JP2020514746A - タンパク質をアッセイする方法 - Google Patents

タンパク質をアッセイする方法 Download PDF

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Publication number
JP2020514746A
JP2020514746A JP2019550552A JP2019550552A JP2020514746A JP 2020514746 A JP2020514746 A JP 2020514746A JP 2019550552 A JP2019550552 A JP 2019550552A JP 2019550552 A JP2019550552 A JP 2019550552A JP 2020514746 A JP2020514746 A JP 2020514746A
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protein
proteins
substrate
affinity reagents
affinity
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JP2020514746A5 (enExample
Inventor
パラグ マリック
パラグ マリック
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ノーティラス バイオテクノロジー インコーポレイテッド
ノーティラス バイオテクノロジー インコーポレイテッド
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Publication of JP2020514746A publication Critical patent/JP2020514746A/ja
Publication of JP2020514746A5 publication Critical patent/JP2020514746A5/ja
Priority to JP2022206984A priority Critical patent/JP2023030135A/ja
Priority to JP2024150202A priority patent/JP2024170480A/ja
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/10Oligonucleotides as tagging agents for labelling antibodies

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2019550552A 2016-12-01 2017-12-01 タンパク質をアッセイする方法 Pending JP2020514746A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022206984A JP2023030135A (ja) 2016-12-01 2022-12-23 タンパク質をアッセイする方法
JP2024150202A JP2024170480A (ja) 2016-12-01 2024-08-30 タンパク質をアッセイする方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662429063P 2016-12-01 2016-12-01
US62/429,063 2016-12-01
US201762500455P 2017-05-02 2017-05-02
US62/500,455 2017-05-02
PCT/US2017/064322 WO2018102759A1 (en) 2016-12-01 2017-12-01 Methods of assaying proteins

Related Child Applications (1)

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JP2022206984A Division JP2023030135A (ja) 2016-12-01 2022-12-23 タンパク質をアッセイする方法

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JP2020514746A true JP2020514746A (ja) 2020-05-21
JP2020514746A5 JP2020514746A5 (enExample) 2021-01-21

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JP2019550552A Pending JP2020514746A (ja) 2016-12-01 2017-12-01 タンパク質をアッセイする方法
JP2022206984A Pending JP2023030135A (ja) 2016-12-01 2022-12-23 タンパク質をアッセイする方法
JP2024150202A Pending JP2024170480A (ja) 2016-12-01 2024-08-30 タンパク質をアッセイする方法

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JP2022206984A Pending JP2023030135A (ja) 2016-12-01 2022-12-23 タンパク質をアッセイする方法
JP2024150202A Pending JP2024170480A (ja) 2016-12-01 2024-08-30 タンパク質をアッセイする方法

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US (14) US10473654B1 (enExample)
EP (3) EP4365597B1 (enExample)
JP (3) JP2020514746A (enExample)
CN (1) CN110249082B (enExample)
DK (1) DK3548652T3 (enExample)
WO (1) WO2018102759A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220010327A (ko) * 2020-07-17 2022-01-25 주식회사 아론티어 3d-컨벌루션 뉴럴 네트워크의 앙상블을 이용한 단백질-리간드 결합 친화도 예측 방법 및 이를 위한 시스템
JP2024514405A (ja) * 2021-03-11 2024-04-02 ノーティラス・サブシディアリー・インコーポレイテッド 生体分子保持のためのシステム及び方法
JP2024537689A (ja) * 2021-09-22 2024-10-16 ノーティラス・サブシディアリー・インコーポレイテッド ポリペプチド相互作用を決定するための方法及びシステム

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
WO2016069124A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
DE112012002570B4 (de) 2011-06-23 2023-11-23 Board Of Regents, The University Of Texas System Identifizieren von Peptiden auf der Einzelmolekülebene
DK3548652T3 (da) 2016-12-01 2024-07-08 Nautilus Subsidiary Inc Fremgangsmåder til proteinanalyse
WO2019036055A2 (en) * 2017-08-18 2019-02-21 Ignite Biosciences, Inc. METHODS OF SELECTING BINDING REAGENTS
EP3701066B1 (en) * 2017-10-23 2024-10-16 Nautilus Subsidiary, Inc. Methods and systems for protein identification
US11721412B2 (en) 2017-10-23 2023-08-08 Nautilus Subsidiary, Inc. Methods for identifying a protein in a sample of unknown proteins
AU2018358057B2 (en) 2017-10-31 2023-03-02 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
IL326759A (en) 2017-12-29 2026-04-01 Nautilus Subsidiary Inc Decoding approaches for protein identification
CN112236528A (zh) 2018-04-04 2021-01-15 诺迪勒思生物科技公司 产生纳米阵列和微阵列的方法
CA3110800A1 (en) 2018-07-12 2020-01-16 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
CN118050525A (zh) 2018-08-23 2024-05-17 普诺森公司 用于检测和分析自由硫醇的系统和方法
CA3117476A1 (en) 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
GB2595583A (en) 2018-11-07 2021-12-01 Seer Inc Compositions, methods and systems for protein corona analysis and uses thereof
US11993865B2 (en) 2018-11-20 2024-05-28 Nautilus Subsidiary, Inc. Selection of affinity reagents
CA3131402A1 (en) 2019-03-26 2020-10-01 Xiaoyan Zhao Compositions, methods and systems for protein corona analysis from biofluids and uses thereof
EP3963091A4 (en) 2019-04-29 2023-07-19 Nautilus Biotechnology, Inc. METHODS AND SYSTEMS FOR ON-CHIP INTEGRATED SINGLE MOLECULE DETECTION
WO2021087402A1 (en) 2019-10-30 2021-05-06 Nautilus Biotechnology, Inc. Flow cell systems and methods
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
WO2021163374A2 (en) * 2020-02-12 2021-08-19 Becton, Dickinson And Company Intracellular abseq
CN111289425B (zh) * 2020-03-12 2021-03-30 中国计量科学研究院 基于荧光标记流式单分子计数的蛋白质含量计量基准方法
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
JP2023540904A (ja) 2020-08-25 2023-09-27 シアー, インコーポレイテッド タンパク質および核酸をアッセイするための組成物および方法
US11880345B2 (en) * 2020-09-14 2024-01-23 Tata Consultancy Services Limited Method and system for generating annotations and field-names for relational schema
KR20230118570A (ko) 2020-11-11 2023-08-11 노틸러스 서브시디어리, 인크. 강화된 결합 및 검출 특성을 갖는 친화성 시약
US12092642B2 (en) 2021-01-20 2024-09-17 Nautilus Subsidiary, Inc. Systems and methods for biomolecule quantitation
EP4281775B1 (en) 2021-01-21 2026-04-29 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
CN117413183A (zh) * 2021-03-11 2024-01-16 诺迪勒思附属公司 用于生物分子保留的系统和方法
WO2022271983A1 (en) 2021-06-24 2022-12-29 Nautilus Biotechnology, Inc. Methods and systems for assay refinement
CA3227872A1 (en) 2021-09-09 2023-03-16 James Henry JOLY Characterization and localization of protein modifications
US20230114905A1 (en) 2021-10-11 2023-04-13 Nautilus Biotechnology, Inc. Highly multiplexable analysis of proteins and proteomes
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
US12474346B2 (en) 2021-11-05 2025-11-18 Encodia, Inc. Methods and kits for multicycle encoding assay
US12612656B2 (en) 2021-11-30 2026-04-28 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
WO2023122589A2 (en) 2021-12-22 2023-06-29 Nautilus Subsidiary, Inc. Systems and methods for carrying out highly multiplexed bioanalyses
CN114388058A (zh) * 2022-01-13 2022-04-22 西湖大学 基于九轴imu的蛋白任意截面生成方法
CN114388060B (zh) * 2022-01-13 2023-06-20 西湖大学 基于圆形控制器的蛋白球形截面生成方法
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
NL2032916B1 (en) 2022-08-31 2024-03-15 Univ Delft Tech Single-molecule aptamer FRET for protein identification and structural analysis
WO2024058967A1 (en) 2022-09-13 2024-03-21 Nautilus Subsidiary, Inc. Systems and methods of validating new affinity reagents
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
EP4594519A1 (en) 2022-09-27 2025-08-06 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
EP4619766A1 (en) 2022-11-15 2025-09-24 Nautilus Subsidiary, Inc. Standard polypeptides
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
WO2024130000A1 (en) 2022-12-15 2024-06-20 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
WO2024151373A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
EP4666071A2 (en) 2023-02-13 2025-12-24 Nautilus Subsidiary, Inc. Affinity reagents having artificial polymer scaffolds
US20240301469A1 (en) 2023-02-24 2024-09-12 Nautilus Subsidiary, Inc. Modifying, separating and detecting proteoforms
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
US20240353416A1 (en) 2023-04-13 2024-10-24 Nautilus Subsidiary, Inc. Artificial proteins for displaying epitopes
EP4720641A1 (en) 2023-05-24 2026-04-08 Nautilus Subsidiary, Inc. Systems and methods for machine vision
EP4728099A2 (en) 2023-06-14 2026-04-22 Nautilus Subsidiary, Inc. Making and using structured nucleic acid particles
EP4732011A1 (en) 2023-06-21 2026-04-29 Nautilus Subsidiary, Inc. Compositions and methods for improving affinity reagent avidity
NL2035603B1 (en) 2023-08-14 2025-02-25 Univ Delft Tech Proximity labelling and FRET-based protein fingerprinting
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025128588A1 (en) 2023-12-11 2025-06-19 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
WO2025199236A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
US20250306015A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Single molecule-resolved characterization of affinity reagent kinetics and thermodynamics
US20250354988A1 (en) 2024-05-14 2025-11-20 Nautilus Subsidiary, Inc. Time-dependent profiling of binding interactions
WO2025255117A1 (en) 2024-06-06 2025-12-11 Nautilus Subsidiary, Inc. Analyte characterization by differential binding of binding reagents
WO2026006467A1 (en) 2024-06-27 2026-01-02 Nautilus Subsidiary, Inc. Enrichment of particle reagents for array-based assays
WO2026024985A1 (en) 2024-07-26 2026-01-29 Nautilus Subsidiary, Inc. Methods and systems for characterizing proteoforms of significant proteins of interest
US20260092914A1 (en) 2024-09-27 2026-04-02 Nautilus Subsidiary, Inc. Single analyte characterization under equilibrium binding conditions
WO2026084863A1 (en) 2024-10-17 2026-04-23 Nautilus Subsidiary, Inc. Analyte characterization via iterative analysis

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055186A1 (en) * 2000-09-19 2002-05-09 Oxford Glycosciences (Uk) Ltd. Detection of peptides
US20030054408A1 (en) * 2001-04-20 2003-03-20 Ramamoorthi Ravi Methods and systems for identifying proteins
JP2006503310A (ja) * 2002-10-15 2006-01-26 アブメトリックス, インコーポレイテッド 短いエピトープに対して向けられる複数組のディジタル抗体、およびその使用
US20060160234A1 (en) * 2005-01-18 2006-07-20 Viorica Lopez-Avila Methods for assessing polypeptide array quality
JP2007071645A (ja) * 2005-09-06 2007-03-22 Sharp Corp タンパク質の測定方法
US20070218503A1 (en) * 2006-02-13 2007-09-20 Mitra Robi D Methods of polypeptide identification, and compositions therefor

Family Cites Families (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1017524A (en) 1911-05-01 1912-02-13 Robert George Ferguson Flush-tank.
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5635602A (en) 1993-08-13 1997-06-03 The Regents Of The University Of California Design and synthesis of bispecific DNA-antibody conjugates
US6682886B1 (en) 1994-04-28 2004-01-27 Gilead Sciences, Inc. Bivalent binding molecules of 7 transmembrane G protein-coupled receptors
SE9601676D0 (sv) 1996-04-30 1996-04-30 Ulf Landegren Improved probing of specific mucleic acids
US6762056B1 (en) 1997-11-12 2004-07-13 Protiveris, Inc. Rapid method for determining potential binding sites of a protein
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US6489608B1 (en) 1999-04-06 2002-12-03 Micromass Limited Method of determining peptide sequences by mass spectrometry
WO2000073787A1 (en) 1999-05-27 2000-12-07 Rockefeller University An expert system for protein identification using mass spectrometric information combined with database searching
WO2001046675A2 (en) 1999-12-23 2001-06-28 Illumina, Inc. Decoding of array sensors with microspheres
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
WO2002071067A1 (en) * 2001-03-07 2002-09-12 Bio-Rad Laboratories, Inc. Assay system for simultaneous detection and measurement of multiple modified cellular proteins
US20050074809A1 (en) 2001-03-10 2005-04-07 Vladimir Brusic System and method for systematic prediction of ligand/receptor activity
US6720595B2 (en) 2001-08-06 2004-04-13 International Business Machines Corporation Three-dimensional island pixel photo-sensor
WO2003044231A1 (en) 2001-11-23 2003-05-30 Simon Fredriksson Method and kit for proximity probing with multivalent proximity probes
AU2002351164A1 (en) 2001-11-26 2003-06-10 Molecular Reflections, Inc. Microscale immobilization of molecules using a hydrogel and methods of use thereof
US20040067599A1 (en) * 2001-12-14 2004-04-08 Katz Joseph L. Separation identification and quantitation of protein mixtures
WO2003083441A2 (en) 2002-03-29 2003-10-09 Buck Institute Identification of apoptotic peptides and methods of use thereof
US6998241B2 (en) 2002-09-11 2006-02-14 Kimberly-Clark Worldwide, Inc. Antibody pair screening methods
CN101410530B (zh) 2003-04-18 2013-03-27 贝克顿·迪金森公司 免疫-扩增
US20040265938A1 (en) 2003-06-25 2004-12-30 Jose Remacle Method for the determination of cell activation
JP3788513B2 (ja) * 2003-09-11 2006-06-21 セイコーエプソン株式会社 固相基板上への分子の固定化方法およびそれを用いるバイオセンサの製造方法
US7593817B2 (en) 2003-12-16 2009-09-22 Thermo Finnigan Llc Calculating confidence levels for peptide and protein identification
US20050148100A1 (en) * 2003-12-30 2005-07-07 Intel Corporation Methods and devices for using Raman-active probe constructs to assay biological samples
EP1866326A4 (en) 2005-03-17 2010-06-16 Primex Clinical Lab Inc IMMUNOGENES FOR VACCINES AGAINST ANTIGENVARIABLE PATHOGENES AND DISEASES
WO2006122002A2 (en) 2005-05-09 2006-11-16 Panomics, Inc. Multiplex capture of nucleic acids
CA2611198C (en) 2005-06-10 2015-04-28 Saint Louis University Methods for the selection of aptamers
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
EP1941276A1 (de) 2005-10-29 2008-07-09 Bayer Technology Services GmbH Verfahren zur bestimmung eines oder mehrerer analyten in komplex zusammengesetzten proben biologischen ursprungs und dessen verwendung
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
US7692783B2 (en) 2006-02-13 2010-04-06 Pacific Biosciences Of California Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
WO2007117444A2 (en) 2006-03-31 2007-10-18 Yinghe Hu Protein detection by aptamers
CN100500865C (zh) 2006-04-21 2009-06-17 华东师范大学 一种通过同时识别多个表位来检测CatD蛋白的方法
US7855054B2 (en) 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20110136099A1 (en) 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7964356B2 (en) 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
AU2008237018B2 (en) 2007-04-10 2014-04-03 Bruker Spatial Biology, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
US8404830B2 (en) 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8853185B2 (en) 2008-04-09 2014-10-07 Cornell University Coferons and methods of making and using them
US9340416B2 (en) 2008-08-13 2016-05-17 California Institute Of Technology Polynucleotides and related nanoassemblies, structures, arrangements, methods and systems
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
WO2010065531A1 (en) * 2008-12-01 2010-06-10 Robi David Mitra Single molecule protein screening
WO2010111691A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Conjugates of biomolecules to nanoparticles
US9532956B2 (en) 2009-04-18 2017-01-03 Massachusetts Institute Of Technology PH sensitive biodegradable polymeric particles for drug delivery
BR112012017483A2 (pt) 2010-01-14 2019-09-24 Haplomics Inc previsão e redução de aloimunogenicidade de terapêuticos de proteína
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
AU2011240774B2 (en) 2010-04-12 2014-04-10 Otsuka Pharmaceutical Co., Ltd. Aptamers to beta-NGF and their use in treating beta-NGF mediated diseases and disorders
US8774494B2 (en) * 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US9523680B2 (en) 2010-06-30 2016-12-20 Ambergen, Inc. Global Proteomic screening of random bead arrays using mass spectrometry imaging
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
CN102618940A (zh) 2011-01-31 2012-08-01 艾比玛特生物医药(上海)有限公司 抗体制备方法及所得抗体和抗体库
US20130053541A1 (en) 2011-03-11 2013-02-28 Lynntech, Inc. Methods for discovering molecules that bind to proteins
DE112012002570B4 (de) 2011-06-23 2023-11-23 Board Of Regents, The University Of Texas System Identifizieren von Peptiden auf der Einzelmolekülebene
WO2016069124A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
US9410156B2 (en) 2012-03-28 2016-08-09 Somalogic, Inc. Aptamers to PDGF and VEGF and their use in treating PDGF and VEGF mediated conditions
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
DK2920725T3 (da) * 2012-11-19 2022-02-07 Apton Biosystems Inc Digital analyse af molekylære analytter ved hjælp af enkeltmolekyledetektering
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
AU2014265353A1 (en) * 2013-05-16 2015-11-12 Unimed Biotech (Shanghai) Co., Ltd. Fetal diagnostics using fetal cell capture from maternal blood
US9368187B2 (en) * 2013-07-11 2016-06-14 Qualcomm Incorporated Insertion-override counter to support multiple memory refresh rates
KR102348283B1 (ko) 2013-09-24 2022-01-10 소마로직, 인크. 멀티앱타머 표적 검출
SG10202006426QA (en) 2013-11-21 2020-08-28 Somalogic Inc Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
US10161938B2 (en) 2013-12-27 2018-12-25 Roche Sequencing Solutions, Inc. Systematic discovery, maturation and extension of peptide binders to proteins
EP3009520B1 (en) 2014-10-14 2018-12-12 Karlsruher Institut für Technologie Site-specific immobilization of DNA origami structures on solid substrates
EP3274473B1 (en) 2015-03-24 2020-10-28 Pacific Biosciences of California, Inc. Methods and compositions for single molecule composition loading
US9881786B2 (en) 2015-04-02 2018-01-30 Micron Technology, Inc. Methods of forming nanostructures using self-assembled nucleic acids, and nanostructures thereof
WO2016174525A1 (en) 2015-04-29 2016-11-03 Biosims Technologies Enhanced sensitivity in ligand binding assays performed with secondary ion mass spectrometry
EP3298135B1 (en) 2015-05-18 2023-06-07 The Regents of the University of California Systems and methods for predicting glycosylation on proteins
EP3405575A4 (en) 2016-01-20 2019-06-26 Vitrisa Therapeutics, Inc. PROCESS FOR IMPROVED APTAMER SELECTION
EP4299803A3 (en) 2016-05-02 2024-03-27 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
DK3548652T3 (da) 2016-12-01 2024-07-08 Nautilus Subsidiary Inc Fremgangsmåder til proteinanalyse
WO2019036055A2 (en) 2017-08-18 2019-02-21 Ignite Biosciences, Inc. METHODS OF SELECTING BINDING REAGENTS
US11125748B2 (en) 2017-09-01 2021-09-21 University Of British Columbia Method for organizing individual molecules on a patterned substrate and structures assembled thereby
US11391734B2 (en) 2017-09-25 2022-07-19 California Institute Of Technology Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events
US11721412B2 (en) 2017-10-23 2023-08-08 Nautilus Subsidiary, Inc. Methods for identifying a protein in a sample of unknown proteins
EP3701066B1 (en) 2017-10-23 2024-10-16 Nautilus Subsidiary, Inc. Methods and systems for protein identification
WO2019089846A1 (en) 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
SG11202003923PA (en) 2017-10-31 2020-05-28 Encodia Inc Methods and kits using nucleic acid encoding and/or label
AU2018358057B2 (en) 2017-10-31 2023-03-02 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
IL326759A (en) 2017-12-29 2026-04-01 Nautilus Subsidiary Inc Decoding approaches for protein identification
CN112236528A (zh) 2018-04-04 2021-01-15 诺迪勒思生物科技公司 产生纳米阵列和微阵列的方法
US20210239705A1 (en) 2018-06-06 2021-08-05 Nautilus Biotechnology, Inc. Methods and applications of protein identification
US11993865B2 (en) 2018-11-20 2024-05-28 Nautilus Subsidiary, Inc. Selection of affinity reagents
EP3963091A4 (en) 2019-04-29 2023-07-19 Nautilus Biotechnology, Inc. METHODS AND SYSTEMS FOR ON-CHIP INTEGRATED SINGLE MOLECULE DETECTION
WO2021003470A1 (en) 2019-07-03 2021-01-07 Nautilus Biotechnology, Inc. Decoding approaches for protein and peptide identification
WO2021087402A1 (en) 2019-10-30 2021-05-06 Nautilus Biotechnology, Inc. Flow cell systems and methods
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
US12092642B2 (en) 2021-01-20 2024-09-17 Nautilus Subsidiary, Inc. Systems and methods for biomolecule quantitation

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055186A1 (en) * 2000-09-19 2002-05-09 Oxford Glycosciences (Uk) Ltd. Detection of peptides
US20030054408A1 (en) * 2001-04-20 2003-03-20 Ramamoorthi Ravi Methods and systems for identifying proteins
JP2006503310A (ja) * 2002-10-15 2006-01-26 アブメトリックス, インコーポレイテッド 短いエピトープに対して向けられる複数組のディジタル抗体、およびその使用
US20060160234A1 (en) * 2005-01-18 2006-07-20 Viorica Lopez-Avila Methods for assessing polypeptide array quality
JP2007071645A (ja) * 2005-09-06 2007-03-22 Sharp Corp タンパク質の測定方法
US20070218503A1 (en) * 2006-02-13 2007-09-20 Mitra Robi D Methods of polypeptide identification, and compositions therefor

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220010327A (ko) * 2020-07-17 2022-01-25 주식회사 아론티어 3d-컨벌루션 뉴럴 네트워크의 앙상블을 이용한 단백질-리간드 결합 친화도 예측 방법 및 이를 위한 시스템
KR102576033B1 (ko) 2020-07-17 2023-09-11 주식회사 아론티어 3d-컨벌루션 뉴럴 네트워크의 앙상블을 이용한 단백질-리간드 결합 친화도 예측 방법 및 이를 위한 시스템
JP2024514405A (ja) * 2021-03-11 2024-04-02 ノーティラス・サブシディアリー・インコーポレイテッド 生体分子保持のためのシステム及び方法
JP2024537689A (ja) * 2021-09-22 2024-10-16 ノーティラス・サブシディアリー・インコーポレイテッド ポリペプチド相互作用を決定するための方法及びシステム

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