydratase A Proteins 0.000 description 3
- 101710159078 Aconitate hydratase B Proteins 0.000 description 3
- 102000011767 Acute-Phase Proteins Human genes 0.000 description 3
- 108010062271 Acute-Phase Proteins Proteins 0.000 description 3
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 3
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 3
- 238000010354 CRISPR gene editing Methods 0.000 description 3
- 101000709520 Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B) Atypical response regulator protein ChxR Proteins 0.000 description 3
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 3
- 101710096438 DNA-binding protein Proteins 0.000 description 3
- 102100021238 Dynamin-2 Human genes 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 3
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 3
- 101000817607 Homo sapiens Dynamin-2 Proteins 0.000 description 3
- 102000004310 Ion Channels Human genes 0.000 description 3
- 102000044126 RNA-Binding Proteins Human genes 0.000 description 3
- 101710105008 RNA-binding protein Proteins 0.000 description 3
- 102000039471 Small Nuclear RNA Human genes 0.000 description 3
- 108020004688 Small Nuclear RNA Proteins 0.000 description 3
- 150000001412 amines Chemical class 0.000 description 3
- 230000002337 anti-port Effects 0.000 description 3
- 239000003114 blood coagulation factor Substances 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 230000021164 cell adhesion Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 108091027963 non-coding RNA Proteins 0.000 description 3
- 102000042567 non-coding RNA Human genes 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000002096 quantum dot Substances 0.000 description 3
- -1 rRNA Proteins 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000008685 targeting Effects 0.000 description 3
- 230000032895 transmembrane transport Effects 0.000 description 3
- 239000004971 Cross linker Substances 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 208000014644 Brain disease Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000006994 Precancerous Conditions Diseases 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
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- 230000002496 gastric effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000017169 kidney disease Diseases 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022020366A JP2022058977A (ja) | 2016-12-16 | 2022-02-14 | ライゲーション増幅を使用する分子検出 |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662435424P | 2016-12-16 | 2016-12-16 | |
| US62/435,424 | 2016-12-16 | ||
| US201762480107P | 2017-03-31 | 2017-03-31 | |
| US62/480,107 | 2017-03-31 | ||
| US201762509995P | 2017-05-23 | 2017-05-23 | |
| US62/509,995 | 2017-05-23 | ||
| PCT/US2017/066841 WO2018112422A1 (en) | 2016-12-16 | 2017-12-15 | Molecular detection using ligation amplification |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022020366A Division JP2022058977A (ja) | 2016-12-16 | 2022-02-14 | ライゲーション増幅を使用する分子検出 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020516232A JP2020516232A (ja) | 2020-06-11 |
| JP2020516232A5 true JP2020516232A5 (enExample) | 2021-01-28 |
| JP7093355B2 JP7093355B2 (ja) | 2022-06-29 |
Family
ID=62559496
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019531917A Active JP7093355B2 (ja) | 2016-12-16 | 2017-12-15 | ライゲーション増幅を使用する分子検出 |
| JP2022020366A Pending JP2022058977A (ja) | 2016-12-16 | 2022-02-14 | ライゲーション増幅を使用する分子検出 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022020366A Pending JP2022058977A (ja) | 2016-12-16 | 2022-02-14 | ライゲーション増幅を使用する分子検出 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10781477B2 (enExample) |
| EP (3) | EP4328323A3 (enExample) |
| JP (2) | JP7093355B2 (enExample) |
| CN (2) | CN110300806B (enExample) |
| CA (1) | CA3047127A1 (enExample) |
| DK (2) | DK3929304T3 (enExample) |
| FI (1) | FI3929304T3 (enExample) |
| WO (1) | WO2018112422A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7093355B2 (ja) * | 2016-12-16 | 2022-06-29 | アラトム， エルエルシー | ライゲーション増幅を使用する分子検出 |
| US12571028B2 (en) * | 2019-02-15 | 2026-03-10 | Advanced Cell Diagnostics, Inc. | Methods for multiplex detection of nucleic acids by in situ hybridization |
| US11076219B2 (en) * | 2019-04-12 | 2021-07-27 | Bose Corporation | Automated control of noise reduction or noise masking |
| US20240167081A1 (en) * | 2022-11-08 | 2024-05-23 | 10X Genomics,Inc. | Immobilization methods and compositions for in situ detection |
| EP4653542A1 (en) * | 2024-05-21 | 2025-11-26 | Leica Microsystems CMS GmbH | Label, marker and method for analysing a biological sample |
Family Cites Families (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US317077A (en) * | 1885-05-05 | belcher | ||
| CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
| US6011020A (en) * | 1990-06-11 | 2000-01-04 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand complexes |
| US5386020A (en) * | 1991-01-10 | 1995-01-31 | New York University | Multiply connected, three-dimensional nucleic acid structures |
| US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6072044A (en) * | 1996-04-26 | 2000-06-06 | New York University | Nanoconstructions of geometrical objects and lattices from antiparallel nucleic acid double crossover molecules |
| CA2259918A1 (en) * | 1996-07-12 | 1998-01-22 | Tm Technologies, Inc. | Signal amplification method |
| US6261771B1 (en) * | 1998-02-18 | 2001-07-17 | The United States Of America As Represented By The Secretary Of The Army | Method and apparatus for detection of multiple nucleic acid sequences and multiple antigens |
| US6403309B1 (en) * | 1999-03-19 | 2002-06-11 | Valigen (Us), Inc. | Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays |
| JP2001013139A (ja) | 1999-06-28 | 2001-01-19 | Toyobo Co Ltd | 免疫学的測定法 |
| US6686461B1 (en) * | 2000-03-22 | 2004-02-03 | Solulink Bioscience, Inc. | Triphosphate oligonucleotide modification reagents and uses thereof |
| EP1287165B1 (en) * | 2000-06-02 | 2007-06-13 | Bayer Corporation | Method for detection and localization of genes in situ using branched-DNA hybridisation |
| US6531283B1 (en) | 2000-06-20 | 2003-03-11 | Molecular Staging, Inc. | Protein expression profiling |
| DE10201886B4 (de) * | 2001-01-19 | 2014-01-23 | Honda Giken Kogyo K.K. | Polymerelektrolytmembran sowie Verfahren zur Herstellung dieser und Festpolymerelektrolytbrennstoffzelle, welche diese verwendet |
| US20020132260A1 (en) | 2001-02-22 | 2002-09-19 | Erlander Mark G. | Quantitative immunohistochemistry (QIHC) |
| US20030082584A1 (en) * | 2001-06-29 | 2003-05-01 | Liang Shi | Enzymatic ligation-based identification of transcript expression |
| WO2003031591A2 (en) | 2001-10-10 | 2003-04-17 | Superarray Bioscience Corporation | Detecting targets by unique identifier nucleotide tags |
| CA2363703A1 (en) * | 2001-11-23 | 2003-05-23 | Royce Technologies Llc, A Nevada Corporation | Method for amplification of molecular bio-assay signals |
| WO2005010494A2 (en) | 2003-07-21 | 2005-02-03 | Jie Wu | Multiplexed analyte detection |
| US7727721B2 (en) | 2005-03-08 | 2010-06-01 | California Institute Of Technology | Hybridization chain reaction amplification for in situ imaging |
| US8241874B2 (en) * | 2005-05-04 | 2012-08-14 | Wayne State University | Rolling circle amplification |
| US20070048759A1 (en) * | 2005-06-10 | 2007-03-01 | Dan Luo | Detection of target molecules with labeled nucleic acid detection molecules |
| US20080038725A1 (en) | 2005-06-20 | 2008-02-14 | Yuling Luo | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations |
| DE602006020577D1 (de) | 2005-07-01 | 2011-04-21 | Dako Denmark As | Monomere und polymere linker zur konjugierung von biologischen molekülen und anderen stoffen |
| JP5537034B2 (ja) * | 2005-12-23 | 2014-07-02 | ナノストリング テクノロジーズ，インコーポレーテッド | ナノレポーターならびにその作製方法および使用方法 |
| US9315536B2 (en) * | 2006-08-29 | 2016-04-19 | Creatrone, Inc. | Self-assembling oligonucleotides and methods |
| ES2905054T3 (es) * | 2006-11-01 | 2022-04-06 | Ventana Med Syst Inc | Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso |
| US8309306B2 (en) * | 2008-11-12 | 2012-11-13 | Nodality, Inc. | Detection composition |
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| EP2539355B1 (en) * | 2010-02-26 | 2016-10-05 | Ventana Medical Systems, Inc. | In-situ hybridization with polytag probes |
| US9512471B2 (en) * | 2010-06-30 | 2016-12-06 | Diacarta Inc | Methods and kits for detecting human papillomavirus |
| EP3034625B1 (en) | 2010-10-21 | 2017-10-04 | Advanced Cell Diagnostics, Inc. | An ultra sensitive method for in situ detection of nucleic acids |
| US9556473B2 (en) * | 2011-02-15 | 2017-01-31 | Leica Biosystems Newcastle Ltd | Methods for identifying nucleic acid sequences |
| US8946389B2 (en) | 2011-04-25 | 2015-02-03 | University Of Washington | Compositions and methods for multiplex biomarker profiling |
| GB201108678D0 (en) * | 2011-05-24 | 2011-07-06 | Olink Ab | Multiplexed proximity ligation assay |
| US9651549B2 (en) * | 2012-07-13 | 2017-05-16 | Genisphere, Llc | Lateral flow assays using DNA dendrimers |
| GB201218909D0 (en) | 2012-10-22 | 2012-12-05 | Univ Singapore | Assay for the parallel detection of biological material based on PCR |
| WO2014145620A2 (en) * | 2013-03-15 | 2014-09-18 | The Broad Institute, Inc. | Novel hybridization probes and uses thereof |
| KR20160039229A (ko) * | 2013-07-30 | 2016-04-08 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 정량적 dna-기반 영상화 및 초해상도 영상화 |
| DE102014001685A1 (de) * | 2014-02-07 | 2015-08-13 | Kiekert Aktiengesellschaft | Kraftfahrzeugschloss mit integrierter Zuziehhilfe |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| WO2016057842A2 (en) | 2014-10-08 | 2016-04-14 | Aratome, LLC | High resolution imaging of tissue proteins |
| EP3362462B1 (en) | 2015-10-12 | 2021-07-21 | Advanced Cell Diagnostics, Inc. | In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto |
| CN105353131B (zh) | 2015-10-23 | 2017-04-19 | 山东大学 | 基于双编码和单分子计数的细胞因子多重检测方法 |
| CN119574873A (zh) * | 2016-05-02 | 2025-03-07 | Encodia公司 | 采用核酸编码的大分子分析 |
| EP3481843B1 (en) * | 2016-07-05 | 2022-01-26 | California Institute of Technology | Fractional initiator hybridization chain reaction |
| KR102363716B1 (ko) * | 2016-09-26 | 2022-02-18 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| JP7093355B2 (ja) * | 2016-12-16 | 2022-06-29 | アラトム， エルエルシー | ライゲーション増幅を使用する分子検出 |
-
2017
- 2017-12-15 JP JP2019531917A patent/JP7093355B2/ja active Active
- 2017-12-15 EP EP23190368.3A patent/EP4328323A3/en active Pending
- 2017-12-15 WO PCT/US2017/066841 patent/WO2018112422A1/en not_active Ceased
- 2017-12-15 CN CN201780086781.2A patent/CN110300806B/zh active Active
- 2017-12-15 EP EP17881278.0A patent/EP3555313B1/en active Active
- 2017-12-15 CA CA3047127A patent/CA3047127A1/en active Pending
- 2017-12-15 FI FIEP21177377.5T patent/FI3929304T3/fi active
- 2017-12-15 DK DK21177377.5T patent/DK3929304T3/da active
- 2017-12-15 EP EP21177377.5A patent/EP3929304B1/en active Active
- 2017-12-15 DK DK17881278.0T patent/DK3555313T3/da active
- 2017-12-15 CN CN202410215614.5A patent/CN118086461A/zh active Pending
-
2019
- 2019-01-23 US US16/254,926 patent/US10781477B2/en active Active
-
2020
- 2020-07-27 US US16/939,921 patent/US20200377933A1/en active Pending
-
2022
- 2022-02-14 JP JP2022020366A patent/JP2022058977A/ja active Pending
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