FI3929304T3 - Molekulaarin tunnistaminen käyttämällä ligatiovahvistusta - Google Patents
Molekulaarin tunnistaminen käyttämällä ligatiovahvistusta Download PDFInfo
- Publication number
- FI3929304T3 FI3929304T3 FIEP21177377.5T FI21177377T FI3929304T3 FI 3929304 T3 FI3929304 T3 FI 3929304T3 FI 21177377 T FI21177377 T FI 21177377T FI 3929304 T3 FI3929304 T3 FI 3929304T3
- Authority
- FI
- Finland
- Prior art keywords
- composition according
- nucleic acid
- stranded nucleic
- detector
- ligand
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title claims 2
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 239000003446 ligand Substances 0.000 claims 6
- 230000000692 anti-sense effect Effects 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- 238000006352 cycloaddition reaction Methods 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 2
- RPTUSVTUFVMDQK-UHFFFAOYSA-N Hidralazin Chemical compound C1=CC=C2C(NN)=NN=CC2=C1 RPTUSVTUFVMDQK-UHFFFAOYSA-N 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- RMTXUPIIESNLPW-UHFFFAOYSA-N 1,2-dihydroxy-3-(pentadeca-8,11-dienyl)benzene Natural products CCCC=CCC=CCCCCCCCC1=CC=CC(O)=C1O RMTXUPIIESNLPW-UHFFFAOYSA-N 0.000 claims 1
- UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 claims 1
- QARRXYBJLBIVAK-UEMSJJPVSA-N 3-[(8e,11e)-pentadeca-8,11-dienyl]benzene-1,2-diol;3-[(8e,11e)-pentadeca-8,11,14-trienyl]benzene-1,2-diol;3-[(8e,11e,13e)-pentadeca-8,11,13-trienyl]benzene-1,2-diol;3-[(e)-pentadec-8-enyl]benzene-1,2-diol;3-pentadecylbenzene-1,2-diol Chemical compound CCCCCCCCCCCCCCCC1=CC=CC(O)=C1O.CCCCCC\C=C\CCCCCCCC1=CC=CC(O)=C1O.CCC\C=C\C\C=C\CCCCCCCC1=CC=CC(O)=C1O.C\C=C\C=C\C\C=C\CCCCCCCC1=CC=CC(O)=C1O.OC1=CC=CC(CCCCCCC\C=C\C\C=C\CC=C)=C1O QARRXYBJLBIVAK-UEMSJJPVSA-N 0.000 claims 1
- IYROWZYPEIMDDN-UHFFFAOYSA-N 3-n-pentadec-8,11,13-trienyl catechol Natural products CC=CC=CCC=CCCCCCCCC1=CC=CC(O)=C1O IYROWZYPEIMDDN-UHFFFAOYSA-N 0.000 claims 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims 1
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 1
- 150000001345 alkine derivatives Chemical class 0.000 claims 1
- 150000001448 anilines Chemical class 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 150000001540 azides Chemical class 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 229960002474 hydralazine Drugs 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 150000003852 triazoles Chemical class 0.000 claims 1
- DQTMTQZSOJMZSF-UHFFFAOYSA-N urushiol Natural products CCCCCCCCCCCCCCCC1=CC=CC(O)=C1O DQTMTQZSOJMZSF-UHFFFAOYSA-N 0.000 claims 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/20—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans from protozoa
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
- C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
- C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662435424P | 2016-12-16 | 2016-12-16 | |
| US201762480107P | 2017-03-31 | 2017-03-31 | |
| US201762509995P | 2017-05-23 | 2017-05-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| FI3929304T3 true FI3929304T3 (fi) | 2024-03-27 |
Family
ID=62559496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FIEP21177377.5T FI3929304T3 (fi) | 2016-12-16 | 2017-12-15 | Molekulaarin tunnistaminen käyttämällä ligatiovahvistusta |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10781477B2 (enExample) |
| EP (3) | EP3555313B1 (enExample) |
| JP (2) | JP7093355B2 (enExample) |
| CN (2) | CN110300806B (enExample) |
| CA (1) | CA3047127A1 (enExample) |
| DK (2) | DK3929304T3 (enExample) |
| FI (1) | FI3929304T3 (enExample) |
| WO (1) | WO2018112422A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3929304T3 (da) * | 2016-12-16 | 2024-04-08 | Aratome Llc | Molekylær detektering ved ligeringsforstærkning |
| US20220154262A1 (en) * | 2019-02-15 | 2022-05-19 | Advanced Cell Diagnostics, Inc. | Methods for multiplex detection of nucleic acids by in situ hybridization |
| US11076219B2 (en) * | 2019-04-12 | 2021-07-27 | Bose Corporation | Automated control of noise reduction or noise masking |
| US20240167081A1 (en) * | 2022-11-08 | 2024-05-23 | 10X Genomics,Inc. | Immobilization methods and compositions for in situ detection |
| EP4653542A1 (en) * | 2024-05-21 | 2025-11-26 | Leica Microsystems CMS GmbH | Label, marker and method for analysing a biological sample |
Family Cites Families (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US317077A (en) * | 1885-05-05 | belcher | ||
| CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
| US6011020A (en) * | 1990-06-11 | 2000-01-04 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand complexes |
| US5386020A (en) * | 1991-01-10 | 1995-01-31 | New York University | Multiply connected, three-dimensional nucleic acid structures |
| US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6072044A (en) * | 1996-04-26 | 2000-06-06 | New York University | Nanoconstructions of geometrical objects and lattices from antiparallel nucleic acid double crossover molecules |
| AU4040097A (en) * | 1996-07-12 | 1998-02-09 | Tm Technologies, Inc. | Signal amplification method |
| US6261771B1 (en) * | 1998-02-18 | 2001-07-17 | The United States Of America As Represented By The Secretary Of The Army | Method and apparatus for detection of multiple nucleic acid sequences and multiple antigens |
| US6403309B1 (en) * | 1999-03-19 | 2002-06-11 | Valigen (Us), Inc. | Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays |
| JP2001013139A (ja) | 1999-06-28 | 2001-01-19 | Toyobo Co Ltd | 免疫学的測定法 |
| US6686461B1 (en) * | 2000-03-22 | 2004-02-03 | Solulink Bioscience, Inc. | Triphosphate oligonucleotide modification reagents and uses thereof |
| ES2287134T3 (es) * | 2000-06-02 | 2007-12-16 | Bayer Corporation | Procedimiento para la deteccion y localizacion de genes in situ usando hibridacion de adn ramificado. |
| US6531283B1 (en) | 2000-06-20 | 2003-03-11 | Molecular Staging, Inc. | Protein expression profiling |
| DE10201886B4 (de) * | 2001-01-19 | 2014-01-23 | Honda Giken Kogyo K.K. | Polymerelektrolytmembran sowie Verfahren zur Herstellung dieser und Festpolymerelektrolytbrennstoffzelle, welche diese verwendet |
| US20020132260A1 (en) | 2001-02-22 | 2002-09-19 | Erlander Mark G. | Quantitative immunohistochemistry (QIHC) |
| US20030082584A1 (en) * | 2001-06-29 | 2003-05-01 | Liang Shi | Enzymatic ligation-based identification of transcript expression |
| AU2002360272A1 (en) | 2001-10-10 | 2003-04-22 | Superarray Bioscience Corporation | Detecting targets by unique identifier nucleotide tags |
| CA2363703A1 (en) * | 2001-11-23 | 2003-05-23 | Royce Technologies Llc, A Nevada Corporation | Method for amplification of molecular bio-assay signals |
| EP1660858A4 (en) | 2003-07-21 | 2007-10-24 | Amplified Proteomics Inc | MULTIPLEX analyte |
| US7727721B2 (en) | 2005-03-08 | 2010-06-01 | California Institute Of Technology | Hybridization chain reaction amplification for in situ imaging |
| US8241874B2 (en) * | 2005-05-04 | 2012-08-14 | Wayne State University | Rolling circle amplification |
| US20070048759A1 (en) * | 2005-06-10 | 2007-03-01 | Dan Luo | Detection of target molecules with labeled nucleic acid detection molecules |
| WO2007001986A2 (en) | 2005-06-20 | 2007-01-04 | Yuling Luo | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations |
| WO2007045998A2 (en) | 2005-07-01 | 2007-04-26 | Dako Denmark A/S | New nucleic acid base pairs |
| EP1963531B1 (en) | 2005-12-23 | 2011-09-21 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| US9315536B2 (en) * | 2006-08-29 | 2016-04-19 | Creatrone, Inc. | Self-assembling oligonucleotides and methods |
| DK2078197T3 (en) * | 2006-11-01 | 2016-05-23 | Ventana Med Syst Inc | Haptenes, hapten conjugates, compositions thereof and processes for their preparation and use |
| US8309306B2 (en) * | 2008-11-12 | 2012-11-13 | Nodality, Inc. | Detection composition |
| ATE523603T1 (de) * | 2008-11-21 | 2011-09-15 | Chimera Biotec Gmbh | Konjugatkomplexe zum analytnachweis |
| WO2011106583A1 (en) * | 2010-02-26 | 2011-09-01 | Ventana Medical Systems, Inc. | Polytag probes |
| US9512471B2 (en) * | 2010-06-30 | 2016-12-06 | Diacarta Inc | Methods and kits for detecting human papillomavirus |
| ES2648564T3 (es) | 2010-10-21 | 2018-01-04 | Advanced Cell Diagnostics, Inc. | Método ultrasensible para la detección in situ de ácidos nucleicos |
| US9556473B2 (en) * | 2011-02-15 | 2017-01-31 | Leica Biosystems Newcastle Ltd | Methods for identifying nucleic acid sequences |
| US8946389B2 (en) | 2011-04-25 | 2015-02-03 | University Of Washington | Compositions and methods for multiplex biomarker profiling |
| GB201108678D0 (en) * | 2011-05-24 | 2011-07-06 | Olink Ab | Multiplexed proximity ligation assay |
| US9651549B2 (en) * | 2012-07-13 | 2017-05-16 | Genisphere, Llc | Lateral flow assays using DNA dendrimers |
| GB201218909D0 (en) | 2012-10-22 | 2012-12-05 | Univ Singapore | Assay for the parallel detection of biological material based on PCR |
| WO2014145620A2 (en) * | 2013-03-15 | 2014-09-18 | The Broad Institute, Inc. | Novel hybridization probes and uses thereof |
| HK1221747A1 (zh) * | 2013-07-30 | 2017-06-09 | 哈佛学院院长及董事 | 基於dna的定量成像和超分辨成像 |
| DE102014001685A1 (de) * | 2014-02-07 | 2015-08-13 | Kiekert Aktiengesellschaft | Kraftfahrzeugschloss mit integrierter Zuziehhilfe |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| WO2016057842A2 (en) * | 2014-10-08 | 2016-04-14 | Aratome, LLC | High resolution imaging of tissue proteins |
| US11078528B2 (en) | 2015-10-12 | 2021-08-03 | Advanced Cell Diagnostics, Inc. | In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto |
| CN105353131B (zh) | 2015-10-23 | 2017-04-19 | 山东大学 | 基于双编码和单分子计数的细胞因子多重检测方法 |
| KR102741603B1 (ko) * | 2016-05-02 | 2024-12-16 | 엔코디아, 인코포레이티드 | 암호화 핵산을 사용한 거대분자 분석 |
| IL290679B2 (en) * | 2016-07-05 | 2023-10-01 | California Inst Of Techn | Hybridization chain reaction based on a minimal initiator |
| KR102638006B1 (ko) * | 2016-09-26 | 2024-02-20 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| DK3929304T3 (da) * | 2016-12-16 | 2024-04-08 | Aratome Llc | Molekylær detektering ved ligeringsforstærkning |
-
2017
- 2017-12-15 DK DK21177377.5T patent/DK3929304T3/da active
- 2017-12-15 EP EP17881278.0A patent/EP3555313B1/en active Active
- 2017-12-15 CN CN201780086781.2A patent/CN110300806B/zh active Active
- 2017-12-15 EP EP21177377.5A patent/EP3929304B1/en active Active
- 2017-12-15 WO PCT/US2017/066841 patent/WO2018112422A1/en not_active Ceased
- 2017-12-15 FI FIEP21177377.5T patent/FI3929304T3/fi active
- 2017-12-15 JP JP2019531917A patent/JP7093355B2/ja active Active
- 2017-12-15 DK DK17881278.0T patent/DK3555313T3/da active
- 2017-12-15 CA CA3047127A patent/CA3047127A1/en active Pending
- 2017-12-15 CN CN202410215614.5A patent/CN118086461A/zh active Pending
- 2017-12-15 EP EP23190368.3A patent/EP4328323A3/en active Pending
-
2019
- 2019-01-23 US US16/254,926 patent/US10781477B2/en active Active
-
2020
- 2020-07-27 US US16/939,921 patent/US20200377933A1/en active Pending
-
2022
- 2022-02-14 JP JP2022020366A patent/JP2022058977A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022058977A (ja) | 2022-04-12 |
| EP3555313B1 (en) | 2021-07-21 |
| US20190153522A1 (en) | 2019-05-23 |
| EP3555313A4 (en) | 2020-08-12 |
| JP2020516232A (ja) | 2020-06-11 |
| EP4328323A3 (en) | 2024-06-12 |
| EP4328323A2 (en) | 2024-02-28 |
| WO2018112422A1 (en) | 2018-06-21 |
| EP3555313A1 (en) | 2019-10-23 |
| EP3929304A1 (en) | 2021-12-29 |
| CN110300806B (zh) | 2024-03-19 |
| DK3929304T3 (da) | 2024-04-08 |
| US10781477B2 (en) | 2020-09-22 |
| US20200377933A1 (en) | 2020-12-03 |
| EP3929304B1 (en) | 2024-02-21 |
| CN110300806A (zh) | 2019-10-01 |
| CA3047127A1 (en) | 2018-06-21 |
| JP7093355B2 (ja) | 2022-06-29 |
| DK3555313T3 (da) | 2021-08-09 |
| CN118086461A (zh) | 2024-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhao et al. | A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes | |
| Yao et al. | Specific and simultaneous detection of micro RNA 21 and let-7a by rolling circle amplification combined with lateral flow strip | |
| FI3929304T3 (fi) | Molekulaarin tunnistaminen käyttämällä ligatiovahvistusta | |
| JP2020530779A5 (enExample) | ||
| Jiang et al. | Amplified detection of DNA ligase and polynucleotide kinase/phosphatase on the basis of enrichment of catalytic G-quadruplex DNAzyme by rolling circle amplification | |
| Garafutdinov et al. | Rolling circle amplification as a universal method for the analysis of a wide range of biological targets | |
| Andersson et al. | Properties of targeted preamplification in DNA and cDNA quantification | |
| Kudryashova et al. | Preparation of mononucleosomal templates for analysis of transcription with RNA polymerase using spFRET | |
| Xiang et al. | Real-time monitoring of mycobacterium genomic DNA with target-primed rolling circle amplification by a Au nanoparticle-embedded SPR biosensor | |
| HRP20171356T1 (hr) | Izotermičko pojačavanje nukleinske kiseline | |
| Bi et al. | Dumbbell probe-mediated cascade isothermal amplification: a novel strategy for label-free detection of microRNAs and its application to real sample assay | |
| Xu et al. | An aptasensor for staphylococcus aureus based on nicking enzyme amplification reaction and rolling circle amplification | |
| Du et al. | Multiplexed aptasensing of food contaminants by using terminal deoxynucleotidyl transferase-produced primer-triggered rolling circle amplification: application to the colorimetric determination of enrofloxacin, lead (II), Escherichia coli O157: H7 and tropomyosin | |
| Zhu et al. | Label‐Free Detection of MicroRNA: Two‐Step Signal Enhancement with a Hairpin‐Probe‐Based Graphene Fluorescence Switch and Isothermal Amplification | |
| WO2007115242A3 (en) | Binary probes for fluorescent analysis of nucleic acids | |
| Guo et al. | Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes | |
| CA2692633A1 (en) | Method for the simultaneous detection of multiple nucleic acid sequences in a sample | |
| Wang et al. | Target-fueled catalytic hairpin assembly for sensitive and multiplex microRNA detection | |
| Ying et al. | Light-up RNA aptamer enabled label-free protein detection via a proximity induced transcription assay | |
| Seok et al. | Colorimetric detection of PCR products of DNA from pathogenic bacterial targets based on a simultaneously amplified DNAzyme | |
| Zhao et al. | G-quadruplex− based homogenous fluorescence platform for ultrasensitive DNA detection through isothermal cycling and cascade signal amplification | |
| Wang et al. | Colorimetric biosensor based on a DNAzyme primer and its application in logic gate operations for DNA screening | |
| Zhai et al. | A visual CRISPR/dCas9-mediated enzyme-linked immunosorbent assay for nucleic acid detection with single-base specificity | |
| JP2021502825A5 (enExample) | ||
| WO2017043114A1 (ja) | 等温増幅反応産物の多項目同時検出方法 |