JP2021502825A5 - - Google Patents
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- JP2021502825A5 JP2021502825A5 JP2020544379A JP2020544379A JP2021502825A5 JP 2021502825 A5 JP2021502825 A5 JP 2021502825A5 JP 2020544379 A JP2020544379 A JP 2020544379A JP 2020544379 A JP2020544379 A JP 2020544379A JP 2021502825 A5 JP2021502825 A5 JP 2021502825A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- amplification
- reaction
- acid sequence
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 150000007523 nucleic acids Chemical group 0.000 claims description 309
- 230000003321 amplification Effects 0.000 claims description 228
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 228
- 238000000034 method Methods 0.000 claims description 190
- 238000006243 chemical reaction Methods 0.000 claims description 189
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 139
- 108020004707 nucleic acids Proteins 0.000 claims description 135
- 102000039446 nucleic acids Human genes 0.000 claims description 135
- 239000000523 sample Substances 0.000 claims description 109
- 238000003556 assay Methods 0.000 claims description 80
- 108090000623 proteins and genes Proteins 0.000 claims description 77
- 239000002773 nucleotide Substances 0.000 claims description 64
- 125000003729 nucleotide group Chemical group 0.000 claims description 64
- 239000000203 mixture Substances 0.000 claims description 59
- 244000005700 microbiome Species 0.000 claims description 47
- 108091093088 Amplicon Proteins 0.000 claims description 33
- 239000011541 reaction mixture Substances 0.000 claims description 17
- 239000002299 complementary DNA Substances 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 10
- 239000000090 biomarker Substances 0.000 claims description 8
- 239000012472 biological sample Substances 0.000 claims description 6
- 238000002493 microarray Methods 0.000 claims description 3
- 238000009015 Human TaqMan MicroRNA Assay kit Methods 0.000 claims description 2
- 208000019206 urinary tract infection Diseases 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 description 28
- 230000000295 complement effect Effects 0.000 description 16
- 230000008685 targeting Effects 0.000 description 15
- 230000015572 biosynthetic process Effects 0.000 description 14
- 239000013612 plasmid Substances 0.000 description 10
- 241000588915 Klebsiella aerogenes Species 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 210000002700 urine Anatomy 0.000 description 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- 108700005443 Microbial Genes Proteins 0.000 description 6
- 108010010677 Phosphodiesterase I Proteins 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 6
- 108091008053 gene clusters Proteins 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 108020004999 messenger RNA Proteins 0.000 description 6
- 108091070501 miRNA Proteins 0.000 description 6
- 239000002679 microRNA Substances 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 241000588626 Acinetobacter baumannii Species 0.000 description 4
- 241000222122 Candida albicans Species 0.000 description 4
- 241000588919 Citrobacter freundii Species 0.000 description 4
- 108010041052 DNA Topoisomerase IV Proteins 0.000 description 4
- 241000588697 Enterobacter cloacae Species 0.000 description 4
- 241000194032 Enterococcus faecalis Species 0.000 description 4
- 241000194031 Enterococcus faecium Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 241000588749 Klebsiella oxytoca Species 0.000 description 4
- 241000588747 Klebsiella pneumoniae Species 0.000 description 4
- 241000588772 Morganella morganii Species 0.000 description 4
- 241000588770 Proteus mirabilis Species 0.000 description 4
- 241000588767 Proteus vulgaris Species 0.000 description 4
- 241000588778 Providencia stuartii Species 0.000 description 4
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 4
- 241001147691 Staphylococcus saprophyticus Species 0.000 description 4
- 241000193985 Streptococcus agalactiae Species 0.000 description 4
- 239000011230 binding agent Substances 0.000 description 4
- 229940095731 candida albicans Drugs 0.000 description 4
- 229940092559 enterobacter aerogenes Drugs 0.000 description 4
- 229940032049 enterococcus faecalis Drugs 0.000 description 4
- 229940076266 morganella morganii Drugs 0.000 description 4
- 229940007042 proteus vulgaris Drugs 0.000 description 4
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 4
- 108091023040 Transcription factor Proteins 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 101100002068 Bacillus subtilis (strain 168) araR gene Proteins 0.000 description 2
- 101001015517 Betula pendula Germin-like protein 1 Proteins 0.000 description 2
- 108010078791 Carrier Proteins Proteins 0.000 description 2
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 description 2
- 102000005731 Glucose-6-phosphate isomerase Human genes 0.000 description 2
- 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 2
- 108010014095 Histidine decarboxylase Proteins 0.000 description 2
- 102100037095 Histidine decarboxylase Human genes 0.000 description 2
- 101150085452 IPT1 gene Proteins 0.000 description 2
- 102000005298 Iron-Sulfur Proteins Human genes 0.000 description 2
- 108010081409 Iron-Sulfur Proteins Proteins 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 108010068005 Oxalate decarboxylase Proteins 0.000 description 2
- 102000016812 Radical SAM Human genes 0.000 description 2
- 108050006523 Radical SAM Proteins 0.000 description 2
- 102000005262 Sulfatase Human genes 0.000 description 2
- 102000003929 Transaminases Human genes 0.000 description 2
- 108090000340 Transaminases Proteins 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 101150044616 araC gene Proteins 0.000 description 2
- 101150026645 cdaR gene Proteins 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 2
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 2
- 229960001327 pyridoxal phosphate Drugs 0.000 description 2
- 108060007951 sulfatase Proteins 0.000 description 2
- 101150017526 ureR gene Proteins 0.000 description 2
- 102100028875 Formylglycine-generating enzyme Human genes 0.000 description 1
- 101000648611 Homo sapiens Formylglycine-generating enzyme Proteins 0.000 description 1
- 102100022888 KN motif and ankyrin repeat domain-containing protein 2 Human genes 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 101150106042 SUMF1 gene Proteins 0.000 description 1
- 101150117115 V gene Proteins 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023131862A JP2023175685A (ja) | 2017-11-13 | 2023-08-14 | 尿路微生物検出のための組成物、方法、およびキット |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762585273P | 2017-11-13 | 2017-11-13 | |
| US62/585,273 | 2017-11-13 | ||
| PCT/US2018/060840 WO2019094973A1 (en) | 2017-11-13 | 2018-11-13 | Compositions, methods and kits for urinary tract microorganism detection |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023131862A Division JP2023175685A (ja) | 2017-11-13 | 2023-08-14 | 尿路微生物検出のための組成物、方法、およびキット |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2021502825A JP2021502825A (ja) | 2021-02-04 |
| JP2021502825A5 true JP2021502825A5 (enExample) | 2021-12-23 |
Family
ID=64572538
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020544379A Pending JP2021502825A (ja) | 2017-11-13 | 2018-11-13 | 尿路微生物検出のための組成物、方法、およびキット |
| JP2023131862A Pending JP2023175685A (ja) | 2017-11-13 | 2023-08-14 | 尿路微生物検出のための組成物、方法、およびキット |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023131862A Pending JP2023175685A (ja) | 2017-11-13 | 2023-08-14 | 尿路微生物検出のための組成物、方法、およびキット |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20200340041A1 (enExample) |
| EP (1) | EP3710601A1 (enExample) |
| JP (2) | JP2021502825A (enExample) |
| KR (1) | KR20200081476A (enExample) |
| CN (1) | CN111527216A (enExample) |
| WO (1) | WO2019094973A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111560313A (zh) * | 2020-05-14 | 2020-08-21 | 保睿(上海)生物科技有限公司 | 一种用于肠菌微生物的检测试剂盒及其检测方法 |
| CN114410693B (zh) * | 2021-12-08 | 2023-08-04 | 中南大学 | 生物铁锰氧化物及其制备方法和在同步去除废水中的砷和锑中的应用 |
| CN119061169A (zh) * | 2023-05-31 | 2024-12-03 | 华南农业大学 | 一种桑细菌性枯萎病的检测诊断方法及检测诊断产品 |
| US20250191691A1 (en) * | 2023-12-07 | 2025-06-12 | California Institute Of Technology | Methods for the translated alignment of transcriptomic data at single-cell resolution |
| WO2025217501A1 (en) * | 2024-04-12 | 2025-10-16 | Life Technologies Corporation | Panel for detecting wound pathogens |
Family Cites Families (60)
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| CA1190838A (en) | 1981-07-17 | 1985-07-23 | Cavit Akin | Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer |
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| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US6127155A (en) | 1986-08-22 | 2000-10-03 | Roche Molecular Systems, Inc. | Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US5244797B1 (en) | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
| US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| US5047342A (en) | 1989-08-10 | 1991-09-10 | Life Technologies, Inc. | Cloning and expression of T5 DNA polymerase |
| US5270179A (en) | 1989-08-10 | 1993-12-14 | Life Technologies, Inc. | Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity |
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| EP0550687B1 (en) | 1990-09-28 | 1999-06-09 | F. Hoffmann-La Roche Ag | 5' to 3' exonuclease mutations of thermostable dna polymerases |
| DE553264T1 (de) | 1990-10-05 | 1994-04-28 | Wayne M Barnes | Thermostabile dna polymerase. |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
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| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
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| US5767259A (en) | 1994-12-27 | 1998-06-16 | Naxcor | Oligonucleotides containing base-free linking groups with photoactivatable side chains |
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| WO2017218938A1 (en) * | 2016-06-16 | 2017-12-21 | Life Technologies Corporation | Novel compositions, methods and kits for microorganism detection |
| US11613777B2 (en) * | 2016-08-26 | 2023-03-28 | Life Technologies Corporation | Nucleic acid extraction and amplification controls and methods of use thereof |
| KR101850854B1 (ko) * | 2016-10-17 | 2018-04-20 | 연세대학교 원주산학협력단 | 퀀타매트릭스 어세이 플랫폼 기반 그람양성, 그람음성균 및 캔디다의 검출 및 동정과 항생제 내성여부를 동시 확인할 수 있는 진단법 및 그 키트 |
-
2018
- 2018-11-13 WO PCT/US2018/060840 patent/WO2019094973A1/en not_active Ceased
- 2018-11-13 EP EP18812508.2A patent/EP3710601A1/en active Pending
- 2018-11-13 US US16/763,583 patent/US20200340041A1/en not_active Abandoned
- 2018-11-13 CN CN201880083733.2A patent/CN111527216A/zh active Pending
- 2018-11-13 JP JP2020544379A patent/JP2021502825A/ja active Pending
- 2018-11-13 KR KR1020207016585A patent/KR20200081476A/ko not_active Ceased
-
2023
- 2023-08-14 JP JP2023131862A patent/JP2023175685A/ja active Pending
-
2025
- 2025-05-06 US US19/199,621 patent/US20250333803A1/en active Pending
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